Expression Rate Of P53 Protein Research Articles

Clinicopathological features of basal cell type dysplasia of esophagus

Objective: To investigate the clinicpathological features of basal cell type dysplasia of the esophagus. Methods: The clinicopathological data of 71 cases of basal cell type dysplasia of esophagus were collected at the People's Liberation Army Joint Logistics Support Force 989 Hospital, from 2009 to 2019, and the histomorphologic characteristics and immunophenotype were evaluated. The relevant literature was reviewed. Results: The ratio of male to female patients was 1.6∶1.0, and the median age was 65 years (range 48-81 years). The tumors were located in the upper segment of the esophagus in four cases (5.6%), the middle segment in 54 cases (76.1%), and the lower segment in 13 cases (18.3%).The median maximal tumor diameter was 12.0 mm (range 3-42 mm). According to Paris Classification, 0-Ⅱb accounted for 42.3% (30/71) of the cases. Under endoscope, the lesions were reddish with abnormal mucosal microvessels. Histologically, the neoplastic cells were small, with a high nuclear-cytoplasmic ratio, similar to basal cells, and uniform in morphology. The structural atypia was characterized by dense and disordered tumor cells, loss of basal cell polarity, and absence of normal squamous differentiation gradient. In 10 cases, the tumors were confined to the lower part of the epithelium. The tumor cells were smaller and more uniform in shape, and extend to the superficial lamina propria. Sixty-one tumors involved at least the entire layer of the upper cortex. There were 31 cases of neoplasms with superficial invasive carcinoma. The types of neoplasms included typical squamous cell carcinoma, basaloid squamous cell carcinoma, small cell neuroendocrine carcinoma, squamous cell carcinoma with sebaceous adenoid carcinoma, and differentiation of glandular/ductal epithelioid carcinoma. Immunohistochemical staining showed that the mutant expression rate of p53 protein was 41.5% (17/41). All 41 cases (100.0%) showed abnormal distribution pattern of Ki-67. According to the initial pathologic diagnosis, there were 18 cases of low grade dysplasia, 12 cases of atypical epithelial cells, and 41 cases of high grade dysplasia and superficially invasive carcinoma. Conclusions: Basal cell type dysplasia has unique morphologic characteristics and represents a tumor subtype in the morphologic lineage of esophageal squamous dysplasia. Tumor cells of basal cell type dysplasia, especially those distributed only in the lower part of the stratified squamous epithelium, may be tumor stem cells at the earliest stage of esophageal carcinogenesis and have multidirectional differentiation potential. When the tumor is confined to the lower part of the stratified squamous epithelium, it does not meet the diagnostic criteria for esophageal squamous dysplasia as defined by the current WHO classification.

Expressions and their Significance of Ki-67, p53, and survivin in esophageal cancer and precancerosis

Objective To investigate the expressions and clinical significance of Ki-67, p53, and survivin in esophageal cancer and precancerosis. Methods The expressions of Ki-67, p53, and survivin proteins were detected by immunohistochemical staining in 40 normal esophageal mucosa, 136 precancerosis (42 mild atypical hyperplasia, 43 moderate atypical hyperplasia, and 51 severe atypical hyperplasia), and 68 esophageal cancer tissues. The correlation of three proteins expressed in esophageal carcinoma tissues was analyzed. Results The positive expression rate of Ki-67 was 0 (0/40)for normal epithelium, 35.7% (15/42) for mild dysplasia, 51.2% (22/43) for moderate dysplasia, 74.5% (38/51) for severe dysplasia, 92.6% (63/68) for squamous carcinoma, respectively. The positive expression rate of p53 protein was 0 (0/40) for normal epithelium, 28.6% (12/42) for mild dysplasia, 46.5% (20/43) for moderate dysplasia, 52.9% (27/51) for severe dysplasia, 67.6% (46/68) for squamous carcinoma, respectively. The positive expression rate of survivin protein was 0 (0/40) for normal epithelium, 38.1% (16/42) for mild dysplasia, 55.8% (24/43) for moderate dysplasia, 64.7% (33/51) for severe dysplasia, 89.7% (61/68) for squamous carcinoma, respectively. Rank correlation analysis showed that abnormal expressions of Ki-67, p53, and survivin were correlated significantly with the pathological grading of the lesions (r=0.637, 0.454, 0.590, P<0.01). The expressions of Ki-67, p53, and survivin were positively correlated in esophageal carcinoma (r=0.407, 0.646, P<0.01). Conclusions The abnormal expressions of Ki-67, p53, and survivin were associated with the processes of the esophageal canceration, and the joint detection with three parameters has important clinical value. Key words: Esophageal neoplasms/ME; Esophageal diseases/ME; Ki-67 antigen/ME; Genes, p53; Microtubule-associated proteins/ME

Research on expression and importance of p53, p16 and VEGF-C in cervical cancer

To investigate the expression of p53, p16(INK4A) and VEGF-C proteins in cervical cancer and their clinical importance. The expression of p53, p16(INK4A) and VEGF-C proteins in 125 cases of cervical sections were detected by two-step immunohistochemical EnVision method, and analyzed combined with the clinical and pathological data. (1) The positive expression rates of p53, p16(INK4A) and VEGF-C proteins were 56.8%, 95.2% and 88%, respectively. (2) The positive expression rate of p53 protein was 75% in lymph node metastasis-positive cases and 48.24% in lymph node metastasis-negative cases (P<0.05). The presence of difference for positive significant p53 in squamous cell carcinoma and adenocarcinoma is intensively large (P<0.01), but its expression is not much influenced by age, degree of differentiation, parametric infiltration, vascular metastasis or clinical staging (P>0.05). (3) The p16(INK4A) expression in squamous cell carcinoma is 95.65%, and it is 93.94% in adenocarcinoma, the differential expression rates bears no statistically significant, but the strong expression of squamous cell carcinoma covers 55.43% of the total number of squamous cell carcinoma, while only 15.15% rates in adenocarcinoma (P<0.01). The p16(INK4A) protein expression was not significantly correlated to age, histological type, degree of differentiation, lymph node metastasis, parametric infiltration, vascular metastasis or clinical staging (P>0.05). (4) Among the cases of lymph node metastasis, there were 100% of VEGF-C-positive expression and 82.35% of VEGF-C-negative expression, and the difference was significant (P<0.05). Among the cases of vasculature-positive samples of cervical cancer, there were 98.4% of VEGF-C-positive expression; while among the vasculature-negative samples of cervical cancer, there were 76.27% of VEGF-C-positive expression; the difference was significant (P<0.01). VEGF-C protein expression was not significantly correlated to age, histological type, degree of differentiation, parametric infiltration or clinical staging (P>0.05). The expression of some immune enzymes in cervical cancer has a reference value for early diagnosis and prognosis of cervical cancer, and has a certain guiding significance for development of the treatment program. However, a larger sample is needed for supporting the research of its significance.

Experimental study of adenovirus-mediated p53 gene on the reversal of multidrug resistance in breast cancer

To explore the effects of adenovirus-mediated wild-type p53 gene (Ad-p53) on the reversal of multidrug resistance (MDR) and multidrug resistance gene-1 (MDR1) in adriamycin (ADM) resistant human breast cancer cell. Ad-p53 was transfected into the human breast cancer cells of the line MCF-7/ADM. p53 protein expression was detected by flow cytometry (FCM). The cell proliferation was assessed by trypan blue dye exclusion. The effects of Ad-p53 on ADM mediated drug resistance were observed by MTT assay. The expression levels of MDR1 mRNA were detected with real-time RT-PCR. The expression rate of p53 protein of the MCF-7/ADM cell was increased from 10.24% to 36.20% after transfected by MOI(50) Ad-p53 for 48 hours. The growth inhibition rate was 7.4 % (P = 0.003). The chemosensitivity of MCF-7/ADM to ADM increased by 11.6 times (P = 0.001). The result of real-time RT-PCR reveales that MDR1 mRNA was decreased from 1.25 +/- 0.01 to 0.91 +/- 0.01 (P = 0.011) after the MCF-7/ADM cells were transfected by Ad-p53. Ad-p53 can inhibit the expression of MDR1 gene and partially reverse the MDR of MCF-7/ADM cells, thus enhancing the chemosensitivity of human breast cancer cells to ADM.

The relationship between the p53 expression and MRI manifestion of the T-staging and the lymph nodes metastasis of the nasopharyngeal carcinoma

目的 探讨鼻咽癌T分期、淋巴结转移的MRI表现与p53蛋白表达的关系.方法 搜集首次放疗前行MR检查且临床资料完整的患者104例,用链霉菌抗生物蛋白-过氧化酶免疫组织化学(SP)法检测p53蛋白的表达,分析T分期、淋巴结转移的MRI表现与p53的关系.结果 影像学表现:104例中,经MRI分析T1、T2期各32例,T3、T4期各20例;颈部淋巴结转移49例,咽后外侧淋巴结转移34例.鼻咽癌中的p53蛋白的阳性表达率为82.7%(86/104).T3+T4组p53蛋白的阳性表达率92.5%(37/40),T1+T2组p53蛋白阳性表达率为76.6%(49/64),2组之间差异有统计学意义(χ2=4.37,P=0.037).T1、T2、T3、T4期高表达率分别为81.3%(26/32)、59.4%(19/32)、95.0%(19/20)、85.0%(17/20),p53蛋白表达强度随着MRI的T分期升高而有增加趋势(χ2=10.57,P=0.014).颈部淋巴结转移和咽后外侧淋巴结转移的p53蛋白阳性表达率分别为77.6%(38/49)、76.5%(26/34),淋巴结的转移与p53蛋白的表达情况无关联(χ2值分别为0.591、0.795,P值均＞0.05);颈部淋巴结直径≥4 cm组p53蛋白的阳性表达率81.8%(9/11),＜4 cm组阳性表达率76.3%(29/38),二者之间的差异元统计学意义(χ2=0.005,P=0.944).结论 鼻咽癌中有较高的p53表达,p53蛋白的过度表达可能与鼻咽癌的发生有关;p53蛋白的表达强度随着MRI T分期升高有增强的趋势;淋巴结的肿大与否及大小与p53蛋白的表达无关。

반전성 유두종에서 p53 유전자의 발현

Background and Objective: Inverted papilloma is benign lesion of paranasal sinuses, which occupies 0.5-4.0% of the neoplasm of nose. Pathologically, it is benign, but frequently invades surrounding tissue, resulting in recurrence. p53 gene is considered to be tumor suppressor gene. By some cause, if there is production of variant of p53, the change in cell cycle induced. In head and neck cancer, some repart variant form of p53 protein is related to progression of tumorogenesis. Human papillomavirus (HPV) is considered as a carcinogenesis of inverted papilloma. In the tumorogenesis of this virus, the mechanism of action is induction of impotence of p53 tumor suppressor gene of host. The purpose of this study is to understand the mechanism of pathogenesis and biologic characteristics of nasal inverted papilloma. Materials and Method: Immunohistochemical stain method of inverted papilloma, squamous cell ca in paranasal sinus, and hypertrophied nasal mucosa targeting of change of p53 protein. Results: The expression rates of p53 protein were respectively 80%, 17.2%, and 0% in squmous cell carcinomas, inverted papillomas, and inferior turbinate mucosas. The expression rate of p53 protein of inverted papillomas with dysplasia was higher (44.4%) than inverted papillomas without dysplasia (5%). Conclusion: By this result, the expression of p53 protein is considered to be related with malignant pontential, resulting in overmultiplication of inverted papillomas. These results showed that p53 play a role as a marker presenting malignancy of inverted papilloma. (J Clinical Otolaryngol 2001;12:229-233)

Effect of HCV NS(3) protein on p53 protein expression in hep atocarcinogenesis.

AIM:To investigate hepatocarcinogenesis by detecting the effect of HCV NS(3) protein on p53 protein expression in hepatocellular carcinoma (HCC) and pericarcinomatous liver tissue (PCLT).METHODS:The expression of HCV NS(3) and p53 protein was detected with immunohistochemical technique (SP method) in specimens of HCC and PCLT from 47 patients with negative HBV.RESULTS:The positive rate of HCV NS(3) protein was lower in HCC (62%) than in PCLT (83%) (P < 0.025). The better differentiaton of cancer cells, the stronger expression of HCV NS(3) protein (P < 0.025). The positive rate of p53 protein in HCC (81%) was higher than in PCLT (47%) (P < 0.025). The worse differentiaton of cancer cells, the stronger expression of p53 protein (P < 0.05). The p53 protein expression was not correlated with the HCV NS(3) protein expression in HCC (P < 0.5), whereas their expression was closely related to PCLT (P < 0.01), and the expression rate of p53 protein in the cases of positive HCV NS(3) protein was higher than that in the cases of negative HCV NS(3) protein.CONCLUSION:HCV NS(3) protein may exert its hepatocarcinogenic effect in early stage on host cells by endogenous pathway which may bring about mutation of p53 gene and transformation of hepatocytes.

The significance of PCNA and p53 protein in some oral tumors

The expression of PCNA and p53 protein was evaluated in a total of 75 cases of benign and malignant lesions of the oral cavity, comprising 50 squamous cell carcinomas (SCCs), 14 leukoplakias, and 11 pleomorphic adenomas. The DNA histogram of 20 SCCs was measured by flow cytometry. p53-positive cells were frequently seen in SCCs, but were rare in leukoplakias and pleomorphic adenomas. The PCNA labeling index (LI) was higher in SCCs than in other benign lesions. The expression rate of p53 protein was markedly elevated in SCCs obtained from smoking patients, when compared to nonsmoking patients. DNA ploidy did not show a close relationship with PCNA and p53 expression. The mean value of PCNA LI for 22 cases carrying positive p53 protein was 52.3%, which was higher than that of p53 protein negative cases (35.7%). The Kaplan-Meier survival curve of the patients who were negative for p53 was significantly more favorable than for patients who were positive for p53 ( P<0.01, Cox-Mantel test). These results suggest that PCNA and p53 LI are markers for the malignant potential of the oral mucosa, and are a useful indicator suggesting a poor prognosis.

High prevalence of p53 protein overexpression in patients with esophageal cancer in Linxian, China and its relationship to progression and prognosis.

Linxian is the highest endemic area of esophageal squamous cell carcinoma (ESCC) in China and one of the highest incidence areas in the world. The relationship of p53 protein accumulation to geographic variation, pathologic findings, and prognosis has not been investigated extensively. Formalin fixed, paraffin embedded ESCC tissues from 100 patients who underwent esophagectomy between 1973 and 1983 were immunostained by using monoclonal antibody pAB1801. p53 overexpression was observed in 41 (87.2%) of 47 tumors of patients in Linxian and in 16 (64%) of 25 additional patients outside Linxian. Its prevalence in the noncancerous epithelium (11/72, 15.3%) and carcinoma in situ (1/7, 14.3%) was lower than that in invasive lesions (64/93, 68.8%). Its immunostaining intensity increased with the depth of cancer invasion. Of 30 primary carcinomas with lymph node metastasis, 29 (96.7%) were positive. However, only 36 (51.4%) of 70 primary lesions without metastasis were positive, and a higher intensity was noticed in the metastases. There was a lower expression rate in tumors of patients surviving more than 10 years (25/52, 48.1%) than in those surviving less than 3 years (40/48, 83.3%). Overall and nonadvanced or metastasis-free cumulative survival rates were both significantly different in patients with and without p53 protein overexpression. There is a higher expression rate of p53 protein in ESCC in tumors of patients from Linxian than in those from the surrounding area. The accumulation of p53 protein is related to the invasiveness and capability for metastases of cancer cells and appears to be a useful prognostic factor for patients with ESCC.