AbstractThe aim of this study was to compare strategies to prevent clostridium growth in sugarcane silages added with lime. In Experiment 1, treatments were (as fed basis): no additives (Control), 15 g lime kg−1 (L), 15 g lime kg−1 + 1.5 g sodium benzoate kg−1 (L+B), 15 g lime kg−1 + 0.7 g sodium nitrite kg−1 (L+N), 15 g lime kg−1 + Lactobacillus buchneri (5 × 105 cfu g−1) (L+LB) and 15 g lime kg−1 + Lactobacillus plantarum (5 × 105 cfu g−1) (L+LP). Plastic buckets (20 L) were used as experimental silos. Silage fermentative losses and aerobic stability were also evaluated. Lime treatment was not effective in decreasing dry matter (DM) losses compared with control silage (121 and 158 g kg−1, respectively). On the other hand, L+LB, L+B, L+LP and L+N treatments significantly decreased DM losses (70, 73, 87 and 98 g kg−1, respectively). The aerobic stability of silages L (172 h), L+B (155 h) and L+N (223 h) was substantially higher compared to those treated with L+LB (49 h), L+LP (48 h) and control (52 h). Control silages had low counts of clostridia (log 3.3 cfu g−1) whereas L and L+LB treated silages reached very high clostridium counts (log 6.7 and log 6.0 cfu g−1, respectively). The remaining treatments resulted in intermediate values. In Experiment 2, sugarcane treated with lime (15 g kg−1) received the following doses of sodium nitrite prior to ensiling: 0, 0.5, 1.0 and 1.5 g kg−1 as fed. Treatments did not affect clostridium counts but decreased butyric acid and 2,3‐butanediol. The silage with the highest nitrite dose decreased the butyric acid content substantially compared to other treatments but the final level (5.9 g kg−1 DM) was above the target value for well‐fermented silage. None of additives combined with lime were able to provide completely butyric acid free silages.
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