Experimental Approach Research Articles

Studying the hydration kinetics and mechanical-microstructural properties of Portland cements made with and without dredged sediment: experimental and numerical approaches

This research focused on two objectives: (i) investigating the impacts of sediment substitution in the raw meal on the hydration and mechanical-microstructural properties of cement; (ii) assessing the reliability of CEMHYD3D code for modeling the properties of hydrated cement. The experimental results indicated that a maximum rate of sediment up to 7.55% had no impact on the formation of mineralogical phases of clinker, the hydration and mechanical-microstructural development of cement. The degree of hydration and strengths of cement made of sediment substitution were slightly higher than those of reference cement, whereas the critical diameter of pores of both hydrated cements was nearly identical. Comparing the modeling results with the experimental measurements showed good predictions for the degree of hydration, hydration heat as well as strength development. However, the formation of hemi-and mono-carboaluminate phases was not predicted in the model, and the porosity prediction was also limited to the capillary porosity.

Isotopic niches reveal the impact of topmouth gudgeon and gibel carp on native crucian carp

Invasive species pose a major threat to natural ecosystems and directly outcompete many native species, placing them at imminent threat of extinction. The topmouth gudgeon (Pseudorasbora parva) is on the EU’s blacklist of invasive freshwater species and threatens biodiversity, especially in wetland and floodplain ecosystems, aquacultures and village ponds. The crucian carp (Carassius carassius) is native to Europe and its populations have declined in large part of its native range, with invasive gibel carp (C. gibelio) suspected as a major cause of its decline. Invasions by topmouth gudgeon have been implicated in the decline of crucian carp populations but this still needs to be verified. The aim of this study was to evaluate by the experimental approach the competitive interaction between the two species, topmouth gudgeon and crucian carp, focusing on isotopic niche sizes and their overlap in syntopy. A four-month mesocosm experiment was performed to determine the isotopic niche of crucian carp and topmouth gudgeon living alone and in syntopy. Additionally, stable isotope data were collected at the sites where the two species co-occur to compare niche sizes and overlaps. Experimental data showed that the isotopic space of topmouth gudgeon responded more flexibly (reducing niche size at syntopy) than that of the crucian carp and confirmed a high isotopic niche overlap between the species. Field studies have shown that topmouth gudgeon has invaded the isotopic niche of the crucian carp, especially when another invasive species, the gibel carp, lived in the community (25% at 40% ellipse area and 50% at 95% ellipse area). When only the topmouth gudgeon and crucian carp were present in the field, the overlap was lower (3% and 48%, respectively) and directional overlap modelling showed that the crucian carp was more likely to invade the isotopic niche of topmouth gudgeon than vice versa. The data indicated that competition between crucian carp and topmouth gudgeon is likely, especially in syntopy with other invasive species. This study shows that the feeding plasticity of topmouth gudgeon likely facilitates its establishment outside its native range and, due to high isotopic niche overlap, threatens native fish with similar feeding ecology with competitive displacement.

Differential proliferation regulates multi-tissue morphogenesis during embryonic axial extension: integrating viscous modeling and experimental approaches.

A major challenge in biology is to understand how mechanical interactions and cellular behavior affect the shapes of tissues and embryo morphology. The extension of the neural tube and paraxial mesoderm, which form the spinal cord and musculoskeletal system, respectively, results in the elongated shape of the vertebrate embryonic body. Despite our understanding of how each of these tissues elongates independently of the others, the morphogenetic consequences of their simultaneous growth and mechanical interactions are still unclear. Our study investigates how differential growth, tissue biophysical properties and mechanical interactions affect embryonic morphogenesis during axial extension using a 2D multi-tissue continuum-based mathematical model. Our model captures the dynamics observed in vivo by time-lapse imaging of bird embryos, and reveals the underestimated influence of differential tissue proliferation rates. We confirmed this prediction in quail embryos by showing that decreasing the rate of cell proliferation in the paraxial mesoderm affects long-term tissue dynamics, and shaping of both the paraxial mesoderm and the neighboring neural tube. Overall, our work provides a new theoretical platform upon which to consider the long-term consequences of tissue differential growth and mechanical interactions on morphogenesis.

Relationship between mechanical behavior and process factors in friction stir welding aluminum alloys

The friction stir welding (FSW) procedure is the main topic of this research study among the various welding techniques. The study focuses on the interaction between the mechanical properties of 3003 aluminum alloy and the process parameters (rotation speed, welding speed, and dwell time) in the form of rolled plates of 2 mm thickness, end-to-end and welded at 90° and 45°. The welds were made by varying the speed of rotation (1000, 2000 rpm) and setting the tool feed at 500 mm/min. This experimental approach is also based on varying the tilt of the welding tool from 0° to 2°. It has been shown that the studied parameters play an important role in the characterization and optimization of the above mentioned weld joints. Therefore, and based on the results obtained, the use of the 90° joint remains the best in terms of strength.

Integration of equine assisted services and social-emotional learning: A preliminary evaluation of a life skills training program for at-risk youth

Abstract This study was a preliminary evaluation of a life skills training program conducted by the Equine Partnership Program (EPP) integrating Equine Assisted Services (EAS) with social emotional learning (SEL) competencies. SEL programs aim to foster character development, curb maladaptive behaviors, and enhance social skills in at-risk youth by promoting positive relationships and emotional regulation. Many youth still require more targeted or alternative approaches to effectively nurture these skills. EAS has the potential to enhance SEL through its experiential learning approach and use of horses as a bridge, which is believed to be effective because of the horse’s innate ability to mirror and respond to human emotion. The purpose of this study was to assess the impact of EAS on the social–emotional development of at-risk youth as measured by the Depression, Anxiety, Pain, and Stress (DAPS) visual analog tool. It was hypothesized that participants would demonstrate measurable improvements in emotional regulation post intervention. Data were collected before and after the EAS experience from 33 participants. Participants were asked to rate their levels of DAPS ranging from a high of 10 to a low of 1. Using t-test comparisons, statistically significant decreases in scores were found in depression (M = −0.67, p = 0.02), and anxiety (M = −1.18, p < 0.001). Emotional pain and stress also showed decreases, though not statistically significant. This intervention shows promise to serve as an effective modality in addressing resilience and pro-social skills necessary for improved outcomes in behavioral and emotional development, thereby improving future academic, career, and overall life success, including improved social relationships.

A STATISTICAL FRAMEWORK FOR THE MODELING OF THE CONTINUOUS PROGRESSION OF ALZHEIMER'S DISEASE.

Throughout an organism's life, a multitude of biological systems transition through complex biophysical processes. These processes serve as indicators of the underlying biological states. Inferring these latent unobserved states is a key problem in modern biology and neuroscience. Unfortunately, in many experimental setups we can at best obtain snapshots of the system at different times for different individuals, and one major challenge is the one of reconciling those measurements. This formalism is particularly relevant in the study of Alzheimer's Disease (AD) progression, in which we observe in brain donors the aggregation of pathological proteins but the underlying disease state is unknown. The progression of AD can be modeled by assigning a latent score - termed pseudotime - to each pathological state, creating a pseudotemporal ordering of donors based on their pathological burden. This paper proposes a hierarchical Bayesian framework to model AD progression using detailed quantification of multiple AD pathological proteins from the Seattle AD Brain Cell Atlas consortium (SEA-AD). Inspired by biophysical models, we model pathological burden as an exponential process. Theoretical properties of the model are studied, by using linearization to reveal convergence and identifiability properties. We provide Markov chain Monte Carlo estimation algorithms, and show the effectiveness of our approach with multiple simulation studies across data conditions. Applying the methodology to SEA-AD brain data, we infer pseudotime for each donor and order them by pathological burden. Finally, we analyze the information within each pathological feature and utilize it to refine the model by focusing on the most informative pathologies. This lays the groundwork for suggesting future experimental design approaches.

Engineering an inducible leukemia-associated fusion protein enables large-scale ex vivo production of functional human phagocytes

Ex vivo expansion of human CD34+ hematopoietic stem and progenitor cells remains a challenge due to rapid differentiation after detachment from the bone marrow niche. In this study, we assessed the capacity of an inducible fusion protein to enable sustained ex vivo proliferation of hematopoietic precursors and their capacity to differentiate into functional phagocytes. We fused the coding sequences of an FK506-Binding Protein 12 (FKBP12)-derived destabilization domain (DD) to the myeloid/lymphoid lineage leukemia/eleven nineteen leukemia (MLL-ENL) fusion gene to generate the fusion protein DD-MLL-ENL and retrovirally expressed the protein switch in human CD34+ progenitors. Using Shield1, a chemical inhibitor of DD fusion protein degradation, we established large-scale and long-term expansion of late monocytic precursors. Upon Shield1 removal, the cells lost self-renewal capacity and spontaneously differentiated, even after 2.5 y of continuous ex vivo expansion. In the absence of Shield1, stimulation with IFN-γ, LPS, and GM-CSF triggered terminal differentiation. Gene expression analysis of the obtained phagocytes revealed marked similarity with naïve monocytes. In functional assays, the novel phagocytes migrated toward CCL2, attached to VCAM-1 under shear stress, produced reactive oxygen species, and engulfed bacterial particles, cellular particles, and apoptotic cells. Finally, we demonstrated Fcγ receptor recognition and phagocytosis of opsonized lymphoma cells in an antibody-dependent manner. Overall, we have established an engineered protein that, as a single factor, is useful for large-scale ex vivo production of human phagocytes. Such adjustable proteins have the potential to be applied as molecular tools to produce functional immune cells for experimental cell-based approaches.

From whole bodies to single cells: A guide to transcriptomic approaches for ecology and evolutionary biology

AbstractRNA sequencing (RNAseq) methodology has experienced a burst of technological developments in the last decade, which has opened up opportunities for studying the mechanisms of adaptation to environmental factors at both the organismal and cellular level. Selecting the most suitable experimental approach for specific research questions and model systems can, however, be a challenge and researchers in ecology and evolution are commonly faced with the choice of whether to study gene expression variation in whole bodies, specific tissues, and/or single cells. A wide range of sometimes polarised opinions exists over which approach is best. Here, we highlight the advantages and disadvantages of each of these approaches to provide a guide to help researchers make informed decisions and maximise the power of their study. Using illustrative examples of various ecological and evolutionary research questions, we guide the readers through the different RNAseq approaches and help them identify the most suitable design for their own projects.

Effectiveness of a professional development program based on the instructional design framework for AI literacy in developing AI literacy skills among pre-service teachers

This study assessed AI literacy among a group of preservice-teachers, and investigated the effectiveness of a suggested professional development program based on the Instructional Design Framework for AI literacy in developing pre-service teachers AI literacy skills. A quasi experimental approach with post-test pretest design was used for data collection. Thirty-seven undergraduate students from Palestine Technical University Kadoorie and Hebron University participated in this study. An AI literacy scale was developed, validated, and used for data collection. The results showed that the professional development program was effective in developing the AI literacy skills among the participant preservice teachers. Gender and specialty area of the participants did not significantly affect preservice teachers AI literacy skills after participating in the professional development program. Given the research results, it is recommended that teachers should be supported to integrate AI in classroom activities. AI technological tools should be included in pre-service and in-service training programs. Further AI tools combined with other professional development strategies should be researched among teachers from different subjects to enable generalizing the finding of this research and increasing it is validity.

RISING STARS: Evidence for established and emerging forms of β-cell death.

β-Cell death contributes to β-cell loss and insulin insufficiency in type 1 diabetes (T1D), and this β-cell demise has been attributed to apoptosis and necrosis. Apoptosis has been viewed as the lone form of programmed β-cell death, and evidence indicates that β-cells also undergo necrosis, regarded as an unregulated or accidental form of cell demise. More recently, studies in non-islet cell types have identified and characterized novel forms of cell death that are biochemically and morphologically distinct from apoptosis and necrosis. Several of these mechanisms of cell death have been categorized as forms of regulated necrosis and linked to inflammation and disease pathogenesis. In this review, we revisit discoveries of β-cell death in humans with diabetes and describe studies characterizing β-cell apoptosis and necrosis. We explore literature on mechanisms of regulated necrosis including necroptosis, ferroptosis and pyroptosis, review emerging literature on the significance of these mechanisms in β-cells, and discuss experimental approaches to differentiate between various mechanisms of β-cell death. Our review of the literature leads us to conclude that more detailed experimental characterization of the mechanisms of β-cell death is warranted, along with studies to better understand the impact of various forms of β-cell demise on islet inflammation and β-cell autoimmunity in pathophysiologically relevant models. Such studies will provide insight into the mechanisms of β-cell loss in T1D and may shed light on new therapeutic approaches to protect β-cells in this disease.

Individual quality overwrites carry-over effects across the annual cycle of a long-distance migrant.

In seasonal environments, the fitness of animals depends upon the successful integration of life-history stages throughout their annual cycle. Failing to do so can lead to negative carry-over effects where individuals are transitioning into the next season in different states, consequently affecting their future performance. However, carry-over effects can be masked by individual quality when individuals vary in their efficiency at acquiring resources year after year (i.e. 'quality'), leading to cross-seasonal consistency in individual performance. Here we investigated the relative importance of carry-over effects and individual quality in determining cross-seasonal interactions and consequences for breeding success over the full annual cycle of a migratory seabird (black-legged kittiwake Rissa tridactyla). We monitored the reproduction and annual movement of kittiwakes over 13 years using geolocators to estimate their breeding success, distribution and winter energy expenditure. We combined this with an experimental approach (clutch removal experiment, 2 years) to manipulate the reproductive effort irrespective of individual quality. Piecewise path analyses showed that successful breeders reproduced earlier and were more likely to breed successfully again the following year. This positive interaction among consecutive breeding stages disappeared after controlling for individual quality, suggesting that quality was dominant in determining seasonal interactions. Moreover, controlling experimentally for individual quality revealed underlying carry-over effects that were otherwise masked by quality, with breeding costs paid in higher energy expenditure and delayed onset of reproduction. We highlight the need to combine an experimental approach along with long-term data while assessing apparent carry-over effects in wild animals, and their potential impact on fitness and population demography.

Green Method Comparison and Optimization of Anthocyanin Recovery from "Sangiovese" Grape Pomace: A Critical Evaluation of the Design of Experiments Approach.

Grape pomace is the main by-product obtained from wine production that is still enriched in bioactive compounds. Within a framework of waste/by-product reuse through a sustainable approach, various green methods were utilized in this work to recover anthocyanins from the pomace resulting from "Sangiovese" grape vinification. Ultrasound- and Microwave-Assisted Extractions (UAE and MAE) were coupled with the use of green solvents, such as acidified water, an ethanol/water mixture, and Natural Deep Eutectic Solvents (NaDES), and their efficacy was compared with that of a conventional method based on a methanol/acidified water mixture. The Total Anthocyanin Index ranged from 36.9 to 75.2 mg/g DW for UAE, and from 54.4 to 99.6 mg/g DW for MAE, while resulting in 47.1 mg/g DW for conventional extraction. A Design of Experiments (DoE) approach was applied to MAE, the most efficient technique. Temperature, time, and the solid-to-liquid ratio were set as X variables, while malvidin-3-O-glucoside content and antioxidant activity were used as response variables, measured by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The correlation between temperature and time and the antioxidant activity of the extract was positive, while it was found to be negative when considering malvidin-3-O-glucoside concentration as a response variable. Thus, the optimal conditions in temperature, time and solid-to-liquid ratio were different depending on the chosen variable. The results underline the importance of selecting an accurate response when using the response surface methodology approach.

Motivation and Play Activities for Children's Basic Movements

Delays in basic movements during early childhood represent a challenge that must be addressed by sports professionals. This research aims to describe how play and motivation relate to children's basic movements, implementing an experimental model approach. The sample participants were children who were still in primary school, with an average age of 11.09 ± 0.73 years. Data were collected using a training motivation questionnaire and the Scott motor ability test, which consisted of basketball throw, 4-second run, wall pass, and long jump. Data were analyzed using a two-way factorial ANOVA test. The research results show that the kasiti ball training model is more effective than fortification training. Furthermore, there is an interaction between the form of training and motivation: the basic movements performed in the kasiti ball training are superior to the strengthening ones for children with high training motivation, while the baseball training is better than the of strength for those with low motivation. This finding is important as it informs children's basic movement skills through specific exercises. Keywords: Children, basic movements, motivation, forms of play.

Prominent antidiabetic and anticancer investigation of Scrophularia deserti extract: Integration of experimental and computational approaches

Diabetes mellitus (DM) is a group of syndromes and complications characterized by hyperglycemia, metabolic disorders in lipids, carbohydrates, and proteins. It causes an increased risk of vascular complications. A methanolic extract of Scrophularia deserti was found to reduce blood glucose in basal conditions and after heavy glucose load in normal rats. S. deserti extract lowered the hyperglycemia in streptozotocin-induced diabetic rats (33.33 % at 200 mg/kg, 41.43 % at 400 mg/kg, 2-, and 4-hours following administration when compared to standard glibenclamide (52.05 %). Furthermore, the in vitro activity of the extract was screened against two major diabetes targets; human α-glucosidase and human α-amylase, and showed 88–87 % inhibition effect compared to reference drugs. In addition, the Scrophularia deserti extract was assayed for detection of its antioxidant assay as a free radical scavenger. In the cytotoxicity experiment, the extract exhibited a good cytotoxic selectivity to cancer over normal cell lines. Herin, we categorized all active components existing in the plant extract as defined by LC-MS tool for further computational studies. Certain molecular modeling studies have been done including docking experiments and molecular dynamic simulations for the most predicted potent active metabolites; Caffeic acid 3-glucoside and 1-O-feruloyl-β-d-glucose. The results revealed good binding and stability behavior within the active sites of both targets.

Dietary and homeostatic controls of Zn isotopes in rats: a controlled feeding experiment and modeling approach.

The stable isotope composition of zinc (δ66Zn), which is an essential trace metal for many biological processes in vertebrates, is increasingly used in ecological, archeological, and paleontological studies to assess diet and trophic level discrimination among vertebrates. However, the limited understanding of dietary controls and isotopic fractionation processes on Zn isotope variability in animal tissues and biofluids limits precise dietary reconstructions. The current study systematically investigates the dietary effects on Zn isotope composition in consumers using a combined controlled feeding experiment and box-modeling approach. For this purpose, 21 rats were fed one of seven distinct animal- and plant-based diets and a total of 148 samples including soft and hard tissue, biofluid, and excreta samples of these individuals were measured for δ66Zn. Relatively constant Zn isotope fractionation is observed across the different dietary groups for each tissue type, implying that diet is the main factor controlling consumer tissue δ66Zn values, independent of diet composition. Furthermore, a systematic δ66Zn diet-enamel fractionation is reported for the first time, enabling diet reconstruction based on δ66Zn values from tooth enamel. In addition, we investigated the dynamics of Zn isotope variability in the body using a box-modeling approach, providing a model of Zn isotope homeostasis and inferring residence times, while also further supporting the hypothesis that δ66Zn values of vertebrate tissues are primarily determined by that of the diet. Altogether this provides a solid foundation for refined (paleo)dietary reconstruction using Zn isotopes of vertebrate tissues.

Lost-wax casting: A widespread technique to produce copper alloy adornments in Atlantic Europe since the mid-second millennium BC

Lost-wax casting is a metalworking technique that has long been regarded as an innovation imported from the eastern Mediterranean and only widely used in Western Europe since the Late Bronze Age. We now have evidence that this technique was in fact largely in use from the Middle Bronze Age onwards for the production of copper alloy adornments in the Atlantic area. Along with palstaves, these objects are the most abundant in Atlantic hoards from the 15th to the 13th centuries BC. Thanks to detailed morphological and technological study, combined with an experimental approach and analysis of their elemental composition, we can propose new chaînes opératoires for the manufacture of massive annular bracelets with geometric decoration.

A review on modeling of graphene and associated nanostructures reinforced concrete

Abstract Concrete is the most popular construction material in infrastructure projects due to its numerous natural advantages. Nevertheless, concrete constructions frequently suffer from low tensile strength and poor durability performance which are always urgent tasks to be solved. The concrete reinforced by various nanomaterials, especially graphene and its associated nanostructures (GANS), shows excellent chemical and physical properties for engineering applications. The influence of GANS on cement composites is a multiscale behavior from the nanoscale to the macroscale, which requires a number of efforts to reveal via numerical and experimental approaches. To meet this need, this study provides a comprehensive overview of the numerical modeling for GANS reinforced concrete in various scales. The background and importance of the topic are addressed in this study, along with the review of its methodologies, findings, and applications. Moreover, the study critically summarizes the performance of GANS reinforced concrete, including its mechanical behavior, transport phenomena, and failure mechanism. Additionally, the primary challenges and future prospects in the research field are also discussed. By presenting an extensive overview, this review offers valuable insights for researchers and practitioners interested in numerical simulation to advance concrete science and engineering.

Development of optimal L-PBF process parameters using an accelerated discrete element simulation framework

Laser Powder Bed Fusion (L-PBF) has immense potential for the production of complex, lightweight, and high-performance components. The traditional optimization of process parameters is costly and time-intensive, due to reliance on experimental approaches. Current numerical analyses often model single-line scans, while it is necessary to model multiple fully scanned layers to optimize for bulk material quality. Here, we introduce a novel approach utilizing discrete element simulations with a ray tracing-modeled laser heat source. Our approach significantly reduces the cost and time consumption compared to conventional optimization methods. GPU acceleration enables efficient simulation of multiple layers, resulting in parameters optimized for bulk material. In a case study, parameters were optimized for AlSi10Mg in just 5 days, a process that would have taken over 8 months without GPU acceleration. Experimental validation affirms the quality of the optimized process parameters, achieving an optical density of 99.91%.Graphical Optimization using the accelerated simulation yielded an optimized parameter set within 5 days. This resulted in apart with an optical density of 99.91%.

The effect of lightweight geopolymer concrete containing air agent on building envelope performance and internal thermal comfort

Innovative building materials are the primary focus for researchers seeking to reduce energy consumption and advance global sustainability initiatives. This target could accomplished by addressing environmental issues and confronting the adverse impact of climate change. In this regard, this paper used a combined experimental and simulation approach to evaluate the effectiveness of lightweight geopolymer concrete (LWGC) incorporating industrial waste of aluminum powder (AP) and ferrosilicon waste powder (FWP) for reducing the energy consumption in the building envelop. Seven concrete mixes for LWGC were prepared using an air operator. Three mixtures contain 0.1 %, 0.2 %, and 0.3 % AP (FA), and three mixtures contain 0.1 %, 0.2 %, and 0.3 % FWP by weight of fly ash. The workability, unit volume weight, compressive strength, thermal conductivity, and microstructure analysis of the LWGC were examined. In addition, energy simulations were performed to evaluate their ability to reduce energy use in residential buildings. The inclusion of AP and FWP in LWGC resulted in a significant decrease in the density of the material, which amounts to 55 % for AP0.3 and 35 % for FWP0.3. The diameter of the LWGC pore structure increased as the Ca/Si ratio was reduced. The AP and FWP materials reduced energy consumption for building envelope elements such as external walls and roof insulation by 14.5 %, resulting in a 9.2 % overall reduction compared to the traditional concrete mix as a control sample.

Treatment Preferences for Acute Allergic Reactions: A Discrete Choice Experiment

Background: Timely treatment of acute allergic reactions (AARs) is important to minimize reaction severity. Corticosteroid tablets dissolved in water are commonly used in mainstay treatment. A new oral film that dissolves on the tongue provides a faster and less cumbersome alternative to tablets for corticosteroid administration during AARs. This study evaluated patients’ preferences for attributes related to administration mode of corticosteroids in AARs. Methods: A web-based survey was sent to a sample from the adult Swedish population (≥18 years) with experience of corticosteroid treatment for AAR. We assessed the willingness to pay (WTP) for attributes related to corticosteroid treatment by applying a discrete choice experiment (DCE) approach. DCE attributes were administration mode, time to symptom relief, and price. The WTP for each attribute was derived using the attribute’s coefficient in a logistic regression analysis. We specified a forced choice (FC) and an unforced choice (UC) model. In the FC model, the respondents chose between 2 hypothetical treatments and in the UC model, between any of 2 hypothetical treatments and their current treatment. Results: The final study population included 348 subjects, of which 80% were women. All the evaluated DCE attributes were significant predictors for the treatment choice (p<.001). In the FC model, the incremental WTP for an oral film compared with tablets was 409 Swedish kronor (SEK [≈€36.7]), with no other factors considered. In the UC model, the incremental WTP for the oral film compared with tablets was 574 SEK (≈€51.7). After considering the value of the respondents’ current treatment, the WTP for the oral film decreased to 336 SEK (≈€30.3). The total WTP was reduced by 17 SEK (≈€1.5) per minute of shorter time to symptom relief. Subgroup analyses showed that people with circulatory symptoms and experience of swallowing difficulties related to allergy medication had higher WTP for the oral film than the average respondent. Conclusion: The findings show a substantial economic benefit of the oral film vs tablets for patients with AARs in Sweden. This result remained also after compensation for the full value of the patients’ current treatment.