Expansion Capacity Research Articles

VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment

CD8+T cells within the tumor microenvironment (TME) are often functionally impaired, which limits their ability to mount effective anti-tumor responses. However, the molecular mechanisms behind this dysfunction remain incompletely understood. Here, we identified valosin-containing protein (VCP) as a key regulator of CD8+T cells suppression in hepatocellular carcinoma (HCC). Our findings reveal that VCP suppresses the activation, expansion, and cytotoxic capacity of CD8+T cells both in vitro and in vivo, significantly contributing to the immunosuppressive nature of the TME. Mechanistically, VCP stabilizes the expression of glycerol-3-phosphate dehydrogenase 1-like protein (GPD1L), leading to the accumulation of glycerol-3-phosphate (G3P), a downstream metabolite of GPD1L. The accumulated G3P diffuses into the TME and directly interacts with SRC-family tyrosine kinase LCK, a critical component of the T-cell receptor (TCR) signaling pathway in CD8+T cells. This interaction heightens the phosphorylation of Tyr505, a key inhibitory residue, ultimately reducing LCK activity and impairing downstream TCR signaling. Consequently, CD8+T cells lose their functional capacity, diminishing their ability to fight against HCC. Importantly, we demonstrated that targeting VCP in combination with anti-PD1 therapy significantly suppresses HCC tumor growth and restores the anti-tumor function of CD8+T cells, suggesting synergistic therapeutic potential. These findings highlight a previously unrecognized mechanism involving VCP and G3P in suppressing T-cell-mediated immunity in the TME, positioning VCP as a promising upstream target for enhancing immunotherapy in HCC.

Acquisition of innate B cell properties and generation of autoreactive IgA antibodies by follicular B cells during homeostatic proliferation

The physiologic process of homeostatic proliferation serves to restore the pool of peripheral lymphocytes in response to lymphopenia. However, functional changes in B cell responses during homeostatic proliferation are still only insufficiently characterized. Mature peripheral B cells consist of functionally distinct B cell subsets, such as adaptive follicular B cells (FoBs) and the innate B cell subsets, marginal zone B cells (MZBs) and B1a B cells. During homeostatic proliferation, B cells undergo antigen-independent clonal expansion and differentiation into antibody-producing plasma cells (PCs). However, it is still largely unknown which B cell lineages are involved in the formation of antibodies in response to lymphopenia and what functional properties these antibodies have. Employing adoptive transfer of different mature B cell subsets into lymphopenic Rag2-/- hosts, we here show that not only innate B cells – MZBs and B1a cells – but also adaptive FoBs were capable to differentiate into PCs and to produce IgM and class-switched IgA serum antibodies in a T cell-independent fashion during homeostatic proliferation. In light of the poor reactivity of FoBs to innate stimulation in vitro, the observed high expansion capacity of FoBs, their sustained repopulation of lymphoid and intestinal organs and their particularly prominent ability to induce class-switched auto-/polyreactive IgA antibodies in this antigen- and T cell-independent system was rather unexpected. These properties, which are more typical for innate B cells, were associated with a striking plasticity of FoBs that transdifferentiate into MZB-like cells under lymphopenic conditions. Together, our study indicates that the reconstitution of antibodies in response to lymphopenia-induced homeostatic B cell proliferation is mainly elicited by innate MZB-like B cell responses via antigen- and T cell-independent pathways resulting in the selection of autoreactive IgA antibodies. In addition, our data point to the pathogenic potential of the conversion of conventional adaptive B cells, which are the most common population of mature B cells, into innate-like B cells and the production of autoreactive IgA antibodies during homeostatic proliferation. This process could also manifest as clinical complication of therapy-induced lymphopenia in the context of transplantation and cancer in human patients.

When Broad Learning System Meets Label Noise Learning: A Reweighting Learning Framework.

Broad learning system (BLS) is a novel neural network with efficient learning and expansion capacity, but it is sensitive to noise. Accordingly, the existing robust broad models try to suppress noise by assigning each sample an appropriate scalar weight to tune down the contribution of noisy samples in network training. However, they disregard the useful information of the noncorrupted elements hidden in the noisy samples, leading to unsatisfactory performance. To this end, a novel BLS with adaptive reweighting (BLS-AR) strategy is proposed in this article for the classification of data with label noise. Different from the previous works, the BLS-AR learns for each sample a weight vector rather than a scalar weight to indicate the noise degree of each element in the sample, which extends the reweighting strategy from sample level to element level. This enables the proposed network to precisely identify noisy elements and thus highlight the contribution of informative ones to train a more accurate representation model. Thanks to the separability of the model, the proposed network can be divided into several subnetworks, each of which can be trained efficiently. In addition, three corresponding incremental learning algorithms of the BLS-AR are developed for adding new samples or expanding the network. Substantial experiments are conducted to explicate the effectiveness and robustness of the proposed BLS-AR model.

HTXRD based lattice thermal expansion and specific heat capacity modelling of C15-Fe2Zr Laves phase

Crystallographic phase pure C15-Fe2Zr was obtained through arc melting technique. X-ray Diffraction (XRD), scanning electron microscope (FESEM) along with energy-dispersive X-ray analysis (EDS) and differential scanning calorimetry (DSC)were employed to study the single phase in Fe-33.3at.% Zr alloy. Rietveld refinement of the high-temperature XRD patterns (from 298K-873 K) revealed the average linear and volumetric coefficients of thermal expansion of the alloys as αai= 0.3643 × 10−5 K−1 and αVi = 1.0916 × 10−5 K−1, respectively. The experimental heat capacity and enthalpy increment of the C15-Fe2Zr phase from 310K to 890K was modelled employing Quasi-harmonic Debye Grüneisen theoretical modelling by identifying the vibrational, electronic, anharmonic and magnetic contributions. Curie temperature(~625K) was determined from the magnetic contribution of the heat capacity.

Rapidly Manufactured CAR-T with Conserved Cell Stemness and Distinctive Cytokine-Secreting Profile Shows Improved Anti-Tumor Efficacy.

Background: The emergence of chimeric antigen receptor T-cell (CAR-T) immunotherapy holds great promise in treating hematologic malignancies. While advancements in CAR design have enhanced therapeutic efficacy, the time-consuming manufacturing process has not been improved in the commercial production of CAR-T cells. In this study, we developed a "DASH CAR-T" process to manufacture CAR-T cells in 72 h and found the excelling anti-tumor efficacy of DASH CAR-T cells over conventionally manufactured CAR-T cells. Methods: Four different CAR-T manufacturing processes were first proposed and examined by flow cytometry in regard to cell viability, T-cell purity and activation, CAR expression, and cell apoptosis. The selected two processes, 48H DASH CAR-T and 72H DASH CAR-T, were applied to the subsequent functional assessments, including T-cell differentiation, antigen-dependent cytotoxicity and expansion, cytokines secretion profile, and in vivo anti-tumor efficacy. Results: We demonstrated that rapidly manufactured CAR-T cells generated within 48-72 h was feasible and exhibited increased naïve and memory T-cell ratios, a distinctive secretory profile, superior expansion capacity, and enhanced in vitro and in vivo anti-tumor activity compared to conventionally manufactured CAR-T cells. Conclusions: Our findings suggest that "DASH CAR-T" process is a valuable platform in reducing CAR-T manufacturing time and producing high-efficacy CAR-T cells for future clinical application.

Novel flexible and active expanded-starch films enriched with Agrifood waste via microwave irradiation

Expanded materials possess valuable properties for food packaging, including thermal insulation and lightweight. However, developing sustainable alternatives with the potential to replace lightweight petroleum-based plastics is highly challenging. Starch biopolymer is a promising sustainable candidate due to its compostable character, great foamability and processability. Moreover, its chemical characteristics allow its combination with agrifood wastes to promote the circular economy and provide active properties. Most of the starch foams reported with low density are processed via extrusion and they are notably brittle and rigid, restricting their application. In this work, different expanded starch biocomposites, without or enriched with 40 wt.% orange peel (OP), were fabricated using 30 s of microwave (MW) irradiation, acquiring different properties depending on the main blowing agent used (i.e., water, sodium bicarbonate or expandable microspheres). These samples were fully characterized in terms of morphology, density, expansion degree, water uptake and solubility, mechanical properties, migration and antioxidant properties. Remarkably, the final expanded materials reached a considerably low density of 0.3 g/cm3 and they exhibited exceptional flexibility, overcoming the important drawback of starch foams. Finally, the benefits of adding 40 wt.% of agrifood waste were not only environmental, but OP increased the expansion capacity and gave outstanding antioxidant properties.

Thermophysical modeling of niobium alloys informs materials selection and design for high-temperature applications

There is renewed interest in refractory alloys that possess higher service temperatures than incumbent Ni-based superalloys (⪆1100 °C). Thermophysical property data for six Nb-alloys are gathered from the literature and reviewed, and new data are provided for two Hf-containing Nb-alloys; elastic modulus, thermal expansion, thermal conductivity, and heat capacity are presented for C103, and new thermal conductivity data are provided for a higher strength alloy, WC-3009. Comparisons with Ni-superalloys and other refractory-metal based alloys provide context. Physics-based models are provided that describe the temperature dependencies of the Young’s modulus, coefficient of thermal expansion and density, and thermal conductivity; such that fair comparisons can be made across alloys for any given condition. The results suggest a need for improved understanding of the temperature dependence of the elastic modulus. A performance index is introduced for making informed materials selection decisions in the context of lightweight, panel-shaped applications subjected to sharp thermal transients or steep thermal gradients, and the significant strain rate sensitivity of Nb-alloys is highlighted. Ultimately, the relative value of current commercial alloy, C103, as well as the promise of specific Nb-W-Zr alloys are highlighted.

Effect of electron beam irradiation treatment on microstructure, physicochemical properties, and bioactive content of areca nut.

Electron beam irradiation treatment is a novel technology that uses low-dose ionizing radiation for the treatment of crops or food to enhance their quality. This study investigated the effects of electron beam irradiation on the microstructure, physicochemical properties, and bioactive compounds of areca nuts. As the irradiation dose increased, the cellulose, hemicellulose, and lignin content in the areca nuts decreased significantly, whereas the polysaccharide and pectin content increased gradually. The hardness, chewiness, and adhesiveness of areca nuts reached their lowest values when the irradiation dose was within the range of 6-9 kGy, indicating that irradiation effectively reduced the hardness of the areca nut fibers. The decrease in crystallinity led to the formation of loose structures in the fibers upon irradiation, thereby improving their water retention, expansion, and oil-holding capacity, which are beneficial for the subsequent processing of areca nut-based chewable products. The water- and oil-holding capacities of the areca nuts peaked when the irradiation dose was within the 6-9 kGy range. Electron irradiation also affected the content of active substances in the areca nuts, particularly alkaloids, flavonoids, and polyphenols, showing an overall trend of initial increase followed by subsequent decrease. Electron irradiation was not only effective in softening the fibers but it also impacted the overall quality of the areca nuts significantly. The results provide valuable reference data for improving the quality of areca nuts through electron beam irradiation technology. © 2024 Society of Chemical Industry.

Design and application of graphite film with high thermal conductivity in SAR antenna

Abstract The rapid development of SAR antenna in the remote sensing field has put forward the test of antenna thermal design. In this paper, according to the load of a certain type of extravehicular SAR antenna, a reasonable thermal design is carried out for its high heat consumption and external heat flux. A high thermal conductivity graphite film is used for heat expansion. The test shows that the mounting plate heat expansion capacity difference is 28°C with and without graphene film, with 40W heat consumption applied in 180mm transmission distance. The simulation analysis and thermal balance test are carried out. The results show that the high thermal conductivity graphite film can rapidly extend the heat of the single unit of the SAR antenna to the whole antenna, and make the antenna meet the temperature requirement in orbit, which proves the rationality of thermal design. This paper is useful for the thermal control design of a satellite-borne SAR antenna.

Influences of superfine-grinding and mix enzymolysis alone or combined with hydroxypropylation or acetylation on the hypolipidemic and hypoglycemic properties of coconut endosperm residue fiber.

Coconut endosperm residue is an abundant and low-cost resource of dietary fiber, but the low soluble fiber content limits its functional properties and applications in the food industry. To improve the hypolipidemic and hypoglycemic properties, coconut endosperm residue fiber (CERF) was modified by superfine-grinding and mix enzymatic hydrolysis alone, or combined with acetylation or hydroxypropylation. The effects of these modifications on the structure and functional properties were studied using scanning electron microscopy, Fourier-transformed infrared spectroscopy, and in vitro tests. After these modifications, the microstructure of CERF became more porous, and its soluble fiber content, surface area, water adsorption, and expansion capacities were all improved (p < 0.05). Moreover, superfine-grinding and mix enzymolysis combined with acetylation treated CERF showed the highest surface hydrophobicity (48.96) and cholesterol and cholate adsorption abilities (33.72 and 42.04mg∙g‒1). Superfine-grinding-, mix enzymolysis-, and hydroxypropylation-treated CERF exhibited the highest viscosity (17.84 cP), glucose adsorption capacity (29.61µmol∙g‒1), and glucose diffusion inhibition activity (73.96%), and water-expansion ability (8.60mL∙g‒1). Additionally, superfine-grinding and mix enzymatic hydrolyzed CERF had the highest α-amylase inhibiting activity (42.76%). Therefore, superfine-grinding and mix enzymolysis alone or combined with hydroxypropylation were better choices to improve hypoglycemic properties of CERF; meanwhile, superfine-grinding and mix enzymolysis combined with acetylation can effectively improve its hypolipidemic properties. PRACTICAL APPLICATION: This study offered three composite modification methods to improve the soluble fiber content and in vitro hypolipidemic and hypoglycemic properties of coconut endosperm residue fiber. These modification methods were practicable and low-cost. Moreover, it provides good choices to improve the functional properties and applications of other dietary fibers in the food industry.

Utilization of thermoplastic resin as foamed asphalt modifier in cold recycled mixtures

The utilization of foamed virgin asphalt in cold recycled mixtures results in insufficient bonding strength between aggregates, leading to rapid deterioration to the recycled asphalt pavement. This ultimately causes inefficient clean production and increased carbon emissions. The foaming performance of thermoplastic resin modified asphalt (TRA) under different foaming conditions was analyzed. Micro-scale chemical and physical tests were utilized to discover the foaming mechanism of TRA. The road performance of cold recycled mixtures with foamed TRA was evaluated through splitting, rutting and fatigue tests. The results showed that the increase in the TRA polar functional group index led to an increase in its surface tension, thus limiting its expansion capacity. However, the increase in the specific heat capacity of TRA indicated that it preserves a low viscosity level essential for its foaming efficacy post-ejection from the foaming machine, thereby enhancing its half-life. Furthermore, the dynamic viscosity of the TRA with an 8% thermoplastic resin dosage exceeded 40000 Pa s at 60 °C, meeting the specification requirements for high viscosity asphalt, while exhibiting a notably lower viscosity of only 120 mPa s at the foaming temperature of 170 °C. The observed low viscosity of the asphalt is anticipated to favorably enhance the formation of a stable foam structure. The mechanical properties and overall road performance of the cold recycled mixture with foamed TRA were significantly enhanced, especially in terms of moisture damage resistance and low-temperature crack resistance. The purpose of this paper is to serve as a valuable reference for investigating the mechanism of modified asphalt foaming and to enhance the efficiency of cold recycled pavement regeneration processes.

Developmental characteristics of vertical natural fracture in low-permeability oil sandstones and its influence on hydraulic fracture propagation

Vertical natural fractures (NFs) are prevalent in low-permeability sandstone reservoirs. Presently, the impact of NFs on the extension of hydraulic fractures (HFs) remains partially unveiled, which restricts the scientific development of strategies for low-permeability, fractured oil sandstones. In this study, taking the oil sandstone of the He-3 Member, Hetaoyuan Formation, southeastern Biyang Depression as an example, we conducted a comprehensive investigation into the factors influencing vertical fracture development and the interaction between natural and hydraulic fractures. The cohesive unit simulations indicate that geostress is the principal factor influencing HF expansion, more so than NFs, with this influence intensifying as natural fracture density increases. As natural fracture density grows, the potential for two sets of conjugate natural fractures to form short HFs arises, which are limited in expansion scope, suggesting a need to reduce well spacing accordingly. Conversely, areas with a single set of NFs are more prone to developing longer HFs, warranting an increase in well spacing to avoid water channeling. High natural fracture densities may constrain the effectiveness of HFs. In fractured reservoirs with a 10 MPa horizontal stress difference, the length of HFs is 1.52 times that of HFs with 0 MPa and 5 MPa differences. However, the hydraulic fracture effectiveness index (FE) of the latter is 1.74 times higher than the former. For fractured reservoirs, the expansion capacity of HF length within a 5 MPa horizontal stress difference remains relatively stable; beyond this threshold, the expansion capacity increases with the growing horizontal stress difference, and the fracturing effect eventually deteriorates. Furthermore, as the strength of NFs escalates, the length and modified area of HFs initially decrease significantly before stabilizing. The complexity and FE value of HFs formed under strong natural fracture conditions are heightened, indicating a more effective fracturing outcome.

Pressure-Dependent Thermal and Mechanical Behaviour of a Molecular Crystal of Bromine.

This study investigates the pressure-dependent thermal and mechanical properties of solid bromine through density functional theory (DFT) calculations used in conjunction with the quasi-harmonic approximation (QHA). At ambient pressure, bromine crystallizes as a molecular crystal of Cmca symmetry. Previous studies have indicated that upon compression, this polymorph should undergo a bandgap closure at 80 GPa followed by a phase transition to a nonmolecular phase at 90 GPa. By employing QHA, we model the lattice vibrations and calculate the free energy, thermal expansion, and specific heat capacities of solid molecular bromine over a temperature range from 0 to 1000 K and pressures up to 90 GPa. Furthermore, mechanical properties such as bulk modulus and elastic constants are also analyzed. The results reveal the significant impact that pressure has on the thermal properties, mechanical stability, and dynamical stability of a molecular crystal. These findings contribute to a deeper understanding of such systems under extreme conditions, potentially guiding future experimental and theoretical investigations.

A combinatorial culture strategy to develop pseudomyxoma peritonei organoid models.

Few preclinical models of pseudomyxoma peritonei (PMP) have been developed, probably due to the tumor's low incidence and its peculiar characteristics of slow growth. Therefore, there is a need to develop more refined PMP models that better reflect its characteristics. The aim of the study is to develop a culture strategy to generate organoid models derived from PMP patient samples. We followed a strategy based on combinatorial culture conditions that include the different factors essential for PMP growth and that mimic the microenvironment present in the patients. We cultured PMP samples in the presence of the various factors produced by the niche environment of PMP. We obtained 12 PMP organoid models, each of which grows under specific culture conditions. PMP-derived organoids show long-term expansion capacity and reproduce the genetic landscape and histological phenotype of the tumor of origin. The organoids we developed faithfully reproduce the key features of PMP disease and will allow us to understand the biology of PMP. With them, we will be able to identify key regulatory networks that support PMP progression, providing a platform for multilevel preclinical testing, identify novel diagnostic biomarkers, and generate novel targets for patient treatments.

Antigen-independent activation is critical for the durable antitumor effect of GUCY2C-targeted CAR-T cells

BackgroundChimeric antigen receptor (CAR)-T cells face many obstacles in solid tumor therapy, including heterogeneous antigen expression and inefficient T cell persistence. Guanylyl cyclase C (GUCY2C) has been identified as a...

Environmental sustainability and waste conversion of Prosopis juliflora fibre-reinforced ZnO nanofiller particulates PLA composite- mechanical and thermal analysis

The presence of large quantities of Prosopis juliflora (PJ) fibers in natural habitats presents substantial threats to the environment and economies of numerous developing countries. Utilizing natural fibers in polymer composites can effectively enhance their characteristics. The primary objective of this study is to create a composite material by combining Prosopis Juliflora (PJ) fiber with a polylactic matrix that has been combined with zinc oxide nanofillers. The fabrication process will involve the hand layup technique. In order to have a comprehensive understanding of the mechanical characteristics, thermal behavior, and thermal stability of the PJ composite, it is necessary to undertake additional investigations. The results showed that the inclusion of zinc oxide filler enhanced the tensile strength (67.29 MPa), flexural strength (64.27 MPa), compressive strength (56.79 MPa), and impact energy (34 J) in sample S5. Additionally, the thermal properties, including thermal conductivity, thermal expansion, and short-term heat resistant capacity, were also improved by the addition of zinc oxide filler in sample S5. The deterioration temperature of the PJ composite was determined to be between 312 and 342 °C using thermogravimetric analysis. The failure mode of the PJ composite was investigated using scanning electron microscopy.

Effectiveness of Intercostal Stretch Technique on Pulmonary Conditions: A Narrative Review

Background: The Intercostal Stretch Technique is one of the physiotherapy techniques that is used to solve different respiratory problems. It is used for increasing chest expansion and diaphragm excursion improvement and also intra-thoracic lung volume. Aim: The study aims to evaluate the effectiveness of the intercostal stretch technique. Methodology: A narrative review. In this study, an RCT study was used to review the intervention. Results: Several articles are used to discuss the effectiveness of the intercostal stretch technique. In stable COPD, diaphragmatic breathing and the intercostal stretch technique both work equally well to improve chest expansion and functional capacity while lowering dyspnea. Research has shown that IC stretch outperforms the anterior basal lift technique in lowering heart and respiratory rates while increasing oxygen saturation. According to this study, IC stretching in conjunction with breathing control may improve dynamic lung parameters, particularly FEV1/FVC%, more than breathing control alone. Patients with COPD have an increased functional exercise capacity and reduced dyspnea when aerobic training and respiratory muscle stretching are combined. Conclusion: Different articles demonstrated that IC stretch improved lung function, expired tidal volume, decreased dyspnea, and increased chest expansion.

Adipose-Derived Stem Cell Therapy in Spinal Cord Injury.

Spinal cord injury (SCI) is a serious condition accompanied by severe adverse events that affect several aspects of the patient's life, such as motor, sensory, and functional impairment. Despite its severe consequences, definitive treatment for these injuries is still missing. Therefore, researchers have focused on developing treatment strategies aimed at ensuring full recovery post-SCI. Accordingly, attention has been drawn toward cellular therapy using mesenchymal stem cells. Considering their wide availability, decreased immunogenicity, wide expansion capacity, and impressive effectiveness in many therapeutic approaches, adipose-derived stem cell (ADSC) injections in SCI cases have been investigated and showed promising results. In this review, SCI pathophysiology and ADSC transplantation benefits are discussed independently, together with SCI animal models and adipose stem cell preparation and application techniques. The mechanisms of healing in an SCI post-ADSC injection, the outcomes of this therapeutic approach, and current clinical trials are also deliberated, in addition to the challenges and future perspectives, aiming to encourage further research in this field.

Stimuli-Responsive Phosphate Hydrogel: A Study on Swelling Behavior, Mechanical Properties, and Application in Expansion Microscopy.

Phosphorus-based stimuli-responsive hydrogels have potential in a wide range of applications due to their ionizable phosphorus groups, biocompatibility, and tunable swelling capacity utilizing hydrogel design parameters and external stimuli. In this study, poly(2-methacryloyloxyethyl phosphate) (PMOEP) hydrogels were synthesized via aqueous activators regenerated by electron transfer atomic transfer radical polymerization using ascorbic acid as the reducing agent. Swelling and deswelling behaviors of PMOEP hydrogels were examined in different salt solutions, pH conditions, and temperatures. The degree of swelling in salt solutions followed CaCl2 < MgCl2 < KCl < NaCl with a decrease in swelling rate at higher concentrations until reaching a saturation point. In water, the degree of swelling increased significantly around neutral pH and remained constant at basic pH values. The effects of polymerization conditions, including pH, temperature (30, 40, 50 °C), and MOEP concentration (40, 50, 60% v/v MOEP/H2O), on the hydrogel swelling behavior in various salt solutions were also investigated. PMOEP hydrogels showed a decrease in the degree of swelling as the pH was increased above the native pH of the monomer solution. Scanning electron microscopy and energy-dispersive spectroscopy were utilized to examine the microstructure and chemical composition of the dried hydrogel after salt solution swelling. Cytotoxicity testing using rat bone marrow stem cells confirmed the biocompatibility of the PMOEP hydrogels. A unique feature of this effort was evaluation of these phosphate hydrogels for use in expansion microscopy where a significant twofold enhancement in cellular expansion capacity was showcased utilizing 4T1 mouse breast cancer cells. This comprehensive study provides valuable insights into the stimuli-responsive behavior and expansion characteristics of phosphate hydrogels, highlighting their potential in diverse biomedical applications.

Investigating immune-modulatory function of α-glucopyranose-rich compound polysaccharides by MC38-N4/OT-I co-culture system

The potential antitumor function of polysaccharides is well accepted, it is unclear whether polysaccharides have immunoregulatory effect on CD8+ T lymphocyte cells to attack tumor cells. To evaluate the CD8+ T function enhancing role of polysaccharide compounds, the MC38-N4/OT-I co-culture system was established. The synergistic and complementary immune effect of α-glucopyranose-rich compound polysaccharides can be achieved by manipulating the antigen-specific T-cell expansion capacity and efficacy. This study was designed to investigate the antitumor-enhancement activity of a α-glucopyranose-rich compound polysaccharides by determining the activation of CD8+ T cells in a co-culture system. Compared to the control group (42.5 % ± 0.72 %), the specific α-glucopyranose-rich compound polysaccharides, comprising Agaricus blazei Murill, Grifola frondosa and Pericarpium Citri Reticulatae, demonstrated a significant decrease (20.4 % ± 1.23 %, p < 0.05) in the survival rate of MC38-N4 cells in the co-culture system. Additionally, the α-glucopyranose-rich compound polysaccharides resulted in a substantial increase (p < 0.01) in the proportion of CD8+ T cells and CD62L+ central memory T cells, which is a less differentiated T cell subset with high immune activity. Collectively, we reported that specific polysaccharide combination, which remodel the function of cytotoxic T cells and provided a basis for improving immune functions by using the specific types of polysaccharides.