Exogenous Cues Research Articles

Visuospatial Attention Allocation as an Indicator of Cognitive Deficit in Traumatic Brain Injury: A Systematic Review and Meta-Analysis

Traumatic Brain Injury (TBI) is defined by changes in brain function resulting from external forces acting on the brain and is typically characterized by a host of physiological and functional changes such as cognitive deficits including attention problems. In the present study, we focused on the effect of TBI on the ability to allocate attention in vision (i.e., the use of endogenous and exogenous visual cues) by systematically reviewing previous literature on the topic. We conducted quantitative synthesis of 16 selected studies of visual attention following TBI, calculating 80 effect size estimates. The combined effect size was large (g = 0.79, p < 0.0001) with medium heterogeneity (I2 = 68.39%). Subgroup analyses revealed an increase in deficit with moderate-to-severe and severe TBI as compared to mild TBI [F(2, 76) = 24.14, p < 0.0001]. Task type was another key source of variability and subgroup analyses indicated that higher order attention processes were severely affected by TBI [F(2, 77) = 5.66, p = 0.0051). Meta-regression analyses revealed significant improvement in visual attention deficit with time [p(mild) = 0.031, p(moderate-to-severe) = 0.002, p(severe) < 0.0001]. Taken together, these results demonstrate that visual attention is affected by TBI and that regular assessment of visual attention, using a systematic attention allocation task, may provide a useful clinical measure of cognitive impairment and change after TBI.

How the size of exogenous attentional cues alters visual performance: From response gain to contrast gain.

The normalisation model of attention (NMoA) predicts that the attention gain pattern is mediated by changes in the size of the attentional field and stimuli. However, existing studies have not measured gain patterns when the relative sizes of stimuli are changed. To investigate the NMoA, the present study manipulated the attentional field size, namely, the exogenous cue size. Moreover, we assessed whether the relative rather than the absolute size of the attentional field matters, either by holding the target size constant and changing the cue size (Experiments 1-3) or by holding the cue size constant and changing the target size (Experiment 4), in a spatial cueing paradigm of psychophysical procedures. The results show that the gain modulations changed from response gain to contrast gain when the precue size changed from small to large relative to the target size (Experiments 1-3). Moreover, when the target size was once again made larger than the precue size, there was still a change in response gain (Experiment 4). These results suggest that the size of exogenous cues plays an important role in adjusting the attentional field and that relative changes rather than absolute changes to exogenous cue size determine gain modulation. These results are consistent with the prediction of the NMoA and provide novel insights into gain modulations of visual selective attention.

Time-dependent inhibition of covert shifts of attention

Visual transients can interrupt overt orienting by abolishing the execution of a planned eye movement due about 90 ms later, a phenomenon known as saccadic inhibition (SI). It is not known if the same inhibitory process might influence covert orienting in the absence of saccades, and consequently alter visual perception. In Experiment 1 (n = 14), we measured orientation discrimination during a covert orienting task in which an uninformative exogenous visual cue preceded the onset of an oriented probe by 140–290 ms. In half of the trials, the onset of the probe was accompanied by a brief irrelevant flash, a visual transient that would normally induce SI. We report a time-dependent inhibition of covert orienting in which the irrelevant flash impaired orientation discrimination accuracy when the probe followed the cue by 190 and 240 ms. The interference was more pronounced when the cue was incongruent with the probe location, suggesting an impact on the reorienting component of the attentional shift. In Experiment 2 (n = 12), we tested whether the inhibitory effect of the flash could occur within an earlier time range, or only within the later, reorienting range. We presented probes at congruent cue locations in a time window between 50 and 200 ms. Similar to Experiment 1, discrimination performance was altered at 200 ms after the cue. We suggest that covert attention may be susceptible to similar inhibitory mechanisms that generate SI, especially in later stages of attentional shifting (> 200 ms after a cue), typically associated with reorienting.

The Incomplete Tyranny of Dynamic Stimuli: Gaze Similarity Predicts Response Similarity in Screen-Captured Instructional Videos.

Although eye tracking has been used extensively to assess cognitions for static stimuli, recent research suggests that the link between gaze and cognition may be more tenuous for dynamic stimuli such as videos. Part of the difficulty in convincingly linking gaze with cognition is that in dynamic stimuli, gaze position is strongly influenced by exogenous cues such as object motion. However, tests of the gaze-cognition link in dynamic stimuli have been done on only a limited range of stimuli often characterized by highly organized motion. Also, analyses of cognitive contrasts between participants have been mostly been limited to categorical contrasts among small numbers of participants that may have limited the power to observe more subtle influences. We, therefore, tested for cognitive influences on gaze for screen-captured instructional videos, the contents of which participants were tested on. Between-participant scanpath similarity predicted between-participant similarity in responses on test questions, but with imperfect consistency across videos. We also observed that basic gaze parameters and measures of attention to centers of interest only inconsistently predicted learning, and that correlations between gaze and centers of interest defined by other-participant gaze and cursor movement did not predict learning. It, therefore, appears that the search for eye movement indices of cognition during dynamic naturalistic stimuli may be fruitful, but we also agree that the tyranny of dynamic stimuli is real, and that links between eye movements and cognition are highly dependent on task and stimulus properties.

Temporal and spatial topography of cell proliferation in cancer.

3122 Background: Tumors are complex ecosystems where exogenous and endogenous cues are integrated to either stimulate or inhibit cancer cell proliferation. However, the nature of these complex cell cycle states, their spatial organization, response to perturbation, and implications for clinical outcomes, are poorly characterized in tumor tissues. Methods: We used multiplexed tissue imaging to develop a robust classifier of proliferation, the multivariate proliferation index (MPI), using 513 unique tumors across five cancer types. Next, we used dimensionality reduction analysis to assess how the patterns of cell cycle protein expression in tumors were altered in response to perturbation. Results: The MPI outperforms single markers, like Ki67, when classifying proliferative index across diverse tumor types and reveals the proliferative architecture of tumors in situ. We find that proliferative and non-proliferative cancer cells are organized across microscopic (cell-to-cell) and macroscopic (tissue-level) scales. Both domains are reshaped by therapy, and local clusters of proliferative and non-proliferative tumor cells preferentially neighbor distinct tumor-infiltrating immune cells. We further phenotyped non-proliferating cancer cells using markers of quiescent cancer cells, cancer stem cells, and dormant cancer cells. We found that these types of non-proliferating cancer cells can occupy distinct regions within the same primary tumor. In high-dimensional marker space, populations of proliferative cancer cells express canonical patterns of cell cycle protein markers, a property we refer to as “cell cycle coherence”. Untreated tumors exist in a continuum of coherence states, ranging from optimal coherence, akin to freely cycling cells in culture, to reduced coherence characterized by either cell cycle polarization or non-canonical marker expression. Coherence can be stereotypically altered by induction and abrogation of mitogen signaling in a HER2-driven model of breast cancer. Cell cycle coherence is modulated by neoadjuvant therapy in patients with localized breast cancer, and coherence is associated with disease-free survival after adjuvant therapy in patients with colorectal cancer, mesothelioma and glioblastoma. Conclusions: The MPI robustly defines proliferating and non-proliferating cells in tissues, with immediate implications for clinical practice and research. The coherence metrics capture the diversity of post-treatment cell cycle states directly in clinical samples, a fundamental step in advancing precision medicine. More broadly, replacing binary metrics with multivariate traits provides a quantitative framework to study temporal processes from fixed static images and to investigate the rich spatial biology of human cancers.

A multi-method assessment of attentional processes in chronic, treatment-resistant depression

Attentional deficits as well as attentional biases towards negative material are related to major depression and might maintain chronicity. However, studies investigating attentional deficits and attentional biases in chronic, treatment-resistant depressed are lacking. The aim of the current study was to compare measures of attentional deficits and attentional bias between chronic, treatment-resistant depressed outpatients and never-depressed control participants. Attentional deficits were assessed with the attentional control scale (ACS) and the Stroop Color naming task. Attentional bias was measured with the exogenous cueing task (ECT) and an emotional Stroop task. Chronic, treatment-resistant depressed patients (n = 80) showed significantly more attentional deficits than never-depressed controls (n = 113) on the ACS and Stroop color-naming task. However, in contrast with hypotheses, no differences were found between chronic, treatment-resistant depressed patients and never-depressed individuals on the ECT or emotional Stroop task. The current findings indicate that chronic, treatment-resistant depressed patients present attentional deficits. The results however question whether this patient group shows attentional biases for negative material. Future research should include comparisons of chronic, treatment-resistant and non-chronically depressed patients. If replicated, these current results might indicate that focusing on improving attentional deficits could be a more promising target for treatment than addressing attentional biases.

Neural Mechanisms of Reward-by-Cueing Interactions: ERP Evidence.

Inhibition of return (IOR) refers to the phenomenon that a person is slower to respond to targets at a previously cued location. The present study aimed to explore whether target-reward association is subject to IOR, using event-related potentials (ERPs) to explore the underlying neural mechanism. Each participant performed a localization task and a color discrimination task in an exogenous cueing paradigm, with the targets presented in colors (green/red) previously associated with high- or low-reward probability. The results of both tasks revealed that the N1, Nd, and P3 components exhibited differential amplitudes between cued and uncued trials (i.e., IOR) under low reward, with the N1 and Nd amplitudes being enhanced for uncued trials compared to cued trials, and the P3 amplitude being enhanced for cued trials vs. uncued trials. Under high reward, however, no difference was found between the amplitudes on cued and uncued trials for any of the components. These findings demonstrate that targets that were previously associated with high reward can be resistant to IOR and the current results enrich the evidence for interactions between reward-association and attentional orientation in the cueing paradigm.

The Modulation of Exogenous Attention on Emotional Audiovisual Integration.

Although emotional audiovisual integration has been investigated previously, whether emotional audiovisual integration is affected by the spatial allocation of visual attention is currently unknown. To examine this question, a variant of the exogenous spatial cueing paradigm was adopted, in which stimuli varying by facial expressions and nonverbal affective prosody were used to express six basic emotions (happiness, anger, disgust, sadness, fear, surprise) via a visual, an auditory, or an audiovisual modality. The emotional stimuli were preceded by an unpredictive cue that was used to attract participants’ visual attention. The results showed significantly higher accuracy and quicker response times in response to bimodal audiovisual stimuli than to unimodal visual or auditory stimuli for emotional perception under both valid and invalid cue conditions. The auditory facilitation effect was stronger than the visual facilitation effect under exogenous attention for the six emotions tested. Larger auditory enhancement was induced when the target was presented at the expected location than at the unexpected location. For emotional perception, happiness shared the biggest auditory enhancement among all six emotions. However, the influence of exogenous cueing effect on emotional perception seemed to be absent.

Improved Detection of Compound Arrhythmogenicity in 2D Cardiac Stem Cell Models via Advanced Maturation Strategies

The adoption of stem cells as relevant models of in vitro human function is often hampered by their lack of phenotypic maturity. For example, it has been shown that cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSC-CMs) can recapitulate some aspects of human toxicity in vitro, but fail to identify certain well-known safety liabilities. Bioengineering strategies hold great promise in advancing the maturity of a host of structural and functional phenotypes. This is especially relevant to cardiomyocytes, which are highly reliant on in vivo extracellular factors for their growth, development, and function. In this study, we hypothesized that advancing the in vitro maturation of hiPSC-CMs with a combination of exogenous cues will enable them to better detect known safety liabilities and better recapitulate in vivo biology based on industry-standard measures of assay fidelity. We used a novel microRNA-based maturation strategy that has been demonstrated to shift gene expression patterns in hiPSC-CMs to a more adult-like phenotype. We took these matured cells and treated them with several drugs from the CiPA formulary and benchmarked their measured risk score against unmatured cells. We measured the field potential duration and localized extracellular potential (LEAP) of the cells on MEAs. We tested bepridil, a known arrhythmogenic drug; in contrast to unmatured cells, matured myocytes showed clear FP prolongation (ddFPDc > 250 ms), clear Type-C arrhythmias in the field potential signal, and early afterdepolarizations in the LEAP signal. We also demonstrated that ranolazine, metoprolol, and mexiletine—which are safe in vitro but are typically scored as borderline dangerous in traditional myocyte assays—showed little to no toxicity (< 20 ms ddFPDc) and an absence of arrhythmias in matured preparations. We conclude that advancing hiPSC-CM maturation in vitro improves toxicity assay fidelity. The strategies presented are utilized in a cell, assay, and instrument-agnostic fashion and can be extended to nearly all hiPSC-CM models.

Transient perceptual enhancements resulting from selective shifts of exogenous attention in the central fovea

Exogenous attention, a powerful adaptive tool that quickly and involuntarily orients processing resources to salient stimuli, has traditionally been studied in the lower-resolution parafoveal and peripheral visual field.1-4 It is not known whether and how it operates across the 1° central fovea where visual resolution peaks.5,6 Here we investigated the dynamics of exogenous attention in the foveola. To circumvent the challenges posed by fixational eye movements at this scale, we used high-precision eye-tracking and gaze-contingent display control for retinal stabilization.7 High-acuity stimuli were briefly presented foveally at varying delays following an exogenous cue. Attended and unattended locations were just a few arcminutes away from the preferred locus of fixation. Our results show that for short temporal delays, observers' ability to discriminate fine detail is enhanced at the cued location. This enhancement is highly localized and does not extend to the nearby locations only 16' away. On a longer timescale, instead, we report an inverse effect: paradoxically, acuity is sharper at the unattended locations, resembling the phenomenon of inhibition of return at much larger eccentricities.8-10 Although exogenous attention represents a mechanism for low-cost monitoring of the environment in the extrafoveal space, these findings show that, in the foveola, it transiently modulates vision of detail with a high degree of resolution. Together with inhibition of return, it may aid visual exploration of complex foveal stimuli.11.

“Far from the mind”: Preliminary evidence of avoidance bias for emotional facial expressions among earthquake victims

Facial expressions are important threat cues that can induce attentional biases, particularly among anxious individuals, facilitating precautionary behaviour. However, no study has assessed earthquake victims for attentional bias related to emotional facial expressions. In this study, we set out to bridge this gap by testing biases for emotional facial expressions in relation to psychopathological symptoms in a group of victims of the 2009 L’Aquila (Italy) earthquake. In a between-subjects design, 14 earthquake victims and 14 controls completed an exogenous cueing task to assess attentional biases in respect of seven facial expressions (happy, sad, angry, disgust, surprise, fearful and neutral). In each trial, one face was briefly presented on the right or left side of the screen as a cue and was followed by a target (a small dot). Participants had to indicate the position of the target shown on the same side as the emotional face (valid trials) or the opposite side (invalid trials). MANOVA performed on the engagement and disengagement scores demonstrated that earthquake victims and controls differed only in the engagement scores for fearful expression. Moreover, single-sample t-tests demonstrated that victims showed attentional avoidance of both fearful and happy expressions. Finally, in earthquake victims, avoidance of fearful expressions negatively correlated with anxiety-related cognitive concerns. Although the results are preliminary, we provide evidence of attentional avoidance of emotional faces among earthquake victims that is present years after the traumatic event. Cognitive mechanisms underlying such a bias and their implications are discussed.

Repeated application of the covert shift of attention task improves endogenous but not exogenous attention in patients with unilateral visuospatial inattention

BackgroundMost neglect treatment studies focus on automatic re-orientation procedures, assuming a deficit in automatic processes. We compare an automatic- and a controlled procedure, using the endogenous and exogenous variants of Posner’s covert shift of attention task. MethodIn two experiments, neglect patients and patients with a right hemispherical stroke without neglect performed three blocks of Posner’s covert shift of attention task (Posner Task) on two days. In Study 1 we used endogenous cues, in Study 2, exogenous cues. ResultsIn the endogenous task, neglect patients improved significantly with valid left-sided cues between block 1 and 2 on Day 1, subsequently showing a plateauing. They also showed a gradual improvement on invalid trials on both days. In the exogenous condition, all participants responded only increasingly faster on trials with a long stimulus onset asynchrony. Practicing on both tasks led to fewer omissions for left-sided targets, minimally in the exogenous and clearly in the endogenous condition. ConclusionIn line with prior neuroanatomical studies, our study shows that practicing an endogenous, but not an exogenous, visuospatial attention task leads to significant improvements in neglect patients, especially for invalid trials, suggesting that neglect treatments based on top-down strategies should be given more attention.

Two chemically distinct root lignin barriers control solute and water balance

Lignin is a complex polymer deposited in the cell wall of specialised plant cells, where it provides essential cellular functions. Plants coordinate timing, location, abundance and composition of lignin deposition in response to endogenous and exogenous cues. In roots, a fine band of lignin, the Casparian strip encircles endodermal cells. This forms an extracellular barrier to solutes and water and plays a critical role in maintaining nutrient homeostasis. A signalling pathway senses the integrity of this diffusion barrier and can induce over-lignification to compensate for barrier defects. Here, we report that activation of this endodermal sensing mechanism triggers a transcriptional reprogramming strongly inducing the phenylpropanoid pathway and immune signaling. This leads to deposition of compensatory lignin that is chemically distinct from Casparian strip lignin. We also report that a complete loss of endodermal lignification drastically impacts mineral nutrients homeostasis and plant growth.

Exploiting Substrate Cues for Co-Culturing Cells in a Micropattern.

Spatial distribution of cells and their interactions between neighboring cells in native microenvironments are of fundamental importance in determining cell fate decisions such as migration, growth, and differentiation. Controlling the spatial distribution of different cell types in defined geometries can replicate these native environments, which can be a useful model for several studies. While spatiotemporal control over multiple cell arrangements is required to achieve the complex tissue architecture, unfortunately, conventional cell patterning techniques usually allow only single patterning with a single cell type. In the present study, we introduce a simple lithographic method to pattern multiple cell types in a spatially controlled manner by utilizing the biophysical cues present at the corners of the patterned geometry. By fabricating micropatterns of different shapes, we demonstrate how the cell can be constrained to pattern along the corners of patterned geometries owing to the presence of topographical cues, leaving empty voids in the center that can be further utilized for patterning a second cell type. We also demonstrate that the cell alignment along the pattern is a dynamic process and the cells migrate from a more uniform cell-adhesive region toward the topographical cues. The cytoskeleton arrangement was geometry-dependent, which was confirmed through a series of in vitro evaluations, such as scanning electron microscopy and fluorescence microscopy. These findings have not only helped us in exploring the importance of these cues in guiding the cell fate but have also allowed us to develop a technique, which self-patterns the cells without any expensive exogenous cues and can be used as a model protocol to eventually organize cells into a specific pattern with micron-scale precision in vitro.

Limited flexibility in departure timing of migratory passerines at the East-Mediterranean flyway

The rapid pace of current global warming lead to the advancement of spring migration in the majority of long-distance migratory bird species. While data on arrival timing to breeding grounds in Europe is plentiful, information from the African departure sites are scarce. Here we analysed changes in arrival timing at a stopover site in Israel and any links to Enhanced Vegetation Index (EVI) on the species-specific African non-breeding range in three migratory passerines between 2000–2017. Differences in wing length between early and late arriving individuals were also examined as a proxy for migration distance. We found that male redstart, but not females, advanced arrival to stopover site, but interestingly, not as a response to EVI phenology. Blackcap and barred warbler did not shift arrival timing significantly, although the arrival of blackcap was dependent on EVI. Barred warbler from the early arrival phase had longer wings, suggesting different populations. Our study further supports the existence species-specific migration decisions and inter-sexual differences, which may be triggered by both exogenous (local vegetation condition) and endogenous cues. Given rapid rate of changes in environmental conditions at higher latitudes, some migrants may experience difficulty in the race to match global changes to ensure their survival.

Therapeutic induction of tolerogenic dendritic cells via aryl hydrocarbon receptor signaling.

Dendritic cells (DCs) are potent antigen-presenting cells (APCs), which sample the exogenous and endogenous cues to control adaptive immunity, balancing effector and regulatory components of the immune response. Multiple subsets of DCs, such as plasmacytoid and conventional DCs, have been defined based on specific phenotypic markers, functions and regulatory transcriptional programs. Tolerogenic DCs (tolDCs) have been functionally defined based on their ability to expand the regulatory T-cell compartment and suppress immune responses. However, it is still unclear whether tolDCs represent a homogeneous population, a specific DC subset and/or a heterogeneous collection of DC activation/maturation states. The ligand-activated transcription factor aryl hydrocarbon receptor (AHR) has been shown to control transcriptional programs associated to tolDCs. In this review, we discuss the role of AHR in the control of tolDCs, and also AHR-targeted approaches for the therapeutic induction of tolDCs in autoimmune diseases and allergy.

Single-Case Neuropsychological Assessment of a Patient with a Posterior Parietal Lesion Using Behavioral Testing and Resting-State fMRI

This study integrated functional connectivity measures using resting-state fMRI and behavioral data from a single-case observation of patient (PER) one year after right-hemispheric hemorrhage in the intraparietal sulcus and superior parietal lobule (IPS/SPL). PER showed no sign of clinical neglect. Her behavioral performance in the visuo-manual pointing task and in the letter discrimination task under conditions of endogenous and exogenous attentional cueing was compared between the left (affected) and right (unaffected/control) peripheral visual fields. The resting-state fMRI demonstrated an imbalance between the right and left hemispheric frontoparietal functional connectivity within the dorsal attentional and motor networks. Although the frontal and occipital cortices were not structurally damaged, specific fronto-occipital functional connectivity was imbalanced, which was strongly associated with the behavioral changes. First, the activity in the right frontal eye field showed weaker correlations with the activity in the right inferior occipital area compared to the correlation with the activity in the left inferior occipital area. This imbalanced fronto-occipital functional connectivity was accompanied by a specific impairment in endogenous covert attention in the left visual field. Second, the activity within M1 in both hemispheres showed weaker correlations with the activity of the right cuneus compared to the correlation with the activity in the left cuneus. The imbalanced fronto-occipital functional connectivity was associated with the impairment of the reaching movement of the left and right hands towards the left visual field (optic ataxia). Altogether, our results showed that a lesion to the posterior parietal cortex affects the relationship between distal regions underlying the sensorimotor and attentional abilities

Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development.

Plants adjust their growth and development through a sophisticated regulatory system integrating endogenous and exogenous cues. Many of them rely on intricate crosstalk between nutrients and hormones, an effective way of coupling nutritional and developmental information and ensuring plant survival. Sugars in their different forms such as sucrose, glucose, fructose and trehalose-6-P and the hormone family of cytokinins (CKs) are major regulators of the shoot and root functioning throughout the plant life cycle. While their individual roles have been extensively investigated, their combined effects have unexpectedly received little attention, resulting in many gaps in current knowledge. The present review provides an overview of the relationship between sugars and CKs signaling in the main developmental transition during the plant lifecycle, including seed development, germination, seedling establishment, root and shoot branching, leaf senescence, and flowering. These new insights highlight the diversity and the complexity of the crosstalk between sugars and CKs and raise several questions that will open onto further investigations of these regulation networks orchestrating plant growth and development.

Nuclear envelope wrinkling predicts mesenchymal progenitor cell mechano-response in 2D and 3D microenvironments

Exogenous mechanical cues are transmitted from the extracellular matrix to the nuclear envelope (NE), where mechanical stress on the NE mediates shuttling of transcription factors and other signaling cascades that dictate downstream cellular behavior and fate decisions. To systematically study how nuclear morphology can change across various physiologic microenvironmental contexts, we cultured mesenchymal progenitor cells (MSCs) in engineered 2D and 3D hyaluronic acid hydrogel systems. Across multiple contexts we observed highly ‘wrinkled’ nuclear envelopes, and subsequently developed a quantitative single-cell imaging metric to better evaluate how wrinkles in the nuclear envelope relate to progenitor cell mechanotransduction. We determined that in soft 2D environments the NE is predominately wrinkled, and that increases in cellular mechanosensing (indicated by cellular spreading, adhesion complex growth, and nuclear localization of YAP/TAZ) occurred only in absence of nuclear envelope wrinkling. Conversely, in 3D hydrogel and tissue contexts, we found NE wrinkling occurred along with increased YAP/TAZ nuclear localization. We further determined that these NE wrinkles in 3D were largely generated by actin impingement, and compared to other nuclear morphometrics, the degree of nuclear wrinkling showed the greatest correlation with nuclear YAP/TAZ localization. These findings suggest that the degree of nuclear envelope wrinkling can predict mechanotransduction state in mesenchymal progenitor cells and highlights the differential mechanisms of NE stress generation operative in 2D and 3D microenvironmental contexts.

Lunar phase as a cue for migrations to two species of explosive breeding amphibians—implications for conservation

In species with explosive breeding strategies, large numbers of individuals may congregate at a defined location for a very short period of time. Effective synchronisation in arrival at breeding sites is crucial to ensure mating success. Amphibians with explosive breeding strategies often congregate at ponds for only a few days or weeks a year. Previous research has shown that frogs and toads may use a variety of exogenous cues to initiate breeding migrations which include temperature, rainfall and lunar cues. Although the effects of temperature and rainfall on amphibians are widely studied and understood, the impacts of lunar phase are poorly known and vary by species and location. In this study, we examined the effects of lunar phase on the numbers of common toads (Bufo bufo) and common frogs (Rana temporaria) migrating to breeding ponds at 43 sites across the UK over 4 years. Our findings show that peak migration of both common toads and common frogs coincides with the waxing phase of the moon, peaking around the full moon. Temperature and rainfall also had an effect on peak migrations with the highest numbers of common toads and common frogs occurring on warm and damp evenings close to a full moon. Our results have implications for amphibian conservation initiatives such as ‘Toads on Roads’ as they will help inform conservationists on the most effective timing to help toads and frogs across roads.