Exogenous Bacteria Research Articles

A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells

ObjectivesJunctional epithelium (JE) connects the tooth surface and gingival epithelium and adheres directly to the tooth enamel. JE plays an important role as a barrier preventing the invasion of exogenous bacteria and substances. However, the cellular characteristics of this epithelium have not been adequately described, because no useful in vitro experimental model exists for JE. MethodsWe generated a novel JE cell line, mHAT-JE01, using naturally immortalized dental epithelium derived from incisor labial cervical cells and by selecting cells that adhered to apatite. mHAT-JE01 was characterized by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction and compared with the gingival epithelial cell line, mOE-PE01. ResultsThe mHAT-JE01 cells had a higher capacity for producing JE-specific markers than oral mucous epithelial cells. In addition, the presence of lipopolysaccharides from Porphyromonas gingivalis downregulated the expression of JE protein markers in mHAT-JE01 cells. ConclusionsThis cell line is stable and presents the opportunity to characterize JE efficiently, which is essential for the prevention and treatment of periodontal disease.

Effect of tartary buckwheat sourdough fermented by different exogenous lactic acid bacteria on antifreeze property of frozen dough

In this study, tartary buckwheat combined with compound strains was fermented to make sourdough and applied to improve frozen dough. The results showed that the compound bacteria had a certain synergistic effect, and the bacterial colony growth in tartary buckwheat substrate was better than single bacteria, especially in LF+WC group, the lactic acid bacteria growth rate was the fastest, and a large amount of fructose (11.82 mmol/kg) and EPS (3.132 g/kg) were generated after 12 h fermentation. In addition, the binding water content of tartary buckwheat sourdough in LF+WC group decreased by 0.48%, content of non-flowing water and free water increased by 0.18% and 0.3%, respectively, the glutenin subunit degradation was less, α-helix and β-rotation content decreased by 0.43% and 2.34%, respectively, after 13 weeks of freezing. So that gluten can maintain a more uniform and regular network structure. Therefore, LF+WC tartary buckwheat sourdough has good antifreezing protection effect.

Molluscs-A ticking microbial bomb.

Bivalve shellfish consumption (ark shells, clams, cockles, and oysters) has increased over the last decades. Following this trend, infectious disease outbreaks associated with their consumption have been reported more frequently. Molluscs are a diverse group of organisms found wild and farmed. They are common on our tables, but unfortunately, despite their great taste, they can also pose a threat as a potential vector for numerous species of pathogenic microorganisms. Clams, in particular, might be filled with pathogens because of their filter-feeding diet. This specific way of feeding favors the accumulation of excessive amounts of pathogenic microorganisms like Vibrio spp., including Vibrio cholerae and V. parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Arcobacter spp., and fecal coliforms, and intestinal enterococci. The problems of pathogen dissemination and disease outbreaks caused by exogenous bacteria in many geographical regions quickly became an unwanted effect of globalized food supply chains, global climate change, and natural pathogen transmission dynamics. Moreover, some pathogens like Shewanella spp., with high zoonotic potential, are spreading worldwide along with food transport. These bacteria, contained in food, are also responsible for the potential transmission of antibiotic-resistance genes to species belonging to the human microbiota. Finally, they end up in wastewater, thus colonizing new areas, which enables them to introduce new antibiotic-resistance genes (ARG) into the environment and extend the existing spectrum of ARGs already present in local biomes. Foodborne pathogens require modern methods of detection. Similarly, detecting ARGs is necessary to prevent resistance dissemination in new environments, thus preventing future outbreaks, which could threaten associated consumers and workers in the food processing industry.

Using aerosols to decontaminate surfaces from nucleic acids

ABSTRACT The occurrence of cross-contamination, which leads to false-positive results when analyzing samples, is an important problem in the PCR laboratory. There are many ways to solve this problem, but none of them is universal. Treatment with aerosol is preferable for decontamination of large areas of complex surfaces. The goal of this study was to determine the efficiency of different modes of aerosol treatment for disinfection and nucleic acids decontamination. The solutions of compounds evolving active chlorine and active oxygen were used for decontamination of surfaces artificially contaminated with exogenous nucleic acids and bacteria. The potency of active ingredients of these solutions was assessed based on results of disinfection and nuclear acids decontamination. We have demonstrated effective modes of aerosol decontamination. The differences in conditions necessary for the nucleic acid decontamination and bacterial disinfection of laboratory surfaces are discussed.

Different phenanthrene degraders between free-cell mediated and biochar-immobilization assisted soil bioaugmentation as identified by RNA-based stable isotope probing (RNA-SIP)

Bioaugmentation (BA) is an effective approach to remove polycyclic aromatic hydrocarbons (PAHs) from contaminated soils, and biochar is frequently used to enhance PAH degradation performance. In this study, phenanthrene (PHE) degradation behavior and active degraders in a petroleum-contaminated soil were investigated and compared between free-cell mediated and biochar-immobilization assisted bioaugmentation. Biochar-immobilization assisted bioaugmentation (BA-IPB) introduced PHE degraders immobilized on biochar and effectively promoted PHE degradation, achieving higher PHE removal efficiencies within 24 h (~58 %) than free-cell mediated bioaugmentation (BA-FPB, ~39 %). Soil microbial community structure significantly changed in both BA-FPB and BA-IPB treatments. Through RNA-stable isotope probing (SIP), 14 and 11 bacterial lineages responsible for in situ PHE degradation were identified in BA-FPB and BA-IPB treatments, respectively. ASV_17 in BA-FPB treatment was Rhodococcus in the exogenous bacterial mixture; in contrast, none of exogenous bacteria were involved in PHE degradation in BA-IPB treatment. Methylobacterium (ASV_186), Xanthomonas (ASV_41), Kroppenstedtia (ASV_205), Scopulibacillus (ASV_243), Bautia (ASV_356), and Lactobacillus (ASV_376) were identified as PHE degraders for the first time. Our findings expanded the knowledge of the active PHE degraders and underlying mechanisms in bioaugmentation process, and suggested biochar-immobilization assisted bioaugmentation as a promising strategy for the bioremediation of PAH contaminated soils.

Effect of Burkholderia ambifaria LK-P4 inoculation on the plant growth characteristics, metabolism, and pharmacological activity of Anoectochilus roxburghii.

Plant growth-promoting bacteria (PGPB) represents a common biological fertilizer with remarkable effect in improving crop production and environmental friendliness. In the present work, we presented a detailed characterization of plant morphology and physiology, metabolism, and pharmacological activity of A. roxburghii between Burkholderia ambifaria LK-P4 inoculation and un-inoculation (CK) treatment by routine analytical techniques (include microscopy and enzymatic activity assays and so on) coupled with metabolomics approaches. Morphological and physiological results showedthat the P4 bacteria could significantly increase plant stomatal density, freshweight, survival rate,and the content of total flavonoids in leaves but reducethe amount of free amino acid. Furthermore, metabolite data showed that fatty acids (linoleic acid, linolenic acid, stearic acid) and active substance (kyotorphin and piceatannol) were specifically up-regulated in P4 inoculation. It was also demonstrated that the differential metabolites were involved in citrate cycle, glyoxylate and dicarboxylate metabolism, and biosynthesis of unsaturated fatty acids pathway. In addition, pharmacological efficacy found that A. roxburghii under P4 inoculation can significantly decrease (p < 0.05) blood glucose levels and protect the organs of mice with similar effect of Glibenclamide tablets. Overall, it can be seen that the exogenous P4 bacteria can promote the growth and increase content of special metabolites in A. roxburghii. This study provided theoretical basis and supported for the high-yield and high-quality bionic cultivation of A. roxburghii.

A novel chitosan-biochar immobilized microorganism strategy to enhance bioremediation of crude oil in soil

The chitosan-biochar composite is a clean and environmentally friendly immobilized microorganisms carrier. In this study, the chitosan-biochar composite as a carrier to immobilize a compound microbial agent contained Pseudomonas aeruginosa and Bacillus licheniformis, and investigated its role in the remediation of oil-contaminated soil. When using 1% (v/v) acetic acid, 3% (m/v) chitosan solution, 0.1% biochar, 4% (v/v) NaOH solution, freeze-drying 6 h, the optimal chitosan-biochar composite material could be obtained. The specific surfacearea of the material increased to 1.725 m2/g and the average pore size also increased from 130.2260 nm to 165.2980 nm after the addition of biochar through the analysis of specific surface area and pore size, which enlarged the contact area of microorganisms and crude oil with the material. SEM showed that the bacterial successfully adhered to the surface and internal of the material. Using FTIR, the results showed that the synthesis of composite carrier material was the covalent combination of –NH2 on chitosan and –COOH on biochar, forming a new chemical bond –NH–CO-. After 60 days of remediation of oil-contaminated soil, the removal rate of crude oil by chitosan-biochar composite immobilized microorganism method was 45.82%, which was 21.26% higher than that of natural remediation. Simultaneously, several oil-degrading bacteria increased at genus level, including Nocardioides (26.79%–33.09%), Bacillus (3.01%–4.10%), Dietzia (1.84%–5.56%), Pseudomonas (0–0.78%), among which Pseudomonas belongs to exogenous bacteria. The results indicated that the chitosan-biochar composite material has high application value in removing crude oil, and further provides a new strategy for bioremediation of oil-contaminated soil.

The exogenous compound bacteria alter microbial community and nutrients removal performance in the biofilm unit of the integrated aquaculture wastewater bioremediation systems

Integrated bioremediation systems are widely emerging treatment of mariculture wastewaters. The system consists of several units including the biofilm which is microbial consortium that help in removal of waste nutrients. However, a great performance would have been achieved if there was an addition of exogenous compound bacteria (ECB) in the biofilm unit. In this study, the ECB were added to the biofilm unit of an artificially created integrated mariculture wastewater system to explore their influence on the microbial community composition and structural diversity. A high-throughput amplicon of 16 S rRNA, PERMANOVA and LDA Effect Size (LEfSe) analyses were used to investigate the effects of ECB on the microbial community and nutrients removal performance in the biofilm unit of the integrated bioremediation systems of aquaculture wastewater (ISBAW). The results showed that the contents of total phosphorus (TP), chemical oxygen demand (CODMn), ammonia nitrogen (NH4+-N), and nitrate (NO3--N) decreased significantly after the treatment of ECB with the removal rates of 68%, 58%, 75% and 84%, respectively. The bacterial community in the water column has experienced three successional stages: the pretreatment, the middle and the late stage. The biofilm has gone through two successional stages: the pretreatment - early stage and the late stage. Bacillus became potential biomarker in the late water column and the pretreatment biofilm, respectively. Random processes were closed correlated with the community in the water column while deterministic processes were closely correlated to the biofilm’s component. Generally, the addition of ECB significantly affects the structural composition of bacterial communities on different media in the biofilm unit. The study provides basic information for understanding and development of efficient and optimized the IBSAW.

Involvement of exogenous arsenic-reducing bacteria in root surface biofilm formation promoted phytoextraction of arsenic

Root surface biofilm (RSB) is the last window for pollutants entering plant roots and thus plays a critical role in the phytoextraction of pollutants. Exogenous arsenic-reducing bacteria (EARB) have been adopted to enhance the phytoextraction of arsenic (As). However, whether EARB would be involved in RSB formation together with indigenous bacteria and the role of EARB involvement in As phytoextraction are still unknown. Herein, two EARB strains and two phytoextractors (wheat and maize) were selected to investigate the involvement of EARB in RSB formation and its role in As phytoextraction. Results showed that EARB successfully participated in RSB formation together with indigenous bacteria, attributing to their strong chemotaxis and biofilm formation abilities induced by root exudates. The involvement of EARB in RSB formation significantly enhanced As accumulation in plant roots, since more arsenite (As(III)) caused by arsenate (As(V)) reduction in RSB was absorbed by roots. Its underlying mechanism was further elucidated. EARB involvement increased phylum Proteobacteria to produce more siderophores in RSB. Siderophores then improved photosynthesis by increasing catalase and peroxidase activities and decreasing the malondialdehyde of plants. These actions further raised the shoot fresh weight to enhance As accumulation in plant roots. Moreover, mesophyll cell in wheat has a stronger As(V) reduction ability than that in maize, resulting in opposite distribution patterns of As(III) and As(V) in wheat and maize shoots. This study provides a new understanding of phytoextraction enhanced by exogenous bacteria and fills the gap in the role of EARB in As phytoextraction from the perspective of the RSB microregion.

Recovery trajectories of the bacterial community at distances in the receiving river under wastewater treatment plant discharge

Microbes in rivers are an important part of the biogeochemical cycle in aquatic ecosystems, and understanding the major factors that influence the composition of microbial communities has an important role in assessing and improving ecosystem functioning. A high-throughput 16 S rRNA gene sequencing technique was employed to sequence bacterial communities in 21 sediment samples and 21 water samples from an urban river WWTP (wastewater treatment plant) discharge. A systematic study of changes in bacterial community composition in downstream river sediment and water was conducted. The study found that compared with the bacterial diversity in the natural upstream area of the wastewater outfall, the bacterial diversity in the sediment lower reaches decreased significantly, while the bacterial abundance and diversity in the water increased significantly. The Mantel test and redundancy analysis showed that the downstream distance and physicochemical properties were significantly related to the succession of bacterial communities in the sediment downstream of the WWTP discharge. Among them, TOC (total organic carbon) was the most important factor affecting the change in the bacterial community in the downstream sediment. The physicochemical properties were significantly correlated with the succession of bacterial communities in the water downstream of the WWTP discharge. Among them, TN (total nitrogen), PO43--P (phosphorus phosphate) and TP (total phosphorus) were the main factors that affected the change in the bacterial community in the downstream water. Key taxa in the co-occurrence network at different distances downstream reflected the depth of the effect of the WWTP effluent on the bacterial community. The bacterial community in the lower reaches of the river sediment showed a strong recovery ability under the influence of pollutants, while the bacterial community in the lower reaches of the river water was difficult to recover under the influence of pollutants. In general, pollutants contained in effluent are the key to changing the composition of bacterial communities in the lower reaches of the river, but exogenous bacteria in effluent are not. This study provides a basis for further improving the effluent discharge standards of WWTPs in the future.

Exploring plant growth promoting rhizobacteria potential for green agriculture system to optimize sweet potato productivity and soil sustainability in northern Jiangsu, China

The beneficial effects of plant growth-promoting rhizobacteria (PGPR) on crop growth and yield have been widely recognized. However, the application of PGPR in traditional planting systems is often restricted due to the concerns about the incompatibility between PGPR and chemical agricultural inputs. The aim of this study is to test whether the application of PGPR has a beneficial effect on the productivity and sustainability of the green planting system, where the use of low-toxic chemical pesticides and proper amount of chemical fertilizer is allowed. In the current study, field trials of four agriculture systems: green agriculture system, green agriculture system supplemented with beneficial microorganism consortium, organic agriculture system supplemented with the consortium and the control system, were carried out with white, yellow and purple flesh sweet potato varieties. The application of PGPR increased the plant growth and suppressed the stem nematode disease in green system. As a consequence, PGPR increased the yield by 26.44% and raised the farmers’ projected returns in green system on an average of the two-year assessment. Moreover, PGPR promoted the product nutritive quality by 7.51% in soluble sugar and 14.30% in vitamin C content, and also increased the anthocyanin content of the purple sweet potato by 10.73%. Exogenous PGPR significantly retarded the depletion of soil fertility during the crop cultivation, promoted the soil bacterial diversity and altered the bacteria community structure. PGPR treatment increased the relative abundances of Pseudomonas and Lysobacter in the rhizosphere soil, while reduced the amounts of Erwinia and Ralstonia. The changes of soil nutrient and microbes were correlated with the application of exogenous beneficial bacteria regardless of in organic or green agriculture system. Here, we showed the ability of PGPR to efficiently improve the productivity in green agriculture system under the tested condition, and provided evidence for the feasibility of using exogenous PGPR to achieve agricultural sustainability by constructing a positive feedback in crop-soil-microbe interactions.

Effects of epiphytic and exogenous lactic acid bacteria on fermentation quality and microbial community compositions of paper mulberry silage.

This study aimed to isolate, characterize, and identify lactic acid bacteria (LAB) strains from various sources and evaluate their effects on the nutritional quality, fermentation characteristics, and microbial compositions of paper mulberry (PM) after 60 days of ensiling. Forty-nine LAB strains were isolated from Phalaris arundinacea silage, pickle, and fresh PM leaves; three of these strains (Lactiplantibacillus plantarum, YC1; Levilactobacillus brevis, PC3; and Lactiplantibacillus plantarum, BP17) and one commercial inoculant Gaofuji (GFJ) were subsequently used. Compared with other treatments, PC3 and BP17 increased (P < 0.05) the LAB count and crude protein content and decreased (P < 0.05) the molds and coliform bacteria counts, pH, and ammonia-N content of PM silages. BP17 and PC3 increased the relative Lactiplantibacillus abundance and decreased that of Lelliottia and Cladosporium, improving PM silage quality. Therefore, PC3 and BP17 can improve the fermentation quality of PM silage and could be used as silage starter cultures.

Effects of probiotics on growth, the toll-like receptor mediated immune response and susceptibility to Aeromonas salmonicida infection in rainbow trout Oncorhynchus mykiss

This study evaluated the effects of probiotics on growth, survival, and toll-like receptors (TLRs)-mediated immune response and susceptibility to Aeromonas salmonicida infection in the rainbow trout (Oncorhynchus mykiss). Probiotic bacterium Bacillus velezensis MVCR2 strain isolated from turbot and Latilactobacillus sakei RDB2 strain isolated from rainbow trout were administered. A 30-day feeding experiment was conducted with three diet treatments: diet supplemented with B. velezensis (BV group), diet supplemented with L. sakei (LS group), and diet without probiotic supplementation as a control. Rainbow trout supplemented with probiotics benefitted by gaining body weight and resistance to A. salmonicida. The expression levels of TLRs (1, 2, 3, 4, 5, 7, 9, and 22) and the related signaling cascade pathways were determined. Upregulation of TLRs (1, 2, 3, 4, 7, 9, and 22) and the signaling cascade genes myd88 and irak4 was detected in different organs after ten days of feeding in the two groups. Interestingly, most of the upregulated TLRs on day ten recovered or even showed downward regulation after 30 days of feeding. Notably, the expression levels of all selected TLRs in the BV and LS groups were significantly upregulated in the kidneys and spleens after the A. salmonicida challenge. Our results suggest that different TLRs are involved in the recognition of exogenous and indigenous beneficial bacteria. Moreover, probiotics were capable of enhancing innate immune function in rainbow trout by training TLR-mediated MyD88-independent pathways, thus helping the host to respond rapidly to pathogen invasion. These findings lay the foundation for future studies on the mechanism by which probiotics regulate fish hosts and provide scientific basis for the use of probiotics in healthy rainbow trout culture.

Sodium butyrate attenuates bovine mammary epithelial cell injury by inhibiting the formation of neutrophil extracellular traps

Neutrophil extracellular traps (NETs) are an important means by which the body fights against exogenous bacteria. However, studies have shown that excessive NETs release can damage other cells. Accumulating evidence has shown that butyric acid can alleviate the inflammatory response of cells. However, the effect of butyric acid on Staphylococcus aureus-induced NETs formation and its underlying mechanism are still unclear. In this study, western blotting, immunofluorescence and CCK-8 assays were used to examine the effect of NETs formation by sodium butyrate (NaB). The results showed that NaB suppressed the release of S. aureus-induced NETs formation, as indicated by decreases in the levels of DNA, histones, myeloperoxidase, and neutrophil elastase. S. aureus can induce autophagy, and autophagy plays a key role in the formation of NETs. Our data showed that NaB activated mammalian target of rapamycin (mTOR) and the kinases protein kinase B (AKT) and unc-51 like kinase 1 (ULK1) at Ser757 and inhibited AMP-activated protein kinase (AMPK). To explore whether NaB inhibited the formation of NETs by inhibiting autophagy, we added 3-methyladenine (autophagy inhibitor) (3-MA, 5 mM) to bovine neutrophils, and the results showed that 3-MA significantly inhibited NETs release. Furthermore, we found that NETs and their component histones exhibited significantly increased the cytotoxic effects on bovine mammary epithelial cells (BMECs), indicating that NETs and their component histones play a key role in BMEC damage. In conclusion, NaB can reduce the excessive formation of NETs by inhibiting autophagy, thus reducing the damaging effect of NETs on BMECs.

Bioprocess of Oil Contaminated Water At Gathering Station Through A Consortium Of Endogenous and Exogenous Bacteria

Production activities of petroleum have resulted in extensive environmental pollution, due to accidents, leaks, and oil spills during these activities. Gathering Station is one production facility at the oil field that serves as a gathering place for several liquids produced from production wells to measure the production flow rate. Wastewater at the Gathering Station that still contains oil, so before being discharged into water bodies should be treated to match the existing quality standards. One of the promising methods to remove the oil-polluted at gathering stations is the use of bioprocess technology, which is an eco-friendly, cost-effective, and sustainable approach. A laboratory study was carried out on the Bioprocess of wastewater contaminated oil at the gathering through a consortium of endogenous bacteria and Bacillus cereus and Pseudomonas aeruginosa as consortium exogenous bacteria. The major objective of this research is to determine the effectiveness of consortium endogenous bacteria and consortium exogenous bacteria (Bacillus cereus and Pseudomonas aeruginosa) in degrading oil at the gathering station. Bioprocess was done by using 10% (v/v) inoculum consortium of endogenous bacteria and 10% (v/v) inoculum Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and using oil spill dispersant (OSD) 0.2% (v/v) in media. Oil concentrations were determined by the gravimetric method (SNI 6989.10-2011). The result showed that the mixture of Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and consortium endogenous bacteria in media produced the highest value of efficiency in degradation oil that up to 64.29% during 21 days (from 1062 mg/l to 379.25 mg/l), with a total population 65 x 106 cfu/ml, this indicated a synergistic relationship between Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and consortium endogenous bacteria at room temperature (21±1°C), pH range value 8.34-9.68 and Total Dissolved Solids (TDS) range value from 553-167 mg/l.

Persistence of fermented food bacteria in the oral cavity of rats after one week of consumption

Fermented foods constitute a wide source of exogenous lactic acid bacteria (LAB) which could modify the endogenous microbiota of the consumer's digestive tract. This assumption depends on the ability of LAB to persist, at least transiently, in the oral cavity. The aim of this study was to evaluate the persistence of 3 LAB species, Streptococcus thermophilus, Lactococcus lactis, and Lacticaseibacillus paracasei in the oral cavity of rats by feeding them with a daily portion of model cheese containing the three bacteria for one week. Saliva samples were collected 16 h and one week after the last daily cheese intake. Their cheese bacteria contents were quantified by qPCR. When 16 h after cheese consumption, cheese bacteria were detected in the oral cavity of about ⅓ of the rats ranging from 10−3% to 10−1% of the total bacteria. One week after the last cheese intake, 1/8 LAB remains in the oral cavity. This study is a proof of concept showing that LAB can remain in the oral cavity several days after fermented food consumption with variability depending on individuals.

Characterization of siderophores from Escherichia coli strains through genome mining tools: an antiSMASH study

Although urinary tract infections (UTIs) affect many people, they are usually a disease observed in women. UTIs happen when exogenous and endogenous bacteria enter the urinary tract and colonize there. Cystitis and pyelonephritis occur when bacteria infect the bladder and the kidneys, respectively. UTIs become much serious if the bacteria causing the infection are antibiotic resistant. Since the pathogenic microorganisms have been adopted to current antibiotics via genetic variations, UTIs have become an even more severe health problem. Therefore, there is a great need for the discovery of novel antibiotics. Genome mining of nonpathogenic and pathogenic Escherichia coli strains for investigating secondary metabolites were conducted by the antiSMASH analysis. When the resulting secondary metabolites were examined, it was found that some of the siderophores are effective in UTIs. In conclusion, since the siderophore production in E. coli is directly related to UTIs, these molecules can be a good target for development of future pharmaceutical approaches and compounds. Siderophores can also be used in industrial studies due to their higher chelating affinity for iron.

Effects of exogenous cellulose-degrading bacteria on humus formation and bacterial community stability during composting

This study aimed to reveal the potential mechanism of influence exogenous cellulose-degrading bacteria (ECDB) exerted on humus synthesis during the co-composting of corn straw and cattle manure. By measuring the changes in physicochemical factors and bacterial communities, it was revealed that inoculation with ECDB enhanced the driving force of cellulose degradation and humus synthesis. ECDB not only directly participated in cellulose degradation as degrading bacteria, but also changed the bacterial community succession, and increased the abundance of bacterial communities associated with cellulose degradation. The results showed that ECDB stimulated the potential functions and interactions of bacterial communities. Structural equation modeling confirmed that ECDB acted mainly as a bioactivator to promote humus formation in co-composting of corn straw and cattle manure. Taken together, these findings offered new strategies which can be effectively utilized to increase the efficiency and quality of corn straw composting.

Phagocytosis of exogenous bacteria by gill epithelial cells in the deep-sea symbiotic mussel Bathymodiolus japonicus.

Animals that live in nutrient-poor environments, such as the deep sea, often establish intracellular symbiosis with beneficial bacteria that provide the host with nutrients that are usually inaccessible to them. The deep-sea mussel Bathymodiolus japonicus relies on nutrients from the methane-oxidizing bacteria harboured in epithelial gill cells called bacteriocytes. These symbionts are specific to the host and transmitted horizontally, being acquired from the environment by each generation. Morphological studies in mussels have reported that the host gill cells acquire the symbionts via phagocytosis, a process that facilitates the engulfment and digestion of exogenous microorganisms. However, gill cell phagocytosis has not been well studied, and whether mussels discriminate between the symbionts and other bacteria in the phagocytic process remains unknown. Herein, we aimed to investigate the phagocytic ability of gill cells involved in the acquisition of symbionts by exposing the mussel to several types of bacteria. The gill cells engulfed exogenous bacteria from the environment indiscriminately. These bacteria were preferentially eliminated through intracellular digestion using enzymes; however, most symbionts were retained in the bacteriocytes without digestion. Our findings suggest that regulation of the phagocytic process after engulfment is a key mechanism for the selection of symbionts for establishing intracellular symbiosis.

Integrated network analysis reveals that exogenous cadmium-tolerant endophytic bacteria inhibit cadmium uptake in rice

Most previous studies have focused on the diversity and species richness of microbial communities, however, understanding the interactions between species and detecting key functional members of the community can help us better understand how microorganisms perform their functions. In this study, the response of the rice plant microbial community to the inoculation of cadmium-resistant endophytic bacterium R5 (Stenotrophomonas) was investigated for the first time using a microbial phylogenetic molecular ecological network. The results showed that inoculation of R5 changed the topological characteristics of the microbial network in rice plants, with the resulting network displaying stronger complexity and interaction in roots and aboveground parts, indicating that inoculation of R5 provided favorable conditions for microbial interactions. In addition, these interactions may be related to the absorption and transportation of cadmium by rice. Under the exogenous addition of R5, the network interactions of the rice plant microbial community were more inclined to cooperation. Both in the roots and aboveground parts of rice, the plant Cd content showed a decrease as the complexity and connectivity of the network increased, suggesting that complex microbial networks may be more beneficial to rice than simple microbial networks because as they were more adaptive and resistant to unfavorable environments. After inoculation with the R5 strain, the negative interaction with Cd content in rice plants increased significantly, and there might be more synergy between the microbial community and plants to jointly inhibit the absorption and transportation of Cd.