Bioprocess of Oil Contaminated Water At Gathering Station Through A Consortium Of Endogenous and Exogenous Bacteria

Abstract

Production activities of petroleum have resulted in extensive environmental pollution, due to accidents, leaks, and oil spills during these activities. Gathering Station is one production facility at the oil field that serves as a gathering place for several liquids produced from production wells to measure the production flow rate. Wastewater at the Gathering Station that still contains oil, so before being discharged into water bodies should be treated to match the existing quality standards. One of the promising methods to remove the oil-polluted at gathering stations is the use of bioprocess technology, which is an eco-friendly, cost-effective, and sustainable approach. A laboratory study was carried out on the Bioprocess of wastewater contaminated oil at the gathering through a consortium of endogenous bacteria and Bacillus cereus and Pseudomonas aeruginosa as consortium exogenous bacteria. The major objective of this research is to determine the effectiveness of consortium endogenous bacteria and consortium exogenous bacteria (Bacillus cereus and Pseudomonas aeruginosa) in degrading oil at the gathering station. Bioprocess was done by using 10% (v/v) inoculum consortium of endogenous bacteria and 10% (v/v) inoculum Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and using oil spill dispersant (OSD) 0.2% (v/v) in media. Oil concentrations were determined by the gravimetric method (SNI 6989.10-2011). The result showed that the mixture of Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and consortium endogenous bacteria in media produced the highest value of efficiency in degradation oil that up to 64.29% during 21 days (from 1062 mg/l to 379.25 mg/l), with a total population 65 x 106 cfu/ml, this indicated a synergistic relationship between Bacillus cereus and Pseudomonas aeruginosa as exogenous bacteria and consortium endogenous bacteria at room temperature (21±1°C), pH range value 8.34-9.68 and Total Dissolved Solids (TDS) range value from 553-167 mg/l.