Airborne particles, bacteria and fungi consist main determinants of indoor air quality. In this study, these characteristics were investigated in two exhibition halls of the Historical Museum of Crete, in comparison with the outdoor environment. In Zacharias Portalakis (ZP) hall, three air purifiers were operating during museum opening hours, as opposed to El Greco (EG) hall. A significant part of ultrafine (< 1 μm) particles was efficiently removed by the air purifiers in ZP hall. Airborne coarse particles PN2.5–10 were associated with human occupancy in both halls, indicating transportation from visitors and resuspension as possible sources. Average airborne bacterial concentration was also lower in ZP than in EG hall, assessed by both molecular and culture-dependent methods. The bacterial and fungal communities of both indoor halls were distinct from the outdoor counterpart. Micrococcus and Staphylococcus, in terms of bacteria, and Alternaria and Malassezia, in terms of fungi, were the most abundant genera indoors, most of them being human-related. Hierarchical clustering of indoor samples indicated that EG hall bacteria were similar to ZP hall bacteria collected on the same day, but varied between different sampling dates. This observation, together with the bacterial beta-diversity analysis, implied that both indoor halls probably shared common bacterial source(s), while the respective fungal pattern of the two indoor halls was found significantly separated. The outdoor air contribution in EG and ZP hall bacterial profile presented a comparable fluctuation pattern; this was not observed for fungi, probably indicating the complexity of potential sources for different fungal taxa.
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