Exercise Training Status Research Articles

Heart Rate Variability in Basketball: The Golden Nugget of Holistic Adaptation?

The main aim of this narrative review is to assess the existing body of scientific literature on heart rate variability (HRV) in relation to basketball, focusing on its use as a measure of internal load and vagal nerve responses. Monitoring HRV offers insights into the autonomic function and training-induced adaptations of basketball players. Various HRV measurement protocols, ranging from short-term to longer durations, can be conducted in different positions and conditions, such as rest, training, and sleep, to determine this key metric. Consistency and individualization in measurement protocols, responding to the athlete’s specific characteristics, is crucial for reliable HRV data and their interpretation. Studies on HRV in basketball have explored psychological adaptation, training effects, individual differences, recovery, and sleep quality. Biofeedback techniques show positive effects on HRV and anxiety reduction, potentially enhancing performance and stress management. The scientific literature on HRV in basketball could benefit from studies involving longer monitoring periods to identify significant trends and results related to training and recovery. Longitudinal HRV monitoring in teams with intense travel schedules could reveal the impact on athletes of all levels and ages, and, in this regard, individualized interpretation, considering the subjective recovery and fitness levels of athletes, is recommended to optimize training programs and performance. HRV provides insights into training and competitive loads, aiding in determining exercise intensities and training status. Additionally, HRV is linked to recovery and sleep quality, offering valuable information for optimizing player performance and well-being. Overall, HRV is a reliable tool for adjusting training programs to meet the specific needs of basketball players.

The Influence of Age and Exercise Training Status on Left Ventricular Systolic Twist Mechanics in Healthy Males-An Exploratory Study.

Age-related differences in twist may be mitigated with exercise training, although this remains inconclusive. Moreover, temporal left ventricular (LV) systolic twist mechanics, including early-systolic (twistearly), and beyond peak twist (twistpeak) alone, have not been considered. Therefore, further insights are required to ascertain the influence of age and training status on twist mechanics across systole. Forty males were included and allocated into 1 of 4 groups based on age and training status: young recreationally active (YRA, n = 9; 28 ± 5 years), old recreationally active (ORA, n = 10; 68 ± 6 years), young trained (YT, n = 10; 27 ± 6 years), and old trained (OT, n = 11, 64 ± 4 years) groups. Two-dimensional speckle-tracking echocardiography was performed to determine LV twist mechanics, including twistearly, twistpeak, and total twist (twisttotal), by considering the nadir on the twist time-curve during early systole. Twisttotal was calculated by subtracting twistearly from their peak values. LV twistpeak was higher in older than younger men (p = 0.036), while twistpeak was lower in the trained than recreationally-active (p = 0.004). Twistpeak is underestimated compared with twisttotal (p < 0.001), and when early-systolic mechanics were considered, to calculate twisttotal, the age effect (p = 0.186) was dampened. LV twist was higher in older than younger age, with lower twist in exercise-trained than recreationally-active males. Twistpeak is underestimated when twistearly is not considered, with novel observations demonstrating that the age effect was dampened when considering twistearly. These findings elucidated a smaller age effect when early phases of systole are considered, while lower LV systolic mechanics were observed in older aged trained than recreationally-active males.

Long-term self-reported attendance in exercise training or lung choir and status of quality of life following initial pulmonary rehabilitation for COPD.

Both adherence rates to pulmonary rehabilitation (PR) programmes and long-term attendance in exercise training after PR remain a challenge. In our previous randomised controlled trial (RCT), effects were positively associated with a dose-response pattern, regardless of whether PR contained conventional physical exercise training (PExT) or Singing for Lung Health (SLH) as a training modality within a 10 weeks' PR programme for chronic obstructive pulmonary disease (COPD). However, long-term status of this RCT cohort remains unknown. In this study, we investigated whether current status (=attendance in supervised exercise training or a lung choir and scoring in quality of life (QoL)) was related to initial PR completion, randomisation, or adherence. We collected data via telephone, using a researcher-developed questionnaire on current self-reported attendance in supervised exercise training or a lung choir and on perceived benefits of the initial RCT intervention. Additionally, we used COPD-validated questionnaires (primarily: QoL (measure: St George's Respiratory Questionnaire; SGRQ). In 2023 (i.e., mean/median 4.7 years after initial PR), surviving participants were contacted (n = 196; 73% of 270), and 160 (82% of 196) were included. Out of the included participants, 30 (19%) had not completed initial PR. Compared to the initial PR-completers, non-completers reported less current attendance in exercise training or lung choir (24% vs. 46%, p = 0.03) but SGRQ scores were comparable. Yet, those who attended exercise training or lung choir at present (n = 66/160; 41% out of 160) reported better QoL score than those with no current attendance (SGRQ; Attending: 39.9 ± 15.4; Not attending: 43.1 ± 16.7; p = 0.02). Neither having had SLH instead of PExT, nor adherence level during initial PR, was related to current attendance or to QoL scores. This study indicates that long-term self-reported attendance and current QoL scores are positively related to initial completion of a PR programme. Surprisingly, neither initial PR content (PExT or SLH) nor initial PR adherence was related to long-term outcomes. We suggest that future PR programmes include special attention to those who do not complete PR to support long-term attendance and QoL status.

The acute effects of motor cortex transcranial direct current stimulation on athletic performance in healthy adults: A systematic review and meta-analysis.

This systematic review and meta-analysis assesses independently the acute effects of anodal and cathodal motor cortex transcranial direct current stimulation (tDCS) on athletic performance in healthy adults. Besides, it evaluates the unique and conjoint effects of potential moderators (i.e., stimulation parameters, exercise type, subjects' training status and risk of bias). Online database search was performed from inception until March 18th 2024 (PROSPERO: CRD42023355461). Forty-three controlled trials were included in the systematic review, 40 in the anodal tDCS meta-analysis (68 effects), and 9 (11 effects) in the cathodal tDCS meta-analysis. Performance enhancement between pre- and post-stimulation was the main outcome measure considered. The anodal tDCS effects on physical performance were small to moderate (g= .29, 95%CI [.18, .40], PI = -.64 to 1.23, I2= 64.0%). Exercise type, training status and use of commercial tDCS were significant moderators of the results. The cathodal tDCS effects were null (g = .04, 95%CI [-.05, .12], PI = -.14 to .23, I2= 0%), with a small to moderate heterogeneity entirely due to sampling error, thus impairing further moderator analysis. These findings hold significant implications for the field of brain stimulation and physical performance, as they not only demonstrate a small to moderate effect of acute tDCS but also identify specific categories of individuals, devices and activities that are more susceptible to improvements. By addressing the multidimensional factors influencing the mechanisms of tDCS, we also provide suggestions for future research.

Flow-mediated dilation is modified by exercise training status during childhood and adolescence: preliminary evidence of the youth athlete's artery.

Chronic exercise training is associated with an "athlete's artery" phenotype in young adults and an attenuated age-related decline in endothelium-dependent arterial function. Adolescence is associated with an influx of sex-specific hormones that may exert divergent effects on endothelial function, but whether training adaptations interact with biological maturation to produce a "youth athlete's artery" has not been explored. We investigated the influence of exercise-training status on endothelium-dependent arterial function during childhood and adolescence. Brachial artery flow-mediated dilation (FMD) was assessed in n = 102 exercise-trained (males, n = 25; females, n = 29) and untrained (males, n = 23; females, n = 25) youths, characterized as pre (males, n = 25; females, n = 26)- or post (males, n = 23; females, n = 28)-predicted age at peak height velocity (PHV). Baseline brachial artery diameter was larger in post- compared with pre-PHV youths (P ≤ 0.001), males compared with females (P ≤ 0.001), and trained compared with untrained youths (3.26 ± 0.51 vs. 3.11 ± 0.42 mm; P = 0.041). Brachial FMD was similar in pre- and post-PHV youths (P = 0.298), and males and females (P = 0.946). However, exercise-trained youths demonstrated higher FMD when compared with untrained counterparts (5.3 ± 3.3 vs. 3.0 ± 2.6%; P ≤ 0.001). Furthermore, brachial artery diameter (r2 = 0.142; P = 0.007 vs. r2 = 0.004; P = 0.652) and FMD (r2 = 0.138; P = 0.008 vs. r2 = 0.003; P = 0.706) were positively associated with cardiorespiratory fitness in post-, but not pre-PHV youths, respectively. Collectively, our data indicate that exercise training is associated with brachial artery remodeling and enhanced endothelial function during youth. However, arterial remodeling and endothelium-dependent function are only associated with elevated cardiorespiratory fitness during later stages of adolescence.NEW & NOTEWORTHY We report preliminary evidence of the "youth athlete's artery," characterized by training-related arterial remodeling and elevated endothelium-dependent arterial function in children and adolescents. However, training-related adaptations in brachial artery diameter and flow-mediated dilation (FMD) were associated with cardiorespiratory fitness in adolescents, but not in children. Our findings indicate that endothelium-dependent arterial function is modifiable with chronic exercise training during childhood, but the association between FMD and elevated cardiorespiratory fitness is only apparent during later stages of adolescence.

Abstract P184: Association of Plasma Lipid Species With Lipoprotein Insulin Resistance Score Before and After Exercise Training

Background: The lipoprotein insulin resistance (LP-IR) score has been shown to assess insulin resistance, predict future type 2 diabetes, and improve with regular exercise. The lipidomic profile is known to differ with insulin resistance and type 2 diabetes, but few studies have examined lipidome associations with LP-IR, particularly in response to an exercise intervention. Methods: Plasma lipids were measured using the C8-positive LC-MS method in 671 participants from the HERITAGE Family Study (56% Female, 35% Black, 35.2 yrs) before and after a 20-week exercise intervention. LP-IR, a weighted index of six lipoprotein parameters, was measured before and after training through nuclear magnetic resonance spectroscopy (Labcorp, NC). Linear mixed models were used to test the associations of 193 known plasma lipids with LP-IR before and after exercise training. All models were adjusted for age, sex, and race, while post-training models also adjusted for baseline lipid and baseline LP-IR. Results: A total of 162 lipids (84%) were associated (FDR&lt;0.05) with LP-IR at baseline ( Figure 1 ). The top positive associations were found for TG species, while cholesterol ester species showed the top inverse associations. Following training, the change in 143 (74%) lipids were associated (FDR&lt;0.05) with change in LP-IR. There were 129 lipid species associated with both baseline and changes in LP-IR, while 33 were only associated with baseline measures and 14 only associated with changes in LP-IR following exercise ( Figure 1 ). Conclusions: We found that most lipids were associated with LP-IR regardless of exercise training status. Importantly, we identified a subset of lipids that were only associated with changes in LP-IR, which may represent exercise responsive biomarkers of exercise induced changes in LP-IR. However, further research is needed to identify the biological mechanisms connecting the changes in these lipid species and metabolic changes following exercise training.

Acute oral antioxidant consumption does not alter brachial artery flow mediated dilation in young adults independent of exercise training status.

Endothelium-dependent vasodilation can be tested using a variety of shear stress paradigms, some of which may involve the production of reactive oxygen species. The purpose of this study was to compare different methods for assessing endothelial function and their specific involvement of reactive oxygen species and influence of aerobic training status. Twenty-nine (10F) young and healthy participants (VO2max: 34-74mL·kg-1·min-1) consumed either an antioxidant cocktail (AOC; vitamin C, vitamin E, α-lipoic acid) or placebo (PLA) on each of two randomized visits. Endothelial function was measured via three different brachial artery flow-mediated dilation (FMD) tests: reactive hyperemia (RH-FMD: 5min cuff occlusion and release), sustained shear (SS-FMD: 6 min rhythmic handgrip), and progressive sustained shear (P-SS-FMD: three intensities of 3 min of rhythmic handgrip). Baseline artery diameter decreased (all tests: 3.8±0.5 to 3.7±0.6mm, p=0.004), and shear rate stimulus increased (during RH-FMD test, p=0.021; during SS-FMD test, p=0.36; during P-SS-FMD test, p=0.046) following antioxidant consumption. However, there was no difference in FMD following AOC consumption (RH-FMD, PLA: 8.1±2.6%, AOC: 8.2±3.5%, p=0.92; SS-FMD, PLA: 6.9±3.9%, AOC: 7.8±5.2%, p=0.15) or FMD per shear rate slope (P-SS-FMD: PLA: 0.0039±0.0035mm·s-1, AOC: 0.0032±0.0017mm·s-1, p=0.28) and this was not influenced by training status/fitness (all p>0.60). Allometric scaling did not alter these outcomes (all p>0.40). Reactive oxygen species may not be integral to endothelium-dependent vasodilation tested using reactive, sustained, or progressive shear protocols in young males and females, regardless of fitness level.

Cerebral blood flow and cerebrovascular reactivity are modified by maturational stage and exercise training status during youth.

What is the central question of this study? Gonadal hormones modulate cerebrovascular function while insulin-like growth factor 1 (IGF-1) facilitates exercise-mediated cerebral angiogenesis; puberty is a critical period of neurodevelopment alongside elevated gonadal hormone and IGF-1 activity: but whether exercise training across puberty enhances cerebrovascular function is unkown. What is the main finding and its importance? Cerebral blood flow is elevated in endurance trained adolescent males when compared to untrained counterparts. However, cerebrovascular reactivity to hypercapnia is faster in trained vs. untrained children, but not adolescents. Exercise-induced improvements in cerebrovascular function are attainable as early as the first decade of life. Global cerebral blood flow (gCBF) and cerebrovascular reactivity to hypercapnia ( ) are modulated by gonadal hormone activity, while insulin-like growth factor 1 facilitates exercise-mediated cerebral angiogenesis in adults. Whether critical periods of heightened hormonal and neural development during puberty represent an opportunity to further enhance gCBF and is currently unknown. Therefore, we used duplex ultrasound to assess gCBF and in n=128 adolescents characterised as endurance-exercise trained (males: n=30, females: n=36) or untrained (males: n=29, females: n=33). Participants were further categorised as pre- (males: n=35, females: n=33) or post- (males: n=24, females: n=36) peak height velocity (PHV) to determine pubertal or 'maturity' status. Three-factor ANOVA was used to identify main and interaction effects of maturity status, biological sex and training status on gCBF and . Data are reported as group means (SD). Pre-PHV youth demonstrated elevated gCBF and slower mean response times than post-PHV counterparts (both: P≤0.001). gCBF was only elevated in post-PHV trained males when compared to untrained counterparts (634(43) vs. 578(46)mlmin-1 ; P=0.007). However, mean response time was faster in pre- (72(20) vs. 95(29)s; P≤0.001), but not post-PHV (P=0.721) trained youth when compared to untrained counterparts. Cardiorespiratory fitness was associated with gCBF in post-PHV youth (r2 =0.19; P≤0.001) and mean response time in pre-PHV youth (r2 =0.13; P=0.014). Higher cardiorespiratory fitness during adolescence can elevate gCBF while exercise training during childhood primes the development of cerebrovascular function, highlighting the importance of exercise training during the early stages of life in shaping the cerebrovascular phenotype.

The effects of exercise training on insulin resistance in children and adolescents with overweight or obesity: a systematic review and meta-analysis.

The aim of present meta-analysis was to determine the effects of exercise training (Exe) on insulin resistance (IR) and body weight in children and adolescents with overweight or obesity. PubMed, Web of Science, and Scopus were searched for original articles, published through October 2022 that included exercise versus control interventions on fasting glucose, insulin, HOMA-IR, and body weight outcomes in children and adolescents with overweight or obesity. Standardized mean differences (SMD) for fasting insulin, and weighted mean differences (WMD) for fasting glucose, HOMA-IR, body weight (BW), and 95% confidence intervals were determined using random effects models. Thirty-five studies comprising 1,550 children and adolescents with overweight and obesity were included in the present meta-analysis. Exercise training reduced fasting glucose (WMD=-2.52 mg/dL, p=0.001), fasting insulin (SMD=-0.77, p=0.001), HOMA-IR (WMD=-0.82, p=0.001), and BW (WMD=-1.51 kg, p=0.001), as compared to a control. Subgroup analyses showed that biological sex, intervention duration, type of exercise training, BMI percentile, and health status (with or without diagnosed condition), were sources of heterogeneity. Exercise training is effective for lowering fasting glucose, fasting insulin, HOMA-IR, and BW in children and adolescents with overweight or obesity and could provide an important strategy for controlling IR and related factors. With clear evidence for the effectiveness of exercise interventions in this vulnerable population, it is important to determine effective approaches for increasing exercise training in children and adolescents with overweight or obesity.

NEUROVASCULAR COUPLING AND CEREBROVASCULAR HAEMODYNAIMCS ARE MODIFIED BY EXERCISE TRAINING STATUS AT DIFFERENT STAGES OF MATURATION DURING YOUTH.

Neurovascular coupling (NVC) is mediated via nitric oxide signalling, which is independently influenced by sex hormones and exercise training. Whether exercise training differentially modifies NVC pre- vs. post-puberty, where levels of circulating sex hormones will differ greatly within- and between-sexes, remains to be determined. Therefore, we investigated the influence of exercise training-status on resting intra-cranial haemodynamics and NVC at different stages of maturation. Posterior and middle cerebral artery velocities (PCAv and MCAv) and pulsatility index (PCAPI and MCAPI) were assessed via trans-cranial Doppler ultrasound at rest and during visual NVC stimuli. N=121 exercise-trained (males: n=32, females: n=32) and untrained (males: n=28, females: n=29) participants were characterised as pre- (males: n=33, females: n=29) or post- (males: n=27, females: n=32) peak height velocity (PHV). Exercise-trained youth demonstrated higher resting MCAv (P=0.010). Maturity- and training-status did not affect the ∆PCAv and ∆MCAv during NVC. However, pre-PHV untrained males (19.4±13.5 vs. 6.8±6.0%; P≤0.001) and females (19.3±10.8 vs. 6.4±7.1%; P≤0.001) had a higher ∆PCAPI during NVC than post-PHV untrained counterparts, while the ∆PCAPI was similar in pre- and post-PHV trained youth. Pre-PHV untrained males (19.4±13.5 vs. 7.9±6.0%; P≤0.001) and females (19.3±10.8 vs 11.1±7.3%; P=0.016) also had a larger ∆PCAPI than their pre-PHV trained counterparts during NVC, but the ∆PCAPI was similar in trained and untrained post-PHV youth. Collectively, our data indicate that exercise training elevates regional cerebral blood velocities during youth, but training-mediated adaptations in NVC are only attainable during early stages of adolescence. Therefore, childhood provides a unique opportunity for exercise-mediated adaptations in NVC.

Physical capacity increase in patients with heart failure is associated with improvement in muscle sympathetic nerve activity

BackgroundExercise training improves physical capacity in patients with heart failure with reduced ejection fraction (HFrEF), but the mechanisms involved in this response is not fully understood. The aim of this study was to determine if physical capacity increase in patients HFrEF is associated with muscle sympathetic nerve activity (MSNA) reduction and muscle blood flow (MBF) increase. MethodsThe study included 124 patients from a 17-year database, divided according to exercise training status: 1) exercise-trained (ET, n = 83) and 2) untrained (UNT, n = 41). MSNA and MBF were obtained using microneurography and venous occlusion plethysmography, respectively. Physical capacity was evaluated by cardiopulmonary exercise test. Moderate aerobic exercise was performed 3 times/wk. for 4 months. ResultsExercise training increased peak oxygen consumption (V̇O2, 16.1 ± 0.4 vs 18.9 ± 0.5 mL·kg−1·min−1, P < 0.001), LVEF (28 ± 1 vs 30 ± 1%, P = 0.027), MBF (1.57 ± 0.06 vs 2.05 ± 0.09 mL.min−1.100 ml−1, P < 0.001) and muscle vascular conductance (MVC, 1.82 ± 0.07 vs 2.45 ± 0.11 units, P < 0.001). Exercise training significantly decreased MSNA (45 ± 1 vs 32 ± 1 bursts/min, P < 0.001). The logistic regression analyses showed that MSNA [(OR) 0.921, 95% CI 0.883–0.962, P < 0.001] was independently associated with peak V̇O2. ConclusionsThe increase in physical capacity provoked by aerobic exercise in patients with HFrEF is associated with the improvement in MSNA.

Ischemic preconditioning and exercise performance: are the psychophysiological responses underestimated?

The findings of the ischemic preconditioning (IPC) on exercise performance are mixed regarding types of exercise, protocols and participants' training status. Additionally, studies comparing IPC with sham (i.e., low-pressure cuff) and/or control (i.e., no cuff) interventions are contentious. While studies comparing IPC versus a control group generally show an IPC significant effect on performance, sham interventions show the same performance improvement. Thus, the controversy over IPC ergogenic effect may be due to limited discussion on the psychophysiological mechanisms underlying cuff maneuvers. Psychophysiology is the study of the interrelationships between mind, body and behavior, and mental processes are the result of the architecture of the nervous system and voluntary exercise is a behavior controlled by the central command modulated by sensory inputs. Therefore, this narrative review aims to associate potential IPC-induced positive effects on performance with sensorimotor pathways (e.g., sham influencing bidirectional body-brain integration), hemodynamic and metabolic changes (i.e., blood flow occlusion reperfusion cycles). Overall, IPC and sham-induced mechanisms on exercise performance may be due to a bidirectional body-brain integration of muscle sensory feedback to the central command resulting in delayed time to exhaustion, alterations on perceptions and behavior. Additionally, hemodynamic responses and higher muscle oxygen extraction may justify the benefits of IPC on muscle contractile function.

Prenatal Exercise and Cardiovascular Health (PEACH) Study: impact of pregnancy and exercise on rating of perceived exertion during non-weight-bearing exercise.

The purpose of this study was to determine if rating of perceived exertion (RPE) during non-weight-bearing exercise is influenced by gestational age and exercise training. We conducted a randomized controlled trial to examine the influence of gestational age and exercise training (three to fourtimes per week for 25-40 minutes at 50%-70% of heart rate reserve) on RPE during an exhaustive cycling exercise test. We observed no influence of gestational age, or exercise training status on RPE responses to non-weight-bearing exercise during pregnancy. Trial registration number: NCT02948439. Novelty: Gestational age and/or exercise training does not influence rate of perceived exertion during non-weight-bearing exercise.

Mitochondrial Mass of Naïve T Cells Is Associated with Aerobic Fitness and Energy Expenditure of Active and Inactive Adults.

Chronic exercise training is known to induce metabolic changes, but whether these adaptations extend to lymphocytes and how this may affect immune function remains largely unknown. This study was conducted to determine the extent to which mitochondrial characteristics of naïve T cells differ according to fitness status and to further examine the energy production pathways of cells from aerobically trained and inactive participants. Blood was collected from 30 aerobically active (>6 h·wk -1 ) or inactive (<90 min·wk -1 ) men and women. Naïve T cell mitochondrial mass, membrane potential, and biogenesis were assessed with flow cytometry. Participants completed a treadmill maximal oxygen consumption (V̇O 2peak ) test and wore a physical activity monitor for 1 wk. In a subset of participants, naïve CD8 + T cell activation-induced glycolytic and mitochondrial ATP production was measured. Active participants exhibited 16.7% more naïve CD8 + T cell mitochondrial mass ( P = 0.046), 34% greater daily energy expenditure ( P < 0.001), and 39.6% higher relative V̇O 2peak ( P < 0.001), along with 33.9% lower relative body fatness ( P < 0.001). Among all participants, naïve CD8 + T cell mitochondrial mass was correlated with estimated energy expenditure ( r = 0.36, P = 0.048) and V̇O 2peak ( r = 0.47, P = 0.009). There were no significant differences in ATP production, mitochondrial biogenesis, or mitochondrial membrane potential between active and inactive groups. This is the first study to examine the effects of aerobic exercise training status on metabolic parameters within human naïve T cells. Findings suggest that mitochondrial adaptations in certain immune cell types are positively associated with aerobic fitness and energy expenditure. This study provides a foundation for future development of prophylactic and therapeutic interventions targeting specific immune cell subsets to improve the immune response and overall health.

유산소운동 중 최대 지방 산화에 관한 고찰

This study proposed a method for measuring the maximum fat oxidation rate along with a review of maximum fat oxidation (MFO) during aerobic exercise. In addition, we discussed the specific research results that each factor, such as training, sex, and dietary status, promotes or inhibits physiological changes in fat oxidation as determinants of MFO and Fatmax during exercise. As a research method, in order to examine the measurement of fatty acid oxidation and maximum fat oxidation rate during exercise, a literature search was conducted to identify all studies using the Fatmax protocol in the adult population. In the literature, ‘aerobic exercise’, ‘fat oxidation’, ‘maximum fat oxidation’, and ‘Fatmax’ were searched in PubMed and Web of Science databases, Korea Education and Research Information Service (RISS), and 76 studies were included in the review. Exercise intensity, duration, training status, gender and availability of dietary nutrients are all determinants of MFO and Fatmax, all of which affect systemic fat oxidation. In particular, exercise intensity has a significant effect on fat oxidation regardless of training status and dietary effects. Nevertheless, exercise regimens that lead to the ideal adaptation of fat oxidation related to improving exercise effects should be considered.

Physical fitness status modulates the inflammatory proteins in peripheral blood and circulating monocytes: role of PPAR-gamma

The aim of this study was to analyze the metabolic and molecular profile according to physical fitness status (Low or High VO2max) and its impacts on peripheral and cellular inflammatory responses in healthy men. First (Phase I), inflammatory profile (TNF-α, IL-6, IL-10) was analyzed at baseline and post-acute exercise sessions performed at low (< 60% VO2max) and high (> 90% VO2max) intensities considering the individual endotoxin concentrations. Next (Phase II), monocyte cell cultures were treated with LPS alone or associated with Rosiglitazone (PPAR-γ agonist drug) to analyze cytokine production and gene expression. Monocyte subsets were also evaluated by flow cytometry. A positive relationship was observed between LPS concentrations and oxygen uptake (VO2max) (r = 0.368; p = 0.007); however, in the post-exercise an inverse correlation was found between LPS variation (Δ%) and VO2max (r = -0.385; p = 0.004). With the low-intensity exercise session, there was inverse correlation between LPS and IL-6 concentrations post-exercise (r = -0.505; p = 0.046) and a positive correlation with IL-10 in the recovery (1 h post) (r = 0.567; p = 0.011), whereas with the high-intensity exercise an inverse correlation was observed with IL-6 at pre-exercise (r = -0.621; p = 0.013) and recovery (r = -0.574; p = 0.016). When monocyte cells were treated with LPS, High VO2max individuals showed higher PPAR-γ gene expression whereas Low VO2max individuals displayed higher IL-10 production. Additionally, higher TLR-4, IKK1, and PGC-1α gene expression were observed in the High VO2max group than Low VO2max individuals. In conclusion, even with elevated endotoxemia, individuals with High VO2max exhibited higher IL-6 concentration in peripheral blood post-acute aerobic exercise and lower IL-10 concentration during recovery (1 h post-exercise). The anti-inflammatory effects linked with exercise training and physical fitness status may be explained by a greater gene expression of IKK1, TLR-4, and PGC-1α, displaying an extremely efficient cellular framework for the PPAR-γ responses.

Long-term athletic training does not alter age-associated reductions of left-ventricular mid-diastolic lengthening or expansion at rest

PurposeThe interaction of ageing and exercise training status on left-ventricular (LV) peak strain is unclear. Additionally, strain analysis across the entire cardiac cycle facilitates a more detailed assessment of deformation, yet this has not been implemented to characterize the ageing LV and in association with training status. This study investigated healthy ageing and training status on LV systolic and diastolic strain utilizing novel echocardiographic applications.MethodsForty healthy males were included and allocated into four groups; young recreationally active (YRA,n = 9; 28 ± 5 years), old recreationally active (ORA, n = 10; 68 ± 6), young trained (YT,n = 10; 27 ± 6 years), and old trained (OT, n = 11, 64 ± 4 years) groups. Two-dimensional speckle-tracking echocardiography was performed to ascertain peak LV longitudinal and circumferential strain (base and apex) strain within each myocardial layer and at 5% increments across the cardiac cycle.ResultsOlder groups had lower diastolic longitudinal lengthening and circumferential expansion between 40–85% mid-diastole, regardless of training status (P < 0.05). Whereas, strain throughout systole was similar between groups (P > 0.05). Longitudinal and circumferential (base and apex) peak and layer-specific strain did not differ between groups (P > 0.05).ConclusionNovel applications of diastolic strain revealed lower age-associated LV longitudinal lengthening and circumferential expansion in older age. Yet, diastolic strain profiles did not differ based on chronic habits of exercise training and, thus, older trained men did not demonstrate an attenuation of age-associated differences in mid-diastolic LV strain.

Diietary Fat Retention In Cortical Bone In Rats Fed High Fat Diet

Excess adiposity during childhood and adolescence are associated with poorer bone quality and increased risk of fracture. A high fat diet (HFD) may promote marrow adipogenesis at the expense of osteogenesis and may also promote excess lipid accumulation in osteoblasts and osteocytes. Exercise is prescribed for bone health primarily because of its mechanical loading attributes, but little is known about how obesity or exercise training status affects nutrient trafficking and retention in bone. PURPOSE: To determine the effects of obesity and exercise on fat retention in bone. METHODS: Female Wistar rats, 5 weeks in age (n=18), were fed a high fat diet (HFD) for 20 weeks and designated as obese (OB) or lean (LN) based on weight gain. OB and LN rats performed treadmill running (EX) (5 d/w, 1h/d, 15 m/min) or sedentary control (SED) for 10 weeks. In a 24-hour tracer study, rats were given ad libitum access to 14C oleate and 14C palmitate blended in food. Marrow was removed from hind limb bones and lipid was extracted from the remaining cortical bone. Data are mean ± SEM. RESULTS: Fat intake was not different between groups. Obesity status had no significant effect on fat retention in cortical bone. Lipid in hindlimb cortical bone was higher in EX compared to SED (EX: 6.66 ± 1.64 vs SED: 1.50 ± 1.47 mg, p<0.05). LNEX had the highest dietary fat retention in cortical bone lipid compared to other groups (LNEX: 0.69 ± 0.14, OBEX: 0.24 ± 0.14, LNSED: 0.27 ± 0.13, OBSED: 0.41 ± 0.13 mg, interaction p<0.05). The proportion of the meal retained, and the magnitude of lipid turnover did not differ between groups. Dietary fat retention in bone was positively (p<0.05) associated with fat intake, bone mineral density and bone mineral content (BMC; r=0.51-0.64). BMC in bone was associated (r=0.54, p<0.05) with total energy expenditure. CONCLUSION: Although cortical bone constitutes a minor fraction of overall dietary fat retention, we demonstrate that fat is trafficked to and retained in cortical bone in proportion to meal size and bone size. Future work is needed to test whether fat accumulation influences the ability to adapt to mechanical loading.

Impact of exercise training status on the fiber type-specific abundance of proteins regulating intramuscular lipid metabolism.

Endurance training enhances the capacity for fat oxidation during exercise due to increased utilization of intramuscular lipid (IMCL). This study quantitatively investigated the impact of exercise training status on muscle fiber type-specific abundance of regulatory proteins involved in IMCL utilization. Endurance-trained [n = 7 subjects, peak oxygen consumption (V̇o2peak) 62.6 ± 4.1 (SD) mL·min-1·kg-1] and non-endurance-trained (n = 8 subjects, V̇o2peak 44.9 ± 5.3 mL·min-1·kg-1) young men completed an incremental exercise test to determine maximal fat oxidation (MFO) and maximal oxygen uptake. Fiber type-specific IMCL content and protein abundance were assessed with immunofluorescence microscopy and immunoblot analysis of pooled single muscle fibers and whole muscle. Endurance-trained individuals displayed a higher MFO rate (0.45 ± 0.15 vs. 0.19 ± 0.07 g/min, P < 0.05), a greater proportion of type I muscle fibers, and higher IMCL content compared with untrained individuals (P < 0.05). Adipose triglyceride lipase, hormone-sensitive lipase, perilipin 2, perilipin 5, and hydroxyacyl-coenzyme A dehydrogenase abundances were ~2-3-fold higher in type I muscle fibers compared with type IIa fibers (P < 0.05). Correspondingly, these lipid proteins and oxidative enzymes were higher in endurance-trained individuals when assessed in whole muscle. MFO rate was strongly related to the proportion of type I fibers (R = 0.81, P < 0.01). The abundance of proteins involved in the regulation of IMCL storage and oxidation is highly muscle fiber type specific. The increased capacity for fat oxidation in endurance-trained individuals corresponded with increased IMCL content and elevated abundance of lipolytic and oxidative enzymes in combination with a greater proportion of type I muscle fibers.NEW & NOTEWORTHY We have utilized contemporary techniques to compare the fiber type-specific characteristics of skeletal muscle from endurance-trained athletes and untrained individuals. We show that type I muscle fibers have a coordinated upregulation of proteins controlling intramuscular lipid storage, mobilization, and oxidation. Furthermore, the enhanced capacity for intramuscular lipid storage and utilization in endurance-trained individuals is related to the increased expression of lipid regulatory proteins combined with a greater proportion of type I muscle fibers.

Circulating Fatty Acid Binding Protein 4 Concentration Increases with an Acute Maximal Exercise Independently of Exercise Training Status

Circulating Fatty Acid Binding Protein 4 Concentration Increases with an Acute Maximal Exercise Independently of Exercise Training Status