Excess Phosphate Research Articles

Effects of Culture Conditions on the Performance of Arthrospira platensis and Its Production of Exopolysaccharides.

Exopolysaccharides (EPS) produced by Arthrospira platensis (A. platensis) has been widely applied in industry and commerce for its various activities but the accumulation of EPS in culture medium may influence the growth of A. platensis reversely. This work aims to explore the impacts of initial pH, nitrogen source and concentration, phosphate concentration and recycle times of the culture medium on the growth of A. platensis and the secretion of its EPS. The results showed that EPS accumulated with the increase in recycle times of culture medium. The optimal initial pH for the growth of A. platensis was 8.50, and high pH of 11.5 inhibited the growth of biomass while resulting in highest EPS content of 92.87 mg/g DW. Excessive and limited nitrogen (NaNO3 of 25.00 g/L and NaNO3 < 2.50 g/L) and phosphate (K2HPO4 of 5.00 g/L and K2HPO4 < 0.50 g/L) inhibited the biomass production of A. platensis by 1.28–30.77% and 14.29–45.05%, respectively. EPS yield of 97.57 mg/g DW and 40.90 mg/g DW were obtained under NaNO3 of 25.00 g/L and K2HPO4 of 5.00 g/L due to salt stress. These findings are beneficial in providing a theoretical basis for high yield EPS from A. platensis without affecting biomass yield.

Biological effect of phosphate on the dissimilatory arsenate-respiring bacteria-catalyzed reductive mobilization of arsenic from contaminated soils

Dissimilatory arsenate-respiring prokaryotes (DARPs) are considered to be the major drive of the reductive mobilization of arsenic from solid phases. However, it is not fully understood how phosphate, a structural analog of arsenate, affects the DARPs-mediated arsenic mobilization. This work aimed to address this issue. As-contaminated soils were collected from a Shimen Realgar Mine-affected area. We identified a unique diversity of DARPs from the soils, which possess high As(V)-respiring activities using one of multiple small organic acids as the electron donor. After elimination of the desorption effect of phosphate on the As mobilization, the supplement of additional 10 mM phosphate to the active slurries markedly increased the microbial community-mediated reductive mobilization of arsenic as revealed by microcosm tests; this observation was associated to the fact that phosphate significantly increased the As(V)-respiratory reductase (Arr) gene abundances in the slurries. To confirm this finding, we further obtained a new DARP strain, Priestia sp. F01, from the samples. We found that after elimination of the chemical effect of phosphate, the supplement of 10 mM phosphate to the active slurries resulted in an 82.2% increase of the released As(III) in the solutions, which could be contributed to that excessive phosphate greatly increased the Arr gene abundance, and enhanced the transcriptional level of arrA gene and the bacterial As(V)-respiring activity of F01 cells. Considering that phosphate commonly coexists with As in the environment, and is a frequently-used fertilizer, these findings are helpful for deeply understanding why As concentrations in contaminated groundwater are dynamically fluctuated, and also provided new knowledge on the interactions between the biogeochemical processes of P and As.

Enhanced Ultrasensitive Photoelectrochemical Probe for Phosphate Detection in Water Based on a Zirconium-Porphyrin Framework.

Excessive phosphate poses a serious ecological and human health risk, and thereby, monitoring its trace concentration is of great significance to environmental protection and human health. In this work, a zirconium-porphyrin framework (PCN-222) with excellent stability and unique luminescence properties was designed to modify the surface of the indium tin oxide electrode, which was first used as a photoelectrochemical (PEC) probe for phosphate detection. The PCN-222-modified PEC probe demonstrated an excellent selectivity and stability and indicated a linear response to phosphate in the range of 0-106 nM with a limit of detection (LOD) as low as 1.964 nM. To the best of our knowledge, this is the phosphate probe with the lowest LOD, and this is also the first signal-on PEC probe toward phosphate based on PCN-222. More importantly, the PEC probe can be validated for the good applicability of trace phosphate detection in real water samples, indicating a good application prospect. Finally, a series of electrochemical and spectroscopic studies have proved that phosphate can bind to the indium tin oxide (ITO)/PCN-222 electrode, which shortens the distance of the space charge region while reducing the bandwidth and thus facilitates the transfer of photogenerated electrons across the energy band barrier to reduce O2 in the electrolyte, producing an enhanced cathodic photocurrent signal. The proposed strategy of the highly sensitive PEC probe provides a promising platform for more effective label-free phosphate monitoring in the environment and organisms.

Phosphate Treatment by Five Onsite Wastewater Systems in a Nutrient Sensitive Watershed

Excess phosphate (P) loading to surface waters increases productivity sometimes causing an imbalance leading to eutrophication and water quality degradation. Wastewater contains elevated concentrations of P and other contaminants that pose threats to environmental health. Onsite wastewater systems (OWS) are used in many rural areas, but most are not monitored for P removal effectiveness. The goal of this research was to gain a better understanding of the P treatment efficiency of OWS in a nutrient-sensitive watershed. Groundwater monitoring infrastructure, including networks of wells and piezometers, was installed at five sites with OWS in coastal North Carolina. Groundwater samples from the piezometers and wastewater samples from the septic tanks were collected and analyzed for phosphate and physicochemical parameters. Results suggest that significant reductions in P concentrations (79.7 to 99.1%) were occurring in the soil immediately beneath the drainfield trenches, but P concentrations in groundwater near the OWS and more than 35 downgradient were alike and significantly elevated relative to background concentrations. OWS in areas with sandy soils such as the Hoods Creek watershed may be sources of P to groundwater and surface water. Nutrient management policies in watersheds with sandy soils should include provisions for assessing and reducing P contributions from OWS to surface waters.

Wastewater Purification from Excess Phosphates Using Bentonite Activated by Microwave Radiation

Wastewater Purification from Excess Phosphates Using Bentonite Activated by Microwave Radiation

Study of water quality, socio-economic status and policy intervention in spring ecosystems of Tripura, Northeast India

Spring is vital in all hilly areas. Without question, springs have aided in the advancement of human civilization. Mountain springs supply water to rural families in the Northeast. This spring ecological study was conducted in Dhalai, Tripura, with socio-economic policy significance. The springs chosen were Jamircherra (JS) and Govindabari (GS). The seasonal features of each spring were studied. The monsoon season is used to bring the life-giving flow of perennial springs. Several water quality indicators like WT, pH, EC, TDS, Turb, TH, DO, BOD, Ca+2, Mg+2, Cl−, No3, Po4 were examined to assess the risk of spring contamination. The most common aberrant results are samples having excessive phosphate (PO42−) and turbidity levels compared to norms. The spring's water quality was tested using the weighted arithmetic index methodology. The water quality at the two springs was adequate but not great throughout the year, causing human deaths from water-borne diseases. Thus, policy implementation was emphasized to save the spring and human life. A physicochemical evaluation of both springs was used to describe a techno-legal component of Environmental regulations.

Association of Serum Phosphate with Efficacy of Statin Therapy in Hemodialysis Patients.

Statins are less efficacious in reducing cardiovascular disease risk in patients on dialysis than in the general population. Recent experimental data showed that phosphate excess promotes cellular de novo cholesterol synthesis through 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase activation. Whether this mechanism might account for the resistance of patients on dialysis to statins has not yet been explored. In this post hoc analysis, we examined the efficacy of statin treatment according to serum phosphate levels in the patients on dialysis who were participants of the A Study to Evaluate the Use of Rosuvastatin in Subjects on Regular Hemodialysis: An Assessment of Survival and Cardiovascular Events (AURORA) trial using serum phosphate levels at baseline and during the trial course. We first classified the patients by groups of similar phosphate trajectories over time and tested whether phosphate as a longitudinal exposure (summarized by the identified trajectory groups) modulated the occurrence of major adverse cardiovascular events and all-cause death. We replicate the analysis in the Deutsche Diabetes Dialyze Studie (4D) trial. In the AURORA trial, using multivariable analysis, we found that the treatment effect of statin on major adverse cardiovascular events and all-cause death was significant and protective effects in patients with low values of serum phosphate gradually faded for higher phosphate levels >5 mg/dl. A similar lack of statin treatment efficacy for both outcomes was observed with high baseline phosphate levels (>5 mg/dl). In the 4D trial, we found a comparable but not significant trend toward losing treatment efficacy in the presence of high serum phosphate levels for both outcomes. Our results demonstrated the limited treatment efficacy of statins in patients on dialysis in the presence of hyperphosphatemia.

Dual-active-sites oriented mesoporous zirconia nanofibers for enhanced removal of phosphate in the presence of humic acid

Excessive phosphate in water will inevitably cause eutrophication and further trigger major environmental issues. Efficient removal of phosphate is of great significance to inhibit water eutrophication, however, humic acid (HA) in water aggravates the uncertainty of phosphate removal. In this work, mesoporous zirconia nanofiber (MZN) was synthesized and applied as an adsorbent for phosphate removal in the presence of HA. The performance and mechanism of the enhanced removal of phosphate by MZN in the presence of HA was systematically studied. The results show that MZN has higher adsorption capacity, stronger affinity, and faster adsorption rate for phosphate in solution with HA than that without HA. In the presence of HA, HA is adsorbed on MZN through complexation, which introduces carboxyl and hydroxyl groups to MZN surface, increasing phosphate adsorption through hydrogen bonding. The boosted removal mechanism of phosphate can be ascribed to the dual-active-sites effect between the complexation of MZN to phosphate and the hydrogen bonding of the adsorbed HA to phosphate. The adsorption of phosphate by MZN in the presence of HA follows the outer-sphere complexation. Column adsorption experiment showed that MZN is feasible and effective for the treatment of phosphate in water containing HA based on the discharge standard of phosphate, and can be reused repeatedly through regeneration. The results indicate that MZN holds a great promise in efficient removal of phosphate in the presence of HA.

Synthesis and Characterization of 2D Magnesium Oxide Nanoflakes: A Potential Nanomaterial for Effective Phosphate Removal from Wastewater

AbstractPhosphate containing untreated wastewater, discharged into water bodies, can cause eutrophication, leading to harmful effects on the ecosystem and human health. This study focuses on a simple, low‐cost, and novel approach towards removing excess phosphate ions from aqueous solutions by adsorbing on onto two‐dimensional (2‐D) magnesium oxide nano‐flakes (MONFs). The phosphate removal efficiency was tested with different concentrations of phosphate solutions (2–25 mg L−1), pH levels (3–11), adsorbent dosages (0.025‐0.250 g), and contact times (10‐50 min). The maximum phosphate removal percentage of 100 % was obtained with 50.0 mL of 3 mg L−1 phosphate solution using 0.125 g of the synthesized MONFs with a phosphate absorption capacity of 29.9 mg g−1. Moreover, the adsorption data fit with the Freundlich isotherm, with an R2 value of 0.952. The adsorption kinetic data follow the pseudo‐second‐order model, with an R2 value of 0.995, suggesting that the adsorption process is chemisorption.

Effects of pharmacological inhibition of the sodium-dependent phosphate cotransporter 2b (NPT2b) on intestinal phosphate absorption in mouse and rat models.

An excess phosphate burden in renal disease has pathological consequences for bone, kidney, and heart. Therapies to decrease intestinal phosphate absorption have been used to address the problem, but with limited success. Here, we describe the in vivo effects of a novel potent inhibitor of the intestinal sodium‐dependent phosphate cotransporter NPT2b, LY3358966. Following treatment with LY3358966, phosphate uptake into plasma 15 min following an oral dose of radiolabeled phosphate was decreased 74% and 22% in mice and rats, respectively, indicating NPT2b plays a much more dominant role in mice than rats. Following the treatment with LY3358966 and radiolabeled phosphate, mouse feces were collected for 48 h to determine the ability of LY3358966 to inhibit phosphate absorption. Compared to vehicle‐treated animals, there was a significant increase in radiolabeled phosphate recovered in feces (8.6% of the dose, p < .0001). Similar studies performed in rats also increased phosphate recovered in feces (5.3% of the dose, p < .05). When used in combination with the phosphate binder sevelamer in rats, there was a further small, but not significant, increase in fecal phosphate. In conclusion, LY3358966 revealed a more prominent role for NPT2b on acute intestinal phosphate uptake into plasma in mice than rats. However, the modest effects on total intestinal phosphate absorption observed in mice and rats with LY3359866 when used alone or in combination with sevelamer highlights the challenge to identify new more effective therapeutic targets and/or drug combinations to treat the phosphate burden in patients with renal disease.

Aluminum-fumarate based MOF: A promising environmentally friendly adsorbent for the removal of phosphate

The removal of excessive phosphate from water is very important to avoid eutrophication. For this purpose, metal-organic frameworks (MOFs) have recently attracted great attention in phosphate adsorption due to their outstanding physicochemical features. However, most of the used MOFs in the adsorption of phosphate are based on those synthesized from organic ligands derived from petroleum sources such as tricarboxylic (BTC) and dicarboxylic acid (BDC), which can negatively impact the environment. Consequently, using stable MOF synthesized from an eco-friendly ligand is highly desired. In this work, Aluminum based fumarate MOF (Al-Fum) was used in the adsorption of phosphate from water and subsequently compared with other Al-MOF derived dicarboxylic acid (Al-BDC) and tricarboxylic acid (Al-BTC). The phosphate adsorption performance of the different synthesized Al-MOFs was evaluated with the help of different batch experiments related to the effect of adsorbent/adsorbate concentrations, the contact time, the pH and the temperature. Interestingly, Al-Fum displayed the highest adsorption capacity (67,62 mg P/g) compared to Al-BDC (47,58 mg P/g) and Al-BTC (23,17 mg P/g) at RT (22,2 °C). The regeneration of Al-Fum was tested for several cycles showing continuous adsorption capacity and stability. This work opens a new avenue toward the development and use of more environmentally friendly and reused MOFs for the removal of phosphate from water.

Obesity and Cancer: Potential Mediation by Dysregulated Dietary Phosphate

Next to smoking, obesity is the second leading preventable risk factor for cancer, but increasing rates of obesity and overweight are estimated to overtake smoking as the leading preventable cancer risk factor. Few research studies have investigated the dysregulated endocrine metabolism of dietary phosphate as a potential mediating factor in the association of obesity with cancer. Phosphate toxicity, the accumulation of excess phosphate in the body from dysregulated phosphate metabolism, is associated with tumorigenesis. High levels of hormones that regulate phosphate metabolism, such as parathyroid hormone and fibroblast growth factor 23, are also associated with obesity, providing a potential link between obesity and phosphate toxicity. Increased dietary intake of inorganic phosphate is linked to excessive consumption of foods processed with phosphate additives, and consumption of ultra-processed foods is associated with an increase in the incidence of obesity. Sugar-sweetened beverages provide the single largest source of sugar and energy intake in the U.S. population, and colas containing phosphoric acid are associated with tumorigenesis, suggesting another potential connection between obesity and cancer. Furthermore, dietary phosphate is positively correlated with increases in obesity, central obesity, and metabolic syndrome. The present perspective article proposes that dysregulated dietary phosphate potentially mediates the association of obesity with cancer.

A widely distributed phosphate-insensitive phosphatase presents a route for rapid organophosphorus remineralization in the biosphere

The regeneration of bioavailable phosphate from immobilized organophosphorus represents a key process in the global phosphorus cycle and is facilitated by enzymes known as phosphatases. Most bacteria possess at least one of three phosphatases with broad substrate specificity, known as PhoA, PhoX, and PhoD, whose activity is optimal under alkaline conditions. The production and activity of these phosphatases is repressed by phosphate availability. Therefore, they are only fully functional when bacteria experience phosphorus-limiting growth conditions. Here, we reveal a previously overlooked phosphate-insensitive phosphatase, PafA, prevalent in Bacteroidetes, which is highly abundant in nature and represents a major route for the regeneration of environmental phosphate. Using the enzyme from Flavobacterium johnsoniae, we show that PafA is highly active toward phosphomonoesters, is fully functional in the presence of excess phosphate, and is essential for growth on phosphorylated carbohydrates as a sole carbon source. These distinct properties of PafA may expand the metabolic niche of Bacteroidetes by enabling the utilization of abundant organophosphorus substrates as C and P sources, providing a competitive advantage when inhabiting zones of high microbial activity and nutrient demand. PafA, which is constitutively synthesized by soil and marine flavobacteria, rapidly remineralizes phosphomonoesters releasing bioavailable phosphate that can be acquired by neighboring cells. The pafA gene is highly diverse in plant rhizospheres and is abundant in the global ocean, where it is expressed independently of phosphate availability. PafA therefore represents an important enzyme in the context of global biogeochemical cycling and has potential applications in sustainable agriculture.

Excessive Inorganic Phosphate Burden Perturbed Intracellular Signaling: Quantitative Proteomics and Phosphoproteomics Analyses.

Inorganic phosphate (Pi) is an essential nutrient for the human body which exerts adverse health effects in excess and deficit. High Pi-mediated cytotoxicity has been shown to induce systemic organ damage, though the underlying molecular mechanisms are poorly understood. In this study, we employed proteomics and phosphoproteomics to analyze Pi-mediated changes in protein abundance and phosphorylation. Bioinformatic analyses and literature review revealed that the altered proteins and phosphorylation were enriched in signaling pathways and diverse biological processes. Western blot analysis confirms the extensive change in protein level and phosphorylation in key effectors that modulate pre-mRNA alternative splicing. Global proteome and phospho-profiling provide a bird-eye view of excessive Pi-rewired cell signaling networks, which deepens our understanding of the molecular mechanisms of phosphate toxicity.

Efficient phosphate capture of Fe3O4/UiO-66-NH2/CeO2 in wide pH spectrum

Eutrophication has long been one of the environmental pollution problems, and excess phosphate in water bodies is the main cause of this phenomenon. To solve this problem, magnetic adsorbent with Fe/Ce-loaded zirconium-based metal-organic framework (Fe3O4/UiO-66-NH2/CeO2) was prepared by co-precipitation, microwave assisted and hydrothermal method. The results showed that different molar ratios of cerium and zirconium were the main influencing factors for phosphate adsorption, and the adsorbent exerted its maximum capturing capacity of 260.8 mg/g of phosphate when Ce:Zr = 4:1. The newly synthesized Fe3O4/UiO-66-NH2/CeO2 was characterized by SEM, XRD, FT-IR, XPS, VSM and TGA. The adsorption process was found to be consistent with the Langmuir adsorption isotherm model and the pseudo-second-order model by batch experiments, indicating that the monolayer chemisorption dominated the adsorption process. Thermodynamic parameters obtained from temperature experiments proved that the phosphate adsorption process is a spontaneous endothermic absorption process and the adsorption experiments with coexisting ions (F−, Cl−, NO3−, SO42−, HCO3−) showed that the adsorbent has a unique selectivity for phosphate. Unlike most adsorbents previously studied, Fe3O4/UiO-66-NH2/CeO2 exhibited consistently efficient adsorption performance over the pH spectrum tested (2 < pH < 10). After five cycles, the adsorbent still maintained favourable adsorption performance. Mechanism of Fe3O4/UiO-66-NH2/CeO2 adsorption on phosphate was attributed to electrostatic attraction, ligand exchange and inner-sphere complexation. Therefore, the prepared Fe3O4/UiO-66-NH2/CeO2 with wide pH suitability will be a promising option for capturing phosphate from wastewater.

Phosphate Burden and Inflammation.

Phosphate is an essential macromineral often introduced to the body through dietary intake. The mechanisms for maintaining phosphate levels are tightly controlled via hormonal interactions and excretion via the kidneys. However, western diets consist of high levels of inorganic phosphate, which can overwhelm the regulatory mechanisms in place for maintaining homeostasis. Recent studies have found that phosphate burden can lead to activation of inflammatory signaling in various parts of the body. In addition, individuals with impaired kidney function may also experience exacerbated symptoms of phosphate overload due to decreased filtration and elimination. Many disease states can arise as a result of phosphate burden and subsequent inflammatory signaling, including cardiovascular diseases, tumorigenesis, depression, and neuronal disorders. While the pathophysiological causes of these diseases have been elucidated, there remains a need to address the clinical impacts of excessive dietary phosphate intake and to clarifypotential drug candidates that may help alleviate these conditions. This brief chapter looks to explain the overall connection between phosphate burden and inflammation in various diseases.

Vitamin D and Phosphate Interactions in Health and Disease.

Vitamin D plays an essential role in calcium and inorganic phosphate (Pi) homeostasis, maintaining their optimal levels to assure adequate bone mineralization. Vitamin D, as calcitriol (1,25(OH)2D), not only increases intestinal calcium and phosphate absorption but also facilitates their renal reabsorption, leading to elevated serum calcium and phosphate levels. The interaction of 1,25(OH)2D with its receptor (VDR) increases the efficiency of intestinal absorption of calcium to 30-40% and phosphate to nearly 80%. Serum phosphate levels can also influence 1,25(OH)2D and fibroblast growth factor 23 (FGF23) levels, i.e., higher phosphate concentrations suppress vitamin D activation and stimulate parathyroid hormone (PTH) release, while a high FGF23 serum level leads to reduced vitamin D synthesis. In the vitamin D-deficient state, the intestinal calcium absorption decreases and the secretion of PTH increases, which in turn causes the stimulation of 1,25(OH)2D production, resulting in excessive urinary phosphate loss. Maintenance of phosphate homeostasis is essential as hyperphosphatemia is a risk factor of cardiovascular calcification, chronic kidney diseases (CKD), and premature aging, while hypophosphatemia is usually associated with rickets and osteomalacia. This chapter elaborates on the possible interactions between vitamin D and phosphate in health and disease.

Low Inorganic Phosphate Stress Inhibits Liver Cancer Progression: from In Vivo to In Vitro

AbstractMetabolic stress (for example, low pO2, low pH) stimulates cancer progression in a complex and largely unresolved manner. Excessive inorganic phosphate burden is being considered as another stimulator, until now there is no well‐designed study to examine the potential benefits of reducing phosphate burden on cancer progression. Sevelamer microspheres, a polymeric phosphate binder, are introduced as embolic material for interventional treatment of rabbit VX2 liver cancer model. This technique is named as “transarterial sevelamer embolization (TASE).” The microspheres prove to be highly biocompatible, and TASE is found to be a safe local‐regional technique. Compared with conventional transarterial chemoembolization (TACE), TASE is found to not only occlude the tumor‐feeding vessel, but simultaneously deplete intratumoral inorganic phosphate (Pi), thereby inducing severe necrosis as well reducing metastasis and recurrence. Reduced Pi stress inhibits tumor vascularity, invasion, and metastasis by downregulating the angiogenic factors and oncoprotein expression. Energy metabolomics indicate that Pi stress also suppresses tumor anaerobic glycolysis and glutaminolysis. This systemic anti‐cancer effect indicates a promising new application for sevelamer and TASE as a potential alternative to conventional TACE for liver cancer treatment.

The Impact of Typhoon “Mangkhut” on Surface Water Nutrient and Chlorophyll Inventories of the South China Sea in September 2018

AbstractThe influence of the exceptionally strong typhoon Mangkhut on the availability of nutrients and changes in primary production were studied in the northern South China Sea in September 2018. A tight station grid was sampled to analyze major nutrients, chlorophyll_a, particulate and dissolved organic carbon and nitrogen. Based on interpolated profiles, nutrients and organic matter budgets were determined for the upper 100 m of the water column prior to and after Mangkhut's passage. An upper layer of 100 m was found to reflect the important changes by the typhoon. Considerable differences between the on‐shelf, shelf edge and the deep‐sea stations were determined. Nitrate and phosphate increased by about 80% and 36% on the shelf, respectively, and both by almost 40% at the shelf edge. The open deep‐sea part of the study area reflects some deviating results that may be caused by just displacement of water or by mixing water of different origin. However, right on Mangkhut's track on the shelf even contact between surface waters and bottom waters was enabled, increasing phosphate and silicate, but declining nitrate. The inventory of organic carbon of the upper 100 m of the study area (138,000 km2) of 92 Gmol had increased within a few days after the typhoon's passage by 5 Gmol on the shelf and about 2 Gmol in the shelf edge area. Chlorophyll_a doubled during our stay and might have reached a factor of 3 increase in the subsequent time by nitrate supply and excess phosphate.

인산염 대체제로서 치아씨드가 첨가된 건조 소시지의 품질 특성

Phosphate is a widely used food additive for manufacturing processed meat product due to its water holding capacity by increasing the pH of meat batter. However, excessive phosphate consumption has been associated with the occurrence of some diseases including metabolic bone disease and chronic kidney disease. Therefore, this study evaluated the quality properties of chia seed (CS) as a replacement for phosphate in dry sausage. CS [1%–3% (w/w)] was added to produce dry sausage as a phosphate replacer. Quality characteristics including pH, emulsion stability and lipid oxidation were significantly improved in CS-added dry sausage, compared with phosphate free-dry sausage (p&lt;0.05). The lower lightness, redness and yellowness of CS significantly reduced color parameters of dry sausages (p&lt;0.05). Particularly, addition of 3% CS has the highest apparent viscosity and the lowest shear force among the groups (p&lt;0.05). Collectively, our data suggest that CS can replace phosphate in dry sausage without compromising quality characteristics.