Evolutionary History Research Articles

Breathless in the Motor City: Unveiling the Legacy, Challenges, and Mitigation Strategies of Air Pollution in Detroit

This paper delves into the historical trajectory, current status, and consequential ramifications of air pollution in the city of Detroit. With a specific emphasis on the pollutants NO2, SO2, PM2.5, and O3, the study scrutinizes the health implications and societal burdens arising from the escalating air pollution levels against the backdrop of Detroit's historical evolution. Despite regulatory endeavors, Detroit's enduring role as an industrial and manufacturing hub substantiates the escalating health and economic toll of air pollution. These costs disproportionately impact vulnerable demographics, including the elderly, children, and individuals with chronic illnesses. The paper underscores racial disparities in the distribution of this burden. To address these challenges, the paper advocates for a mitigation strategy inspired by the European Union's NEC Directive. The proposed approach involves intensified monitoring of pollutants, implementation of targeted policies, and enhanced industrial regulations. Ultimately, this paper comprehensively examines the historical legacy, contemporary, and future dimensions of air pollution in Detroit, delineating the profound impact on the city and its residents, while offering strategic recommendations for mitigation.

Repeated horizontal acquisition of lagriamide-producing symbionts in Lagriinae beetles.

Microbial symbionts associate with multicellular organisms on a continuum from facultative associations to mutual codependency. In some of the oldest intracellular symbioses there is exclusive vertical symbiont transmission, and co-diversification of symbiotic partners over millions of years. Such symbionts often undergo genome reduction due to low effective population sizes, frequent population bottlenecks, and reduced purifying selection. Here, we describe multiple independent acquisition events of closely related defensive symbionts followed by genome erosion in a group of Lagriinae beetles. Previous work in Lagria villosa revealed the dominant genome-eroded symbiont of the genus Burkholderia produces the antifungal compound lagriamide and protects the beetle's eggs and larvae from antagonistic fungi. Here, we use metagenomics to assemble 11 additional genomes of lagriamide-producing symbionts from seven different host species within Lagriinae from five countries, to unravel the evolutionary history of this symbiotic relationship. In each host species, we detected one dominant genome-eroded Burkholderia symbiont encoding the lagriamide biosynthetic gene cluster (BGC). Surprisingly, however, we did not find evidence for host-symbiont co-diversification, or for a monophyly of the lagriamide-producing symbionts. Instead, our analyses support at least four independent acquisition events of lagriamide-encoding symbionts and subsequent genome erosion in each of these lineages. By contrast, a clade of plant-associated relatives retained large genomes but secondarily lost the lagriamide BGC. In conclusion, our results reveal a dynamic evolutionary history with multiple independent symbiont acquisitions characterized by high degree of specificity. They highlight the importance of the specialized metabolite lagriamide for the establishment and maintenance of this defensive symbiosis.

Deciphering Pteronia's evolution in the Cape Floristic Region: A comprehensive study disputes polyploid deficiency and affirms diploid radiation.

The Greater Cape Floristic Region (GCFR) is renowned for its exceptional biodiversity, accommodating over 11 000 plant species, notable degree of endemism, and substantial diversification within limited plant lineages, a phenomenon ascribed to historical radiation events. While both abiotic and biotic factors contribute to this diversification, comprehensive genomic alterations, recognized as pivotal in the diversification of angiosperms, are perceived as uncommon. This investigation focuses on the genus Pteronia, a prominent representative of the Asteraceae family in the GCFR. Employing NGS-based HybSeq and RADSeq methodologies, flow cytometry, karyology, and ecological modeling, we scrutinize the intricacies of its polyploid evolution. Phylogenetic reconstructions using 951 low-copy nuclear genes confirm Pteronia as a well-supported, distinct clade within the tribe Astereae. The ingroup displays a structure indicative of rapid radiation likely antedating polyploid establishment, with the two main groups demarcated by their presence or absence in the fynbos biome. Genome size analysis encompasses 1293 individuals across 347 populations, elucidating significant variation ranging from 6.1 to 34.2 pg (2C-value). Pteronia demonstrates substantially large genome sizes within Astereae and phanerophytes. Polyploidy is identified in 31% of the studied species, with four discerned ploidy levels (2x, 4x, 6x, 8x). Cytotypes exhibit marked distinctions in environmental traits, influencing their distribution across biomes and augmenting their niche differentiation. These revelations challenge the presumed scarcity of polyploidy in the Cape flora, underscoring the imperative need for detailed population studies. The intricate evolutionary history of Pteronia, characterized by recent polyploidy and genome size variation, contributes substantially to the comprehension of diversification patterns within the GCFR biodiversity hotspot.

Deciphering Pteronia's evolution in the Cape Floristic Region: A comprehensive study disputes polyploid deficiency and affirms diploid radiation.

The Greater Cape Floristic Region (GCFR) is renowned for its exceptional biodiversity, accommodating over 11 000 plant species, notable degree of endemism, and substantial diversification within limited plant lineages, a phenomenon ascribed to historical radiation events. While both abiotic and biotic factors contribute to this diversification, comprehensive genomic alterations, recognized as pivotal in the diversification of angiosperms, are perceived as uncommon. This investigation focuses on the genus Pteronia, a prominent representative of the Asteraceae family in the GCFR. Employing NGS-based HybSeq and RADSeq methodologies, flow cytometry, karyology, and ecological modeling, we scrutinize the intricacies of its polyploid evolution. Phylogenetic reconstructions using 951 low-copy nuclear genes confirm Pteronia as a well-supported, distinct clade within the tribe Astereae. The ingroup displays a structure indicative of rapid radiation likely antedating polyploid establishment, with the two main groups demarcated by their presence or absence in the fynbos biome. Genome size analysis encompasses 1293 individuals across 347 populations, elucidating significant variation ranging from 6.1 to 34.2 pg (2C-value). Pteronia demonstrates substantially large genome sizes within Astereae and phanerophytes. Polyploidy is identified in 31% of the studied species, with four discerned ploidy levels (2x, 4x, 6x, 8x). Cytotypes exhibit marked distinctions in environmental traits, influencing their distribution across biomes and augmenting their niche differentiation. These revelations challenge the presumed scarcity of polyploidy in the Cape flora, underscoring the imperative need for detailed population studies. The intricate evolutionary history of Pteronia, characterized by recent polyploidy and genome size variation, contributes substantially to the comprehension of diversification patterns within the GCFR biodiversity hotspot.

Historical newspapers unlock new insights into the evolution of seafood value chains in Brazil

IntroductionThis study examines the understudied historical evolution of the seafood value chain in southern Brazil by analysing newspaper articles from Santa Catarina state, published between 1855 and 2019.MethodsThrough a meticulous review of 598 selected articles, we reconstruct the development and transformation of small-scale fisheries (SSF) and their interaction with the emerging industrial fishing sector.Results and discussionOur findings highlight the central role of SSF in the seafood value chain, contributing significantly to production, processing, retail, and trade, despite increasing competition and challenges from industrial fishing and aquaculture. The analysis reveals shifts in fish species targeted, processing methods employed, market dynamics, and consumption patterns over time. Notably, the study uncovers the resilience and adaptability of SSF in maintaining their importance to local economies and food security, amidst technological, economic, and regulatory changes. Furthermore, it underscores the often-overlooked contributions of women in seafood value chains, advocating for their equitable recognition. By providing a comprehensive and multi-faceted historical perspective, this research can inform contemporary policy-making, aiming to foster more resilient, inclusive, and sustainable seafood value chains in Brazil.

Evolution-aware Constraint Derivation Approach for Software Remodularization

Existing software clustering techniques tend to ignore prior knowledge from domain experts, leading to results (suggested big-bang remodularization actions) that can not acceptable to developers. Incorporating domain experts knowledge or constraints during clustering ensures the obtained modularization aligns with developers’ perspectives, enhancing software quality. However, manual review by knowledgeable domain experts for constraint generation is time-consuming and labor-intensive. In this paper, we propose an evolution-aware constraint derivation approach, Escort, which automatically derives clustering constraints based on the evolutionary history from the analyzed software. Specifically, Escort can serve as an alternative approach to derive implicit and explicit constraints in situations where domain experts are absent. In the subsequent constrained clustering process, Escort can be considered as a framework to help supplement and enhance various unconstrained clustering techniques to improve their accuracy and reliability. We evaluate Escort based on both quantitative and qualitative analysis. In quantitative validation, Escort, using generated clustering constraints, outperforms seven classic unconstrained clustering techniques. Qualitatively, a survey with developers from five IT companies indicates that 89% agree with Escort’s clustering constraints. We also evaluate the utility of refactoring suggestions from our constrained clustering approach, with 54% acknowledged by project developers, either implemented or planned for future releases.

Evolution-aware Constraint Derivation Approach for Software Remodularization

Existing software clustering techniques tend to ignore prior knowledge from domain experts, leading to results (suggested big-bang remodularization actions) that can not acceptable to developers. Incorporating domain experts knowledge or constraints during clustering ensures the obtained modularization aligns with developers’ perspectives, enhancing software quality. However, manual review by knowledgeable domain experts for constraint generation is time-consuming and labor-intensive. In this paper, we propose an evolution-aware constraint derivation approach, Escort, which automatically derives clustering constraints based on the evolutionary history from the analyzed software. Specifically, Escort can serve as an alternative approach to derive implicit and explicit constraints in situations where domain experts are absent. In the subsequent constrained clustering process, Escort can be considered as a framework to help supplement and enhance various unconstrained clustering techniques to improve their accuracy and reliability. We evaluate Escort based on both quantitative and qualitative analysis. In quantitative validation, Escort, using generated clustering constraints, outperforms seven classic unconstrained clustering techniques. Qualitatively, a survey with developers from five IT companies indicates that 89% agree with Escort’s clustering constraints. We also evaluate the utility of refactoring suggestions from our constrained clustering approach, with 54% acknowledged by project developers, either implemented or planned for future releases.

Phylogeography and paleoclimatic range dynamics explain variable outcomes to contact across a species' range.

Replicability of divergence after contact is a poorly characterized process, particularly in the contexts of phylogeography and postglacial range dynamics within species. Using contact zones located at the leading-, mid- and rear-edges of a species' range, we examined variation in outcomes to contact between divergent lineages of Campanula americana. We investigated whether contact zones vary in quantity and directionality of gene flow, how phylogeographic structure differs between contact zones, and how historic range dynamics may affect outcomes to contact. We found that all contact zones formed at similar times via primary contact yet detected significant admixture in only the rear-edge (RE) contact zone. In the northern leading-edge contact zone and the mid-range Virginia contact zone, gene flow was minimal and asymmetric. In the southern RE contact zone, gene flow was strong and symmetric. Asymmetric admixture in the leading-edge and Virginia contact zones matches the directionality of a known cosmopolitan cytonuclear incompatibility between lineages of C. americana. Our results emphasize the dependence of speciation processes on phylogeographic structure, evolutionary history and range dynamics.

Graph-based mitochondrial genomes of three foundation species in the Saccharum genus.

We reported the graph-based mitochondrial genomes of three foundation species (Saccharum spontaneum, S. robustum and S. officinarum) for the first time. The results revealed pan-structural variation and evolutionary processes in the mitochondrial genomes within Saccharum. Saccharum belongs to the Andropogoneae, and cultivars species in Saccharum contribute nearly 80% of sugar production in the world. To explore the genomic studies in Saccharum, we assembled 15 complete mitochondrial genomes (mitogenome) of three foundation species (Saccharum spontaneum, S. robustum and S. officinarum) using Illumina and Oxford Nanopore Technologies sequencing data. The mitogenomes of the three species were divided into a total of eight types based on contig numbers and linkages. All mitogenomes in the three species encoded 51 unique genes, including 32 protein-coding, 3 ribosomal RNA (rRNA) and 16 transfer RNA (tRNA) genes. The existence of long and short-repeat-mediated recombinations in the mitogenome of S. officinarum and S. robustum was revealed and confirmed through PCR validation. Furthermore, employing comparative genomics and phylogenetic analyses of the organelle genomes, we unveiled the evolutionary relationships and history of the major interspecific lineages in Saccharum genus. Phylogenetic analyses of homologous fragments between S. officinarum and S. robustum showed that S. officinarum and S. robustum are phylogenetically distinct and that they were likely parallel rather than domesticated. The variations between ancient (S. sinense and S. barberi) and modern cultivated species (S. hybrid) possibly resulted from hybridization involving different S. officinarum accessions. Lastly, this project reported the first graph-based mitogenomes of three Saccharum species, and a systematic comparison of the structural organization, evolutionary processes, and pan-structural variation of the Saccharum mitogenomes revealed the differential features of the Saccharum mitogenomes.

Diverse Circular DNA Viral Communities in Blood, Oral, and Fecal Samples of Captive Lemurs

Few studies have addressed viral diversity in lemurs despite their unique evolutionary history on the island of Madagascar and high risk of extinction. Further, while a large number of studies on animal viromes focus on fecal samples, understanding viral diversity across multiple sample types and seasons can reveal complex viral community structures within and across species. Groups of captive lemurs at the Duke Lemur Center (Durham, NC, USA), a conservation and research center, provide an opportunity to build foundational knowledge on lemur-associated viromes. We sampled individuals from seven lemur species, i.e., collared lemur (Eulemur collaris), crowned lemur (Eulemur coronatus), blue-eyed black lemur (Eulemur flavifrons), ring-tailed lemur (Lemur catta), Coquerel’s sifaka (Propithecus coquereli), black-and-white ruffed lemur (Varecia variegata variegata), and red ruffed lemur (Varecia rubra), across two lemur families (Lemuridae, Indriidae). Fecal, blood, and saliva samples were collected from Coquerel’s sifaka and black-and-white ruffed lemur individuals across two sampling seasons to diversify virome biogeography and temporal sampling. Using viral metagenomic workflows, the complete genomes of anelloviruses (n = 4), cressdnaviruses (n = 47), caudoviruses (n = 15), inoviruses (n = 34), and microviruses (n = 537) were determined from lemur blood, feces, and saliva. Many virus genomes, especially bacteriophages, identified in this study were present across multiple lemur species. Overall, the work presented here uses a viral metagenomics approach to investigate viral communities inhabiting the blood, oral cavity, and feces of healthy captive lemurs.

Reconstructing a 300-year history of phosphorus cycle in west Chaohu Lake, China

Anthropogenic activities could significantly increase nutrients loading, especially phosphorus (P), into aquatic systems, leading to eutrophication and disturbance of ecosystems. Detailed investigation of P cycling and its controlling factors in modern lakes could help understand mechanisms behind eutrophication, thus provide suggestions for future environmental management. Here, we investigate evolution history of P and iron (Fe) cycling over the last ∼300 years in west Chaohu Lake, a typical eutrophic lake in East China. The combination of 210Pb-137Cs dating and elemental analysis demonstrates drastic escalation of P input and organic carbon burial since 1960s, coincided with the rapid growth of human population near this region. P phase partitioning data indicate that Fe-bound P (PFe) is the predominant P pool of sediments in Chaohu Lake, which also regulates the evolving trend of reactive P (Preac). Moreover, the highest fraction of PFe is consistent with observations via P K-edge X-ray absorption near edge structure (P XANES). In addition, Fe speciation results show a principal contribution of Fe (hydr)oxides (Feox) and negligible presence of pyrite, suggesting a generally oxygenated depositional environment, where P could be preferentially sequestrated in sediments in association with Fe oxide minerals. Relatively high molar organic carbon/organic P (Corg/Porg) but low Corg/Preac ratios also support limited recycling of Preac in west Chaohu Lake. This study reveals that human activities play an important role in leading to the eutrophication of Chaohu Lake. Future environmental management could utilize the coupling of P and Fe oxides to remove P from water column.

Radula formation and development in Limapontia senestra (Quatrefages, 1844) (Heterobranchia, Gastropoda) from the White Sea

Sacoglossa is a monophyletic group of small suctorial herbivorous Нeterobranchia. Due to the biology of feeding, most sacoglossans (except Cylindrobulla) have a distinctive feeding apparatus, which includes a sucking muscular organ as well as a sacoglossan-type radula with one tooth in a transverse row, which pierces algal cells. Although there is obvious interest in the radular morphology and developmental modes of Sacoglossa, as these characters may provide some insight into the evolutionary history and phylogenetic relationships of Sacoglossa, data on radula formation and morphogenesis in this group are sparse. This paper presents original data on the direct development, formation, and morphogenesis of the radula of Limapontia senestra (Quatrefages, 1844) from the White Sea. Based on the data obtained, we describe the development and formation of the radula. They fit into the general pattern of heterochrony in the radula anlage, characteristic of all gastropods. The small number of odontoblasts in the formation of the adult radula of L. senestra is a distinctive feature of the radula of Heterobranchia.

Plastome evolution of Engelhardia facilitates phylogeny of Juglandaceae

BackgroundEngelhardia (Juglandaceae) is a genus of significant ecological and economic importance, prevalent in the tropics and subtropics of East Asia. Although previous efforts based on multiple molecular markers providing profound insights into species delimitation and phylogeography of Engelhardia, the maternal genome evolution and phylogeny of Engelhardia in Juglandaceae still need to be comprehensively evaluated. In this study, we sequenced plastomes from 14 samples of eight Engelhardia species and the outgroup Rhoiptelea chiliantha, and incorporated published data from 36 Juglandaceae and six outgroup species to test phylogenetic resolution. Moreover, comparative analyses of the plastomes were conducted to investigate the plastomes evolution of Engelhardia and the whole Juglandaceae family.ResultsThe 13 Engelhardia plastomes were highly similar in genome size, gene content, and order. They exhibited a typical quadripartite structure, with lengths from 161,069 bp to 162,336 bp. Three mutation hotspot regions (TrnK-rps16, ndhF-rpl32, and ycf1) could be used as effective molecular markers for further phylogenetic analyses and species identification. Insertion and deletion (InDels) may be an important driving factor for the evolution of plastomes in Juglandoideae and Engelhardioideae. A total of ten codons were identified as the optimal codons in Juglandaceae. The mutation pressure mostly contributed to shaping codon usage. Seventy-eight protein-coding genes in Juglandaceae experienced relaxed purifying selection, only rpl22 and psaI genes showed positive selection (Ka/Ks > 1). Phylogenetic results fully supported Engelhardia as a monophyletic group including two sects and the division of Juglandaceae into three subfamilies. The Engelhardia originated in the Late Cretaceous and diversified in the Late Eocene, and Juglandaceae originated in the Early Cretaceous and differentiated in Middle Cretaceous. The phylogeny and divergence times didn’t support rapid radiation occurred in the evolution history of Engelhardia.ConclusionOur study fully supported the taxonomic treatment of at the section for Engelhardia species and three subfamilies for Juglandaceae and confirmed the power of phylogenetic resolution using plastome sequences. Moreover, our results also laid the foundation for further studying the course, tempo and mode of plastome evolution of Engelhardia and the whole Juglandaceae family.

Kinematic morphology of low-mass galaxies in IllustrisTNG

ABSTRACT The origin of diverse kinematic morphologies observed in low-mass galaxies is unclear. In this study, we investigate the kinematic morphologies of central galaxies with stellar mass $10^{8.5-9.0}{\rm M}_{\odot }$ at $z=0$ in the TNG50-1 cosmological simulation. The majority of the low-mass galaxies in TNG50-1 are dispersion-dominated, consistent with observations. By tracing the evolutionary histories of simulated low-mass galaxies, we find that while most stars form in rotating cold gas discs, the orientation of the star-forming discs relative to the galaxies may evolve with cosmic time. If the cold gas disc remains aligning with the galaxy during its evolution, stars formed at different times share the same rotational direction, leading to a rotation-dominated system. On the contrary, frequent misalignment of cold gas disc would result in a dispersion-dominated system. In addition, we also find that the two-body scattering can have a non-negligible numerical heating effect on the simulated galaxy morphology, especially at central regions of galaxies and for relatively low-mass galaxies. By comparing results of simulations with different resolutions, our results suggest that the simulated morphology of galaxies is roughly reliable when their number of stellar particles exceeds about $10^{4}$, and bulge morphology of galaxies can not be resolved robustly at the resolution level of TNG50-1.

Evolutionary history of an immune protein conserved across all domains of life.

Evolutionary history of an immune protein conserved across all domains of life.

Conservation genomics of the critically endangered Chinese pangolin.

The Chinese pangolin (Manis pentadactyla, MP) has been extensively exploited and is now on the brink of extinction, but its population structure, evolutionary history, and adaptive potential are unclear. Here, we analyzed 94 genomes from three subspecies of the Chinese pangolin and identified three distinct genetic clusters (MPA, MPB, and MPC), with MPB further divided into MPB1 and MPB2 subpopulations. The divergence of these populations was driven by past climate change. For MPB2 and MPC, recent human activities have caused dramatic population decline and small population size as well as increased inbreeding, but not decrease in genomic variation and increase in genetic load probably due to strong gene flow; therefore, it is crucial to strengthen in situ habitat management for these two populations. By contrast, although human activities have a milder impact on MPA, it is at high risk of extinction due to long-term contraction and isolation, and genetic rescue is urgently needed. MPB1 exhibited a relatively healthy population status and can potentially serve as a source population. Overall, our findings provide novel insights into the conservation of the Chinese pangolin and biogeography of the mammals of eastern Asia.

Regeneration recapitulates many embryonic processes, including reuse of developmental regulatory regions.

The wide distribution of regenerative capacity across the animal tree of life raises the question of how regeneration has evolved in distantly-related animals. Given that whole-body regeneration shares the same end-point - formation of a functional body plan - as embryonic development, it has been proposed that regeneration likely recapitulates developmental processes to some extent. Therefore, understanding how developmental processes are reactivated during regeneration is important for uncovering the evolutionary history of regeneration. Comparative transcriptomic studies in some species have revealed shared gene expression between development and regeneration, but it is not known whether these shared expression profiles correspond to shared functions, and which mechanisms activate expression of developmental genes during regeneration. We sought to address these questions using the acoel Hofstenia miamia , which is amenable to studies of both embryonic development and whole-body regeneration. By examining functionally validated regeneration processes during development at single-cell resolution, we found that whereas patterning and cellular differentiation are largely similar, wound response programs have distinct dynamics between development and regeneration. Chromatin accessibility analyses revealed that regardless of playing concordant or divergent roles during regeneration and development, genes expressed in both processes are frequently controlled by the same regulatory regions, potentially via utilization of distinct transcription factor binding sites. This study extends the known correspondence of development and regeneration from broad transcriptomic similarity to include patterning and differentiation processes. Further, our work provides a catalog of regulatory regions and binding sites that potentially regulate developmental genes during regeneration, fueling comparative studies of regeneration.

Anal Fissure and Its Treatments: A Historical Review.

Anal fissure is one of the most common proctological pathologies. It consists of the formation of a longitudinal tear in the anoderm, causing pain and bleeding during and after defecation. When chronic, it can significantly negatively impact the quality of life of the affected patient. Currently, multiple therapeutic options are available, both medical and surgical. The objective of this article is to highlight the historical evolution in the physiopathological understanding and treatment of this disease, underlining the key moments in this history. This is the first article to summarize the milestones in the treatment of anal fissure from ancient to current times.

Influence of rainfall changes from 4.6 to 3.0 ka BP on ceramic pipes

The impact of climate change on human social development has been a topic of research for a long time. Ceramic pipes, which are vital components of urban drainage engineering, are crucial means of managing rain hazards and floods. Exploring the historical evolution of ceramic pipes can help in better understanding the interplay between climate change and human behavior. This study examined the diameters of 86 ceramic pipes unearthed from six cultural sites in central China, including Pingliangtai, Taosi, and Erlitou, dated to 4600–3040 a BP. By combining speleothem records from the excavation sites with precipitation and temperature composite curves for China using Pearson correlation analysis, and verifying with pollen records. The results show that changes in rainfall were the main factor influencing the diameters of the ceramic pipes. This indicates that during this period, ancient people in the Central Plains of China were able to adjust the size of the ceramic pipes to regulate the water management capacity of urban water systems, thereby adapting urban development to climate change.

Long-Read-Based Hybrid Genome Assembly and Annotation of Snow Algal Strain CCCryo 101-99 (cf. Sphaerocystis sp., Chlamydomonadales).

Polar regions harbor a diversity of cold-adapted (cryophilic) algae, which can be categorized into psychrophilic (obligate cryophilic) and cryotrophic (nonobligate cryophilic) snow algae. Both can accumulate significant biomasses on glacier and snow habitats and play major roles in global climate dynamics. Despite their significance, genomic studies on these organisms remain scarce, hindering our understanding of their evolutionary history and adaptive mechanisms in the face of climate change. Here, we present the draft genome assembly and annotation of the psychrophilic snow algal strain CCCryo 101-99 (cf. Sphaerocystis sp.). The draft haploid genome assembly is 122.5 Mb in length and is represented by 664 contigs with an N50 of 0.86 Mb, a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 92.9% (n = 1,519), a maximum contig length of 5.3 Mb, and a guanine-cystosine (GC) content of 53.1%. In total, 28.98% of the genome (35.5 Mb) contains repetitive elements. We identified 417 noncoding RNAs and annotated the chloroplast genome. The predicted proteome comprises 14,805 genes with a BUSCO completeness of 97.8%. Our preliminary analyses reveal a genome with a higher repeat content compared with mesophilic chlorophyte relatives, alongside enrichment in gene families associated with photosynthesis and flagella functions. Our current data will facilitate future comparative studies, improving our understanding of the likely response of polar algae to a warming climate as well as their evolutionary trajectories in permanently cold environments.