Evolutionary History Research Articles

Bounding the Softwired Parsimony Score of a Phylogenetic Network

In comparison to phylogenetic trees, phylogenetic networks are more suitable to represent complex evolutionary histories of species whose past includes reticulation such as hybridisation or lateral gene transfer. However, the reconstruction of phylogenetic networks remains challenging and computationally expensive due to their intricate structural properties. For example, the small parsimony problem that is solvable in polynomial time for phylogenetic trees, becomes NP-hard on phylogenetic networks under softwired and parental parsimony, even for a single binary character and structurally constrained networks. To calculate the parsimony score of a phylogenetic network N, these two parsimony notions consider different exponential-size sets of phylogenetic trees that can be extracted from N and infer the minimum parsimony score over all trees in the set. In this paper, we ask: What is the maximum difference between the parsimony score of any phylogenetic tree that is contained in the set of considered trees and a phylogenetic tree whose parsimony score equates to the parsimony score of N? Given a gap-free sequence alignment of multi-state characters and a rooted binary level-k phylogenetic network, we use the novel concept of an informative blob to show that this difference is bounded by k+1\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$k+1$$\\end{document} times the softwired parsimony score of N. In particular, the difference is independent of the alignment length and the number of character states. We show that an analogous bound can be obtained for the softwired parsimony score of semi-directed networks, while under parental parsimony on the other hand, such a bound does not hold.

Native root hemiparasites form haustorial attachments with multiple invasive and expansive species

Parasitic plants are known for shaping plant communities, mainly by suppressing dominant species. This suppression, in some cases, fosters species coexistence and boosts overall diversity. Recent studies reveal that certain parasitic plants can curb invasive alien plants or expansive native species. In this study, we followed previous case studies, investigating the ability of three common Central European hemiparasitic species to attach to roots and form functional haustoria across a broad range of invasive and expansive hosts. For each host-hemiparasite pair, we posed two questions: (i) Do the hemiparasites produce haustoria on the host's roots or rhizomes? (ii) Does the anatomical structure of the haustoria include all features necessary for their functionality? We cultivated three hemiparasitic species, Melampyrum arvense L., Rhinanthus alectorolophus (Scop.) Pollich, and Odontites vernus subsp. serotinus (Dumort.) Corb., in pots with 18 candidate hosts. After cultivation, we dissected the root systems to determine haustoria abundance and to collect them for anatomical study to assess their functionality. Hemiparasite individuals in each pot were also counted. The hemiparasitic species produced haustoria on the majority of tested hosts (37 out of 44 combinations), with little difference between native expansive and alien invasive plant species. In 13 host-hemiparasite combinations (including eight combinations with invasive species), we identified abundant functional haustoria and good establishment of the hemiparasites. Remarkably, all three hemiparasitic species formed functional haustoria on invasive Asteraceae hosts. By contrast, Melampyrum arvense performed poorly when cultivated with grasses. We identified a series of hemiparasite-host combinations, which should be further tested for the potential hemiparasite effect on host fitness in the field. The abundance and anatomical structure of the haustoria indicates that the recognised low specificity of the hemiparasitic interactions applies also to associations with alien invasive species, with which they do not share a common evolutionary history.

Herbarium specimens as tools for exploring the evolution of fatty acid-derived natural products in plants.

Plants synthesize natural products via lineage-specific offshoots of their core metabolic pathways, including fatty acid synthesis. Recent studies have shed light on new fatty acid-derived natural products and their biosynthetic pathways in disparate plant species. Inspired by this progress, we set out to develop tools for exploring the evolution of fatty-acid derived products. We sampled multiple species from all major clades of euphyllophytes, including ferns, gymnosperms, and angiosperms (monocots and eudicots), and we show that the compositional profiles (though not necessarily the total amounts) of fatty-acid derived surface waxes from preserved plant specimens are consistent with those obtained from freshly collected tissue in a semi-quantitative and sometimes quantitative manner. We then sampled herbarium specimens representing 57 monocot species to assess the phylogenetic distribution and evolution, of two fatty acid-derived natural products found in that clade: beta-diketones and alkylresorcinols. These chemical data, combined with analyses of 26 monocot genomes, revealed a co-occurrence (though not necessarily a causal relationship) between whole genome duplication and the evolution of diketone synthases from an ancestral alkylresorcinol synthase-like polyketide synthase. Limitations of using herbarium specimen wax profiles as proxies for those of fresh tissue seem likely to include effects from loss of epicuticular wax crystals, effects from preservation techniques, and variation in wax chemical profiles due to genotype or environment. Nevertheless, this work reinforces the widespread utility of herbarium specimens for studying leaf surface waxes (and possibly other chemical classes) and reveals some of the evolutionary history of fatty acid-derived natural products within monocots.

Laughing ‘With’ vs. ‘At’: Exploring Emotional Bonds in Media Strategies

This study examines the role of laughter in media strategies. The aim is to distinguish between laughter shared ‘with’ the audience and laughter directed ‘at’ the audience. The research briefly traces the historical evolution of laughter from a repressed and morally criticised expression to a symbol of wealth and empowerment, a transformation facilitated by media diffusion. The subsequent analysis, based on the Portuguese context, seems to indicate a current tendency towards a more empathetic, lighter, and more visibly expressive form of laughter that transcends boundaries such as age, education, and social status, thus fostering a deeper connection with the audience. This study underlines the profound impact of laughter on audience engagement and highlights the central role of communal laughter—laughing ‘with’ the audience—in contemporary media communication. In conclusion, the findings suggest that a more empathetic and less critical approach to audience behaviour through laughter has the potential to attract larger audiences. Indirectly, this emerging trend may be indicative of a growing reluctance among members of contemporary society to tolerate ideas or perspectives contrary to their own.

Subsurface structural trends in Central-Western India: Inferences from magnetotelluric data using the complex MT apparent resistivity tensor

The central-western part of the Indian subcontinent exhibits significant geological diversity and possesses a rich record of different tectonothermal events in the earth's evolutionary history, spanning from the Archean to the present. Precambrian geology in the region is mostly obscured by Cretaceous-Tertiary/Paleocene volcanic eruptions and Proterozoic to Quaternary/recent sedimentary cover. Magnetotelluric (MT) impedance and tipper responses from 146 stations, covering central-western India in a grid fashion with a spacing of ∼55 km, were analyzed to evaluate the subsurface structural trends, dimensionalities, and qualitative characteristics of the electric lithosphere in the region. An advanced Complex Apparent Resistivity Tensor (CART) approach, along with the popular Phase Tensor (PT) approach and induction arrows are utilized to achieve the above goals. The analysis revealed a high degree of 3D induction effects in the data at almost every station and period. The directionality information retrieved from the tensor properties showed that the structural trends in the deep crust and upper mantle of the major geologic domains correlate with the known surface tectonic trend of the respective geologic domain. This study provides vital evidence to support the suspected bifurcation of the Precambrian Aravalli-Delhi Mobile Belt (ADMB) tectonic trend and its extensions into neighboring Kutch, Saurashtra, and Central Indian Tectonic Zone (CITZ) domains. The analysis results indicate a lithospheric scale enhanced conductivity beneath the Malwa plateau, which marks the first major phase of the Reunion mantle plume eruption in India. A critical finding of this study is that the Resistivity Phase Tensor better defines the subsurface resistivity structure and provides much clearer structural information than the conventional Phase Tensor.

Altered assembly paths mitigate interference among paralogous complexes

Protein complexes are fundamental to all cellular processes, so understanding their evolutionary history and assembly processes is important. Gene duplication followed by divergence is considered a primary mechanism for diversifying protein complexes. Nonetheless, to what extent assembly of present-day paralogous complexes has been constrained by their long evolutionary pathways and how cross-complex interference is avoided remain unanswered questions. Subunits of protein complexes are often stabilized upon complex formation, whereas unincorporated subunits are degraded. How such cooperative stability influences protein complex assembly also remains unclear. Here, we demonstrate that subcomplexes determined by cooperative stabilization interactions serve as building blocks for protein complex assembly. We further develop a protein stability-guided method to compare the assembly processes of paralogous complexes in cellulo. Our findings support that oligomeric state and the structural organization of paralogous complexes can be maintained even if their assembly processes are rearranged. Our results indicate that divergent assembly processes by paralogous complexes not only enable the complexes to evolve new functions, but also reinforce their segregation by establishing incompatibility against deleterious hybrid assemblies.

The Phylogenetic Structure Patterns of Angiosperm Species and Their Determinants in East Eurasia

ABSTRACTAimThe Kunming‐Montreal Global Biodiversity Framework requires that evolutionary histories of species should be considered in conservation planning. The phylogenetic structure of species assemblages quantifies species evolutionary histories and increasingly becomes an endeavour for ecologists. Understanding the geographic patterns of phylogenetic structure of species assemblages and their drivers can provide a fundamental reference for conservation planning. Although several theoretical hypotheses based on the effects of contemporary environment, historical climate change and evolutionary niche conservatism had been widely discussed in previous studies, the relative contributions of these hypotheses on phylogenetic structure of angiosperms, especially herbaceous species, remain debated.LocationEast Eurasia.Major Taxa StudiedAngiosperms.MethodsWe compiled distributions of 43,023 angiosperm species in east Eurasia at spatial resolution (100 × 100 km2). Using this newly compiled database and a species‐level phylogeny, we estimated the phylogenetic structure patterns for species with different growth forms. We explored the relationships of these patterns with contemporary environment and historical climate change to test predictions of the tropical niche conservatism hypothesis (TCH) and to compare the contribution of different hypotheses using generalised linear models and hierarchical partitioning.ResultsWe found that phylogenetic structure of angiosperms displayed significant latitudinal gradients. Notably, phylogenetic structure patterns and their drivers differed between woody and herbaceous species. Actual evapotranspiration was the best predictor of phylogenetic structure patterns for all and herbaceous species, while the mean temperature of the coldest quarter was the best predictor for woody species. The effect of historical climate change on phylogenetic structure patterns was weak.Main ConclusionsOur results suggest that the TCH only explains the phylogenetic structure pattern of woody species, not herbaceous species. Moreover, contemporary climate influences the phylogenetic structure of angiosperms in east Eurasian by affecting herbaceous and woody species differently.

The history and future of IPCC special reports: A dual role of politicisation and normalisation

The special reports of the Intergovernmental Panel on Climate Change (IPCC) have a unique character in IPCC scientific assessment. Their main purpose is to address specific timely issues of policy relevance. This article explores the nature and role of IPCC special reports along the lines of three questions: (1) the history (‘where they come from’); (2) the function (‘what they are doing’); and (3) the future (‘where they are going’). In earlier assessment cycles, special reports were characterised mostly as a direct channel for quickly responding to the request from the United Nations Framework Convention on Climate Change (UNFCCC). The conduct of special reports has been gradually institutionalised to embed its planning in the overall scoping of the entire assessment cycle. More recently, they have become a vehicle to consolidate cross-cutting scientific perspectives and serve the diverse needs of the international policy community, not only the UNFCCC. This historical evolution is, in a sense, the result of striving for greater policy relevance. Special reports have a dual political function—namely, turning into the site or object of politicised debates on science (politicisation) and serving as ‘de facto governance’ with the effect of normalising politically contested ideas (normalisation). This dual function is two different faces emerging from the process into which the IPCC is brought to deal with political controversy. The duality of the two functions also manifests inherent tensions that lie behind the IPCC’s ‘policy-relevant but not policy-prescriptive’ principle. For the future of special reports, the IPCC could reconsider the role of special reports in light of the priority over comprehensive assessment reports, the responsiveness to the UNFCCC request and the selection and scope definition of timely topics. However, there will remain a trade-off between provisional science and lasting political impact caused by the future undertaking of IPCC special reports on any topic.

Single domain antibody: Development and application in biotechnology and biopharma.

Heavy-chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and comprised of two heavy chains-only that recognize their cognate antigen by virtue of a single variable domain also referred to as VHH, single domain antibody (sdAb), or nanobody (Nb). These functional HCAbs, serendipitous discovered about three decades ago, are exclusively found in camelids, comprising dromedaries, camels, llamas, and vicugnas. Nanobodies have become an essential tool in biomedical research and medicine, both in diagnostics and therapeutics due to their beneficial properties: small size, high stability, strong antigen-binding affinity, low immunogenicity, low production cost, and straightforward engineering into more potent affinity reagents. The occurrence of HCAbs in camelids remains intriguing. It is believed to be an evolutionary adaptation, equipping camelids with a robust adaptive immune defense suitable to respond to the pressure from a pathogenic invasion necessitating a more profound antigen recognition and neutralization. This evolutionary innovation led to a simplified HCAb structure, possibly supported by genetic mutations and drift, allowing adaptive mutation and diversification in the heavy chain variable gene and constant gene regions. Beyond understanding their origins, the application of nanobodies has significantly advanced over the past 30 years. Alongside expanding laboratory research, there has been a rapid increase in patent application for nanobodies. The introduction of commercial nanobody drugs such as Cablivi, Nanozora, Envafolimab, and Carvykti has boosted confidence among in their potential. This review explores the evolutionary history of HCAbs, their ontogeny, and applications in biotechnology and pharmaceuticals, focusing on approved and ongoing medical research pipelines.

All tangled up: Unraveling phylogenetics and reticulate evolution in the vining ferns, Lygodium (Schizaeales).

Reticulate evolution, often accompanied by polyploidy, is prevalent in plants, and particularly in the ferns. Resolving the resulting non-bifurcating histories remains a major challenge for plant phylogenetics. Here, we present a phylogenomic investigation into the complex evolutionary history of the vining ferns, Lygodium (Lygodiaceae, Schizaeales). Using a targeted enrichment approach with the GoFlag 408 flagellate land plant probe set, we generated large nuclear and plastid sequence datasets for nearly all taxa in the genus and constructed the most comprehensive phylogeny of the family to date using concatenated maximum likelihood and coalescence approaches. We integrated this phylogeny with cytological and spore data to explore karyotype evolution and generate hypotheses about the origins of putative polyploids and hybrids. Our data and analyses support the origins of several putative allopolyploids (e.g., L. cubense, L. heterodoxum) and hybrids (e.g., L. ×fayae) and also highlight the potential prevalence of autopolyploidy in this clade (e.g., L. articulatum, L. flexuosum, and L. longifolium). Our robust phylogenetic framework provides valuable insights into dynamic reticulate evolution in this clade and demonstrates the utility of target-capture data for resolving these complex relationships.

The first record of gars (Ginglymodi: Lepisosteidae) from the Upper Cretaceous of Bulgaria reveals a wider paleogeographic distribution of lepisosteids within the European Archipelago

The bony-fishes of the clade Lepisosteidae, commonly called ‘gars’ or ‘gar-fish’, are a lineage of proficient piscivores with evolutionary history spanning about 150 million years, which are today represented by two genera inhabiting the freshwater and brackish areas of southeastern North America, Central America, and Cuba. This was not the case during the Late Cretaceous when lepisosteids were more diverse and had much wider geographical distribution. Gar fossils, albeit very fragmentary, are a common component of the Upper Cretaceous freshwater and brackish vertebrate assemblages in Europe, yet all of the pre-upper Campanian records come from the western and central parts of the Late Cretaceous European Archipelago. Here we describe new lepisosteid material from Bulgaria, comprising nine teeth and three scales found at the uppermost Santonian–lowermost Campanian Vrabchov Dol vertebrate locality. These fossils represent the easternmost record of gars within the European Archipelago to date. Despite being found in a lagoonal to foreshore deposits, the paleontological content of the locality, the incompleteness and preservational state of the material, as well as the predominantly non-marine ecology of modern and fossils gars suggest that these fossils belong to fishes which inhabited more inland, freshwater environments. The Vrabchov Dol lepisosteids remains are the first record of gars in Bulgaria and one of the rare documented occurrences of Mesozoic osteichthyans in the country. This material expands the paleobiogeographic distribution of the Lepisosteidae within the European Archipelago.

What does not kill Gaia makes her stronger: impacts of external perturbations on biosphere evolution

ABSTRACT Life on Earth has experienced numerous upheavals over its approximately 4 billion year history. In previous work, we have discussed how interruptions to stability lead, on average, to increases in habitability over time, a tendency we called entropic Gaia. Here, we continue this exploration, working with the Tangled Nature Model of co-evolution, to understand how the evolutionary history of life is shaped by periods of acute environmental stress. We find that while these periods of stress pose a risk of complete extinction, they also create opportunities for evolutionary exploration which would otherwise be impossible, leading to more populous and stable states among the survivors than in alternative histories without a stress period. We also study how the duration, repetition and number of refugia into which life escapes during the perturbation affects the final outcome. The model results are discussed in relation to both Earth history and the search for alien life.

The contribution of the Middle Triassic fossil assemblage of Monte San Giorgio to insect evolution

The Triassic represents a critical period for understanding the turnover of insect fauna from the Paleozoic to the Mesozoic following the end-Permian mass extinctions (EPME); however, fossil deposits from the Early-Middle Triassic are scarce. The exceptionally preserved 239 million-year-old fossil insect fauna recorded at Monte San Giorgio (Switzerland), including 248 fossils representing 15 major insect clades is presented here. Besides the exceptional features, including their small size and excellent preservation, the fossils have importance in the evolutionary history of the group. The taxonomic and ecological diversity recovered, including both freshwater (dragonflies and caddisflies) and terrestrial taxa (true bugs and wasps), demonstrates that complex environments sustained a paleocommunity dominated by monurans (thought not to have survived the EPME), midges, and beetles. Interestingly, a blattodean-like fossil bearing an external ootheca was also found, important for understanding Paleozoic roachoids to extant cockroaches’ transition and the evolution of maternal brood care. Moreover, the youngest and first complete specimen of †Permithonidae and the oldest sawfly fossils were discovered. Finally, round-shaped bodies, compatible with seminal capsules or lycophyte spores, were found on the abdomens of several midge-like individuals. If these are spores, non-seed-bearing plants could have been the first entomophilous plants rather than gymnosperms, as recently supposed. Altogether, these fossils contribute substantially to understanding insect evolution and Paleozoic-Mesozoic faunal turnover.

The Historical Evolution of Small and Medium-Sized Enterprises (SMEs) in Greece: The Exploration of Growth Policies Aiming to Accelerate Innovation-Based Economic Transformation and Knowledge Economy

The Historical Evolution of Small and Medium-Sized Enterprises (SMEs) in Greece: The Exploration of Growth Policies Aiming to Accelerate Innovation-Based Economic Transformation and Knowledge Economy

A study on the accumulation model of the Santos basin in Brazil utilizing the source–reservoir dynamic evaluation method

The exploration potential within deep-water petroliferous basins holds great promise for oil and gas resources. However, the dearth of geochemical and isotopic data poses a formidable challenge in comprehending the intricate hydrocarbon charging processes, thereby impeding the comprehensive understanding of hydrocarbon accumulation mechanisms and models. Consequently, the establishment of robust source–reservoir relationships in deep-water petroliferous basins represents a pivotal challenge that significantly influences the exploration strategies and the comprehension of hydrocarbon enrichment dynamics within such basins. In this study, we introduce a novel approach, termed the “source–reservoir dynamic evaluation method,” tailored to investigate reservoir accumulation models in deep-water petroliferous basins. This method uses basin simulation technology to recover the thermal evolution history and hydrocarbon generation and expulsion history of source rocks, and on this basis delimits the hydrocarbon kitchen range. At the same time, the maturity of source rocks corresponding to crude oil and natural gas in typical reservoirs is calculated. Then, when the thermal evolution degree of source rocks adjacent to the reservoir reaches this maturity, the corresponding geological period is the main charging period of hydrocarbon. As a typical deep-water petroliferous basin, the Santos Basin in Brazil has abundant oil and gas reservoirs under the thick salt rock, but there are still some fundamental problems such as unclear oil–gas accumulation process and model. Therefore, in this paper, the main charging periods of typical hydrocarbon reservoirs are determined based on the internal relationship between the thermal evolution history of the main source rocks and the maturity of crude oil and natural gas, and then the hydrocarbon accumulation process is analyzed and the dynamic accumulation model is established. Finally, the favorable prospecting direction is pointed out. The results show that the oil and gas in the Barra Velha Formation in the Santos basin are mainly derived from the Itapema Formation lacustrine shale source rock, and the source rock is mainly developed in the Eastern Sag of the Central Depression, and its main hydrocarbon generation period is from the deposition period of Florianopolis Formation to the deposition period of Santos Formation. The main hydrocarbon expulsion period was from the deposition period of the Santos Formation to the Early deposition of the Iguape Formation. The oil and gas in the Barra Velha Formation were mainly charged from the Late deposition period of the Santos Formation to the Early deposition period of the Iguape Formation. During this period, the hydrocarbon migrated vertically along the normal fault formed in the rift period to the trap of the adjacent inheritance structural highs and accumulated in the reservoir, which was dominated by the accumulation model of the “lower generation-upper reservoir-salt cap”. Since the Barra Velha Formation has the characteristics of near-source accumulation, based on the hydrocarbon expulsion center and hydrocarbon expulsion intensity of the source rock of the Itapema Formation, the distribution ranges of 85% and 50% Pre-salt accumulation probability in the Santos basin were calculated by using the quantitative analysis model of the hydrocarbon distribution threshold. It is suggested that the next oil and gas exploration should be carried out in the paleo-structural highs and slope of Class I favorable area (the hydrocarbon accumulation probability is more than 85%) and Class II favorable area (the hydrocarbon accumulation probability is 85–50%).

Genome-wide SNP assessment of contemporary European red deer genetic structure highlights the distinction of peripheral populations and the main admixture zones in Europe.

Genome-wide technologies open up new possibilities to clarify questions on genetic structure and phylogeographic history of taxa previously studied with microsatellite loci and mitochondrial sequences. Here, we used 736 individual red deer (Cervus elaphus) samples genotyped at 35,701 single nucleotide polymorphism loci (SNPs) to assess the population structure of the species throughout Europe. The results identified 28 populations, with higher degrees of genetic distinction in peripheral compared to mainland populations. Iberian red deer show high genetic differentiation, with lineages in Western and Central Iberia maintaining their distinctiveness, which supports separate refugial ranges within Iberia along with little recent connection between Iberian and the remaining Western European populations. The Norwegian population exhibited the lowest variability and the largest allele frequency differences from mainland European populations, compatible with a history of bottlenecks and drift during post-glacial colonization from southern refugia. Scottish populations showed high genetic distance from the mainland but high levels of diversity. Hybrid zones were found between Eastern and Western European lineages in Central Europe as well as in the Pyrenees, where red deer from France are in close contact with Iberian red deer. Anthropogenic restocking has promoted the Pyrenean contact zone, admixture events in populations on the Isle of Rum and in the Netherlands, and at least partly the admixture of the two main lineages in central-eastern Europe. Our analysis enabled detailed resolution of population structure of a large mammal widely distributed throughout Europe and contributes to resolving the evolutionary history, which can also inform conservation and management policies.

Commemorating the monkey bars, catalyst of debate at the intersection of human evolutionary biology and public health.

Play is an essential part of childhood, and growing attention has focused on the potential health benefits of 'risky' or 'thrill-seeking' play. Such play behavior is readily observed on any playground, where it can sometimes lead to injuries--most often from fall impacts--that require medical attention. Monkey bars account for ~7% of childhood arm fractures in the USA, an alarming statistic that raises difficult questions over its costs and benefits. Many authors view monkey bars as a public health hazard, but it is plausible that our childhood impulse toward thrill-seeking play is a result of selective pressures throughout our primate evolutionary history. Indeed, emerging evidence suggests that the developmental benefits of thrill-seeking play extend into adulthood, outweighing the occasional costs of injury. Disparate and consequential, these dueling perspectives have fueled debate among health professionals and policymakers, but with little attention to the work of biological anthropologists. Here we call attention to the hominin fossil record and play behaviors of non-human primates, providing a novel perspective that bolsters arguments for the adaptive significance of thrill-seeking play. The moment for such a review is timely, for it commemorates the centennial anniversaries of two playground icons: the jungle gym and monkey bars.

From expansion to efficiency: Machine learning-based forecasting of Japan's building material stocks under demographic declines

Japan's unique demographic trajectory, marked by population decline and aging, coupled with continued urbanization, presents distinct challenges for aligning built environment capacity with resource efficiency. This study aims to investigate the historical evolution and project future scenarios of building material stock (MS) and their spatial distribution across Japan's three major metropolitan areas. Through a comprehensive material flow and stock analysis, the historical accumulation of building materials from 2009 to 2020 was quantified, revealing a dominance of concrete and an increasing overall stock. The contributions of various driving factors to changes in construction areas were explored, identifying population dynamics as the predominant influence. Leveraging shared socioeconomic pathway scenarios (SSPs), this research forecasted building floor area and MS until 2050 under five distinct SSPs. The results indicated an overall reduction across all scenarios, yet with a continued concentration in high-density urban cores. The substantial gap between the highest and lowest projected MS scenarios highlighted opportunities for material conservation and emission reductions through sustainable practices. Sustainable urban development in densely populated regions necessitates a balance between infrastructure provision and environmental conservation, while in sparsely populated areas, the focus shifts to the efficient management and utilization of vacant properties and materials to cope with the impacts of significant population declines. By offering insights into the building floor area and MS implications of Japan's demographic changes, this study underscores the necessity of sustainable urban planning and resource management strategies to navigate the challenges posed by demographic shifts, ultimately contributing to sustainable development and environmental conservation goals.

Riverscape genomics of the endangered freshwater mussel Lampsilis rafinesqueana

Abstract Historical periods of rapid climate change and associated direct and indirect impacts such as glaciation and restructuring of drainage systems are important in shaping biodiversity, lineage divergence, and contemporary genetic variation amongst many aquatic taxa. Through an understanding of the impacts of these ancient changes in land‐ and waterscapes on current diversity, practitioners can account for a species' unique evolutionary history when developing conservation strategies. We investigated intraspecific genetic diversity of the critically endangered freshwater mussel, Lampsilis rafinesqueana, endemic to the interior region of the U.S.A. Our study (1) characterises genetic variation within and amongst populations to infer biogeographic history and (2) applies genetic information to potential conservation strategies. We applied restriction‐site associated DNA sequencing and mitochondrial DNA sequencing to investigate genetic diversity within and amongst populations of L. rafinesqueana from across the species' distribution. We then considered this information in the context of historical riverscape processes and applicability to conservation. Variation within rivers was similar, but we found different genetic populations associated with two major ecoregions, the Central Interior Highlands and Osage Plains. The plains population displayed an Ne value approximately 10‐fold lower than the highlands population. Historical demographic modelling suggests the two populations diverged approximately 1.2 million years ago and then both populations underwent a substantial decrease in population size around 19,700 years ago. These two major events are each correlated with known periods of historic rapid climate change. We identified two distinct populations of a federally endangered mussel, L. rafinesqueana. Contemporary genetic diversity of this species was strongly influenced by direct and indirect impacts of rapid historic shifts in climate. The highlands population has undergone a recent expansion, typical of post‐Pleistocene climate change, while also having a much larger Ne value than the plains population. Our results suggest low levels of gene flow amongst the two distinct populations across generations. We recommend that conservation managers for this species take into account relative difference in diversity between populations when developing management strategies. In many species, assisted gene flow amongst genetically unique populations should only be considered in extreme circumstances; however, in L. rafinesqueana, low levels of assisted migration amongst populations may be a valuable tactic to maintain natural levels of genetic variation. Our study shows that biogeographical frameworks can be useful for developing conservation strategies that serve to retain important genomic variation that represents the evolutionary potential of target freshwater species.

Plohophorini glyptodonts (Xenarthra, Cingulata) from the late Neogene of northwestern Argentina. Insight into their diversity, evolutionary history, and paleobiogeography

Plohophorini glyptodonts (Xenarthra, Cingulata) from the late Neogene of northwestern Argentina. Insight into their diversity, evolutionary history, and paleobiogeography