Native root hemiparasites form haustorial attachments with multiple invasive and expansive species

Abstract

Parasitic plants are known for shaping plant communities, mainly by suppressing dominant species. This suppression, in some cases, fosters species coexistence and boosts overall diversity. Recent studies reveal that certain parasitic plants can curb invasive alien plants or expansive native species. In this study, we followed previous case studies, investigating the ability of three common Central European hemiparasitic species to attach to roots and form functional haustoria across a broad range of invasive and expansive hosts. For each host-hemiparasite pair, we posed two questions: (i) Do the hemiparasites produce haustoria on the host's roots or rhizomes? (ii) Does the anatomical structure of the haustoria include all features necessary for their functionality? We cultivated three hemiparasitic species, Melampyrum arvense L., Rhinanthus alectorolophus (Scop.) Pollich, and Odontites vernus subsp. serotinus (Dumort.) Corb., in pots with 18 candidate hosts. After cultivation, we dissected the root systems to determine haustoria abundance and to collect them for anatomical study to assess their functionality. Hemiparasite individuals in each pot were also counted. The hemiparasitic species produced haustoria on the majority of tested hosts (37 out of 44 combinations), with little difference between native expansive and alien invasive plant species. In 13 host-hemiparasite combinations (including eight combinations with invasive species), we identified abundant functional haustoria and good establishment of the hemiparasites. Remarkably, all three hemiparasitic species formed functional haustoria on invasive Asteraceae hosts. By contrast, Melampyrum arvense performed poorly when cultivated with grasses. We identified a series of hemiparasite-host combinations, which should be further tested for the potential hemiparasite effect on host fitness in the field. The abundance and anatomical structure of the haustoria indicates that the recognised low specificity of the hemiparasitic interactions applies also to associations with alien invasive species, with which they do not share a common evolutionary history.