Field Evidence Research Articles

Long term impact of electrical resistance heating on soil bacterial community based on a field test

Thermal remediation is an effective technology for organic contaminant remediation. However, the application of thermal remediation may have negative effects on soil properties and ecological functions, which requires further investigation. Based on a pilot test of electrical resistance heating remediation (ERH), soil samples were collected at different locations after heating for 116 days. Most soil physicochemical properties were less affected by the heating temperature difference. Application of high temperature increased microbial abundance but inhibited alpha diversity of the bacterial community. More significant changes in microbial communities were observed at temperatures above 60 °C. The genera mainly affected by heating temperature included Flavobacteria, Brockia, and S085, while the increase in temperature also inhibited the abundance of nitrochlorobenzene functional genes. At 140 days after the end of the pilot test, the bacterial community affected by thermal remediation could recover effectively, and the recovery of the bacterial community was not affected by temperature difference during the heating period. This study provides valuable field evidence of the long term impact of soil ERH treatment on soil properties and microbial communities, and provides further references for optimization of remediation performance with coupled technologies.

Enhanced formation of nitrogenous organic aerosols and brown carbon after aging in the planetary boundary layer

Particulate organic nitrates (pON) significantly contribute to the mass of organic aerosol and influence the nitrogen oxides cycle in the atmosphere, but their evolution and lifetime remain uncertain. This study performed simultaneous measurements on the anthropogenically affected surface site and the mountain site on top of the polluted planetary boundary layer (PBL). After aging in the PBL, organic nitrate was converted from primary sources (decreased from 8.7% to 4.3%) to secondary sources (increased from 6.3% to 36.1%), spanning from the surface to the mountain. The evaporation of more volatile inorganic nitrate and the production of secondary organic nitrate during aging in the PBL contributed to the enhanced pON fraction over the top of PBL. The contribution of light absorption by brown carbon increased by 57% at the top of PBL compared to the surface, consistent with the higher fraction of nitrogenous organic aerosols over the mountain. The results provide field evidence that the nitrogenous organic aerosols (OA) may be preserved by adding into secondary OA and significantly contribute to the enhanced importance of brown carbon after aging during vertical transport in the PBL.

No Field Evidence of Grass Fuel Structure effects on Postfire Tree Mortality in Juniperus virginiana

Prescribed fires are an important management tool for containing woody plant encroachment in rangeland ecosystems. Grasses are the dominant fuel type in rangelands. Past work has shown that grass canopy architecture, which varies among grass species, can influence flammability. Whether variation in grass fuel structure can influence postfire plant responses has not yet been tested. To bridge this gap, we set up field burning experiments with different fuel treatments and examined postfire mortality of Juniperus virginiana L. in a tallgrass prairie in southwestern Missouri. We sampled 60 trees and measured tree height and diameter at breast height before the fire. Fuels surrounding each tree were manipulated to vary independently in both fuel load and fuel structure. Flame temperatures were measured during the fire, and both stem and canopy injuries were evaluated 1 d after the fire. We surveyed tree mortality 7 mo after the fire. We found no effects of either fuel load or fuel structure on postfire mortality or on canopy injury in J. virginiana. Canopy injury was a critical fire severity measurement determining postfire mortality in J. virginiana, and taller trees are more fire resilient. Despite laboratory-observed fuel structure effects on flammability, this study finds no evidence for the importance of grass fuel load and canopy architecture in influencing postfire tree response. This result might arise from the low crown depth and low canopy water content of J. virginiana, which can promote canopy fire and result in a high mortality rate across fuel treatments. Notwithstanding the negative results, testing laboratory-based findings in field settings is important for further examining laboratory observations and upscaling individual-level processes to ecosystems to help identify the key ecological processes determining population dynamics and community assembly. Our study also suggests that prescribed fire is an effective tool to remove encroaching J. virginiana in tallgrass prairies at an early stage.

Upward and Downward Prosocial Influence Across Levels of a Social Hierarchy: Field and Experimental Evidence About Authorities of U.S. Counties

Upward and Downward Prosocial Influence Across Levels of a Social Hierarchy: Field and Experimental Evidence About Authorities of U.S. Counties

Insights into the variations of polycyclic aromatic hydrocarbons and their nitrated and oxygenated derivatives in urban airborne PM2.5

Polycyclic aromatic hydrocarbons (PAHs) can be transformed into more toxic species in the atmosphere, but field evidence for their transformation remains rare. Here, polycyclic aromatic compounds (PACs), including PAHs and their nitrated and oxygenated derivatives (NPAHs and OPAHs), in fine particulate matter (PM2.5) were measured in the seasons at multiple locations in a megacity (Guangzhou, China). Molecular diagnostic ratios (MDRs), meteorological factors, and atmospheric oxidant measurements were employed to understand the atmospheric behaviors of PM2.5-bound PACs. The results indicated that similarities/differences in the short-term variations of PAC concentrations between the locations depended on their sources and seasons. The temporal trends in MDRs were more distinct than those in concentrations among the locations. Meteorological factors affected PAC concentrations through a similar pathway (demodulating loadings on PM). Their influence on the ratios can be explained by compounds’ atmospheric half-life, vapor pressure, or particle-size distribution. Temperature was the most powerful factor for both the concentrations and MDRs. Relative humidity was minor, but its positive influence on the reaction to OH radicals was appreciable. The dependence on PM2.5 concentrations suggests that PM plays a more significant role in the sorption or formation of N/OPAHs than in their parent compounds. The OH radical-initiated reaction is the major formation mechanism of N/OPAHs throughout the year, but substantial reactions to NO3 radical in autumn were observed due to high ozone levels. Multiple linear regression revealed that parent PAHs, NO2, and/or O3 are significant contributing factors to the secondary formation of N/OPAHs. Partial least squares-discriminant analysis showed the distinguishing characteristics of summer and autumn PM2.5-bound PACs. This study provides field evidence for the gaseous and heterogeneous reactions of PAHs.

Mitigating Traumatic Brain Injury: A Narrative Review of Supplementation and Dietary Protocols.

Traumatic brain injuries (TBIs) constitute a significant public health issue and a major source of disability and death in the United States and worldwide. TBIs are strongly associated with high morbidity and mortality rates, resulting in a host of negative health outcomes and long-term complications and placing a heavy financial burden on healthcare systems. One promising avenue for the prevention and treatment of brain injuries is the design of TBI-specific supplementation and dietary protocols centred around nutraceuticals and biochemical compounds whose mechanisms of action have been shown to interfere with, and potentially alleviate, some of the neurophysiological processes triggered by TBI. For example, evidence suggests that creatine monohydrate and omega-3 fatty acids (DHA and EPA) help decrease inflammation, reduce neural damage and maintain adequate energy supply to the brain following injury. Similarly, melatonin supplementation may improve some of the sleep disturbances often experienced post-TBI. The scope of this narrative review is to summarise the available literature on the neuroprotective effects of selected nutrients in the context of TBI-related outcomes and provide an evidence-based overview of supplementation and dietary protocols that may be considered in individuals affected by-or at high risk for-concussion and more severe head traumas. Prophylactic and/or therapeutic compounds under investigation include creatine monohydrate, omega-3 fatty acids, BCAAs, riboflavin, choline, magnesium, berry anthocyanins, Boswellia serrata, enzogenol, N-Acetylcysteine and melatonin. Results from this analysis are also placed in the context of assessing and addressing important health-related and physiological parameters in the peri-impact period such as premorbid nutrient and metabolic health status, blood glucose regulation and thermoregulation following injury, caffeine consumption and sleep behaviours. As clinical evidence in this research field is rapidly emerging, a comprehensive approach including appropriate nutritional interventions has the potential to mitigate some of the physical, neurological, and emotional damage inflicted by TBIs, promote timely and effective recovery, and inform policymakers in the development of prevention strategies.

Numerical modelling of upheaval buckling of offshore pipelines with unstressed and stressed initial imperfections

Buried offshore pipelines transporting hydrocarbon at high temperatures and pressures might experience upheaval buckling. Although pipelines are designed to remain in place, field evidence shows that the buried pipeline might displace a significantly large distance in the upward direction. In the present study, the pre- and post-buckling behaviour of offshore pipelines buried in sand is investigated considering the degradation of uplift soil resistance with the upward displacement of the pipe. The soil resistance degradation models are implemented in a finite element program, and the analyses are performed for large upward displacements even when a buckled section moves above the seabed. The simulation results show that the capacity of the pipeline to carry the load generated from an increase in pressure and temperature reduces significantly if post-peak degradation of uplift soil resistance is considered. The effects of the internal pressure modelling approach, uplift soil resistance degradation model, burial depth and pipe dimensions (diameter and thickness) on upheaval buckling for varying initial imperfections are investigated. Based on a simplified model for developing initial stresses in a pipeline during installation, it has been shown that initial stresses primarily affect the upheaval buckling at lower ranges of upward displacement of the pipe.

The role of negative affect in shaping populist support: Converging field evidence from across the globe.

Support for populism has grown substantially during the past 2 decades, a development that has coincided with a marked increase in the experience of negative affect around the world. We use a multimodal, multimethod empirical approach, with data from a diverse set of geographical and political contexts, to investigate the extent to which the rising electoral demand for populism can be explained by negative affect. We demonstrate that negative affect-measured via (a) self-reported emotions in surveys as well as (b) automated text analyses of Twitter data-predicts individual-level populist attitudes in two global surveys (Studies 1a and 1b), longitudinal changes in populist party vote shares at general elections in Europe (Study 2), district-level Brexit voting in the 2016 U.K. referendum (Study 3), and county-level vote shares for Donald Trump in the 2016 and 2020 U.S. presidential elections (Studies 4a and 4b). We find that negative emotions-such as fear and anger as well as more often overlooked low-arousal negative emotions like depression and sadness-are predictive of populist beliefs as well as voting and election results at scale. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Supplemental Material for The Role of Negative Affect in Shaping Populist Support: Converging Field Evidence From Across the Globe

Supplemental Material for The Role of Negative Affect in Shaping Populist Support: Converging Field Evidence From Across the Globe

Resistance of Lepidopteran Pests to Bacillus thuringiensis Toxins: Evidence of Field and Laboratory Evolved Resistance and Cross-Resistance, Mode of Resistance Inheritance, Fitness Costs, Mechanisms Involved and Management Options.

Bacillus thuringiensis (Bt) toxins are potential alternatives to synthetic insecticides for the control of lepidopteran pests. However, the evolution of resistance in some insect pest populations is a threat and can reduce the effectiveness of Bt toxins. In this review, we summarize the results of 161 studies from 20 countries reporting field and laboratory-evolved resistance, cross-resistance, and inheritance, mechanisms, and fitness costs of resistance to different Bt toxins. The studies refer mainly to insects from the United States of America (70), followed by China (31), Brazil (19), India (12), Malaysia (9), Spain (3), and Australia (3). The majority of the studies revealed that most of the pest populations showed susceptibility and a lack of cross-resistance to Bt toxins. Factors that delay resistance include recessive inheritance of resistance, the low initial frequency of resistant alleles, increased fitness costs, abundant refuges of non-Bt, and pyramided Bt crops. The results of field and laboratory resistance, cross-resistance, and inheritance, mechanisms, and fitness cost of resistance are advantageous for predicting the threat of future resistance and making effective strategies to sustain the effectiveness of Bt crops.

Implications of active Gavilgarh/Salbardi fault on the evolution of the late Quaternary landscape in Central India

The morphometric indices (MI) have been calculated along the Gavilgarh/Salbardi Fault (G/SF) to investigate active deformation, tectonic uplift, and basin tilt to find out its impact on the late Quaternary landscape. To explore the tectonic intensity, we computed MI such as stream-length gradient index (SL), hypsometric integral (HI), transverse topographic symmetry factor (T), asymmetry factor (Af), basin elongation ratio (Re), mountain front sinuosity (Smf) and valley floor width to valley height ratio (Vf). The necessary field investigations were carried out on the evolution of the Quaternary landscape to ascertain the various morphometric indices. The evaluated values of SL, HI, Re, Smf, and Vf show the high uplift of the northern block exists as opposed to the southern block, and the tilt and asymmetric character of the basins are shown by T and Af values. The presence of sedimentary landscapes like Quaternary alluvium, triangular facets, natural levees, and boulder-pebbly beds, indicates that the area has recently experienced tectonic activity. The assessed values of MI with supporting field evidence proposed that the northern block is uplifted and the basins are tilted because of the effect of G/SF.

Magma mingling in plagiogranites of the Oman ophiolite suggests an origin by fractional crystallisation

Late-stage plagiogranites in the Oman-UAE ophiolite are frequently associated with layered and massive gabbro bodies, intrusive into the mid-crustal section of the ophiolite. This study reports persuasive field evidence from five different localities for magma-mingling between plagiogranites and melts with a mafic and dioritic composition. It is argued from these relationships that mafic and felsic magmas coexisted in the same magma chamber. On this basis it is suggested that the range of melt compositions is related by a process of fractional crystallisation. The plagiogranites are interpreted as the end-product of this fractionation process and the associated gabbros are thought to be related cumulates. The hypothesis is supported with geochemical evidence which shows from the major element chemistry a continuum of compositions between the most mafic and felsic endmembers (47.6–80.6 wt% SiO2). These compositions ‘capture’ the fractionation process in operation. Modelling these variations using the major and trace element chemistry indicates that the fractionating assemblage comprised olivine-clinopyroxene-plagioclase+/−amphibole and that some plagiogranites contained cumulus plagioclase. The mafic rocks associated with late stage plagiogranite formation are derived from a highly depleted mantle source, which implies melting of an already-depleted mantle. This is consistent with previous models for the origin of the Oman ophiolite and implies a supra-subduction setting for this phase of ocean crust evolution in this ophiolite.

Fault system dynamics of the Kashmir, NW Himalaya, India using continuous GPS observations and geomorphic evidences

We collected data from the continuous Global Positioning System (cGPS) sites across the Kashmir Valley, situated at latitude 34◦N, spanning from 2008 to 2021. Inter-site velocities define a region of approximately 15,000 km2 with broadly distributed strain accumulation at −7.22×10−8 nano strain/year (compression component) and the maximum shear strain γmax of 1.9051×10−7 nano strain/year. The estimated site velocity in the ITRF14 ranges between 30.5±1–42.85±3 mm/yr. It was observed that the average deformation rate of the GPS sites in the Kashmir region ranges between 2.86±1–15.47±3 mm/yr relative to the India fixed reference frame, suggesting a predominant N-S directed compressional tectonic regime. The focal mechanism solutions of the earthquakes in and around the Kashmir Valley suggest dominant thrust faulting followed by normal faulting. Analysis of the vertical component of the GPS time series shows that the northwest segment of the valley subsides at the rate of −1.71± 0.70 mm/yr, while the southeast segment uplifts at the rate of 5.4 ± 0.5 mm/yr. In addition to vertical component, we observed differential movement of the sites relative to IISC site on the northwest and southeast segments. The rate of baseline change of the GPS sites indicates 7.30 ± 0.75 mm/yr extension in SE-NW direction and −5.32 ± 0.75 mm/yr NE-SW compression across and along the Kashmir Valley. Geodetic observations reveal a transition that aligns with the Magam lineament/fault previously identified by Ganju and Khar (1984) using gravity and magnetic data. The observation was supported by the field investigations and remote sensing techniques, confirming the existence of Magam Fault. During the field investigations, various geomorphic expressions of fault were observed, including fault ruptures, fault scarps, offset ridges, deflected drainages/rivers, linear alignment of springs, linear drainage lines, triangular facets and offset Recent sedimentary deposits (Karewas) were observed. The field evidence suggests exposure of normal faults at Kondabal, Nasrullapora, Biru and Radbugh. These exposed extensional structures, trends in NE-SW direction and dip in NW direction with varying offset and dip amount. GPS observations supplemented by geomorphic evidences infer the presence of normal fault ̴ 80 Km extending from northeast to southwest.

Resolving the “megacryst paradox”: Feldspar orientation relationships record crystal mobility in granites

K-feldspar megacrysts are common in silicic plutons, but there is a long-running debate around how they form and what their presence tells us about magmatic systems. Field, textural, and geochemical evidence supports growth in a melt-rich environment, but experimental evidence and phase-equilibria modeling indicate that K-feldspar grows late in the crystallization sequence, when the magma is highly crystalline. We provide a new perspective on this problem by examining the arrangement of plagioclase inclusions within megacrysts to test whether they exhibit the systematic low-energy crystallographic relationships expected from attachment by synneusis in melt-rich environments where crystals have space to rotate. We use electron backscatter diffraction to quantify the crystal orientations and find that the megacrysts’ plagioclase inclusions do occupy these preferred orientations and therefore were incorporated in a melt-rich environment. K-feldspar is also present as an interstitial network, but plagioclase crystals hosted within this network have non-systematic orientations. This transition from systematic to non-systematic plagioclase orientations marks the point at which the crystals formed a rigid, interconnected framework that impeded rotation into low-energy orientations. Phase-equilibria modeling indicates that this transition occurred when the magma was ∼55% crystalline. The remaining ∼45% melt crystallized at the eutectic, forming the interstitial phases. Thus, we resolve the “megacryst paradox”; the megacrysts grew freely in melt, and the groundmass K-feldspar formed after crystal lock-up. Megacrysts therefore provide a detailed textural and chemical record of a critical period in the system’s evolution: the transition from a mobile and potentially eruptible magma to an immobile mush.

Neotectonic impact on drainage development in the Eastern Himalayan foreland basin

The present study intends to divulge the complex drainage evolution in connection to neotectonic controls focusing the Jaldhaka and Raidak River interfluve on the eastern Himalayan Foreland Basin (HFB). It involves analysing the surface and sub-surface changes in the fluvial system's evolution with field and geospatial evidence bearing geomorphic markers, sediment analogy and channel morphological properties. The channel orientation, planimetry and the channel longitudinal process anomaly were attributed solely from the geospatial perspective as surface evidence of neotectonic controls. The results unveil an intense neotectonic control on channel development–from structurally guided channel avulsion that shaped the presently active course of the Torsa River to deformation-guided channel morphological changes. Moreover, relatively higher deflection angles, intense channel sinuosity and tight meandering bends forming along the structural elements symptomizes the structure-controlled drainage development in recent times. Furthermore, sediment analogy from deep well logs and shallow subsurface trenches was analysed to obtain subsidence-driven fluvial sequences and geochronology of sedimentary deformation in channel beds and floodplains. The Optically Stimulated Luminescence (with fading correction) technique-based determination of shallow sub-surface sediment's age indicates occurrences of palaeoseismic events between 1.6 ka (±0.1 ka) and 1.35 ka (±0.2 ka) as well as between 1.35 ka (±0.2 ka) and 1.14 ka (±0.1 ka) had resulted in channel bed sagging while stress impacted the formation of syn-sedimentary folding. The palaeoseismic events that led to relative subsidence are evident in upward sediment stacking (channel fill) sequences being observed at multiple trenches. The deep sub-surface staking of channel floor deposition lenses is also evident of basin subsidence that influenced channel oscillations of reoccupation type.

Slab Driven Quaternary Rock‐Uplift and Topographic Evolution in the Northern‐Central Apennines From Linear Inversion of the Drainage System

AbstractInvestigating rock‐uplift variations in time and space provides insights into the processes driving mountain‐belt evolution. The Apennine Mountains of Italy underwent substantial Quaternary rock uplift that shaped the present‐day topography. Here, we present linear river‐profile inversions for 28 catchments draining the eastern flank of the Northern‐Central Apennines to reconstruct rock‐uplift histories. We calibrated these results by estimating an erodibility coefficient (K) from incision rates and catchment‐averaged erosion rates obtained from cosmogenic‐nuclide data, and we tested whether a uniform or variable K produces a rock‐uplift model that satisfactorily fits independent geochronological constraints. We employ a landscape‐evolution model to demonstrate that our inversion results are reliable despite substantial seaward lengthening of the catchments during uplift. Our findings suggest that a rock‐uplift pulse started around 3.0–2.5 Ma, coinciding with the onset of extension in the Apennines, and migrated southward at a rate of ∼90 km/Myr. The highest reconstructed rock‐uplift rates (>1 km/Myr) occur in the region encompassing the highest Apennine massifs. These results are consistent with numerical models and field evidence from other regions exhibiting rapid rock‐uplift pulses and uplift migration related to slab break‐off. Our results support the hypothesis of break‐off of the Adria slab under the central Apennines and its southward propagation during the Quaternary. Moreover, the results suggest a renewed increase in rock‐uplift rates after the Middle Pleistocene along the Adriatic coast, coeval with recent uplift acceleration along the eastern coast of southern Italy in the Apulian foreland.

The Coherent Magnetic Field of the Milky Way

We present a suite of models of the coherent magnetic field of the Galaxy based on new divergence-free parametric functions describing the global structure of the field. The model parameters are fit to the latest full-sky Faraday rotation measures (RMs) of extragalactic sources and polarized synchrotron intensity (PI) maps from the Wilkinson Microwave Anisotropy Probe and Planck. We employ multiple models for the density of thermal and cosmic-ray electrons in the Galaxy, needed to predict the sky maps of RMs and PI for a given Galactic magnetic field (GMF) model. The robustness of the inferred properties of the GMF is gauged by studying many combinations of parametric field models and electron density models. We determine the pitch angle of the local magnetic field (11° ± 1°), explore the evidence for a grand-design spiral coherent magnetic field (inconclusive), determine the strength of the toroidal and poloidal magnetic halo fields below and above the disk (magnitudes the same for both hemispheres within ≈10%), set constraints on the half-height of the cosmic-ray diffusion volume (≥2.9 kpc), investigate the compatibility of RM- and PI-derived magnetic field strengths (compatible under certain assumptions), and check if the toroidal halo field could be created by the shear of the poloidal halo field due to the differential rotation of the Galaxy (possibly). A set of eight models is identified to help quantify the present uncertainties in the coherent GMF spanning different functional forms, data products, and auxiliary input. We present the corresponding sky maps of rates for axion–photon conversion in the Galaxy and deflections of ultrahigh-energy cosmic rays.

Coronary artery disease multivessel not amenable to revascularization: contemporary cohort

Severe diffuse coronary artery disease with anatomy that is not amenable to revascularization represents a poorly studied entity, with a poorly defined prognosis and prevalence, associated with high morbidity and mortality, poor quality of life and high hospitalization rates. Due to the limited evidence in this clinical field and the absence of contemporary studies, we decided to explore this line of research, determining epidemiological, clinical and prognostic aspects. Analytical, retrospective observational cohort study, carried out in a National Medical Center. The prevalence of three-vessel coronary artery disease not susceptible to revascularization was 12.2%, the majority were men (66%), over 65 years of age, with a high burden of comorbidities: pharmacological management consisted of beta blockers (91.5 %), antiplatelet aggregation (95.3%) and statins (95.3%): cardiovascular mortality was 9.4%, with myocardial infarction occurring in 10.4%: the predictor variables of mortality were chronic kidney disease, age over 70 years, insufficiency mitral valve. Coronary artery disease of three vessels not susceptible to revascularization continues to be a frequent entity, with a high-risk clinical and anatomical profile, with a better contemporary prognosis despite the multiple gaps in knowledge that limit its understanding and treatment.

Establishing the Main Mechanisms for the Accounting Information Governance: Delphi study with accounting experts

This study aims to identify the main mechanisms of Accounting Information Governance. The Delphi method was applied in three stages with accounting experts to evaluate a set of mechanisms for managing accounting information. As a result, a ranking of priority mechanisms is presented, which indicates a list of necessary conditions for better data and information management. Such evidence could be useful for both the practice of accounting and for the training of future professionals who need to be able to face the challenges related to the impact of new technologies and the increasing volume of data and information. In terms of Information Governance, this study adds field evidence to the remaining gaps on this subject regarding the identification of the best mechanisms for using data and information and creating value.

Would reducing chlorophyll content result in a higher photosynthesis nitrogen use efficiency in crops?

AbstractDecreasing antenna size is considered a potential option for improving photosynthesis and increasing yield potential. Reducing chlorophyll content has been employed as a strategy to decrease antenna size. One of the commonly mentioned advantages of this approach is its ability to enhance crop nitrogen use efficiency (NUE); however, there is limited field evidence supporting this claim. In this study, we utilized a rice mutant called p35s‐Ami‐YGL1, which exhibits lower chlorophyll content and smaller antenna size, to investigate the effects of modifying leaf chlorophyll content on tissue nitrogen content and NUE. Our results demonstrate that the nitrogen contents in various tissues, including seed tissue, increased on a weight basis in p35s‐Ami‐YGL1 mutants while exhibiting a decrease in C:N ratio. Simultaneously, we observed a reduction in tissue carbon content along with an increase in the levels of chlorophyll precursors such as Proto IX. Specifically, we observed an upregulation in the expression of genes associated with photosynthetic light reactions and chlorophyll metabolism, while there was no increase in the expression of genes involved in the CBB cycle and nitrogen metabolism. In addition, p35s‐Ami‐YGL1 experienced increased photodamage. These findings suggest that the alterations in the C:N ratio and nitrogen content in plants may be attributed to Proto IX‐mediated photodamage and chloroplast reverse transduction signaling. Besides, these results suggest that the observed increase in tissue nitrogen content in p35s‐Ami‐YGL1 does not reflect an increase in plant nitrogen absorption or use efficiency, rather it is a result of stunted carbon fixation capacity.