Aim: This study was aimed to evaluate the relationship and the effect of surgical nurses' attitudes to evidence-based nursing (EBN) practices on patient safety culture (PSC). 
 Material and Method: The study was descriptive and correlational. The sample consisted of 364 nurses working in the surgical departments of a university hospital. Data were collected by using individual information form, attitude towards EBN scale and PSC scale.
 Results: The average total score of PSC of surgical nurses was 3.60±0.38 and the average total score of attitudes towards EBN practice was 60.07±6.01. There was a strong positive correlation between EBN practices and PSC (r=0.705, p=0.000). In the regression analysis, it was seen that the attitude towards EBN practices, which was the predictor variable, explained approximately 49.7% of the variance in the predicted variable of PSC (F=358.175; R2=0.497; p=0.000).
 Conclusion: It was determined that the attitudes of surgical nurses towards EBN practices and PSC were positive. It was found that surgical nurses with higher attitudes towards EBN practices had more positive attitudes towards PSC. Although PSC is important for nurses working in all clinics, it is more important for nurses working in surgical clinics. Therefore, it is recommended that working conditions should be improved and in-service trainings should be planned in line with the latest evidence findings.