Evaluation Of Antioxidant Activity Research Articles

Quantification of Total Phenols, Total Flavonoids, Total Anthocyanins and Evaluation of Antioxidant and Antiradical Activities of Detarium Senegalense Extracts from Chad

Quantification of Total Phenols, Total Flavonoids, Total Anthocyanins and Evaluation of Antioxidant and Antiradical Activities of Detarium Senegalense Extracts from Chad

Investigating the Medicinal Potential of Lavatera cashmeriana Leaf Extract: Phytochemical Profiling and In Vitro Evaluation of Antimicrobial, Antioxidant, and Anticancer Activities.

This study investigated the medicinal potential of Lavatera cashmeriana, a plant traditionally known for its therapeutic properties. The aim was to identify the phytocompounds in L. cashmeriana leaf extract and evaluate its antibacterial, antioxidant, and anticancer effects. Gas chromatography-mass spectrometry analysis was employed to characterize the phytochemical composition of the ethanol extract derived from L. cashmeriana leaves. The antimicrobial potential was assessed through the well diffusion technique, targeting Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. The 2,2-diphenyl-1-picrylhydrazyl assay was conducted to assess antioxidant capabilities, while cytotoxicity against the A549 cancer cell line was determined via the MTT assay. GC-MS analysis identified ten different compounds, with phytol, 1-Eicosanol, and 2,6,10-trimethyl,14-ethylene-14-pentadecne being the most prevalent. The extract exhibited notable antimicrobial efficacy against all bacteria with MIC values ranging from 62.5 to 250 µg/mL. However, C. albicans did not respond. The extract exhibited antioxidative properties with an IC50 value of 86 µg/mL and cytotoxicity with an IC50 value of 69.95 µg/mL against the A549 cancer cell line. The results derived from this study supported the historical use of L. cashmeriana as a medicinal plant and suggested that it can potentially treat a wide range of medical ailments. The identified phytocompounds and the demonstrated antibacterial, antioxidant, and anticancer effects provide scientific evidence for its medicinal properties. However, further investigations are needed to fully understand its safety profile, efficacy, and mechanism of action before recommending it for therapeutic purposes.

In silico prediction of ADMET parameters and in vitro evaluation of antioxidant and cytotoxic activities promoted by indole-thiosemicarbazone compounds.

Cancer is a complex and multifactorial disease characterized by uncontrolled cell growth and is one of the main causes of death in the world. This work aimed to evaluate a small series of 10 different indole-thiosemicarbazone compounds as potential antitumor agents. This is a pioneering study. For this, the antioxidant and cytotoxic capacity against normal and tumor cells was evaluated. The results showed that the compounds were able to promote moderate to low antioxidant activity for the ABTS radical scavenging assay. ADMET in silico assays showed that the compounds exhibited good oral bioavailability. As for toxicity, they were able to promote low cytotoxicity against normal cells, in addition to not being hemolytic. The compounds showed promising in vitro antitumor activity against the T47D, MCF-7, Jurkat and DU-145 strains, not being able to inhibit the growth of the Hepg2 strain. Through this in vitro study, it can be concluded that the compounds are potential candidates for antitumor agents.

In Vitro Evaluation of Wound Healing, Stemness Potentiation, Antioxidant Activity, and Phytochemical Profile of Cucurbita moschata Duchesne Fruit Pulp Ethanolic Extract.

Wound healing comprises an intricate process to repair damaged tissue. Research on plant extracts with properties to expedite wound healing has been of interest, particularly their ability to enhance the stemness of keratinocyte stem cells. Hence, the present study aims to determine the wound healing and stemness potentiation properties of an ethanolic extract derived from Cucurbita moschata fruit pulp (PKE). Human keratinocytes (HaCaT) and primary skin fibroblast cells were used in this study. The migration of the cells was examined by using a scratch wound healing assay, and spheroid behavior was determined by using a spheroid formation assay. The proteins related to migration and stemness were further measured by using Western blotting to explore the mechanism of action of PKE. The methods used to evaluate PKE's antioxidant properties were 2,2-diphenyl-2-picrylhydrazyl (DPPH) scavenging, ABTS radical scavenging activity, and superoxide anion radical scavenging (SOSA) assays. The phytochemistry of the PKE was investigated using phytochemical screening and high-performance liquid chromatography (HPLC) analysis. The results of this study indicate that nontoxic concentrations of PKE increase the rate of migration and spheroid formation. Mechanistically, PKE increased the expression of the migratory-related protein active FAK (phosphorylated FAK), and the subsequence increased the level of p-AKT. The expression of stem cell marker CD133, upstream protein signaling β-catenin, and self-renewal transcription factor Nanog was increased. The PKE also possessed scavenging properties against DPPH, ABTS, and SOSA. The phytochemistry analyses exhibited the presence of alkaloids, glycosides, xanthones, triterpenes, and steroids. Additionally, bioactive compounds such as ɑ-tocopherol, riboflavin, protocatechuic acid, β-carotene, and luteolin were detected. The presence of these chemicals in PKE may contribute to its antioxidant, stem cell potentiation, and wound-healing effects. The findings could be beneficial in the identification of valuable natural resources that possess the capacity to be used in the process of wound healing through the potentiation of stemness via a readily detectable molecular mechanism.

The most commonly used spices in Thai traditional medicine: in vitro evaluation of anti-hyperglycemic, antioxidant, polyphenol content, and nitric oxide production inhibitory activities.

Diabetes mellitus is a persistent hyperglycemic condition. Thai cuisine and medicine incorporate spices: nutmeg, mace, clove buds, cardamom, cinnamon, and coriander. The in vitro impacts of these spices on anti-diabetic, antioxidant, anti-inflammatory, and total phenolic and flavonoid content were assessed. Alpha-amylase and alpha-glucosidase inhibition assays were conducted. Antioxidant potential was measured through DPPH and ABTS assays. Anti-inflammatory activity was determined by inhibiting nitric oxide generation in RAW 264.7 cells. Total phenolic content was quantified using the Folin Ciocalteu method, while total flavonoid content was estimated via the aluminum chloride colorimetric method. Ethanolic and aqueous extracts of a blend of spices (Siam cardamom, nutmeg, mace, and clove buds), denoted as 4-GlurE and 4-GlurA, displayed concentration-dependent inhibition of alpha-glucosidase, with IC50 values of 0.373 and 0.435 mg/mL, respectively. 4-GlurE and 4-GlurA exhibited antioxidant activity, by ABTS·+ radical and DPPH scavenging capabilities. 4-GlurE demonstrated anti-inflammatory potential by reducing nitric oxide generation (IC50: 43.95 ± 2.47 μg/mL). 4-GlurE and 4-GlurA possessed total phenolic content (TPC) of 122.47 ± 1.12 and 148.72 ± 0.14 mg GAE/g, respectively. 4-GlurE exhibited a higher total flavonoid content (TFC) compared to the aqueous extract (340.33 ± 4.77 and 94.17 ± 3.36 mg QE/g). Cinnamon and clove aqueous extracts were more potent than acarbose in alpha-glucosidase inhibition with the highest antioxidant activity. Polyphenol levels (TPC and TFC) exhibited strong correlations with antioxidant capacity. Findings are consistent with the traditional use of 4-Glur, with cinnamon, for diabetes prevention and treatment.

Garcinia dulcis and Garcinia forbesii King fruit peel extract: Secondary metabolite composition, antioxidant, and elastase inhibitory activity evaluation.

Garcinia dulcis and Garcinia forbesii King are native plants from Indonesia and have tremendous potential as a source of raw medicines based on local wisdom. However, scientific data for strengthening pharmaceuticals are still limited. Therefore, it is necessary to conduct a study to strengthen and develop the potential of both plants using the approach of traditional medicine. This study aimed to explore the secondary metabolite composition and biological activity (antioxidant and antielastase) of both plants. Both samples were extracted using 70% ethanol and microwave-assisted extraction with a microwave power of 120 watts for 15 min. The extract obtained was then screened for phytochemicals using specific reagents. The total phenolic content (TPC) was determined using spectrophotometry with a 96-well microplate reader method. The total flavonoid content (TFC) was determined using the colorimetric method, whereas metabolite profiling analysis was conducted using the UPLC-QToF-MS/MS system. Meanwhile, biological activity was tested for antioxidant activity and antielastase as measured by a microplate reader 96-well spectrophotometry method at specific wavelengths. According to the results, G. dulcis and G. forbesii fruit peel extracts showed positive detection of particular secondary metabolites. TPC and TFC values were 13.98 ± 1.90 mg GAE/g and 10.33 ± 1.90 mg QE/g for G. dulcis and 11.98 ± 2.04 mgGAE/g and 1.96 ± 0.36 mgQE/g for G. forbesii. Metabolite profiling detected some compounds from G. dulcis, including ephedrannin B, hinokiflavone, mahuannin J, and candidate mass C9H12O8, and G. forbesii, including 5-Hydroxy-7,8,2'- trimethoxyflavone, lucialdehyde B, candidate mass C21H39NO4, candidate mass C14H10O6, and candidate mass C14H12O6. Meanwhile, the biological activities (antioxidant and antielastase) were 137.721 μg/mL and 108.893 μg/mL for G. dulcis and 481.948 μg/mL and 250.611 μg/mL for G. forbesii, respectively. Both plants showed different profiles of secondary metabolites and biological activities (antioxidant and antielastase) according to their respective characteristics.

In vitro investigation on Pennisetum purpureum leaf extracts grown in Indonesia of phytochemical components, optical characteristics, and antioxidant-antibacterial activities.

Medicinal plants hold significant cultural significance and play a crucial role in the advancement of potentially safe drugs for the therapy of disease worldwide. Pennisetum purpureum or elephant grass has been used for traditional medications in Indonesia without understanding the phytochemicals of those plants. Herein, our report revolves around the qualitative and quantitative examination of phytochemical components, optical properties, antioxidants, and antibacterial assessments of solvent fractions derived from Pennisetum purpureum leaf. The Pennisetum purpureum leaf was successfully soaked with ethanol, n-hexane, and chloroform. The study aimed to assess the total phenolic content (TPC), total flavonoid content (TFC), and total alkaloid content (TAC) within different of extracts. The optical properties of extract were analyzed by absorption light and photoluminescent. Moreover, evaluation of antioxidant activities of extracts through DPPH free radical scavenging and FRAP assays, followed by an evaluation of their effectiveness in antibacterial therapy against different bacterial strains. The qualitative and quantitative phytochemical of Pennisetum purpureum presented as highest in ethanol TPC (85.5 mg GAE/g extracts), TFC (87.9 mg QE/g extracts), and TAC (86.2 mg ATE/g extracts) as compared to other solvents extract. Pennisetum purpureum extract had antioxidant capacity against DPPH radical and FRAP assay. Furthermore, each of the samples displayed antibacterial effectiveness that was dependent on the dosage towards different strains of bacteria. Our findings clearly demonstrated that Pennisetum purpureum leaf extracts grown in Indonesia containing alkaloid, flavonoid, glycoside, saponin, steroids, tannin, and terpenoids that support its capability as antioxidant and antibacterial.

Evaluation of antioxidant activity of watermelon peels (Citrullus lanatusL) and their application in cake making

Watermelon peels (WP) were treated by ethanol 70% (v/v) to extract flavonoids and phenolic acids then were identified by paper chromatographic technique. Synthetic antioxidant (butylated hydroxytoluene, BHT) and natural antioxidant (WPE) exhibited strong and close antioxidative activities (90.60% and 84.76%, respectively). The watermelon peels extract and BHT were added to sunflower oil at levels 100 and 200 ppm to keep its quality during heating at 180 ± 5°C for 28h. Moreover, the synthetic and natural antioxidants were added to cake made up by sunflower oil at the same levels. Cake was stored at refrigerator at 5°C for 28 days and the lipids were extracted every four days. The results reported that the addition of watermelon peels extract as natural antioxidant to sunflower oil and cake delayed the lipid peroxidation during heating oil and storage of cake.

Comparative Evaluation of Antioxidant Activity in Extracts and Fractions from Leaves and Stem of Geophila obvallata (Schumach.) Didr

Background: Oxidative stress, a condition characterized by an imbalance between reactive oxygen species (ROS) and antioxidant defences, is increasingly implicated in various human diseases. Medicinal plants with potent antioxidant properties hold promise for therapeutic interventions against oxidative stress-related pathologies. This study aimed to comparatively evaluate the antioxidant potential of leaf and stem extracts and fractions derived from Geophila obvallata. Methods: Methanolic extracts of G. obvallata leaves and stems were subjected to solvent partitioning to obtain fractions based on polarity. Total phenolic content (TPC) and total flavonoid content (TFC) were determined. The antioxidant capacities of the extracts and fractions were assessed using established assays, including hydroxyl radical scavenging (HRSA) and ABTS+ cation radical scavenging. Results: The highest concentrations of phenolics (86.35 and 79.72 mg/g tissue gallic acid equivalents – GAE) and flavonoids (67.56 and 64.92 mg/g tissue quercetin equivalents – QE) were observed in the leaf and stem methanol extracts, respectively. Remarkably, the antioxidant activity of both leaf and stem methanol fractions surpassed that of the standard controls (ascorbic acid, α-tocopherol, and trolox). The antioxidant capacity exhibited a decreasing trend across the various extracts and fractions, following the order: methanol > 1-butanol > chloroform > benzene > ethyl acetate > n-hexane > aqueous. These findings suggest superior free radical scavenging abilities in the leaf extracts and fractions compared to the controls.Conclusion: This investigation unveils the significant antioxidant potential of G. obvallata leaf extracts, highlighting their potential efficacy in combating diseases associated with oxidative stress. These findings warrant further exploration to elucidate the underlying mechanisms of action and validate their therapeutic potential in vivo models.

Evaluation of antioxidant activity of phytogenic feed additives

Evaluation of antioxidant activity of phytogenic feed additives

Antioxidant and antibrowning properties of Maillard reaction products in food and biological systems.

Oxidative damage refers to the harm caused to biological systems by reactive oxygen species such as free radicals. This damage can contribute to a range of diseases and aging processes in organisms. Moreover, oxidative deterioration of lipids is a serious problem because it reduces the shelf life of food products, degrades their nutritional value, and produces reaction products that could be toxic. Antioxidants are effective compounds for preventing lipid oxidation, and synthetic antioxidants are frequently added to foods due to their high effectiveness and low cost. However, the safety of these antioxidants is a subject that is being discussed in the public more and more. Synthetic antioxidants have been found to have potential negative effects on health due to their ability to accumulate in tissues and disrupt natural antioxidant systems. During thermal processing and storage, foods containing reducing sugars and amino compounds frequently produce Maillard reaction products (MRPs). Through the chelation of metal ions, scavenging of reactive oxygen species, destruction of hydrogen peroxide, and suppression of radical chain reaction, MRPs exhibit excellent antioxidant properties in a variety of food products and biological systems. Also, the capacity of MRPs to chelate metals makes them as a potential inhibitor of the enzymatic browning in fruits and vegetables. In this book chapter, the methods used for the evaluation of antioxidant activity of MRPs are provided. Moreover, the antioxidant and antibrowning activities of MRPs in food and biological systems is discussed. MRPs can generally be isolated and used as commercial preparations of natural antioxidants.

Ethnopharmacological Knowledge and Antioxidant Propensities of Argania spinosa L. from Morocco.

This work aims to merge ethnopharmacological knowledge with biochemical analysis to enrich our understanding of the significance of the argan tree (Argania spinosa (L.) Skeels) and to valorize its crucial role in the province of Essaouira (Morocco). First, a survey was conducted using semistructured interviews with 325 informants from Essaouira province between February and April 2023. The interviews covered sociodemographic data and information on argan tree uses, whether for therapeutic, cosmetic, or food purposes (i.e., applications, parts used, preparation, and administration). Second, phenolic extracts were prepared from various parts of the argan tree (i.e., leaves, kernels, nut shells, press cake, and oil) and then assessed for their antioxidant potential to scientifically validate their traditional uses. The evaluation of antioxidant activity focused on their free radical scavenging and reducing capacities, using DPPH and FRAP assays. Findings confirmed the cultural significance of the argan tree for the local population, as well as their strong dependence on its products. Indeed, it was noted that argan-based products are widely favored in traditional cuisine, with a prevalence of 83.4%; Amlou is the most commonly consumed food. Therapeutic and cosmetic applications accounted for 48.6% and 28.0%, respectively, predominantly for treating skin and subcutaneous issues (69.5%) and diabetes (19.7%). Argan oil was the most cited argan product used, often consumed raw (97.5%), followed by almonds (22.8%). Cataplasm (26.1%) and maceration (24.6%) were preferred for argan derivative preparation. External application (50.1%) was the primary administration method, followed by oral consumption (38.1%) and massage (27.7%). For in vitro assays, the argan tree could prove to be a promising source of phenolic compounds, especially in the leaves (>4 times richer than other parts, 231.046 ± 5.090 mg GAE/g DW). DPPH and FRAP tests demonstrated notable antiradical potential and reducing power, concentration-dependent. Leaf-derived phenolic extracts exhibited the highest free radical scavenging potential (IC50 = 0.589 ± 0.005 mg/ml) and the best reducing capacity (IC50 = 0.420 ± 0.005 mg/ml), although these potencies remained below the standard used. This study represents valuable documentation that can serve to preserve information on the use of argan products while exploring their phytochemical and pharmacological properties.

Indian gooseberry (Phyllanthus emblica L.) based liposomes: Formulation, characterization, in vitro and ex vivo antioxidant activity evaluation

Indian gooseberry (Phyllanthus emblica L.) based liposomes: Formulation, characterization, in vitro and ex vivo antioxidant activity evaluation

The antioxidant, anti-cholangiocarcinoma, and anti-Opisthorchis viverrini activities of ethanolic extract from Antidesma thwaitesianum fruit

Objectives: The current study was performed to determine the antioxidant, anti-inflammatory, and anti-cholangiocarcinoma (CCA) properties of Antidesma thwaitesianum, also known as “MAO,” whole plant extract on Opisthorchiasis in animal models and CCA cell lines. Materials and Methods: Ethanol was used to extract compounds from the whole ripe fruit. The phytochemical investigation of MAO extract was done to evaluate antioxidant activity, and high-performance liquid chromatography was used to identify the active compounds. The efficacy of MAO extract against OV was evaluated in vivo. The anti- CCA activity was evaluated using superoxide dismutase (SRB), cell cycle arrest, apoptosis, and western blot analyses. Results: MAO extract possessed flavonoid and phenolic contents, antioxidant activity, and an expressed cyanidin-3-O-glycosides content of 0.08 μg/mg extract. MAO extract demonstrated hepatoprotective effects through raised alanine transaminase and alkaline phosphatase levels, as well as an influence on oxidative stress via decreased MDA and increased glutathione, superoxide dismutase, and catalase levels. MAO extract significantly inhibited the migration of CCA cells in a concentration- and time-dependent manner, as well as triggered cell cycle arrest on G1 and activated apoptosis pathways via upregulation of C3, downregulation of cyclin-dependent kinase inhibitor p21, cyclin D, and cyclin-dependent kinases 2 expression. MAO extract inhibited inflammation, which in turn decreased fibrosis in hamsters. It also increased hepatoprotective activity. Conclusion: Our findings demonstrate the potential benefits of MAO extract in both in vitro and animal studies of hamster opisthorchiasis. However, more research should be done to ascertain the mechanisms of activity of MAO extracts and elute bioactive components in order to confirm their safety and examine their clinical applications.

Evaluation of antidiabetic and antioxidant activity of methanolic extract of Limonia acidissima leaves in alloxan-induced diabetic wister albino rats: a randomized controlled experimental study

Objectives: The present study was carried out to find graded-doses antidiabetic and antioxidant activities of methanolic extract of Limonia acidissima (MELA) leaves in rats. Materials and Methods: Male Wistar rats were randomly divided into 6 groups (n=8): Normal control, Diabetic control (Alloxan-induced), and test groups where varying doses of MELA (100mg/kg, 200mg/kg, and 400mg/kg) or Metformin (100mg/kg) administered post-alloxan induction. Random blood sugar (RBS) levels were estimated at specific intervals over a 28-day period. Moreover, antioxidant markers (malondialdehyde, superoxide dismutase, and reduced glutathione) were assessed on day 28. Histopathological examination of pancreatic tissue was conducted. Results: Overall, mean RBS levels decreased at all intervals. There was a significant decrease in median malondialdehyde, an increase in median superoxide dismutase, and reduced glutathione levels (all p-values<0.0001). The change in RBS (p-values<0.0001) and antioxidants (p-value>0.05) levels was greater in Group VI than in Group V. On histopathology, Groups VI and V demonstrated regeneration of necrotic β-cells in the islets, and this was more pronounced than Group IV. Conclusion: Results of our study suggested that MELA possesses dose-dependent significant antidiabetic and antioxidant activity.

Evaluation of Toxicity and Antioxidant Activity of Hydro-Ethanol Leave Extract of Mentha piperita

Mentha piperita has numerous pharmacological, cosmetic and alimental applications due to its ability to produce terpene and terpenoid compounds. Antioxidants are considered as important bioactive compound on account of many health benefits along with their vital role in delaying oxidative rancidity of numerous food. The study was to investigate acute toxicity evaluation and in vitro antioxidant activity of hydro-ethanol leave extracts of M. piperita. The acute toxicity was evaluate in rat according to (OECD). Animals were daily observed for 24 hours after treatment for mortality. The antioxidant activity of the extract was evaluated based on the method such as DPPH radical scavenging activity and reducing power method. The results showed that mortality was not observed in phase one but was observed in phase two. The LD50 by oral route in rat was less than 5000 mg/kg body weight. The percentage of antioxidant activity of leaf extracts of M. piperita was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, reducing power (RP) assay, ferric reducing/antioxidant power assay, and nitric oxide (NO) radical scavenging assay. The analysis revealed that NO scavenging activity is higher than DPPH with extract. NO and DPPH exhibited remarkable antioxidant activity. This indicates that the hydro-ethanol leave extract of M. piperita has a good antioxidant activity.

Evaluation and improvement of antioxidant activity and physicochemical properties of yogurt enriched with Persian gum (Amygdalus scoparia Spach) and fennel (Foeniculum Vulgare) extrac

Evaluation and improvement of antioxidant activity and physicochemical properties of yogurt enriched with Persian gum (Amygdalus scoparia Spach) and fennel (Foeniculum Vulgare) extrac

Evaluation and improvement of antioxidant activity and physicochemical properties of yogurt enriched with Persian gum (Amygdalus scoparia Spach) and fennel (Foeniculum Vulgare) extrac [pdf

Evaluation and improvement of antioxidant activity and physicochemical properties of yogurt enriched with Persian gum (Amygdalus scoparia Spach) and fennel (Foeniculum Vulgare) extrac [pdf

Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antioxidant Activity Evaluation

Gold continues to be one of the oldest dental restorative materials which has been used for dental repairs for more than 4000 years and remains an important metal in the dental sector. In a world where the importance of nanoparticles has been well established and its preparation has become much easier, it is important to analyse if these nanoparticles can be extracted from a plant based source as well. Along with its extraction, assessment of each property of the nanoparticle is essential. A few ingredients used in Ayurveda which can also be found being used in almost every household is pepper and eucalyptus and over the years, its importance has remained constant, if not showing an increase. The aim of this study was to extract gold nanoparticles using Eucalyptus and Piper longum and evaluate the antioxidant activity of the derived gold nanoparticles. Preparation of plant extract was done following which, extraction of gold nanoparticles was performed. Antioxidant properties of the gold nanoparticles were tested by a DPPH assay method and compared against the antioxidant gold standard, butylated hydroxytoluene (BHT). The percentage of absorbance was calculated and data was analyzed. The results demonstrated the presence of elemental gold. Both, plant extract derived AuNPs exhibited significant free-radical scavenging activity indicating that they possess antioxidant effects. Keywords - Eucalyptus, antioxidant, Nanoparticles, Novel technique, Piper longum.

Evaluation of Antioxidant and Dermato-protective activities of Origanum vulgare extracts

Medicinal plants represent an economic and accessible natural reservoir of new molecules with biological activities. This study aims to investigate the potential use of "Origanum vulgare", a medicinal plant native to Skikda-Algeria, as a origin of bioactive compounds with antioxidant and photo-protective properties. To this end, the plant's extracts were tested in vitro using four different methods to measure their ability to inhibit free radicals. The extracts were also tested for their capacity to protect skin from sun's rays by measuring their sun protection factor (SPF). The obtained results showed that Origanum vulgare contained high levels of total polyphenols and flavonols with strong antioxidant and photo-protective properties in its extracts, thus suggesting its potential therapeutic applications as a valuable natural resource for the pharmaceutical industry. This plant was also proven to have a very important inhibition of free radicals where ethyl acetate and N butanol extracts had the best IC50 and A0.5 with significant values in some tests lower than the standards. The SPF measure showed high photo-protective activity with an SPF of 47.29±0.00, 47.30±0.00, and 47.21±0.14 respectively. This suggests that the extracts could potentially be used in cosmetic formulations and as a skincare agent. Overall, the plant of Origanum vulgare can represent an interesting source of bioactive compounds, and consequently, the extracts can be useful in the pharmaceutical field for various therapeutic applications.