ustainable development in agriculture is crucial for ensuring long-term food security, environmental health, and economic viability within the European Union (EU). Given the diverse climatic conditions, economic contexts, and cultural traditions across member states, a comprehensive understanding of the peculiarities and regional differences in sustainable agricultural practices is essential for formulating effective policies and strategies. This article aims to provide a comparative analysis of sustainable development in agricultural enterprises across different EU regions. By examining the unique characteristics, implementation strategies, and outcomes of sustainable practices, the article seeks to highlight best practices, identify regional challenges, and propose recommendations for enhancing sustainability in the EU's agricultural sector. The study employs a multi-faceted approach, including a literature review of existing research, policy analysis of the Common Agricultural Policy (CAP) and national policies, in-depth case studies from representative EU countries, surveys and interviews with key stakeholders, and quantitative and qualitative data analysis. This methodology ensures a thorough examination of the various dimensions of sustainable agriculture across the EU. The comparative analysis reveals significant regional differences in the adoption and effectiveness of sustainable agricultural practices. Western and Northern European countries demonstrate high adoption rates of advanced technologies and sustainable practices, supported by robust policies and financial incentives. Southern Europe focuses on water conservation and traditional practices, facing challenges in modernizing their approaches. Eastern Europe shows gradual progress, constrained by economic and infrastructural limitations but benefiting from EU support. Key findings highlight the importance of tailored regional policies, enhanced financial support, and the need for increased knowledge sharing and capacity building. The article concludes with practical recommendations for policymakers, agricultural enterprises, and other stakeholders to harmonize sustainable practices across the EU. These include strengthening and harmonizing policies, supporting research and innovation, improving access to financial resources, enhancing training and education, and fostering multi-stakeholder collaboration.