Ostrya Carpinifolia Research Articles

Novel Empress SARANI Optimization Algorithm to solve the Electrical Energy -Active Power Loss Reduction and Voltage Stability Enhancement

; This paper proposes Pomarine jaeger Optimization (PJO) algorithm, Tiger hunting Optimization (THO) Algorithm, Desert Reynard and Vixen Inspired Optimization (DRVIO) Algorithm, Lonchodidae optimization (LO) algorithm, Caracal optimization (CO) algorithm, Barasingha optimization (BO) algorithm, Amur leopard optimization (AO) algorithm and Empress SARANI Optimization Algorithm to solve the active power loss reduction problem. Regular actions of Pomarine jaeger have been emulated to model the PJO procedure. In THO algorithm, how the Tiger moves to capture the prey is imitated and formulated. In DRVIO algorithm, Desert Reynard and Vixen burrowing capability and spurt tactic from desolate slayers are imitated to formulate the algorithm. LO algorithm emulates the physiognomies of convergent progression, track reliance, populace development and rivalry in the growth of the Lonchodidae populace in environment. In CO approach, Caracal assaults the designated quarry and then quests the quarry in a dashing procedure. BO algorithm stimulated by the Barasingha existence capability in the slayer subjugated atmosphere. AO algorithm imitates the Amur leopard behaviour. Movement paths, stalking, breeding and death are the some phases in the Amur leopard life cycle. Empress SARANI Optimization Algorithm is designed by integrating Parastylotermes Empress inspired optimization (PEIO) algorithm, Dryocopus martius optimization (DMO) algorithm, Ostrya Carpinifolia Search Optimization (OCSO) Algorithm, Hermitage Activities Inspired optimization (HAIO) algorithm with SARANI algorithm. Validity of Empress SARANI Optimization Algorithm is verified in 24 benchmark functions, IEEE and Practical systems. Real power loss (MW) obtained by projected algorithms for IEEE 57 bus system is PJO-21.99, THO-22.79, DRVIO-21.79, LO-23.16, CO-23.92, BO-22.81, AO- 24.89 and Empress SARANI- 20.91. For IEEE 300 bus system is PJO-395.153, THO-397.398, DRVIO-394.208, LO-398.192, CO-398.397, BO-395.209, AO-399.884 and Empress SARANI-389.217. For IEEE 354 bus system is PJO-336.108, THO-339.563, DRVIO-339.099, LO-340.164, CO-340.592, BO 338.906, AO-342.184 and Empress SARANI-334.098. For practical system - WDN 220 KV is PJO-29. 008, THO-30. 929, DRVIO-28. 519, LO-31.265, CO-31. 893, BO-29.872, AO-32.899, Empress SARANI-26.101

Phytochemical Investigation of an Ostrya carpinifolia L. Extract: an Effective Anti-Pollution Cosmetic Active Ingredient.

Ostrya carpinifolia L., a member of the Betulaceae family, is a tree endemic to the Mediterranean basin that is well known for the hardness of its wood. In this study, we assess the anti-pollution activities of a hydroalcoholic extract of O. carpinifolia twigs using several judiciously selected in vitro cosmetic bioassays. The extract's capacity to counteract excessive production of reactive oxygen species following a cutaneous exposure to atmospheric pollution was evaluated using a combination of several antioxidant assays: DPPH, FRAP and β-carotene bleaching assays. These antioxidant assays were complemented by anti-elastase, anti-collagenase, anti-hyaluronidase and anti-lipoxygenase assays to evaluate the capacity of the extract to preserve the integrity of the skin. The hydroalcoholic extract of O. carpinifolia demonstrates intriguing biological antioxidant activities, with approximately 50% inhibition observed in DPPH and β-carotene assays. Furthermore, its anti-lipoxygenase, anti-hyaluronidase, and anti-collagenase activities are noteworthy, exceeding 50% inhibition. The two major compounds of O. carpinifolia ethanolic extract were isolated and identified as myricitrin (1) and quercitrin (2). Myricitrin and quercitrin exhibit antioxidant and anti-hyaluronidase properties; we explored the correlation of these properties with the activity of the crude hydroalcoholic extract. Notably, these compounds have not been previously described in the Ostrya genus.

Vegetation dynamics and land-use change at the Neolithic lakeshore settlement site of Ploča Mičov Grad, Lake Ohrid, North Macedonia

Detailed knowledge about the interactions between vegetation, climate and land use during the Mesolithic and Neolithic, at the transition from foraging to farming, is still scarce in the Balkans. Here we present a palaeoecological study combining pollen, spores and charcoal found in sedimentary cores from Lake Ohrid, Ploča Mičov Grad, North Macedonia, with a particular focus on the vegetation dynamics during the Late Glacial-Holocene and the Mesolithic-Neolithic transitions. Our record begins at ca. 13,500 cal bp (11,550 cal bc) when partially open vegetation, consisting mainly of Pinus, Abies and deciduous Quercus tree stands grew on the hilly flanks of the bay of Ploča. From 12,650 cal bp (cal 10,700 bc), herbs dominated the record until the onset of the Holocene (ca. 11,700 cal bp; 9750 cal bc), when increasing temperatures led to the establishment of pine-deciduous oak forests including Alnus, Fraxinus ornus, Tilia, Ulmus and Abies. These forests persisted until 7,500 cal bp (cal 5550 bc), when deforestation started due to Neolithic land use. This first phase of Neolithic activities in the Ploča Mičov Grad area precedes the earliest archaeological structures so far recorded by almost 1,000 years. Our data suggest two phases of human land use between 7,500 and 6,300 cal bp (5550–4350 cal bc), when high values of Cerealia type pollen and other cultural indicators indicate intense arable and pastoral farming activities. Once human activities decreased, forests were able to re-establish quickly (within 100–250 years), although the composition changed with disturbance-adapted Ostrya type (mostly Ostrya carpinifolia) and Fagus becoming more important. We conclude that forests were resilient to early human disturbance, despite intensive land use and logging activities gradually leading to forest composition changes. Many of these composition changes can still be seen today, suggesting the legacy of Neolithic farmers is still present in today’s landscape.

Correlation between airborne pollen data and the risk of tick-borne encephalitis in northern Italy

Tick-borne encephalitis (TBE) is caused by a flavivirus that infects animals including humans. In Europe, the TBE virus circulates enzootically in natural foci among ticks and rodent hosts. The abundance of ticks depends on the abundance of rodent hosts, which in turn depends on the availability of food resources, such as tree seeds. Trees can exhibit large inter-annual fluctuations in seed production (masting), which influences the abundance of rodents the following year, and the abundance of nymphal ticks two years later. Thus, the biology of this system predicts a 2-year time lag between masting and the incidence of tick-borne diseases such as TBE. As airborne pollen abundance is related to masting, we investigated whether inter-annual variation in pollen load could be directly correlated with inter-annual variation in the incidence of TBE in human populations with a 2-year time lag. We focused our study on the province of Trento (northern Italy), where 206 TBE cases were notified between 1992 and 2020. We tested the relationship between TBE incidence and pollen load collected from 1989 to 2020 for 7 different tree species common in our study area. Through univariate analysis we found that the pollen quantities recorded two years prior for two tree species, hop-hornbeam (Ostrya carpinifolia) and downy oak (Quercus pubescens), were positively correlated with TBE emergence (R2 = 0.2) while a multivariate model with both tree species better explained the variation in annual TBE incidence (R2 = 0.34). To the best of our knowledge, this is the first attempt at quantifying the correlation between pollen quantities and the incidence of TBE in human populations. As pollen loads are collected by widespread aerobiological networks using standardized procedures, our study could be easily replicated to test their potential as early warning system for TBE and other tick-borne diseases.

Predicting the distribution of European Hop Hornbeam: application of MaxEnt algorithm and climatic suitability models

Predicting the distribution of European Hop Hornbeam: application of MaxEnt algorithm and climatic suitability models

Tree diversity and identity modulate the growth response of thermophilous deciduous forests to climate warming

Tree diversity and species identity are known to play an important role in modulating forest productivity and its capacity to buffer the effects of climatic events. The FunDivEurope exploratory platform allowed us to analyse this modulating effect in a medium‐term time span, after an abrupt rise to a new stable level of the average summer temperature of ca 2°C, in Mediterranean forests in central Italy.This paper aims to answer the following questions: 1) did increasing temperature and drought events affect the growth of thermophilous deciduous forests? 2) Was this effect buffered in mixed stands compared to monocultures? 3) Did co‐occurring tree species with different ecological characteristics, from more mesophilous to more xerophilous, have different responses?In 2012 and 2017, wood cores were collected from 659 trees in 36 plots representative of thermophilous deciduous forests. The selected tree species were Castanea sativa, Ostrya carpinifolia, Quercus cerris, Quercus ilex and Quercus petraea. In the sampling plots, they were present in pure stands and mixtures from two to four species. After measuring annual rings on cores, chronologies of basal area increment were built, and inventory data were used to estimate tree growth. Results showed a strong reduction of growth, lasting at least 18 years, after the temperature rise. Tree diversity significantly reduced the growth drop after the sudden and stable rise in summer average temperature. Tree mixture effect on growth stability appeared to be dependent on the tree species present in the mixture.Temperature rise and associated drought events, even without changes in rainfall, are one of the main challenges that European forests will face in the current scenarios of climate change. Tree diversity can buffer the effects of climate change over periods of at least 15 years and should be considered in forest management plans.

European Hophornbeam Biomass for Energy Application: Influence of Different Production Processes and Heating Devices on Environmental Sustainability

Environmental sustainability has recently shifted towards biodiversity protection via governmental and intergovernmental initiatives (e.g., the UN Millennium Ecosystem Assessment, MA). The life cycle assessment, the widespread method for assessing environmental sustainability, was not created to evaluate impacts on biodiversity. However, several authors recognize its ability to estimate biodiversity loss drivers (impact indices on land use change and ecosystem). The study aims to apply LCA to the forest sector, precisely to the wood–energy chain of Hophornbeam, to cover suggestions of the MA for the biodiversity impact assessment. Six different scenarios for stove (3) and fireplace (3) wood production were analyzed, evaluating two baselines and four alternative scenarios, including sensitivity analyses related to transport distances for the raw materials. The functional unit is 1 MJ of energy. The fireplace combustion scenarios are relatively more sustainable than the stove ones are (2.95–3.21% less). The global warming potential (around 3 g CO2 eq/MJ) is consistent with current European directives on the sustainability of biofuels and scientific literature. The scenarios showed similarities regarding the impact of the categories related to MA drivers. Although biodiversity is protected by limiting forest management, some authors argue that for some species (e.g., Hophornbeam), a rational tree felling could produce biofuels, increasing biodiversity.

Good survival of broadleaf tree species in a four-year-old plantation in the Slovenian Karst

Six broadleaf tree species (Celtis australis L. – Mediterranean hackberry, Quercus petraea (Matt.) Liebl. – sessile oak, Fagus sylvatica L. – European beech, Prunus avium L. – wild cherry, Juglans regia L. – Persian walnut and Acer pseudoplatanus L. – sycamore maple) were planted in 2012 in a trial in the Slovenian Karst on two sites differing in productivity to test their suitability for use in the conversion of old pine stands into ecologically more stable broadleaf forests and to investigate their possible response to the harsher growth conditions predicted in the future. The selected economically interesting tree species have higher timber quality than broadleaves which regenerate naturally (e.g., Ostrya carpinifolia, Fraxinus ornus, Quercus cerris). Measurements were taken in 2017, after four growth seasons. All planted species except Fagus sylvatica had a high survival rate. In total, 70% of all seedlings survived, which shows promising potential. The survival rate was higher at the site on flat terrain than at the site on a slope. Prunus avium was the most successful of all planted species in terms of survival rate, at 83%, and other measured parameters (height, height increment, stem diameter, vitality and quality), and Fagus sylvatica was the least successful, with a survival rate of only 20%. Celtis australis had the highest survival rate, at 87%. Acer pseudoplatanus had the largest differences in measured parameters between the more and less productive sites among all planted species. Quercus petraea showed high resistance to xeric conditions and is expected to be the most successful in conversions. All planted species except Fagus sylvatica show favourable initial potential for the future conversion of Karst pine forests.

A combined approach utilizing UAV 3D imaging methods, in-situ measurements, and laboratory experiments to assess water evaporation and trace element uptake by tree species growing in a red gypsum landfill

The extractive industry is increasingly faced with problems of managing contaminated sites. The red gypsum landfill at the Ochsenfeld site is representative of the typology byproduct storage of the Ti-extraction activity. The management of the elemental content and the water body are the issues at this site. The aim of this study was to evaluate the canopy conductance (gcmax) of various tree species and the content of elements in the leaves, utilizing the opportunity of a demonstration plantation setup in 2014 with sixteen tree species, combined with a growth chamber experiment. We combined the gas exchange measurements with the data from two multispectral cameras with RGB and NIR bands embarked on an unmanned aerial vehicle (UAV). In the field, Ostrya carpinifolia, Maclura pomifera, and Rhus copallina had the highest gcmax of all planted tree species, and the high transpiration rate in O. carpinifolia was confirmed in a pot-based controlled experiment. Except R. copallina, the species with a high Mn content (O. carpinifolia, Betula pendula, and Salix aquatica grandis) had high stomatal conductance. O. carpinifolia could therefore be a species to exploit in the management of landfill leachates, especially in the context of climate change since this species is well adapted to dry environments.

Defoliation, Recovery and Increasing Mortality in Italian Forests: Levels, Patterns and Possible Consequences for Forest Multifunctionality

Forest health and multifunctionality are threatened by global challenges such as climate change. Forest health is currently assessed within the pan-European ICP Forests (International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests) programme through the evaluation of tree crown conditions (defoliation). This paper analyses the results of a 24-year assessment carried out in Italy on 253 permanent plots distributed across the whole forested area. The results evidenced a substantial stability of crown conditions at the national level, according to the usual defoliation thresholds Defoliation > 25% and Defoliation > 60%, albeit with species-specific patterns. Within this apparent temporal stability, an increased fraction of extremely defoliated and dead trees was observed. Extreme defoliation mostly occurred in years with severe summer drought, whereas mortality was higher in the years after the drought. The results for singular species evidenced critical conditions for Castanea sativa Mill. and Pinus species, whereas Quercus species showed a progressive decrease in defoliation. Deciduous species, such as Fagus sylvatica L., Ostrya carpinifolia Scop. and Quercus pubescens Willd. suffer the loss of leaves in dry years as a strategy to limit water loss by transpiration but recover their crown in the following years. The recurrence of extreme heat waves and drought from the beginning of the XXI century may increase the vulnerability of forests, and increased tree mortality can be expected in the future.

Ambrosia beetle response to ethanol concentration and host tree species

AbstractEthanol emitted by stressed trees is an olfactory cue used by ambrosia beetles (Coleoptera; Curculionidae; Scolytinae) to locate susceptible hosts to colonize. In addition, ethanol enhances the growth of ambrosia beetle fungal symbionts, improving colonization. Whether host selection and colonization are affected also by the amount of ethanol produced by stressed trees and by tree species is unclear. To investigate these mechanisms, we induced attacks by ambrosia beetles in bolts of eight tree species by coring and filling them with either 5% or 90% ethanol solutions in water. For each ethanol concentration, bolts of the eight different tree species were replicated six times in a randomized complete block design. Entry holes were used as a proxy for host selection whereas gallery development stage was used as a proxy for colonization. Ethanol concentration differentially affected host selection of the three ambrosia beetles that were active during this study. Anisandrus dispar Fabricius preferentially attacked bolts with 90% ethanol concentration, Xylosandrus crassiusculus (Motschulsky) preferentially attacked bolts with 5% ethanol concentration, and Xyleborinus saxesenii (Ratzeburg) attacked bolts irrespective of ethanol concentration. Colonization of X. crassiusculus reflected the same pattern observed for entry holes. The effect of host tree species on host selection was most prominent for X. saxesenii, while X. crassiusculus established a higher number of developed galleries in Ostrya carpinifolia Scopoli bolts than on five of the other tested tree species. Our results suggest that ethanol concentration and host tree species may influence ecological niche partitioning among ambrosia beetle species.

Classification of forest and shrubland vegetation in Mediterranean Turkey

AbstractQuestionsWhat are the main vegetation types of forest and shrubland vegetation at the alliance level in Mediterranean Turkey? What is their syntaxonomical position? Can we integrate them into the European vegetation classification system? Which environmental factors are the main drivers of the floristic differentiation of vegetation types?LocationSouthern and western Turkey.MethodsWe collected 4,717 vegetation plots of forest and shrubland vegetation in Mediterranean Turkey and performed an unsupervised classification of this data set. We described vegetation types based on the classification results, expert knowledge and information from the literature. We defined diagnostic species and prepared distribution maps for each vegetation type. To support the interpretation of the vegetation types, we determined the most important environmental variables using canonical correspondence analysis.ResultsThe studied vegetation was divided into 21 types related to three vegetation belts: (a) thermo‐ and meso‐mediterranean, comprising coniferous (Pinus brutia, Pinus pinea) and sclerophyllous forests, as well as macchia, garrigue and phrygana; (b) supra‐mediterranean, comprising Pinus nigra subsp. pallasiana forests, thermophilous deciduous forests dominated by various oak species and Ostrya carpinifolia, and forests dominated by temperate species such as Fagus orientalis; and (c) oro‐mediterranean, comprising forests and shrublands dominated by Abies cilicica, Cedrus libani, Juniperus excelsa and Juniperus communis subsp. nana. Elevation was identified as the main environmental driver of the vegetation pattern. Among climatic variables, the most important are the mean temperatures (annual and of driest, coldest, and warmest quarters), minimum temperature of winter, precipitation of warmest and driest quarters and precipitation seasonality. These factors indicate the decreasing effect of the Mediterranean climate with increasing elevation.ConclusionsThe vegetation of Mediterranean Turkey is arranged along climatic gradients depending on elevation and the distance from the Mediterranean Sea. Most vegetation types in this area correspond to the syntaxa accepted in EuroVegChecklist, while others were described as new.

Diversity of Ostrya carpinifolia Forests in Ravine Habitats of Serbia (S-E Europe)

We investigated vegetation in ravine habitats of Serbia, in order to classify hop hornbeam (Ostrya carpinifolia Scop.) forests in syntaxonomic terms, assess the effects of environmental factors on their floristic differentiation, and detect the biodiversity components of the analyzed communities. Both K-means clustering and Bayesian classification revealed five ecologically interpretable groups of forests that belong to the alliances Ostryo carpinifoliae-Fagion sylvaticae, Ostryo carpinifoliae-Tilion platyphylli, Fraxino orni-Ostryion carpinifoliae, Pseudofumario albae-Ostryion carpinifoliae, and Achilleo ageratifoliae-Ostryion carpinifoliae. Canonical correspondence analysis indicated that these alliances are clearly differentiated along a combined light–moisture gradient (from shade and mesic to sunny and xeric variants). The alpha diversity increases from xeric to mesic alliances. A lower alpha diversity in xeric forests may be explained by the stress conditions that prevent mesic species from colonizing the saxatile habitats. Extremely high—almost the greatest possible—values of both the species turnover and beta diversity were detected in all variants of the analyzed forests. Such high diversity may be the result of the strong environmental gradients in ravine habitats. The investigated forests represent an important pool of rare, paleo-endemic species that survived Quaternary glaciations in ravine refugia.

Can we use dendrogeomorphology for the spatial and temporal analysis of less intensive mass movement processes?: A case study of three debris flows in NW and W Slovenia

Debris flows can transport large amounts of material and therefore present a significant threat to infrastructure and human life. In this research, we used tree-ring width analyses to quantify the response of trees to three debris flow events in NW Slovenia (Javoršček, Srpenica) and W Slovenia (Nikova) for which we know the time of origin. We attempted to date these and similar tree responses in the past and compared the patterns between different tree species. Altogether, we sampled 147 trees across a range of tree species (Fagus sylvatica, Pinus sylvestris, Fraxinus excelsior, Fraxinus ornus, Acer pseudoplatanus, Picea abies, Juglans regia, Acer campestre, Tilia cordata and Ostrya carpinifolia), including reference trees that were outside the debris flow fan. For 91 trees, we constructed tree-ring chronologies and used pointer-year analysis to identify years that had abnormal growth. For the remaining trees (mostly Ostrya carpinifolia, Tilia cordata and Acer pseudoplatanus), we either could not accurately distinguish tree rings or two samples from a single tree showed significantly different growth patterns. The growth patterns of Fagus sylvatica and Picea abies following debris flow events showed a weak response at the Javoršček site and no clear responses at the other two sites. Tree species responded similarly at the same locations. Due to the lack of a clear response pattern, we were not able to reconstruct past debris flows.

Recent History, Use and Forgetfulness of the Cypress Forest of Fontegreca (Southern Italy)

The cypress (Cupressus sempervirens) is characterized by a very ancient history linked to the wide employment for the technological properties of its wood and for its symbolic value. Although this tree was often considered as a species introduced in Italy, the first genetic studies showed, instead, the presence of an autochthonous population of C. sempervirens in the forest of Fontegreca (Matese massif, Southern Italy), which constitutes the unique autochthonous cypress woodland present in Southwestern and Western Europe. Therefore, investigations were carried out in selected (using geomorphological criteria) areas of the forest, through soil chemical analysis, identification and 14C dating of soil charcoals. Indeed, we hypothesize that these analyses allow clarifying the history of this woodland characterized by the dominance of the cypress in the forest cover. Areas at medium-low (17–29°) slope gradient on the eastern and southern slopes of the forest were investigated and sampled, following pedological criteria (soil horizons order). Soil morphological and chemical analysis showed humus-rich surface horizons, thin (15–30 cm) and poorly developed (young) soils, overlapping the bedrock limestones. The first soil charcoal analysis data highlighted the presence of a previous landscape characterized by several species (e.g., Pistacia, Ostrya carpinifolia, Juniperus sp. and Pinus sp.) and, probably, by a different forest structure. Ongoing charcoal identification and 14C dating will likely give a better understanding of both (1) the cypress history and (2) the development of this forest landscape.

SEASONAL CHANGE OF CHLOROPHYLL CONTENT (SPAD VALUE) IN SOME TREE AND SHRUB SPECIES

Chlorophyll, which is of vital importance for living things, is the main active ingredient of photosynthesis, and the amount of chlorophyll in the plant can show very sensitive responses to various environmental factors, especially site conditions and plant species. In this study, it was aimed to determine the change of chlorophyll content (SPAD value) at the beginning (20 May) and the end (15 October) of growth period in some native and exotic tree and shrub species in Kanuni campus of Karadeniz Technical University and to reveal the differences between the species. Within the scope of the study, measurements were made on a total of 20 species including five native (Quercus hartwissiana Steven, Fagus orientalis Lipsky, Ulmus minör Mill., Liquidambar orientalis Mill., Quercus pubescens Willd.) and seven exotic (Eucalyptus camadulensis Dehnh., Ginkgo biloba L., Quercus castaneifolia C.A.Mey., Cinnamomum camphora (L.) Sieb., Acer negundo L., Quercus rubra L., Aesculus hippocastanum L.) tree species and seven native (Cercis siliquastrum L., Laurus nobilis L., Osmanthus decorus (Boiss.&Balansa) Kasapligil, Laurocerasus officinalis M.Roem., Ostrya carpinifolia Scop., Corylus avellana L., Arbutus unedo L.) and one exotic (Weigela coraensis Thunb.) shrub species. Chlorophyll content was determined with four replications by portable chlorophyll meter (Minolta SPAD-502, Osaka, Japan) which indirectly measures the amount of chlorophyll in the leaf. The data obtained were subjected to Wilcoxon test, variance analysis (one-way ANOVA) and Duncan’s test by using SPSS 23.0 statistical program. As a result of the study, it was determined that there are statistically significant differences (p

Timeline of Leaf and Cambial Phenology in Relation to Development of Initial Conduits in Xylem and Phloem in Three Coexisting Sub-Mediterranean Deciduous Tree Species

It is unclear how the anticipated climate change will affect the timing of phenology of different tree organs/tissues and thus the whole-tree functioning. We examined the timing of leaf phenology and secondary growth in three coexisting deciduous tree species (Quercus pubescens Willd., Fraxinus ornus L. and Ostrya carpinifolia Scop) from a sub-Mediterranean region in 2019. In addition, we investigated the relationship between leaf and cambial phenology and the onset of the potential functioning of initial conduits, as determined by the completed differentiation process (vessels) or final size (sieve tubes). For this purpose, leaf development was monitored and the microcores of cambium and the youngest phloem and xylem increments were repeatedly collected at 7–10-day intervals during the growing season. The results revealed differences in the timing of leaf development and seasonal radial growth patterns in spring among the studied tree species, depending on wood porosity. We found that cambial cell production started in all cases in the first half of March. However, in ring-porous Q. pubescens and F. ornus, radial growth in the stem occurred more than a month before buds were swollen, whereas in diffuse-porous O. carpinifolia, these two events were detected at almost the same time. The end of cambial cell production occurred earliest in F. ornus (mid-July) and two weeks later also in the other two species. The widest initial earlywood vessels and early phloem sieve tubes were found in Q. pubescens, the narrowest initial earlywood vessels in O. carpinifolia and the narrowest early phloem sieve tubes in F. ornus. This indicates differences in the efficiency of conducting systems among the studied species. This novel approach of studying phloem phenology and anatomy in relation to leaf and xylem development contributes to a better understanding of how different tree species adapt their structure of secondary vascular tissues in response to environmental change.

Improving Air Quality by Nitric Oxide Consumption of Climate-Resilient Trees Suitable for Urban Greening.

Nitrogen oxides (NOx), mainly a mixture of nitric oxide (NO) and nitrogen dioxide (NO2), are formed by the reaction of nitrogen and oxygen compounds in the air as a result of combustion processes and traffic. Both deposit into leaves via stomata, which on the one hand benefits air quality and on the other hand provides an additional source of nitrogen for plants. In this study, we first determined the NO and NO2 specific deposition velocities based on projected leaf area (sV d) using a branch enclosure system. We studied four tree species that are regarded as suitable to be planted under predicted future urban climate conditions: Carpinus betulus, Fraxinus ornus, Fraxinus pennsylvanica and Ostrya carpinifolia. The NO and NO2 sVd were found similar in all tree species. Second, in order to confirm NO metabolization, we fumigated plants with 15NO and quantified the incorporation of 15N in leaf materials of these trees and four additional urban tree species (Celtis australis, Alnus spaethii, Alnus glutinosa, and Tilia henryana) under controlled environmental conditions. Based on these 15N-labeling experiments, A. glutinosa showed the most effective incorporation of 15NO. Third, we tried to elucidate the mechanism of metabolization. Therefore, we generated transgenic poplars overexpressing Arabidopsis thaliana phytoglobin 1 or 2. Phytoglobins are known to metabolize NO to nitrate in the presence of oxygen. The 15N uptake in phytoglobin-overexpressing poplars was significantly increased compared to wild-type trees, demonstrating that the NO uptake is enzymatically controlled besides stomatal dependence. In order to upscale the results and to investigate if a trade-off exists between air pollution removal and survival probability under future climate conditions, we have additionally carried out a modeling exercise of NO and NO2 deposition for the area of central Berlin. If the actually dominant deciduous tree species (Acer platanoides, Tilia cordata, Fagus sylvatica, Quercus robur) would be replaced by the species suggested for future conditions, the total annual NO and NO2 deposition in the modeled urban area would hardly change, indicating that the service of air pollution removal would not be degraded. These results may help selecting urban tree species in future greening programs.

Influences of mature Pinus nigra plantations on the floristic-vegetational composition along an altitudinal gradient in the central Apennines, Italy

Plantations of conifers are widespread in Europe, often occurring outside of their native range. Reduction in species richness linked to increases in altitude has been previously reported for natural forests, although there is a lack of specific studies for mature pine plantations in both the Apennines and Europe. The aim of this study was to quantify the long-term effects of the extensive Pinus nigra plantations carried out between 1900 and 1956 in protected areas on floristic richness, species composition, and ecological traits of the understorey vegetation. We compared 20 mature pine plantations selected along an altitudinal gradient (700-1700 m a.s.l.) with neighbouring deciduous natural forests dominated by Ostrya carpinifolia and Fagus sylvatica, which represent the most widespread forest types in the central Apennines. The results showed that floristic richness follows two distinct altitude trends in pine plantations and natural forests. A strong reduction in species richness with elevation was observed in the natural forests, as well as a consequent increase in the local contribution to beta diversity, with a turnover of the ecological traits of the species. Contrastingly, no significant changes in richness and beta diversity were found for pine plantations in the same altitude range. Indeed, mature pine plantations up to 1400-1500 m a.s.l. showed the presence of many heliophilous and thermophilous species that are usually distributed at lower elevation. We discuss how such pine plantations can mitigate the effect of increasing altitude which is usually observed in the natural forests, slowing down the typical diversification of the forest communities along the altitudinal gradient.

Recognition and Characterization of Forest Plant Communities through Remote-Sensing NDVI Time Series

Phytosociology is a reference method to classify vegetation that relies on field data. Its classification in hierarchical vegetation units, from plant associations to class level, hierarchically reflects the floristic similarity between different sites on different spatial scales. The development of remotely sensed multispectral platforms as satellites enormously contributes to the detection and mapping of vegetation on all scales. However, the integration between phytosociology and remotely sensed data is rather difficult and little practiced despite being a goal for the modern science of vegetation. In this study, we demonstrate how normalized difference vegetation index (NDVI) time series with functional principal component analysis (FPCA) could support the analyses of phytosociologists. The approach supports the recognition and characterization of forest plant communities identified on the ground by the phytosociological approach by using NDVI time series that encode phenological behaviors. The methodology was evaluated in two study areas of central Italy, and it could characterize and discriminate six different forest plant associations that have similar dominant tree species but distinct specific composition: three dominated by black hornbeam (Ostrya carpinifolia) and three dominated by holm oak (Quercus ilex). The methodology was also able to optimize the ground data collection of unexplored areas (from a phytosociological point of view) by using a phenoclustering approach. The obtained results confirmed that by using remote sensing, it is possible to separate and distinguish plant communities in an objective/instrumental way, thus overcoming the subjectivity intrinsic to the phytosociological method. In particular, FPCA functional components (NDVI seasonalities) were significantly correlated with vegetation abundance data variation (Mantel r = 0.76, p < 0.001).