Apis Mellifera Research Articles

Neonicotinoids, Agriculture And Food Security Of Brazilian Honey: A Review

Pesticides used in agriculture have the function of controlling pests, diseases and preventing the development and growth of weeds, increasing the quality and productivity of crops. However, when they reach unwanted targets, such as bees, humans and other living beings, they cause harmful damage to ecosystems. Neonicotinoids, insecticides widely used in monocultures, such as soybeans and corn in Brazil, are especially harmful to bees, whether native or exotic, interfering with the foraging and pollination performance of plants. The global decline in the bee population is related to the use of insecticides, a fact that directly threatens the production of various foods. Honey, the main beekeeping product , is not only a source of food for bees, humans and other animals, but also an indicator of environmental quality, as its composition reflects the conditions of the environment in which it was produced, and determinations made therein can reveal the presence of contaminants such as pesticides and toxic metals providing information about ecosystem health and the presence of environmental contaminants. Furthermore, consuming pesticide-free foods is crucial for human health and environmental sustainability, as the intake of neonicotinoids is associated with several health problems such as hormonal disorders and nervous system diseases and is especially dangerous for children. This article reviews the neonicotinoid insecticides imidacloprid and thiamethoxam and the contamination they cause in the honey of bees Apis mellifera Linnaeus (1758) and Tetragonisca angustula Latreille (1811) in Brazil, evaluating the impacts of these insecticides on bees, the implications for ecosystems and safety to feed

Effect of supplementary diets on Apis mellifera colonies

Effect of supplementary diets on Apis mellifera colonies

Development of a B‐risk

Abstract In specific contract No 12 issued under the framework agreement OC/EFSA/AMU/2019/02, EFSA requested Open Analytics to implement a web application to do a risk assessment for honey bees, solitary bees and bumble bees. The software is developed in R and consists of a WEB‐based tool composed by several modules providing data entry for active substances, uses, metabolites and the modelling of toxicity studies. The application is developed in a modular form such that new modules can be added when available, either by the developers of the application or by EFSA.

Correction to: Higher early than late-season residue load of pesticides in honey bee bread in slovakia

Correction to: Higher early than late-season residue load of pesticides in honey bee bread in slovakia

Evaluating maturity indicators in stingless bee honey: The role of soluble solids over pot condition in harvesting standards

In stingless bee honey (SBH) harvesting, beekeepers often collect honey without accurately assessing its maturation stage, which can impact its physicochemical diversity. This study aimed to evaluate the influence of storage pot condition on SBH maturity by examining honey from open pots (OP) and closed pots (CP). It is commonly assumed that honey in OP is immature and honey in CP is mature. Our physicochemical analyses, including moisture content, soluble solids (°Brix), sugars, and aliphatic organic acids (AOA), tested this hypothesis. The findings revealed that 40 % of SBH samples from OP were mature, challenging the initial assumption. Furthermore, AOA analysis showed that gluconic, acetic, and lactic acids are predominant in SBH, but no direct correlation between AOA concentration and honey maturity was observed. Based on these results, we conclude that OP and CP are not reliable indicators of SBH maturity. This was a preliminary study, providing results that can be further investigated through the validation of the applied methodology and with a larger number of samples. Nevertheless, this study is an important step towards standardizing SBH and assisting producers during the harvest, ultimately optimizing the collection process. To standardize SBH maturity, we recommend using °Brix values, with values above 55 suggesting mature honey with a moisture content below 40 g 100 g⁻¹. Implementing °Brix measurements can improve harvesting practices, enhance honey quality, reduce bee stress, and increase both honey production and market availability.

Harvesting location influences the physicochemical properties, microbiological quality and sensory attributes of honey produced by an Afrotropical stingless bee, Axestotrigona ferruginea

This study investigated the physicochemical properties, sugar profiles, microbiological quality and sensory attributes of the honey produced by an Afrotropical stingless bee, Axestotrigona ferruginea, collected from two locations in Tanzania; Kibiti, representing the coastal mangrove ecosystem, and Siha, representing the northern highland’s Afromontane region. Honey samples from the two locations showed significant variations in moisture content, pH, free acidity, color, 5-hydroxymethylfurfural, total yeast and mold counts, and sensory properties (p < 0.05). However, there was no difference in the sugar profiles of the studied honey samples (p > 0.05). Overall, all honey samples met the East African quality standards for stingless bee honey, except for reducing sugars content. Additionally, all honey samples had acceptable microbial counts, indicating good quality. These findings provide valuable insights into the quality and sensory diversity of stingless bee honey, which can positively influence its acceptability and marketability.

Molecular docking analysis of subtilisin-like alkaline serine protease (SLASP) and laccase with natural biopolymers

Abstract Alkaline serine proteases (ASPs) and laccases (Lacs) have been reported to possess several industrial applications, particularly in the food, cosmetic, and leather industries. Thus, in the present study, eighteen natural biopolymers, including agar, agarose, alginate, kappa carrageenan, cellulose, chitosan pentamer, chitosan oligosaccharide, chondroitin sulfate, dextran, fucoidan, heparin, hyaluronan, lignin, mannan, pectin, phytic acid, pullulan and starch soluble, were studied for their docking behavior, such as subtilisin-like alkaline serine protease (SLASP) KP-43 and Bacillus subtilis laccase (Bs Lac), using the SwissDock method. Additionally, the toxicity toward honey bees and human liver was determined by utilizing the free Bee-Tox and pkCSM web servers, respectively. Bee-Tox analysis demonstrated that four ligands (namely, agar, cellulose, lignin, and pullulan) exhibited acute oral toxicity toward honey bees (Apis mellifera). The docking study revealed that chitosan pentamer and lignin exhibited maximum binding energies of −9.67 and −11.37 kcal/mol against the target proteins SLASP (KP-43) and Lac (Bs Lac), respectively. Interestingly, in the present study, agarose was shown to interact with the His68 and Ser255 amino acid residues of SLASP (KP-43) from KSM-KP43 Bacillus sp. Thus, the current investigation showed the potential of eighteen natural biopolymers as immobilizing agents to prepare ASP and Lac nanoparticles for biomedical applications.

Biological damage of monocrotaline on the brain and intestinal tissues of Apis mellifera

Monocrotaline (MCT) is a toxic alkaloid present in plants, posing a threat to animals in terrestrial ecosystems. However, little is known about its potential impacts on pollinating insects. Here, we report the effects of of MCT on the brains and intestines of foraging honeybees (Apis mellifera). MCT exposure resulted in a reduction in head weight and swelling of the abdomen in honeybees. Additionally, MCT exposure caused morphological damage to the brain, characterized by decreased antioxidant capacity and increased apoptosis, along with intestinal tissue damage that was accompanied by increased antioxidant capacity and apoptosis. Moreover, MCT altered the core gut microbial community structure in honeybees and increased the expression of antimicrobial peptide (AMP) genes in the midgut. These findings indicate that exposure to MCT activates the immune response in the honeybee gut, while the brain does not exhibit an immune response but instead experiences oxidative stress. This study provides a resource for future research exploring interactions between MCT and other insects, and can help deepen our understanding of MCT's potential impacts in ecosystems.

Environmental Biomonitoring of Heavy and Toxic Metals Using Honeybees and Their Products—An Overview of Previous Research

Humans and bees share millennia of history that have resulted in ever-increasing connection and interdependence. Thus, today, it is impossible to ignore the influence of humans on bees, particularly regarding the decrease in their numbers due to environmental contamination. Although they do not cause immediate mortality, heavy and toxic metals, along with dangers such as bee diseases, pesticides, habitat destruction, and climate change, threaten the number of bees and should not be ignored. Honeybees, their colonies, and their products are recognized as accumulators of metals and biological indicators of the presence of these metals in all environmental components. This study is an overview of prominent research from the past three decades on heavy and toxic metal levels in honeybees (Apis mellifera L.), honey, wax, and pollen. This research compares metals such as Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn in natural environments and in an environment where anthropogenic pressure manifests. The presented studies represent a range of research using analytical methods to determine the presence of heavy and toxic metals in different segments of bees and their products, linking these findings with the state of the environment. It has been repeatedly established that if heavy and toxic metals are present in higher concentrations in components of the environment that are under anthropogenic pressure, then their concentrations in bees, honey, and wax will also be higher. By summarizing this research in one place, this study can provide guidelines for future scientific work on this subject, promoting sustainable development through safe beekeeping and healthy bees.

The IMPACT OF HONEYBEE GUT BACTERIA ON THE SURVIVAL OF HONEYBEES EXPOSED TO INSECTICIDES

The widespread use of insecticides poses a significant threat to the health and sustainability of honeybees (Apis mellifera). This study aimed to isolate, identify, and investigate the potential of honeybee gut bacterial strains in mitigating the detrimental effects of insecticides on honeybees and extending their lifespan. The efficacy of seven honeybee gut bacterial strains in reducing insecticide toxicity was evaluated. Through the identification of honeybee gut bacterial isolates using 16s rRNA, seven strains were identified, namely Priestia endophytica, Bacillus subtilis, Bacillus pumilus, Peribacillus frigoritolerans, B. subtilis, Bacillus pumilus, and Bacillus tequilensis. The experimental results revealed that bees treated with these gut bacteria significantly reduced oxidative stress markers and detoxification enzyme levels compared to untreated bees. Moreover, the treated bees demonstrated enhanced immune responses. The bees treated with deltamethrin + intestinal bacteria showed an increase in bees' lifespan to 6.33 days compared to a lifespan of 4 days with deltamethrin only. Similarly, bees treated with acetamiprid intestinal bacteria had further extended to 10.33 days compared to a lifespan of 7.67 days with acetamiprid. These findings suggest that using honeybee gut bacterial strains may serve as a sustainable strategy to mitigate the harmful impacts of insecticides on honeybees, thereby promoting their overall health and contributing to the preservation of pollination services and apiculture. Further research is warranted to elucidate the underlying mechanisms and optimize the application of these natural products in honeybee management practices.

Efficacy of botanicals against red pumpkin beetle and their impact on pollinator diversity in pumpkin (Cucurbita pepo L.) cultivation

This study aimed to assess insect pollinator diversity in pumpkin (Cucurbita pepo L.) and evaluate the bio-efficacy of botanicals for pest management while considering their impact on pollinators. Seven pollinator species were identified, with Hymenopterans, particularly honey bees, dominating. Apis dorsata (67.40%) was the most common pollinator, followed by Apis florea (14.28%). Other pollinators included species from Halictidae, Sphecidae, Syrphidae, and Pieridae families. Among the botanicals tested, Azadirachtin seed kernel extract (5%) and leaf extract (10%) effectively managed the red pumpkin beetle (Raphidopalpa foveicollis) with minimal harm to pollinators. Post-application, pollinator activity slightly increased after three to five days. The study highlights the effectiveness of neem-based botanicals in reducing pest populations while conserving pollinators, emphasizing the value of eco-friendly pest control in promoting sustainable pumpkin farming, improving both yield and crop quality.

Antibacterial Activity of Apis mellifera Bees Honey, Garlic Extracts and Their Combinations against Salmonella in Wolayta Sodo, Southern Ethiopia

Antibacterial Activity of Apis mellifera Bees Honey, Garlic Extracts and Their Combinations against Salmonella in Wolayta Sodo, Southern Ethiopia

Increasing the Capacity of Teachers and Students in Understanding the Concept of Environmental Awareness of Honey Bee Cultivation

Bees are honey-producing insects from the genus apis. In addition to honey, bees also produce pollen, royal jelly, propolis, beeswax and bee venom. Honey products that are widely found in Indonesia come from local honey bees, superior bees, wild bees, and lanceng bees. The high demand has driven up the price of honey bee products. Propolis has various health benefits such as containing high antioxidants and phenols, so it has the potential to cure various diseases such as toothache, influenza, diabetes mellitus, tuberculosis, and others. The purpose of this community service is to provide assistance to students regarding environmental awareness of honey bee cultivation at the Pattimura University Laboratory Middle School, Ambon and to increase the capacity of teachers in understanding the concept of environmental awareness of honey bee cultivation. The steps taken for learning honey bee cultivation use Project based learning (PjBL).

Testing microbial pest control products in bees, a comparative study on different bee species and their interaction with two representative microorganisms

BackgroundThe evaluation of the impact of pesticides on non-target species, like bees, is a crucial factor in registration procedures. Therefore, standardized test procedures have been developed on OECD level assessing the effects of chemicals on honey bees or bumble bees. Unfortunately, these protocols cannot directly be adapted for testing products that contain microorganisms. Interest in the use of microorganisms has increased in recent years due to their specificity to target species while not harming non-target organisms. This study aimed to evaluate optimal conditions to assess the effects of microbial plant protection products on bee species according to currently available test protocols. Some of the most commonly used microorganisms for plant protection, Bacillus thuringiensis subspecies aizawai (B. t. a. ABTS 1857) and Beauveria bassiana (B. b. ATCC 74040) were tested on Apis mellifera, Bombus terrestris, and Osmia bicornis at different temperatures (18, 26, 33 °C) under laboratory conditions.ResultsExposure to the product containing B. t. a. ABTS 1857 resulted in higher mortality compared to B. b. ATCC 74040 in all tested bee species. A temperature-dependent effect towards higher mortality at higher temperatures of 26 °C or 33 °C was observed in O. bicornis exposed to both microorganisms. A. mellifera showed variable responses, but for B. terrestris there was mostly no effect of temperature when exposed to microorganisms in high concentrations. However, temperature affected longevity of bee species in the non-exposed control group. A. mellifera mortality increased with decreasing temperatures, while B. terrestris and O. bicornis mortality increased with increasing temperatures. A test duration of 15 or 20 days was found to be suitable for testing these microorganisms.ConclusionIn conclusion, 26 °C should be considered the worst-case scenario for testing B. bassiana on all tested bee species. For testing B. thuringiensis, a temperature of 33 °C is recommended for A. mellifera, whereas B. terrestris and O. bicornis should be tested at 26 °C.

Transcriptional responses of detoxification genes to coumaphos in a nontarget species, Galleria mellonella (greater wax moth) (Lepidoptera: Pyralidae), in the beehive environment

The greater wax moth Galleria mellonella is a cosmopolitan pest of hives of the western honey bee Apis mellifera, where it remains exposed to varroicides applied by beekeepers in past decades as pest management chemicals for control of Varroa destructor, a devastating ectoparasite of bees. The prolonged presence of coumaphos residues, an organophosphate varroicide, in beeswax may be responsible for current levels of tolerance exhibited by G. mellonella, a non-target species that infests beehives. In this study, a field-collected strain of waxworms exhibited a higher LC50 value for coumaphos than that of a laboratory strain that had not been continuously exposed to coumaphos residues at field concentrations. Despite its higher tolerance for coumaphos, the field strain experienced growth inhibition at ecologically relevant concentration of coumaphos. Moreover, at low environmental concentrations that did not alter growth, detoxification gene expression levels were substantially altered. RNA-Seq analysis revealed 1181 and 658 differentially expressed genes in fat body and midgut, respectively, with 378 and 186 of those genes upregulated. This large-scale upregulation encompassed 21 genes encoding cytochrome P450 monooxygenases (CYPs), 13 encoding UDP-glycosyltransferases (UGTs), 5 encoding glutathione-S-transferases (GSTs), 2 encoding carboxylesterases (COEs), and 2 encoding ABC transporters (ABCs) in either tissue. Expression analysis of 13 selected candidate detoxification genes by RT-qPCR was consistent with their expression from RNA-Seq data. In sum, our results indicate that long-lasting pesticide residues in beeswax from past Varroa mite management may continue to act as selective agents on detoxification systems of hive residents other than the initial target species and that multiple resistance mechanisms to these chemicals may coexist within the beehive fauna.

Assessing Queen Cell Cups Priming from Combs of Different Honey Bee Species for Mass Rearing of Apis mellifera L. Queen Bee

Experiments were carried out at the Beekeeping-cum-Honey Production Unit, Department of Entomology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India, during 2020-21. The study was aimed to evaluate queen cell cups prepared from combs of different bee species to find out a suitable technique for royal jelly production through mass queen bee rearing of Apis mellifera species. The worker bees of Apis mellifera (AM) exhibited a low preference for working with combs produced by Apis dorsata (AD) and Apis cerana (AC) for queen cell construction amongst evaluated combs. In contrast, when queen cell cups were made using AM combs, the worker bees attempted to build 100% of the cells. The time required by AM worker bees to complete queen cell construction on AM combs ranged from 7 to 15 days, however, they took 1.20 and 0.98 times longer to complete cells made with AD and AC combs, respectively. Similarly, queen bees showed reluctance to lay eggs in cells built on AD and AC combs. In queen cells constructed on AM combs, the queen bee began laying eggs on the second day after release into the nucleus hive and continued for five days, with an increasing trend in egg-laying activity. Among the different batches of eggs laid, the third batch was the most successful in respect of the larval growth and development. Additionally, larval growth showed a consistent upward trend during the first three days of observation for each laying batch. A. mellifera combs have been found to be more favourable for queen cell construction by worker bees, showing a strong preference for the queen's egg-laying performance, as well as optimal larval growth and development. Based on these findings, it is recommended that beekeepers may adopt this method to promote mass queen bee rearing and enhance royal jelly production.

Do diet and Fumagillin treatment impact Vairimorpha (Nosema) spp. (Microspora: Nosematidae) infections in honey bees (Hymenoptera: Apidae) and improve survival and growth of colonies overwintered in cold storage?

Vairimorpha (Microsporidia: Nosematidae)is a microsporidian that infects honey bees especially in winter. Fumagillin can reduce infections, but whether overwintering survival is improved is unclear. The diet also may influence the severity of Nosema infections. We examined the relationship between Nosema and colony size and survival in hives overwintered in cold storage facilities. In year 1, no Fumagillin treatments were applied. Colony size and survival after cold storage and almond bloom were comparable between groups with high and low pre-cold storage infections. In year 2, size and survival were compared among colonies with and without Fumagillin treatment that were fed either pollen or protein supplement prior to overwintering. Colonies treated with Fumagillin had lower spore numbers than untreated, but colony sizes and survival were similar among the treatments. However, more colonies with zero spores per bee could be rented for almond pollination and were alive after bloom than those averaging >1 million spores per bee. Fat body metrics can affect overwintering success. In both years, fat body weights and protein concentrations increased, and lipid concentrations decreased while bees were in cold storage. Fat body metrics did not differ with Nosema infection levels. However, Fumagillin negatively affected pre-cold storage fat body protein concentrations and colony sizes after cold storage and almond bloom. Treating with Fumagillin before overwintering in cold storage might result in greater colony survival if spore numbers are high, but undetectable or even negative effects when spore numbers are low.

New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph

Honey bees are rapidly declining, which poses a significant threat to our environment and agriculture industry. These vital insects face a disease complex believed to be caused by a combination of parasites, viruses, pesticides, and nutritional deficiencies. However, the real aetiology is still enigmatic. Due to the conventional analysis methods, we still lack complete insights into the honey bee virome and the presence of pathogenic bacteria. To fill this knowledge gap, we employed third-generation nanopore metagenomic sequencing on honey bee haemolymph to monitor the presence of pathogens over almost a year. This study provides valuable insights into the changes in bacterial and viral loads within honey bee colonies. We identified different pathogens in the honey bee haemolymph, which are not included in honey bee screenings. These pathogens comprise the Apis mellifera filamentous virus, Apis rhabdoviruses, and various bacteria such as Frischella sp. and Arsenophonus sp. Furthermore, a sharp contrast was observed between young and old bees. Our research proposes that transgenerational immune priming may play a role in shaping infection patterns in honey bees. We observed a significant increase in pathogen loads in the spring, followed by a notable decrease in pathogen presence during the summer and autumn months. However, certain pathogens seem to be able to evade this priming effect, making them particularly intriguing as potential factors contributing to mortality. In the future, we aim to expand our research on honey bee transgenerational immune priming and investigate its potential in natural settings. This knowledge will ultimately enhance honey bee health and decrease colony mortality.

The selection traits of mite non-reproduction (MNR) and Varroa sensitive hygiene (VSH) show high variance in subsequent generations and require intensive time investment to evaluate

The honey bee ectoparasite Varroa destructor is the main cause of honey bee colony losses worldwide. Over the last decades, several projects have focused on improving the robustness of Apis mellifera against this parasitic mite. Selection traits, such as mite non-reproduction (MNR) and Varroa sensitive hygiene (VSH), are favored selection factors in Varroa resistance projects. VSH is a trait where adult honey bees remove the Varroa-infested brood. During this process, the female mites are arrested in their reproductive cycle leading to a reduction of the Varroa population within the bee colony. From 2019 to 2022, 1402 queens were instrumentally inseminated with single or multiple drones in a breeding program. Colonies headed by these queens were established annually, and the MNR and VSH levels were analyzed. VSH was evaluated in response to cells artificially infested with Varroa, and colonies with high VSH values were used to generate our selected VSH stock. Despite crossing high VSH drones and queens, we measured a remarkable heterogeneity of MNR and VSH in the next generation(s), most likely due to the well-described, high recombination rate in the honey bee genome. When assessed multiple times in the same colony, great variance between measurements was observed. Detailed evaluations of daughter colonies are thus required if selection programs want to breed colonies with reliable VSH traits. This constant need to evaluate all offspring to ensure the desirable resistance traits are present results in high workloads and great expenses in selection programs. Furthermore, such large-scale breeding programs are inefficient due to high fluctuations between measurements and generations, indicating we need to develop new approaches and improved methods for assessing Varroa resistance.

Is it me or is it you? Physiological effects of the honey bee microbiota may instead be due to host maturation.

Microbiota-mediated impacts on host physiology and behavior have been widely reported in honey bees (Apis mellifera). However, most of these studies are conducted in artificial lab settings and fail to take into account, or make incorrect assumptions about, the complex physical and social structures inherent to natural hive conditions. A new study by Liberti et al. (J. Liberti, E. T. Frank, T. Kay, L. Kesner, et al., mBio 15:e01034-24, 2024, https://doi.org/10.1128/mbio.01034-24) identifies one such overlooked aspect-the behavioral maturation from nurses to foragers-that can be a serious confounding factor in bee microbiota experiments. Using cuticular hydrocarbon profiling to discern between the two maturation states, they find that multiple physiological and behavioral differences between age-matched lab bees could potentially be explained by their maturation state instead of the intended treatment conditions, such as microbial inoculation. This study serves as a stark wake-up call on the necessity of careful replication and cross-disciplinary knowledge transfer (e.g., between animal specialists and microbiologists) in order to truly understand complex host-microbe systems.