Populus Euphratica Research Articles

Spatial distribution patterns of trunk internal decay of Euphrates poplar riparian forest along the Tarim River, northwest China

Trunk internal decay is a common phenomenon in forest ecosystems, causing the loss of forest resources. Euphrates poplar (Populus euphratica Oliv.) is the only constructive tree species of the desert riparian forests along the Tarim River in northwestern China. The ecosystem stability of these forests has been damaged due to widespread internal decay or hollow rot in many tree trunks. However, forest resource conservation is difficult due to the unclear spatial patterns of P. euphratica trunk decay under different water conditions and tree morphology in the middle (MTR) and lower reaches (LTR) of the Tarim River. We conducted a field experiment and lab analysis to assess the reliability of the ARBOTOM® 2D stress wave Non-Destructive Testing (NDT) technique on P. euphratica populations. We explored the quantitative characterization method and the spatial distribution patterns of P. euphratica trunk internal decay in the MTR and LTR. Our study found that (1) the accuracy of the stress wave NDT technique in detecting trunk decay of P. euphratica in the arid desert area was more than 80%; The severity of internal trunk decays can be quantitatively characterized by the combination of the Matlab Image Processing Toolbox, (2) the trunk decay proportion of P. euphratica showed an increasing trend with the tree height in the MTR. The LTR first increased, then decreased. The degree of decay was highest when the tree height was 6–7 m. The trunk decay proportion of individual trees positively correlated with the diameter at breast height and crown loss (p < 0.001) and negatively correlated with the crown diameter (p < 0.001). The higher the decay occurrence, the worse the individual size was, and vice versa, and (3) the decay proportion of the P. euphratica showed an overall increasing trend with an increase in groundwater depth. The trunk decay was significantly higher in the LTR compared to the MTR at the same distance of 300–1050 m from the river channel. Our findings suggested that the trunk decay in P. euphratica had a higher difference among different tree morphology, and the occurrence of decay in the LTR was much higher than in the MTR. The water stress at the site is one of the leading factors in internal defects in the riparian forests along the Tarim River. The results provide a theoretical basis and scientific support for desert riparian forest ecosystems’ conservation and sustainable management.

Coupling the water use of Populus euphratica and Tamarix ramosissima and evapotranspiration partitioning in a desert riparian forest ecosystem

Evapotranspiration (ET) partitioning involves determining how the flux of water is mediated by abiotic and biotic pathways. Previous studies have shown the average transpiration fraction (T/ET) from sites to global scales. However, few studies couple plant water use with ET partitioning to understanding how changes in plant community structure affect the seasonal to interannual variability in ET partitioning in desert riparian forest ecosystem. In this study, we used an isotope tracer coupled to a ''multi-source'' model to estimate the ET and its components, such as soil evaporation (E) and plant transpiration (T). The total T was further partitioned into the transpiration of Populus euphratica (Tp) and Tamarix ramosissima (Tt). The consistency of R2 (0.78 and 0.74) between the simulated and total ET measured by eddy covariance and Tp by sap flow illustrated that this model can reconstruct the water exchange in desert riparian forest ecosystem. The fraction Tp/ET was slightly higher than Tt/ET, but less than E/ET, and their ratios were 0.16, 0.14 and 0.70, respectively, from 2014 to 2017. The fractions of Tp/T and Tt/T were almost equal values of 52% and 48%. The different water use strategies and stomatal conductance sensitivities between P. euphratica and T. ramosissima demonstrated that their different responses to the changes of vapor pressure deficit (VPD) and groundwater depth. In drought period, Tp was greater than Tt, indicating that P. euphratica had a competitive advantage because of its deep groundwater utilization strategy and high sensitivity to stomatal conductance. The change of community structure indicated by increase coverage of P. euphratica in desert riparian forest ecosystem had significant enhance on T/ET than total ET. This study emphasizes that considering the different water uses among various species to optimize the ecological water demand will improve the management of desert riparian forest ecosystems.

Overexpression of PeNAC122 gene promotes wood formation and tolerance to osmotic stress in poplars

Finding the adequate balance between wood formation and abiotic stress resistance is still an important challenge for industrial woody crops. In this study, PeNAC122, a member of the NAC transcription factor (TF) family highly expressed in xylem, was cloned from Populus euphratica. Tissue expression and β-glucuronidase (GUS) staining showed that PeNAC122 was exclusively expressed in phloem fiber and secondary xylem of stems. Subcellular and yeast transactivation assays confirmed that PeNAC122 protein existed in the nucleus and did not have transcriptional activation and inhibitory activity. Overexpression of PeNAC122 poplar lines exhibited reduced plant height, thickened xylem, and accumulated lignin content in stems, and also upregulates the expression of secondary cell wall biosynthetic genes. Moreover, overexpression of PeNAC122 lines displayed more tolerance to PEG6000-induced osmotic stress, with stronger photosynthetic performance, higher antioxidant enzyme activity, and less accumulation of reactive oxygen species in leaves, and higher expression levels of stress response genes DREB2A, RD29, and NCED3. These results indicate that PeNAC122 plays a crucial role in wood formation and abiotic stress tolerance, which, in addition to potential use in improving wood quality, provides further insight into the role of NAC family TFs in balancing wood development and abiotic stress resistance.

Resistance and Resilience of Desert Riparian Communities to Extreme Droughts

Changes in the resistance, recovery, and resilience of three desert riparian ecosystems to extreme droughts and the influence of ecological characteristics on them from 2000 to 2020 were analyzed within the Tarim River Basin, Xinjiang, China. The results showed that: (1) The resistance of the Populus euphratica forest community was the highest among the three plant communities. The recovery and resilience of the shrub community were the highest; (2) the resilience increased the most for the shrub community and decreased slightly for the Populus euphratica forest and herb communities as hydrological droughts waned; (3) the relative height and density significantly increased for Populus euphratica, decreased for herbs, and did not change for shrubs from 2000 to 2020; (4) the diversity indexes did not significantly change for the Populus euphratica forest and herb communities, and they increased for the shrub community from 2000 to 2020; (5) the resistance and resilience of the Populus euphratica forest, shrub, and herb communities were significantly affected by ecological characteristics, especially the Margalef richness index; and (6) the shrub community is more likely to rapidly recover (that is an increase of cover, height, and density) from future droughts in the Tarim River Basin, particularly with the implementation of new and more effective ecological restoration projects.

Sex‐specific strategies of nutrient resorption associated with leaf economics in Populus euphratica

Abstract There are differences between sexes in physiological and functional traits and possibly in nutrient resorption, particularly in nutrient‐poor environments. However, little is known about the extent of nutrient resorption from fine stems and fine roots, and about how nutrient resorption is related to leaf economics in the males and females of dioecious trees. We investigated nutrient resorption of different organs and explored whether nutrient resorption is associated with nutrient conservation traits of leaves (e.g. leaf thickness, leaf mass per area, LMA) in Populus euphratica females and males in four natural forests along the Tarim River, China. Both female and male leaves had the highest N resorption efficiency (NRE), than stems and roots. We found sexual dimorphism in leaf nutrient resorption at Shaya, Luntai and Yuli forest sites, where P. euphratica males had higher leaf NRE than females, whereas females had a higher leaf P resorption efficiency (PRE). Moreover, the different nutrient resorption strategies were related to leaf economics. P. euphratica males possess a conservation strategy with a higher leaf thickness, LMA and leaf vein density, which positively correlated with leaf NRE, while females with higher leaf PRE resorbed disproportionately more P for the reproductive investment. Synthesis. Populus euphratica males possess a conservation strategy with higher leaf NRE, while females have higher leaf PRE for the reproductive investment. Due to spatial sexual segregation across environmental gradients, dioecious plants are especially vulnerable under future climate change. The differences in nutrient uptake and utilization strategies between females and males may result in a situation where one sex is more prone to future climate change than the other one. Such sex‐specific nutrient resorption strategies are associated with leaf economics. The present study deepens our understanding of the nutrient balance and adaptation strategies of plants under climate change.

PeTGA1 enhances disease resistance against Colletotrichum gloeosporioides through directly regulating PeSARD1 in poplar

Basic leucine zipper (bZIP) proteins play important roles in responding to biotic and abiotic stresses in plants. However, the molecular mechanisms of plant resistance to pathogens remain largely unclear in poplar. The present study isolated a TGACG-binding (TGA) transcription factor, PeTGA1, from Populus euphratica. PeTGA1 belongs to subgroup D of the bZIP family and was localized to the nucleus. To study the role PeTGA1 plays in response to Colletotrichum gloeosporioides, transgenic triploid white poplars overexpressing PeTGA1 were generated. Results showed that poplars with overexpressed PeTGA1 showed a higher effective defense response to C. gloeosporioides than the wild-type plants. A yeast one-hybrid assay and an electrophoretic mobility shift assay revealed that PeTGA1 could directly bind to the PeSARD1 (P. euphratica SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1) promoter, an important regulator for salicylic acid biosynthesis. The transactivation assays indicated that PeTGA1 activated the expression of PeSARD1, and PR1 (PATHOGENESIS-RELATED 1), a SA marker gene involved in SA signaling. Subsequently, we observed that the PeTGA1 overexpression lines showed elevated SA levels, thereby resulting in the increased resistance to C. gloeosporioides. Taken together, our results indicated that PeTGA1 may exert a key role in plant immunity not only by targeting PeSARD1 thus participating in the SA biosynthesis pathway but also by involving in SA signaling via activating the expression of PR1.

Population Structure and Spatial Distribution Pattern of Populus euphratica Riparian Forest Under Environmental Heterogeneity Along the Tarim River, Northwest China.

Populus euphratica Oliv. (Euphrates poplar), as the dominant tree species of desert riparian forests along the Central Asian inland rivers, plays a critical role in protecting arid land ecosystems. In recent decades, climate change and excessive water resources utilization activities have led to the environmental degradation of desert riparian forests along the Tarim River in northwest China. Understanding the forest stand structure and spatial distribution pattern provide important guidance for monitoring forest dynamics in support of sustainable management. However, few studies have examined how riparian forests stand attributes differ in response to environmental heterogeneity. In this study, terrestrial laser scanning (TLS) was applied to acquire a total of 1648 individual P. euphratica tree’s 3D structure attributes within 18 plots along the upper, middle, and lower reaches of the Tarim River, which included tree height (TH), diameter at breast height (DBH), crown diameter (CD), crown projection area (CPA), stand density index (SDI), age structure ratios, and spatial pattern. The results showed that the average tree segmentation and structure determination accuracies of TLS were 93.2 and 94.6%. From the upper to the lower reaches, the average TH and CD decreased by 3.8 and 0.3 m, while the DBH increased by 4.2 cm. The SDI and CPA exhibited the following order: upper reaches (454 n ha–1, 82.3%) > middle reaches (382 n ha–1, 67.3%) > lower reaches (263 n ha–1, 39.1%), the differences were significant at 0.05 level. The population age structure changed from growing population in the upper reaches to stable population in the middle and a temporarily stable population in the lower reaches. The pair correlation g(r) function determined random distribution pattern in the upper reaches [g(r) = 1.2], an aggregated pattern in the middle [g(r) = 3.1], and lower reaches [g(r) = 9.7]. The decline in groundwater depth and soil moisture increased aggregated distribution pattern (R = 0.67 and 0.56, P < 0.05) of the P. euphratica along the mainstream of Tarim River. The results enrich our understanding of the current development stage of P. euphratica, which is important for optimizing management strategies and realizing the sustainability of floodplain ecosystems.

Genetic Architecture of Heterophylly: Single and Multi-Leaf Genome-Wide Association Mapping in Populus euphratica.

Heterophylly is an adaptive strategy used by some plants in response to environmental changes. Due to the lack of representative plants with typical heteromorphic leaves, little is known about the genetic architecture of heterophylly in plants and the genes underlying its control. Here, we investigated the genetic characteristics underlying changes in leaf shape based on the model species, Populus euphratica, which exhibits typical heterophylly. A set of 401,571 single-nucleotide polymorphisms (SNPs) derived from whole-genome sequencing of 860 genotypes were associated with nine leaf traits, which were related to descriptive and shape data using single- and multi-leaf genome-wide association studies (GWAS). Multi-leaf GWAS allows for a more comprehensive understanding of the genetic architecture of heterophylly by considering multiple leaves simultaneously. The single-leaf GWAS detected 140 significant SNPs, whereas the multi-leaf GWAS detected 200 SNP-trait associations. Markers were found across 19 chromosomes, and 21 unique genes were implicated in traits and serve as potential targets for selection. Our results provide novel insights into the genomic architecture of heterophylly, and provide candidate genes for breeding or engineering P. euphratica. Our observations also improve understanding of the intrinsic mechanisms of plant growth, evolution, and adaptation in response to climate change.

Whole-Plant Water Use and Hydraulics of Populus euphratica and Tamarix ramosissima Seedlings in Adaption to Groundwater Variation

Riparian phreatophytes in hyperarid areas face selection pressure from limiting groundwater availability and high transpiration demand. We examined whole-plant water use and hydraulic traits in Populus euphratica and Tamarix ramosissima seedlings to understand how they adapt to groundwater variations. These species coexist in the Tarim River floodplain of western China. Measurements were performed on 3-year-old seedlings grown in lysimeters simulating various groundwater depths. P. euphratica had relatively greater leaf area-specific water use due to its comparatively higher sapwood area to leaf area ratio (Hv). A high Hv indicates that its sapwood has a limited capacity to support its leaf area. P. euphratica also showed significantly higher leaf-specific conductivity (ksl) than T. ramosissima but both had similar sapwood-specific conductivities (kss). Therefore, it was Hv rather than kss which accounted for the interspecific difference in ksl. When groundwater was not directly available, ksl and Hv in P. euphratica were increased. This response favors water loss control, but limits plant growth. In contrast, T. ramosissima is more capable of using deep groundwater. Stomatal sensitivity to increasing leaf-to-area vapor pressure deficit was also higher in P. euphratica. Overall, P. euphratica is less effective than T. ramosissima at compensating for transpirational water loss at a whole-plant level. For this reason, P. euphratica is restricted to riverbanks, whereas T. ramosissima occurs over a wide range of groundwater depths.

High-throughput sequencing reveals rhizosphere fungal community composition and diversity at different growth stages of Populus euphratica in the lower reaches of the Tarim River.

BackgroundPopulus euphratica is one of the most ancient and primitive tree species of Populus spp and plays an important role in maintaining the ecological balance in desert areas. To decipher the diversity, community structure, and relationship between rhizosphere fungi and environmental factors at different growth stages of P. euphratica demands an in-depth investigation.MethodsIn this study, P. euphratica at different growth stages (young, medium, overripe, and decline periods) was selected as the research object, based on the determination of the physicochemical properties of its rhizosphere soil, the fungal community structure and diversity of P. euphratica and their correlation with soil physicochemical properties were comprehensively analyzed through high-throughput sequencing technology (internal transcribed spacer (ITS)) and bioinformatics analysis methods.ResultsAccording to the analysis of OTU annotation results, the rhizosphere soil fungal communities identified in Populus euphratica were categorized into10 phyla, 36 classes, 77 orders, 165 families, 275 genera and 353 species. The alpha diversity analysis showed that there was no obvious change between the different growth stages, while beta diversity analysis showed that there were significantly differences in the composition of rhizosphere soil fungal communities between mature and overripe trees (R2 = 0.31, P = 0.001), mature and deadwood (R2 = 0.28, P = 0.001). Ascomycota and Basidiomycota were dominant phyla in the rhizosphere fungal community and the dominant genera were Geopora, Chondrostereum and unidentified_Sordariales_sp. The relative abundance of the top ten fungi at each classification level differed greatly in different stages. Canonical correspondence analysis (CCA) and Spearman’s correlation analysis showed that conductivity (EC) was the main soil factor affecting the composition of Populus euphratica rhizosphere soil fungal community (P < 0.01), followed by total dissolvable salts (TDS) and available potassium (AK) (P < 0.05).ConclusionsOur data revealed that the rhizosphere fungal communities at the different growth stages of P. euphratica have differences, conductivity (EC) was the key factor driving rhizosphere fungi diversity and community structure, followed by total dissolvable salts (TDS) and available potassium (AK).

Assessment of Tolerance Levels to Air Pollution for Some Species of Trees and Shrubs Growing on Roadsides in Western Mosul City

This study was conducted in the western part of Mosul city from 20/9/2020 to the end of spring 2021 to assess the tolerance of some trees and shrubs against air pollution based on the air pollution tolerance index APTI. The sites were tested according to the different traffic density and human activities, and included areas Residential, main and secondary roads, samples of healthy leaves were collected for 19 species of trees and shrubs in the season of summer, winter, spring and autumn, and included some evergreen species such as orientalis Biota, , Nerium oleander , Cupressus sempervirens var pyramidalis , Cupressus sempervirens var.horizantalis, Casuarina equistifolia , Olea europaea , Eucalyptus camaldulenses , Pinus brutia , Dypsis lutesens , Washingtonia filifera , Tamarix articulate , Callistemon viminalis , Leucaena leucephala, And falling leaves Melia azedarach , Morus alba , Ziziphus spina-christi , Ficus carica , Albizia lebbeek ,Populus euphratica. . Some biochemical characteristics were used to evaluate the ability of these species to resist pollutants and the possibility of using them for planting on the roadsides. Analysis of variance and LSD test were used to find out the statistical differences between the different species. The results of this study indicated that there was a significant difference between the different species, as a result, the highest value of the relative moisture content of the callistemon viminalis trees in the Al tayaran area was (87.461%) among all other plant species, the Leucaena leucephala trees contained a high percentage of chlorophyll,

Economically valuable species growing in Northern Kyzylkum and their significance for practical use

Unique genetic resources of plant biodiversity are concentrated in Kazakhstan. Economically important species make a significant contribution to the structure of the Earth in comparison with taxa with insignificant or unknown economic use, many economically valuable species of Kazakhstan are of global importance. The purpose of this work is to identify economically valuable species of Northern Kyzylkum within the Republic of Kazakhstan. Objects and methods of research: To collect and identify species, classical botanical, route-reconnaissance; ecological-systematic; ecological-geographical methods were used. The main groups of useful plants of economically valuable flora species of Northern Kyzylkum were identified: woody, food, medicinal, fodder, decorative, technical species. During the research, it was revealed that 310 economically valuable species belonging to 62 families and 196 genera are found in Northern Kyzylkum within the Republic of Kazakhstan. According to the results of the classification, the dominant families are determined: Poaceae Barnhart has 34 genera, 58 species; Asteraceae Dumortier – 24 genera, 46 species; Chenopodiaceae Vent. – 19 genera, 30 species; Fabaceae Juss. – 11 genera, 23 species. The study analyzed 200 types of medicinal, 100 types of fodder, 22 types of decorative, 11 types of sand fixers, 9 types used for fuel according to significant economic valuable characteristics. According to the classification of economically valuable species by life forms, tree forms were identified in 6 species: Salix alba L., Populus diversifolia Schrenk, P. pruinosa Schrenk, Acer negundo L., Elaeagnus angustifolia L., Haloxylon persicum Bunge ex Boiss., 44 shrubs and semi-shrubs, 155 perennials, 105 biennial and annual plants. Key words: economically-valuable species, Northern Kyzylkum, fodder, medicinal, useful, decorative.

Comparative Analysis of the Endophytic Bacterial Diversity of Populus euphratica Oliv. in Environments of Different Salinity Intensities.

ABSTRACTPopulus euphratica Oliv. has a high tolerance for drought, salinity, and alkalinity. The main purpose of this study is to explore the effects of environments of different salinity intensities on endophytic community structure and the possible roles of endophytes in the tolerance of host plants. The characterization of endogenous bacteria in diversity has been investigated by using the Illumina high-throughput sequencing technique. The research showed that endophytic bacteria of P. euphratica in an extremely saline environment had low species diversity, especially in sap tissue. The dominant phyla in all groups were Proteobacteria, Actinobacteria, and Bacteroidetes. Notably, Firmicutes (relative abundance >5%) was a different dominant phylum in the samples from the high-saline environment compared with the relatively low-saline-environment group. The linear discriminant analysis effect size (LEfSe) analysis found that there were significant differences in different saline environments of Cytophagaceae (family), Rhodobacteraceae (family), and Rhodobacterales (order). These results indicated that the composition of the endogenous bacterial community was related to the growth environment of host plants. The predictive analysis of KEGG pathways and enzymes showed that the abundance of some enzymes and metabolic pathways of endophytes of P. euphratica increased with the increase of soil salinity, and most of the enzymes were related to energy metabolism and carbohydrate metabolism. These findings suggested that the endogenous bacteria of the host plant had different expression mechanisms under different degrees of stress, and this mechanism was very obvious in the distribution of endophytes, while the function of the endogenous bacteria needs to be further explored.IMPORTANCE Euphrates poplar (Populus euphratica Oliv.), as the only tree species that grows in the desert, has tenacious vitality with the characteristics of cold tolerance, drought tolerance, salt-alkali tolerance, and wind-sand resistance. P. euphratica has a long growth cycle and a high growth rate, which can break wind, fix sand, green the environment, and protect farmland, making it an important afforestation tree species in arid and semiarid areas. The area of P. euphratica in Xinjiang accounts for 91.1% of its area in China. Studying the endophytic bacteria of P. euphratica can give people a systematic understanding of it and the adaptability of the endogenous flora to the host and special environments. In this study, by analyzing the endophytic bacteria of P. euphratica in different saline-alkali regions of Xinjiang, it was found that the bacteria in different tissues of P. euphratica changed with the change of soil salinity. Especially in the sap tissue of P. euphratica under extremely high salinity, the diversity of endogenous bacteria was significantly lower than that in other tissues. These differential bacteria under different salinities were mostly related to the stress resistance of themselves and the host. Not only that, we also selected a strain of Bacillus with high stress resistance from the tissues of P. euphratica, which can survive under the extreme conditions of 10% NaCl and pH 11. We obtained a genome completion map of this strain, named it Bacillus haynesii P19 (GenBank accession no. PRJNA648288), and tried to use it for fermentation but in a different work, so as to develop it into a promising industrial fermentation chassis bacterium. Therefore, this study was of great significance for the understanding of endophytic bacteria in P. euphratica and the acquisition of extremophilic microbial resources.

Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K+/Na+ and ROS Homeostasis in Arabidopsis.

Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H2O2 levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na+/H+ antiporter (AtSOS1) and H+-ATPase (AtAHA2), which enabled transgenic plants to proceed with Na+ extrusion and reduce K+ loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K+/Na+ homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K+/Na+ homeostasis in Arabidopsis.

A method to determine optimum ecological groundwater table depth in semi-arid areas

Groundwater depth (GWD) is an important factor to sustain the ecological integrity of some ecosystems and is often used as an indicator of environmental quality in dry areas. Single-scale data gained from quadrat surveys is always used to establish a relationship with GWD to determine the optimum GWD. However, the randomness and uncertainty in single-scale data may result in insufficient reliability of results. To overcome this shortage, multiple growth indicators of poplar trees (Populus euphratica) in Hetao Irrigation District, including average crown width (ACW), tree height, diameter at breast height (DBH), mean ring spacing (MRC), and normalized difference vegetation index (NDVI), were acquired by field sampling and remote sensing. These indicators were used to establish relationships with the GWD by considering spatial and temporal variation to identify the optimum GWD. The cloud model was introduced and its three digital features derived from optimum groundwater depth data (expectation: Ex, entropy: En, and super-entropy: He) were calculated to construct the reverse cloud models W (Ex, En, He) for describing ecological GWD to determine the optimum ecological GWD in semi-arid areas. The results show that the optimum GWD range was 1.60–2.20 m. The cloud models obtained on spatial and temporal scales were WS (2.01, 0.07, 0.04) and WT (1.78, 0.10, 0.02), respectively. The resulting comprehensive cloud model WC (1.87, 0.14, 0.03) exhibited better variability, so 1.87 m was taken as the optimum GWD for poplars. This method can determine the regional ecological groundwater level more accurately and effectively, and provide evaluation indicators for the management of regional groundwater.

Water Resource Management Implications for a Desert Oasis From Tree‐Ring δ18O Variations in Populus Euphratica in Northwest China

AbstractPaleoclimatic data has often been applied to demonstrate the influence of anthropogenic activities on runoff to desert rivers, and such features as reservoirs are clearly known to influence available water in downstream riparian settings. The impact of human activities on the hydrology of a downstream desert oasis is, however, an open question. High‐resolution hydroclimate paleo‐reconstructions for desert oases are lacking, partly because paleoclimate proxies in extremely dry deserts are insensitive to extremely low precipitation. Here, we first attempt to reconstruct regional precipitation (r = −0.743, p &lt; 0.001, n = 49) for the interval 1886–2009 from the stable oxygen isotope ratio (δ18O) of five Populus euphratica trees growing in the Ejina Oasis, on the lower reaches of the Heihe River in extremely arid Northwest China. The δ18O series for P. euphratica in the oasis (∼900 m a.s.l) abruptly increases relative to an δ18O series of Qinghai spruce (Picea crassifolia) growing in upper limit of forest (∼3,000 m a.s.l) in Longshou Mountain (middle reaches of the Heihe River) after about 2000, when the Ecological Water Diversion Project on the Heihe River was implemented. This finding implies that the Ejina Oasis suffered from serious drought during the last decade, or at least that the Oasis is stressed more than expected by current climate conditions. Other evidence also indicates that human activities contributed to a decrease in air moisture in the Ejina Oasis after 2000. To mitigate water stress on the oasis, we recommend some practical measures to ensure the rational development of the desert oasis.

What Is the Effect of Quantitative Inversion of Photosynthetic Pigment Content in Populus euphratica Oliv. Individual Tree Canopy Based on Multispectral UAV Images?

It is highly necessary to apply unmanned aerial vehicle (UAV) remote sensing technology to forest health assessment. To prove the feasibility of quantitative inversion of photosynthetic pigment content (PPC) in Populus euphratica Oliv. individual tree canopy (PeITC) by using multispectral UAV images, in this study, Parrot Sequoia+ multispectral UAV system was manipulated to collect the images of Populus euphratica (Populus euphratica Oliv.) sample plots in Daliyabuyi Oasis from 2019 to 2020, and the canopy PPCs of five Populus euphratica sample trees per plot were determined in six plots. The Populus euphratica crown regions were extracted by grey wolf optimizer-OTSU (GWO-OTSU) multithreshold segmentation algorithm from the normalized difference vegetation index (NDVI) images of Populus euphratica sample plots obtained after preprocessing, and the PeITCs were segmented by multiresolution segmentation algorithm. The mean values of 27 spectral indices in the PeITCs were calculated in each plot, and the optimal model was constructed for quantitative estimation of the PPCs in the PeITCs, then the inversion results were compared and verified based on GF-6 and ZY1-02D satellite imageries respectively. The results were as follows. (1) The average value of canopy chlorophyll content (Chl) was 2.007 mg/g, the mean value of canopy carotenoid content (Car) was 0.703 mg/g. The coefficient of variation (C.V) of both were basically the same and they were both of strong variability. The measured PPCs of the PeITCs in Daliyabuyi Oasis was generally low. The average contents of chlorophyll and carotenoid in PeITC in June were more than twice those in August, while the mean ratio between them was significantly lower in June than in August. The measured PPCs had no obvious spatial distribution law. However, that could prove the rationality of sample selection in this study. (2) NDVI had the best effect of highlighting vegetation among all quadrats in the study area. Based on the GWO-OTSU multithreshold segmentation method, the canopy area of Populus euphratica could be quickly and effectively extracted from the quadrat NDVI map. The best segmentation effect of PeITCs was obtained based on a multiresolution segmentation method when the segmentation scale was 120, the shape index was 0.7, and the compactness index was 0.5. Compared with manual vectorization method of visual interpretation, the root mean square error (RMSE) and Pearson correlation coefficient (R) values of the mean NDVI values in PeITCs obtained by these two methods were 0.038 and 0.951. (3) Only 12 of the 27 spectral indices were significantly correlated with Chl and Car at the significance level of 0.02. Characteristics of the calibration set and validation set were basically consistent with those of the entire set. The classification and regression tree-decision tree (CART-DT) model performed best in the estimation of the PPCs in the PeITCs, in which, when estimating the Car, the calibration coefficient of determination (R2C) was 0.843, the calibration root mean square error (RMSEC) was 0.084, the calibration residual prediction deviation (RPDC) was 2.525, the validation coefficient of determination (R2V) was 0.670, the validation root mean square error (RMSEV) was 0.251, the validation residual prediction deviation (RPDV) was 1.741. (4) Qualitative comparison of spectral reflectance and NDVI values between GF-6 multispectral imagery and Parrot Sequoia+ multispectral image on the 172 PeITCs can show the reliability of Parrot Sequoia+ multispectral image. The comparison results of five PeITCs relative health degree judged by field vision judgment, measured SPAD value, predicted value of Chl (Chlpre), the red edge value calculated by ZY1-02D (ZY1-02Dred edge) and the Carotenoid Reflection Index 2 (CRI2) value calculated by ZY1-02D (ZY1-02DCRI2) can further prove the scientificity of inversion results to a certain extent. These results indicate that multispectral UAV images can be applied for quantitative inversion of PPC in PeITC, which could provide an indicator for the construction of a Populus euphratica individual tree health evaluation indicator system based on UAV remote sensing technology in the next step.

Transcriptome Analysis of Populus euphratica under Salt Treatment and PeERF1 Gene Enhances Salt Tolerance in Transgenic Populus alba × Populus glandulosa.

Populus euphratica is mainly distributed in desert environments with dry and hot climate in summer and cold in winter. Compared with other poplars, P. euphratica is more resistant to salt stress. It is critical to investigate the transcriptome and molecular basis of salt tolerance in order to uncover stress-related genes. In this study, salt-tolerant treatment of P. euphratica resulted in an increase in osmo-regulatory substances and recovery of antioxidant enzymes. To improve the mining efficiency of candidate genes, the analysis combining both the transcriptome WGCNA and the former GWAS results was selected, and a range of key regulatory factors with salt resistance were found. The PeERF1 gene was highly connected in the turquoise modules with significant differences in salt stress traits, and the expression levels were significantly different in each treatment. For further functional verification of PeERF1, we obtained stable overexpression and dominant suppression transgenic lines by transforming into Populus alba × Populus glandulosa. The growth and physiological characteristics of the PeERF1 overexpressed plants were better than that of the wild type under salt stress. Transcriptome analysis of leaves of transgenic lines and WT revealed that highly enriched GO terms in DEGs were associated with stress responses, including abiotic stimuli responses, chemical responses, and oxidative stress responses. The result is helpful for in-depth analysis of the salt tolerance mechanism of poplar. This work provides important genes for poplar breeding with salt tolerance.

Transient Flooding and Soil Covering Interfere with Decomposition Dynamics of Populus euphratica Leaf Litter: Changes of Mass Loss and Stoichiometry of C, N, P, and K

Litter decomposition plays a critical role in carbon and nutrient cycling in terrestrial and aquatic ecosystems. However, the effects transient flooding and soil covering have on leaf litter decomposition remain unclear. The changes of litter mass loss and stoichiometric ratio of C:N (the ratio of carbon to nitrogen), C:K (the ratio of carbon to potassium), C:P (the ratio of carbon to phosphorus), N:P (the ratio of nitrogen to phosphorus), and N:K (the ratio of nitrogen to potassium) of fresh Populus euphratica (P. euphratica) leaves in surface, transient flooding, and soil covering treatments were studied using litterbags in a desert riparian forest in a field decomposition experiment for a period of 640 d. The results showed that there was a significant influence of disturbance type and incubation time on litter mass loss rate and stoichiometric ratios of C:N, C:K, C:P, N:P, and N:K of fresh P. euphratica leaves, but no significant influence of the interaction between disturbance type and incubation time on leaf litter mass loss. In three treatments, five sequential phases of leaf litter mass loss rate and different temporal change patterns of stoichiometric ratio were identified within 640 d. Transient flooding was shown to affect P. euphratica leaf litter mass loss phases compared to that in no-disturbance conditions, and especially promote leaf litter mass loss within 0–173 d of incubation time. It was also demonstrated that transient flooding and soil covering can influence leaf litter decomposition, which led to the leaf litter mass loss rate and the stoichiometric ratios of C:N, C:K, C:P, N:P, and N:K exhibiting varied patterns and phases in different treatments during decay.

Identification and regulatory network analysis of SPL family transcription factors in Populus euphratica Oliv. heteromorphic leaves

The SQUAMOSA promoter-binding protein-like (SPL) family play a key role in guiding the switch of plant growth from juvenile to adult phases. Populus euphratica Oliv. exhibit typical heterophylly, and is therefore an ideal model for studying leaf shape development. To investigate the role and regulated networks of SPLs in the morphogenesis of P. euphratica heteromorphic leaves. In this study, 33 P. euphratica SPL (PeuSPL) genes were identified from P. euphratica genome and transcriptome data. Phylogenetic analysis depicted the classification of these SPL genes into two subgroups. The expression profiles and regulatory networks of P. euphratica SPL genes analysis displayed that major P. euphratica SPL family members gradually increases from linear to broad-ovate leaves, and they were involved in the morphogenesis regulation, stress response, transition from vegetative to reproductive growth, photoperiod, and photosynthesis etc. 14 circRNAs, and 33 lncRNAs can promote the expression of 12 of the P. euphratica SPLs by co-decoying miR156 in heteromorphic leaf morphogenesis. However, it was found that the effect of PeuSPL2-4 and PeuSPL9 in leaf shape development was contrasting to their homologous genes of Arabidopsis. Therefore, it was suggested that the SPL family were evolutionarily conserved for regulation growth, but were varies in different plant for regulation of the organ development.