Eucerin Base Research Articles

Effect of watercress seed mucilage (Lepidium sativum L.) on the wound healing in New Zealand rabbits

Background and aims: Wound healing is one of the most important issues in medical science. Synthetic drugs have long been introduced and used to speed up the healing process of wounds, but most of them have many side effects. The present study was performed to evaluate the healing efficiency of watercress seed mucilage (Lepidium sativum L.) on wounds. Methods: In this study, the animals were randomly divided into five groups. The treatment groups were (1), (2), (3), sham (4), and control (5). The treatment groups were treated with 20%, 10%, and 5% watercress seed mucilage in the Eucerin base, respectively, and the sham was treated with Eucerin, while the control received no treatment. Then, 5 pairs of full-thickness wounds with a diameter of 7 mm were created on both sides of the rabbit’s spine. Macroscopic and wound area studies were performed on days 4, 7, and 14 after surgery. Results: According to the analysis of variance, there was a significant difference between groups 1, 2, 3, sham, and control (P=0.001), but no significant difference was observed between group 3, sham group, and control group (P=0.993). Based on these findings, at the end of the treatment period, group 1 had the highest healing rate and the lowest wound area (1.12±0.77) compared to group 2 (4.42±0.30) and 3 (7.05±0.35), as well as the sham (8.34±1.10) and control (8.50±1.90) groups. Thus, the treatment method of this group has been more effective in wound healing than that of the other treatment groups. Conclusion: It seems that watercress seed mucilage can treat wounds due to its important compounds such as flavonoids, phenols, and vitamins A and C.

Hydroalcoholic Extract of Scrophularia Striata Attenuates Hypertrophic Scar, Suppresses Collagen Synthesis, and Stimulates MMP2 and 9 Gene Expression in Rabbit Ear Model

ObjectivesHypertrophic scars (HSs) are caused by abnormal wound healing. To date, no standard treatment has been made available for HSs. Scrophularia striata has been reported to accelerate wound healing and has the potential to prevent HS formation. In this study, we investigated the anti-scarring effects of S. striata extract (SSE) in a rabbit ear model of scarring.MethodsIn this study, New Zealand white rabbit (weight 2.3-2.5 kg) were used. In the prevention phase of the study, three test groups received 5%, 10%, and 15% ointments of SSE in the Eucerin base, the fourth group received Eucerin, and the fifth group received no treatment. The samples were obtained on day 35 after wounding. In the treatment phase of the study, the test groups received an intralesional injection of SSE (5%, 10%, and 15%), the fourth group received an intralesional injection of triamcinolone, the fifth group received a solvent (injection vehicle), and the sixth group received no treatment. To evaluate the anti-scarring effects of SSE, the scar elevation index (SEI), epidermis thickness index (ETI), collagen deposition, and MMP2 and MMP9 gene expression were evaluated.ResultsA significant reduction in SEI, ETI, and collagen deposition was noted in animals treated with SSE compared with the control groups. In addition, topical SSE stimulated MMP2 and MMP9 gene expression.ConclusionThe findings of this study demonstrate the potential for SSE in the prevention and treatment of HS. SSE could be prepared as an appropriate formulation to treat wounds and prevent abnormal scarring.

Characteristics and in vitro anti skin aging activity and UV radiation protection of morin loaded in niosomes.

One of the dermatologic problems in elderly people is skin aging, which is a natural and complex biological process. Morin is a flavonoid with high radical scavenging activity as well as antityrosinase effects but its low water solubility has restricted its application. This research aimed to develop, characterize, and optimize morin niosomes composed of non-ionic surfactants, and evaluate the in vitro UV protection and antiaging effectiveness. Niosomes were prepared by the film hydration method with sorbitan monostearate (Span® 40), polyoxyethylenesorbitan monopalmitate (Tween® 40), and cholesterol. The niosomes were characterized in terms of size, zeta potential, morphology, in vitro release behavior, and drug entrapment efficiency (EE). Afterward, antiaging activity, including antityrosinase, antioxidant, intracellular reactive oxygen species (ROS) scavenging, and sun protection factor (SPF) were evaluated. The optimized niosomes appeared as unilamellar vesicles with a spherical shape, with size, zeta potential, and EE values of 6.13 ± 0.40 μm, -0.81 ± 0.32 mV, and 89.35% ± 2.80%, respectively. The noisome formulation remained stable at -4°C for 3 months. The release profiles of morin loaded in niosomes revealed the extended release over 8h and followed zero-order release kinetics. Morin-loaded niosomes exhibited no significant toxicity toward the L929 cell line. The niosome loaded with morin showed anti skin aging activity, including antityrosinase effects (IC50=13.17 ± 1.58 μg/ml), antioxidant (IC50=28.49 ± 2.05 μg/ml), and ROS scavenging activity. For 1% and 5% (w/w) morin niosomes in eucerin base cream, the SPF was 39.03 ± 1.01 and 38.15 ± 0.82, respectively, whereas the noisome-free morin cream exhibited an SPF of 4.47 ± 0.56. Morin-loaded niosome has been shown to provide sun protection and antiaging effects, suggesting that it could be used in pharmaceutical and cosmetic products.

Quantitative Determination of Vitamins A and E in Ointments Using Raman Spectroscopy

A quantitative analysis of vitamins A and E in commercial ointments containing 0.044% and 0.8% (w/w) of active pharmaceutical ingredients, respectively, was performed using partial least squares models based on FT Raman spectra. Separate calibration systems were prepared to determine the amount of vitamin A in a petrolatum base ointment and to quantify vitamins A and E in a eucerin base one. Compositions of the laboratory-prepared and commercial samples were controlled through a principal component analysis. Relative standard errors of prediction were calculated to compare the predictive ability of the obtained regression models. For vitamin A determination, these errors were found to be in the 3.8–5.0% and 5.7–5.9% ranges for the calibration and validation data sets, respectively. In the case of vitamin E modeling, these errors amounted to 3.7% and 4.4%. On the basis of elaborated models, vitamins A and E were successfully quantified in two commercial products with recoveries in the 99–104% range. The obtained data indicate that the Raman technique allows for accurate analysis of the composition of semisolid formulations in their native state, including low dose preparations.

Wound healing property of milk in full thickness wound model of rabbit

ObjectiveWound healing consists of several continuous phases in which various cells and chemical intermediates are involved. Milk as a rich source of nutritional elements has proved to have potential benefits for treatment of various diseases. The present study was designed to investigate the healing effect of low-fat cow's milk on an open skin wound model in the rabbit. MethodsThe 2%, 5%, and 10% (w/w) ointments of lyophilized powder of low-fat milk were prepared in the eucerin base and were applied twice daily in the treatment groups. Phenytoin 1% ointment was used as a standard control. The healing effect of the milk ointment (MO) was evaluated through the measurement of wound surface area, the extent of tissue tension, and the content of hydroxyproline. Histological evaluation of skin tissue specimens was also performed using hematoxylin and eosin staining. ResultsThe results showed that the healing rate in the treatment group was significantly higher than that of untreated group and eucerin group (p < 0.01). The best healing effect was seen in 5% milk ointment with the shortest healing time (15 days) and the highest tissue tension in comparison to other groups. Although the tissue hydroxyproline content in this group was slightly lower than that of the phenytoin group, this difference was not significant. Histologic, findings indicated increased collagen fibers, increased fibroblasts and an evident decrease in inflammatory cells in that group. ConclusionIt can, therefore, be concluded that low-fat cow's milk has significant beneficial effects on skin wound healing. Therefore, it may be used as a healing agent in different types of the wound in humans after certain clinical trials.

Healing Effect of Hawthorn (Crataegus pontica C. Koch) Leaf Extract in Dermal Toxicity Induced by T-2 Toxin in Rabbit

Background: T-2 toxin is a mycotoxin, exposure to which causes dermal and systemic toxicity. Crataegus pontica (a member of the Rosaceous family) is a small tree with caduceus foliage that is widely distributed throughout the western and central areas of Iran. Objectives: This study was performed to examine the healing effects of creams prepared using Crataegus pontica leaf extract on dermal toxicity induced by T-2 toxin. Methods: Iranian rabbits were used for this study. The back left flanks of the animals were shaved and to induce toxicity, a solution of 100 µg/12 µl of T-2 toxin in ethanol was applied to the skin for 2 successive days. Creams were prepared using Crataegus pontica leaf extract in a eucerin base at concentrations of 5%, 10%, and 15% (w/w). Beginning on the third day, the creams were applied to the skin lesions twice per day until complete healing occurred. The positive control group received the toxin only without treatment and the negative control group received solvent (ethanol) only. Healing was defined as a decreased wound margin, as well as treatment of the erythema and blisters. Results: Our findings indicated that wound healing occurred 15, 15, 12, 10, and 10 days after initial wounding in the negative control, eucerin, 5%, 10%, and 15% C. pontica extract groups, respectively. The most effective treatments were obtained with the creams containing 10% and 15% C. pontica extract. Histological findings confirmed wound reduction in the affected areas. Conclusions: The results obtained in this study indicate that C. pontica extract has a healing effect on dermal toxicity caused by T-2 toxin and is effective for its treatment.

Wound Healing Effects of Persian Oak (Quercus brantii) Ointment in Rats

Background: Persian oak (Quercus brantii) belongs to the Fagaceae family. It is a medicinal plant which is commonly used by the tribes in south western Iran to treat inflammatory and gastric ulcers. Objectives: The aim of this study was to evaluate the effectiveness of Persian oak ointment on dermal wound healing in rats. Materials and Methods: For making full-thickness wounds in rats, the excisional wound model was used. Five groups, each consisting of 8 rats were used. The authors studied a total of 40 wounds in five groups of rats. Each wound measured 15 mm × 15 mm. Persian oak ointment (1% and 2%, w/w) was prepared in eucerin base and applied on the wound twice a day until complete healing had achieved. The state of the wound healing was evaluated using wound closure ratio, wound contraction and re-epithelialization, tensile strength, hydroxyproline content and collagen content. Histopathological examination was also performed. Results: Persian oak 1% and 2% groups and the phenytoin group had a statistically significant higher wound closure ratio than the control group. The effects produced by Persian oak ointment showed significant healing improvement (P < 0.01) compared with the control group in parameters such as wound contraction, epithelialization period, hydroxyproline content and tensile strength. Histopathological studies also showed improvement in wound healing with Persian oak ointment. Conclusions: The present study affirms that Persian oak ointment demonstrates wound healing in animal model. Further studies are needed to demonstrate the possible effects of Persian oak ointment on wound healing in humans.

Wound healing potential of Althaea officinalis flower mucilage in rabbit full thickness wounds

ObjectiveTo evaluate and practically demonstrate the influence of Althaea officinalis flower mucilage as a plant known in Iran's and other Middle Eastern countries' traditional medicine for its wound healing properties. MethodsAnimals were divided into 6 groups of 5 cases including a non-treated group as the negative control group receiving no treatment, a group treated with eucerin as the positive control group, a phenytoin 1% group as a standard group treated topically with phenytoin 1% hand-made ointment, and treatment groups treated with hand-made Althaea officinalis flower mucilage (AFM) ointment in a eucerin base with different concentrations (5%, 10%, 15%). ResultsAmong the treatment groups, the AFM 15% ointment showed the best result. Wound healing duration was reduced by the surface application of these groups. Wound closure was completed on Days 14 and 15 in the AFM 15% ointment and phenytoin 1% groups, respectively. No significant difference was observed in healing period between these groups. ConclusionsIn conclusion, AFM 15% ointment was found to reduce wound healing time without any significant difference with the phenytoin 1% ointment. The authors suggest increased AFM effectiveness in when combined with phenytoin or other effectual plants.

Wound healing properties of quince seed mucilage: In vivo evaluation in rabbit full-thickness wound model

ObjectiveQuince seed mucilage (QSM) has been used in Iranian traditional medicine for the treatment of skin wounds and burns. Recent studies indicated that QSM accelerated wound healing. The present study was undertaken to investigate the healing efficiency of QSM formulated as 5%, 10%, and 20% creams in eucerin base with especial attention on growth factors involving in wound healing. MethodsFull thickness wounds were created in Iranian male rabbits divided into five experimental groups (n = 6), as negative control, eucerin and treatments. Negative control group did not receive any treatment. Eucerin group received topical eucerin, twice a day. Treatment groups were treated topically by creams of QSM 5%, 10% and 20% (w/w) in eucerin base, twice daily. The efficacy of treatment was evaluated based on wound contraction, haydroxyproline content, tensile strength of wound tissue. The levels of epidermal growth factor (EGF), transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF) and platelet derived growth factor (PDGF) were also determined in serum and wound fluid of tested animals. ResultsResults showed that there were statistically significant differences in wound contraction between QSM 10 and 20% creams treatments groups and control groups (P < 0.05) in most of the days. Rabbits treated with QSM 20% cream had the best results (completed healing in 13 days, higher hydroxyproline content, higher tissue resistance and higher wound fluid levels of evaluated growth factors). ConclusionWe concluded tha QSM in 10–20% concentrations have a good potential for promote wound healing thus supports its traditional use.

Topical vitamin K1 promotes repair of full thickness wound in rat.

Objectives:Application of vitamin K to the skin has been used for suppression of pigmentation and resolution of bruising. However, in rats, no study was reported on its effect regarding wound healing. Thus, the present study was designed to examine the healing effects of creams prepared from vitamin K1 on full-thickness wound in rats.Materials and Methods:For inducing full-thickness wound in rats, the excisional wound model was used. Five groups consisting of 8 rats each were used. Vitamin K cream (1% and 2%, w/w) was prepared in eucerin base and applied on the wound once a day until complete healing had occurred. Healing was defined by decreased wound margin (wound contraction), re-epithelialization, tensile strength and hydroxyproline content. Histopathological examination was also done.Results:The effects produced by the topical vitamin K showed significant (P < 0.01) healing when compared with control group in parameters such as wound contraction, epithelialization period, hydroxyproline content and tensile strength. Histopathological studies also showed improvement with vitamin K.Conclusions:Topical vitamin K demonstrates wound healing potential in full-thickness wound model.

Topical grape (Vitis vinifera) seed extract promotes repair of full thickness wound in rabbit

In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Grape (Vitis vinifera) seed extract (GSE) possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential activity of grape seed hydroalcoholic extract in wound healing in rabbits. Rabbits of either sex were subjected to a 20 × 20 mm square excision made over the skin of the back. The animals were randomly divided into seven experimental groups, as negative and positive control, eucerin and treatments. Negative control group did not receive any treatment. Positive control and eucerin groups received phenytoin cream (1%) and topical eucerin, respectively, twice a day from the beginning of experiments to complete wound closure. Treatment groups were treated topically by cream of GSE (2, 5, 10 and 70% w/w) in eucerin base, twice daily. For evaluation of the percentage of wound healing, area of the wound was measured daily. Histological studies were performed on the 7th and 15th days of treatments. After complete healing, hydroxyproline content and tensile strength measurement of tissue samples were done. Results showed that there were statistically significant differences between GSE treatments groups and eucerin animals (P < 0·05) in most of the days. Rabbits treated with 2% GSE had best results (completed healing in 13 days, higher hydroxyproline content and higher tissue resistance). We concluded that the extract of 2% GSE administered topically has a good potential to promote wound healing in wound model of rabbits.

The Study of Hypericum perforatum extract on wound healing in rabbit

Wound healing is a natural reaction to injury witch results in restoration of tissue integrity. Repair consists of four phases: inflammation, re-epithelialization, angiogenesis, fibroblast recruitment and scarring. In this study the healig effect of Hypericum perforatum extract was investigated on open wound. Hydroalcoholic extract of Hypericum P. was prepared by maceration method. Newzeland rabbits of either sexes weighting 1.6-2.5 kg were used. Method of Cross et al (1995) was used to perform excisional wound. Hairs of lower back and left flank of animal was carefully shaved and cleaned. Animal was held in standard crouching position. A template measuring 20×20 mm was placed on the skin and the outline traced using a fixed-tipped pen. Desired area was locally anasthetised with lidocaine 2%. Full thickness wound was made by excising the skin using a scalped blade and forceps. Every day the outline of each wound was traced by using a transparent plastic sheet. Treatment of wounds started from the first moments, twice daily. In other to minimize the errors, measurment was repeated three times. Then area of wounds was calculated by mm chart paper. Wound of bland group were left untreated. Second group was treated with eucerin as placebo control, in third group phenytion cream 1% was used as standard control and in other groups different concentration of hypericum extract 2%, 5%, and 10% w/w in eucerin base were adminstrated twice daily. Area of wound in the first day was considerd as 100% and area of subequent days were compared with initial day. Percentage of healing was calculared by substracting the percentage of area from 100. Healing of eucerin and phenytoin groups lasted 21 and 158 day respectively. Healing in Hypericum cream of 2%, 5% and 10% groups lasted 14, 15 and 16 days respectively. Best results obtained with 2% extract. The result of this study confirms that Hypericum cream 2% had the best wound healing effect even better than phenytion cream, however the Hypericum Cream 5% & 10% had good and noticeable effect too.

Association of Non-Healing Wounds, Pain and Neurochemical Alterations In Sickle Cell Disease

AbstractAbstract 842The mechanisms underlying non-healing leg ulcers in sickle cell disease (SCD) remain unknown and their treatment is difficult. We hypothesized that leg ulcers in SCD cause injury to nerve fibers leading to neurogenic inflammation, and peripheral and central sensitization resulting in chronic pain and impairment of wound healing. We examined our hypotheses by creating 4 mm punch biopsies on the lower thigh of the left leg (LL) of 12 wk old BERK and hemizygous BERK (hBERK) mice expressing sickle hemoglobin (HbS). Since BERK did not survive wounding, hBERK and control HbA-BERK, expressing normal human HbA and wild type C57/BL6, were used. hBERK appeared to be a suitable model for this study because we found that >5 mo old BERK and hBERK show deep tissue and cutaneous hyperalgesia similar to pain in human SCD (Blood 116:456-65, 2010). Wounds were treated with topical morphine (3 mg/g Eucerin base cream) or with Eucerin base cream twice a day. Behavioral measures of hyperalgesia were assessed by paw withdrawal frequency (PWF) per 10 applications of a 1.0 g von Frey monofilament and paw withdrawal latency (PWL) in response to a radiant heat stimulus, applied to the plantar surface of the hind paw of wounded left leg and unwounded right leg. No hyperalgesia was observed in 12 wk old hBERK (baseline), but wounding led to a sharp increase in PWF and decrease in PWL, in LL for 24h, suggestive of wound-induced pain. Topical application of morphine significantly decreased mechanical and thermal hyperalgesia after 1h and 24h as compared to the base cream treated wound (p<0.05) in LL. Pain measures returned to baseline 2 wks post-wounding in morphine- but base cream-treated hBERK mice exhibited hyperalgesia for 4 wks (last measurement). The right leg exhibited increased mechanical but not thermal hyperalgesia following the wounding of left leg in hBERK. HbA-BERK and C57/BL6 wounds treated with base cream showed an increase in pain which was significantly less than hBERK and reached baseline 2 wks post-wounding, suggesting that wounding results in chronic pain in hBERK mice. Increased pain was accompanied by decreased wound closure in hBERK mice. Morphine-treated mice showed 100% wound closure at 4d Vs 8d in base cream treated hBERK, indicative of morphine-induced augmentation of wound healing. Laser scanning confocal microscopy (LSCM) of whole dorsal root ganglion (DRG) showed that, activating transcription factor 3 (ATF3), a marker of neuronal injury, was highly expressed in left lumbar DRG of base cream Vs morphine treated wounded hBERK, 30d post-wounding (p<0.05), suggestive of ongoing wound-induced neuronal injury which is abrogated by topical morphine. LSCM of 100 micron thick sections of healed wounds showed that substance P (SP), a neuropeptide expressed in nociceptive primary afferents, was significantly increased in base cream treated Vs morphine treated healed wounds 30d post wounding (p<0.01). Increased SP was accompanied by reduced and disorganized protein gene product (PGP 9.5)-ir nerve fibers and vasculature in base cream treated wounds. In contrast, morphine treated wounds showed normally organized dense PGP 9.5-ir nerve fibers and blood vessels replete with nerve and vascular plexus similar to their presentation in normal skin. Together, these data show that wounds stimulate chronic pain accompanied by nerve injury, central sensitization and neurogenic inflammation; and that morphine ameliorates this noxious insult and promotes wound healing. In addition, morphine may directly influence wound healing by promoting angiogenesis. It is possible that chronic pain may contribute to impaired wound healing and that non-healing wounds contribute to chronic pain in SCD. We propose that wound-induced nerve signal conduction (ATF3 in DRG) and neurogenic inflammation/sensitization of primary afferents (SP in skin) may contribute to chronic pain and in turn interfere with wound healing. Our observations suggest that neurochemical and behavioral alterations orchestrated by wounding may contribute to the impairment of healing of leg ulcers in SCD. We speculate that topical application of morphine may ameliorate pain and promote healing of painful leg ulcers in SCD. Disclosures:No relevant conflicts of interest to declare.

Healing effect of quince seed mucilage on T-2 toxin-induced dermal toxicity in rabbit

This study was performed to examine the healing effects of creams prepared from quince seed mucilage on dermal toxicity induced by T-2 toxin. Creams were prepared from quince seed mucilage in eucerin base as 5, 10, and 15% (w/w). To induce dermal toxicity, 100 μg T-2 toxin was dissolved in 12 μl methanol and applied on the shaved skin of rabbit for 2 consecutive days. A separate group received only solvent (methanol). On the third day, the prepared creams were applied on the skin lesions twice a day until complete healing was occurred. Healing, was defined by decreased wound margin, treatment erythema and blisters. Time required for healing was 14, 14, 12, 10, and 9 days for groups of no treatment, eucerin, quince seed cream 5%, 10%, and 15%, respectively. Results obtained by this study shows that quince seed mucilage (15%) has more and better healing effects on dermal toxicity caused by T-2 toxin comparing to no treatment or eucerin cream without mucilage.

Effect of Licorice on Wound Healing In Rabbits

Licorice (Glycyrrhiza glabra) is one of the most widely used medicinal plants and is included in numerous traditional and modern medical preparations. In the present study the healing effect of licorice extract was investigated on open skin wounds in rabbits. Adult Newzeland rabbits of both sexes weighing 1.5 - 2.5 kg were used. Hairs of lower back and left flank of animal were shaved. Full-thickness wound (15x15 mm) was made on the shaved area. Hydroalcoholic extract of licorice was prepared by maceration method. Creams of 5%, 10% and 15% (w/w) extract in eucerin base were prepared and applied 2 times daily. Dexpanthenol ointment was used as standard control. Healing was determined by reduction in wound area. The results of this study proved that licorice cream of 10% was a potent healing agent even better than dexpanthenol cream.

Effect of Achillea millefolium Extract in Wound Healing of Rabbit

Objective: To evaluate the effect of hydroalcoholic extract of Achillea millefolium (yarrow) on the wound healing in rabbit. Material and methods: Wound healing effect of yarrow extract in eucerin base at concentrations of 2, 5 and 10% w/w were tested topically on full thickness excision wound in rabbits. Such effect was compared with phenytoin cream (1%) as a standard healing agent. Results: Yarrow extract creams significantly reduced the rate of wound healing compared to no-treatment, eucerin. Five percent yarrow extract cream showed better healing profile, and the rate of healing was significantly (p<0.01) better than other groups. Conclusion: Yarrow showed a considerable potential for wound healing possibly by accelerating the collagenation and proliferation phase of wound healing.

Influence of Vehicle on Topical Efficacy of 2-Acetylpyridine Thiosemicarbazone and Related Derivatives on in vivo Type 2 Herpes simplex Virus Infections

2-Acetylpyridine semicarbazone (APSC), 2-acetylpyridine thiosemicarbazone (APTSC) and 2-acetylpyridine-4-methyl-3-thiosemicarbazonoe (APMTSC) were evaluated against type-2 herpes simplex virus (HSV-2)-induced genitalis and encephalitis in mice and guinea pigs. The antiviral activity of these compounds was compared with that of acyclovir. With 1,3-butanediol as a topical treatment vehicle, 1% APSC and APTSC showed significant activity against the genital infection in mice, as evidenced by increased survivors and decreased severity of vaginal lesions. Reduced titers of virus recovered from the lesions were also observed with APTSC treatments. Eight different commercially available vehicles were compared to determine in which topically administered 1% APTSC would be most efficacious against the vaginal disease induced in mice. Significant results were observed with Squibb cream base, Eucerin base, and K-Y jelly; these effects were essentially equivalent to using 1,3-butanediol Unibase, Aquaphor, polyethylene glycol, polyvinyl alcohol and petrolatum were less effective as carrier vehicles. The herpesvirus genital infection in guinea pigs was treated with 1% APMTSC and APTSC comparing Squibb cream, Eucerin and K-Y jelly bases; while neither compound exerted striking effects against this infection in any vehicle, 1% APMTSC in Squibb cream was effective in increasing mean survival time and reducing lesion score and titers of recoverable virus. In this experiment, treatments with 1 and 5% acyclovir in polyethylene glycol base were effective in reducing titers of virus from the vaginal area. Orally administered APTSC (12.5, 25, 50 mg/kg/day given twice daily for 7 days) caused moderate prevention of death of mice infected intraperitoneally with HSV-2; subcutaneous injection of the compound was markedly effective with efficacy comparable to that of acyclovir at 120 mg/kg/day.