Abstract
A quantitative analysis of vitamins A and E in commercial ointments containing 0.044% and 0.8% (w/w) of active pharmaceutical ingredients, respectively, was performed using partial least squares models based on FT Raman spectra. Separate calibration systems were prepared to determine the amount of vitamin A in a petrolatum base ointment and to quantify vitamins A and E in a eucerin base one. Compositions of the laboratory-prepared and commercial samples were controlled through a principal component analysis. Relative standard errors of prediction were calculated to compare the predictive ability of the obtained regression models. For vitamin A determination, these errors were found to be in the 3.8–5.0% and 5.7–5.9% ranges for the calibration and validation data sets, respectively. In the case of vitamin E modeling, these errors amounted to 3.7% and 4.4%. On the basis of elaborated models, vitamins A and E were successfully quantified in two commercial products with recoveries in the 99–104% range. The obtained data indicate that the Raman technique allows for accurate analysis of the composition of semisolid formulations in their native state, including low dose preparations.
Highlights
Vitamins A and E, belonging to the group of a lipid-soluble active compounds, are essential nutrients that humans can only acquire through diet
Vitamin A, 3,7-dimethyl-9(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol, is one of some 50 compounds found in nature called retinoids
Quantify α-tocopherol in oil-water emulsions [17] and to determine its content in vegetaHerein, we present the results of simultaneous vitamin A and E quantification in ble oils [18]
Summary
Vitamins A and E, belonging to the group of a lipid-soluble active compounds, are essential nutrients that humans can only acquire through diet. Vitamin E can be present in a variety of forms, including the most common, tocopherol (Figure 1) It exhibits antioxidant properties by scavenging free radicals that can attack DNA or may cause oxidation of polyunsaturated fatty acids [2]. In the case of vitamin E, the α-, β-, γ- and δ- forms of tocopherol, known as E306–E309 additives, are approved for use in the food industry. They can be found in creams and ointments intended to protect skin against aging and the harmful effects of external factors
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
