Ethylenediaminetetraacetic Acid Research Articles

Effects of using different dentin conditioners on dentin regeneration.

This experiment aimed to evaluate the impact of several dentine etching and conditioning agents on growth factors (GFs) liberation from dentine slices. Eighteen dentine slices were obtained from nine premolars divided in to six groups, the slices immersed in one mL test solutions for 5 min; Group 1: white Mineral trioxide aggregate (MTA), Group 2: Phosphate buffered saline (PBS), Group 3: 37% phosphoric acid, Group 4: 17% Ethylenediaminetetraacetic Acid (EDTA), Group 5: 10% Maleic acid (MAc), and Group 6: 0.7% Fumaric acid. The solutions were removed and stored directly at for further detection and quantification of transforming GF beta 1 (TGF-b1), bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor (VEGF) by enzyme-linked immunosorbent assay (ELISA). One-way ANOVA was used to compare the mean release and standard deviation between groups (α = 0.05). Tukey's post hoc applied for multiple comparisons. After five min conditioning of dentine slices, white MTA released the highest level of TGF-b1, BMP2 and VEGF among all groups, followed by 0.7% Fumaric acid with no significant difference between them, but compared to 37% phosphoric acid and PBS groups significant difference observed, which they released the least amount of GFs amongst all groups. Based on the results of this research the detectable release of TGF-b1, BMP2 and VEGF by 0.7% fumaric acid was comparable with white MTA from dentin slices.

A-314 Influence of EDTA on TSH and 17-OHP in Newborn Screening: A Case Study

Abstract Background A 9-month-old infant presented with developmental motor delay. Comprehensive evaluation including microarray and brain MRI revealed no specific abnormalities except for hypotonicity. The patient's perinatal history, including newborn screening test (NST), showed no remarkable findings. Additionally, the infant exhibited facial dysmorphism and microcephaly, leading to another NST to explore potential metabolic diseases. Upon NST, Thyroid-Stimulating Hormone (TSH) levels were 1.86 and 1.27 mlU/L (duplicate testing results; reference value: <12 mlU/L), 17-Hydroxyprogesterone (17-OHP) levels were 0.55 and 0.48 ng/mL (duplicate testing results; reference value adjusted for body weight: <5.0 ng/mL), and total Galactose (Gal) and Galactose-1-Phosphate (Gal-1-P) levels were 1.68 mg/dL (reference value: <8 mg/dL). Methods Upon inspection of the dried blood spot (DBS) sample, the DBS used for the tests partially appeared to be inadequately collected on the absorbent filter paper, which could cause errors related to the disc and specimen itself. Consequently, re-sampling and re-testing were necessary to ensure accuracy. A second NST was performed with the new DBS samples. In the second NST results, most parameters were within the allowable ranges. However, the TSH levels were notably below the detectable limit (<0.7 mlU/L), and the 17-OHP levels were significantly elevated at 332ng/mL, diverging markedly from the initial NST results. To ensure reliability of the measured test results, TSH levels were analyzed using both the existing PerkinElmer equipment and compared with results from a Cobas 8000 system (Roche) and mass spectrometry. Similarly, 17-OHP levels were also compared using mass spectrometry. Results Upon comparing the TSH results, both analytical methods (PerkinElmer and Roche) yielded identical outcomes. However, significant discrepancy was observed when compared to mass spectrometry results. Review of the relevant testing batches showed no significant quality control issues with the NST, leading to clinical suspicion of ethylene-diamine-tetraacetic acid (EDTA)-induced interference. According to existing literature, when NST are conducted using the lanthanide fluorescence method, distortions can occur in DBS containing EDTA, depending on the concentration of EDTA present. In such cases, the EDTA-induced interference may result in false-negative TSH and false-positive 17-OHP results. These discrepancies can be differentiated using standard MS/MS testing. With regards to our case, upon enquiry, the second DBS sample was revealed to be collected from an existing EDTA sample rather than directly from the patient. Thus, adequate DBS discs from the initial sample were obtained and re-analyzed for NST using mass spectrometry, with final results reporting no specific abnormalities. Conclusions The discrepancies observed in TSH and 17-OHP levels between the first and second set of DBS samples were found to be unrelated to other laboratory factors, such as quality control issues. Instead, they were conclusively identified as false-negative and false-positive results associated with interference due to the use of EDTA-containing collection bottles. Mass spectrometry analysis was able to confirm the interference. Clinical awareness should be promoted in order to prevent EDTA-induced interference in NST.

Polyethylene glycol enhanced antibacterial activity of EDTA and ethyl maltol blended PLA against Staphylococcus aureus for biodegradable active packaging

Polylactic acid (PLA) has limited promise as a bio-based antimicrobial packaging material due to its lack of plasticization, which limits antimicrobial agent release and antimicrobial efficiency. PLA were plasticized with polyethylene glycol (PEG) and blended with ethyl maltol or ethylenediaminetetraacetic acid (EDTA) as antibacterial agents by melt extrusion. The effects of combining PEG, EDTA, and ethyl maltol on sheet properties and antibacterial activity were investigated. Incorporation of ethyl maltol created a dense and smooth homogeneous surface, while EDTA addition caused large pores in the PLA sheet microstructure impacting swelling degree, water solubility, and surface hydrophilicity. Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) showed that both ethyl maltol and EDTA addition modified the amorphous and crystalline phases of plasticized PLA. FTIR results suggested interaction between the functional groups of PLA chains, PEG, ethyl maltol or EDTA which affected the glass transition temperature (Tg), degree of crystallinity, and thermal stability of PLA. Adding PEG into PLA-EDTA enhanced antibacterial activity against Gram-positive Staphylococcus aureus (reduction of 1.35 log CFU/mL from the initial inoculum). The higher water solubility of sheets corresponded to a suitable release rate and sufficient amounts of antimicrobial compounds against Staphylococcus aureus. In vitro antifungal testing, PLA-PEG-EDTA sheets did not exhibit a clear zone of inhibition but delayed Penicillium sp. spore formation until day 5 of incubation. PEG plasticized PLA sheets functionalized with ethyl maltol and EDTA showed potential as active biomaterials against food-borne pathogen bacteria Staphylococcus aureus.

Supercritical carbon dioxide chelation extraction of heavy metal ions from drilling fluid waste: Experiment and simulation

This study evaluates the application of supercritical carbon dioxide chelation extraction technology for treating heavy metal ions (Zn2+ and Cr3+) in drilling fluid waste. Through a combination of experimental and molecular dynamics simulation methods, the influence of extraction parameters (temperature, pressure, duration, and chelating agents) on the extraction efficiency are investigated. Findings show that increased duration and pressure significantly improve extraction efficiency, while temperature has a complex effect, initially increasing efficiency but plateauing and slightly decreasing at higher temperatures. The optimum extraction condition with pressure of 220 bar, temperature 348.15 K and an extraction duration of 70 min using ethylene diamine tetraacetic acid (EDTA) as the chelating agent has been determined. Importantly, molecular simulation analysis revealed that citric acid outperforms EDTA in terms of Zn2+ and Cr3+ aggregation by forming larger aggregates with greater numbers of molecules while reducing overall aggregate count. Furthermore, optimization of extraction pressure in the EDTA system shows potential benefits. These results suggest that supercritical carbon dioxide chelation extraction technology has strong potential for environmentally friendly and reliable waste management in the drilling industry. This study represents a significant step forward in developing sustainable solutions for heavy metal removal from drilling fluid waste.

The influence of Fe2+ on the self-assembly of a bipyridine containing homopolymer: From bowl-shaped nanoparticles to vesicles

Metal ion coordination has critical influence on the self-assembly behavior of amphiphilic polymers and the morphology of the obtained assemblies. Herein, an amphiphilic homopolymer with 2,2′-bipyridine (BPy) as side chain is synthesized (noted as PBPyAA), which can self-assemble into bowl-shaped nanoparticles (BNPs) in tetrahydrofuran (THF)/water. Taking advantage of the coordination interaction between BPy and metal ions, Fe2+ is chosen to regulate the self-assembly behavior and the morphology of the assemblies of PBPyAA in two pathways. (Ⅰ) The aqueous solution of Fe2+ with various concentrations is added to the THF solution of PBPyAA during self-assembly. (Ⅱ) Fe2+ is added into the THF solution of PBPyAA before self-assembly, followed by the addition of deionized water to promote the self-assembly. The results show that the pathway Ⅰ facilitates the coordination of BPy and Fe2+. With the increase of the concentration of Fe2+ aqueous solution, the coordination efficiency of BPy increases from 0.419 % to 7.789 %, leading to the transformation of BNPs to vesicles. Though the coordination efficiency of BPy also increases with the concentration of Fe2+ in pathway Ⅱ, which is still quite low of 0.274 % to 0.366 %, and the morphology of the BNPs barely changes. In addition, ethylene diamine tetraacetic acid (EDTA), a strong chelating agent, is also added to promote the competitive complexation with BPy, resulting in the dissociation of BPy and Fe2+ and the reversible transformation from vesicles to BNPs. Overall, the effect of Fe2+ coordination on the self-assembly behavior of PBPyAA in two pathways is investigated and the reversible transformation of BNPs to vesicles is also achieved.

Micellar Nanogels from Alginate-Based Diblock Copolysaccharides.

Alginates aremarine polysaccharides known for their ability to selectively bind calcium ions and form hydrogels. Theyare widely used in biomedical applications but are challenging to produce as nanogels. Here we introduce a self-assembly route to create stable alginate-based nanogels under near-equilibrium conditions. Guluronate (G) blocks, which interact with divalent cations such as Ca2+, Ba2+, and Sr2+, were extracted from alginates and covalently linked through their reducing end to the reducing end of dextran (Dex) chains, forming linear block copolymers that self-assemble into micellar nanogels with a core-corona structure in the presence of these ions. Real-time dynamic light scattering (DLS) and small-angle neutron scattering (SANS) were used to study the self-assembly mechanism of the copolymer during dialysis against divalent ions. For the G12-b-Dex51 copolymer, we achieved spherical micelles with an 8 nm radius and an aggregation number of around 20. Although the type of divalent cation affected micelle stability, it did not influence their size. Micellar nanogels are dynamic structures, capable of ion exchange, and can disassemble with chelating agents like ethylenediamine tetraacetic acid (EDTA).

Fixed-bed natural gas CO2 adsorption performance of ZSM-5 zeolites impregnated with amines

This research studies the fixed-bed natural gas carbon dioxide (CO2) adsorption performance of ZSM-5 zeolites impregnation with different amines: monoethanolamine (MEA), ethylenediamine (ED), ethylenediaminetetraacetic acid (EDTA), and 4-aminophenol. The structural, morphological, and performance characteristics of the modified zeolites were examined through the use of several analytical techniques. These techniques featured Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The thermogravimetric analysis revealed weight losses in two stages linked to the removal of bound water molecules and the breakdown of the hydroxyl structure. Through fixed bed adsorption experiments, it was discovered that ZSM-5 zeolites functionalized with MEA/ED showed the CO2 adsorption capacity. This study investigated how well four different mathematical models—Clark, Yoon-Nelson, Thomas, and Adams-Bohart—could predict CO2 adsorption breakthrough curves from natural gas using non-linear regression methods. The results showed that all models had very high adjusted determination coefficients (Adj. R²) of over 0.99, indicating they matched the experimental data really well for various adsorbents. The analysis highlighted the models' effectiveness in reflecting adsorption kinetics and capacities, particularly noting the superior performance of the Thomas, Adams-Bohart, and Yoon-Nelson models in capturing uniform breakthrough behaviors. These findings underscore the models' utility in enhancing CO2 removal processes in natural gas purification, which is pivotal for optimizing industrial gas processing systems. These results highlight the efficiency of amine-functionalized ZSM 5 zeolites in capturing CO2 from natural gas streams.

EDTA-Assisted MPT-MS for Trace Analysis of Heavy Metals in Fireworks.

A novel method was developed for the rapid detection of heavy metals in firework solutions with high sensitivity and minimal pretreatment by enriching them with ethylenediaminetetraacetic acid (EDTA) reagent and analyzing them using microwave plasma torch mass spectrometry (MPT-MS). Quantitative results showed that the limits of detection and quantification for heavy metals (Pb, Ba, Sr, and Ag) ranged from 0.05 to 0.25 and 0.38 to 0.71 μg·L-1, respectively. The linear dynamic ranges covered at least two orders of magnitude, with correlation coefficients exceeding 0.99. Fireworks from five regions in China were also analyzed quantitatively, detecting heavy metals including Pb, Ba, Sr, and Ag, with recovery rates ranging from 87.9% to 107.5%. Good separation between the firework samples from different regions was achieved by using element ratios and principal component analysis (PCA). These results from the preliminary study showed that the EDTA-assisted MPT-MS combined with PCA is a powerful tool for characterizing firework samples and tracing them back to their sources, which is valuable to effectively regulate and manage banned fireworks.

Comparative analysis and mechanistic insights into polysorbate 80 stability differences in biopharmaceutical buffer systems

Polysorbate 80 (PS80) is a non-ionic surfactant extensively utilized in biopharmaceutical formulations for stabilizing proteins. However, PS80 degradation has become a widespread concern throughout the industry over the past decade. In this work, the impact of most frequently employed pH/buffer systems on the stability of PS80 was assessed. PS80 degraded fastest in histidine buffer, followed by acetate and succinate buffers, whereas it remained stable in citrate, phosphate and tris buffers. When there was PS80 degradation, the extent of degradation was found to be pH-dependent. The predominant degradation pathway was oxidation mainly triggered by metal ions. The varying stability of PS80 across different pH/buffer systems was attributed to the role of buffer agents, which can either promote or inhibit the oxidation process through their interactions with metal ions. Specifically, buffers except histidine exhibited metal ion chelation similar to ethylenediaminetetraacetic acid (EDTA), which can suppress the oxidation of PS80, although the effectiveness of chelation varies to different extents. Furthermore, the binding capacity appeared stronger at higher pH in acetate and succinate buffers. Conversely, histidine was reported to form pro-oxidant complexes with metal ions to accelerate PS80 degradation, especially at higher pH levels. Our work for the first time offers a comprehensive understanding of PS80 oxidation in biopharmaceutical buffer systems. This provides a strong foundation for buffer and excipient selection in parenteral formulations.

Comparative evaluation of wettability of AH plus, Ceraseal and Guttaflow bioseal root canal sealers on root canal dentin: An in vitro study

Abstract Context: Effective root canal treatment relies on the ability of sealers to wet and penetrate the root canal system, ensuring a tight seal and preventing bacterial leakage. The current study investigates the wetting ability of newer root canal sealers by evaluating their contact angle. Aim: The objective of the present study was to evaluate and compare the wetting behavior of Guttaflow bioseal, Ceraseal, and AH plus root canal sealers. Materials and Methods: Decoronation and apical third resections of thirty extracted singlerooted human mandibular premolars were done. The roots were then split longitudinally into two halves buccolingually. The root dentin surfaces were treated using 10 mL 3% sodium hypochlorite for 5 min, 10 mL of 17% ethylenediaminetetraacetic acid for 5 min, and 2% chlorhexidine for 10 min. Then, these sixty dentin bars were randomly divided into three groups (n = 20): Group 1 – AH Plus, Group 2 – Ceraseal, and Group 3 – Guttaflow bioseal. The controlled volume of 0.1 mL sealer has been dispensed onto the dentin specimen using a micropipette. The contact angle of a drop of sealer was measured after 5 min using Ossila goniometer. Statistical Analysis Used: The data were analyzed using one-way analysis of variance and Tukey post hoc tests. The statistical significance was at P < 0.05. Results: The mean contact angle values for AH Plus sealer were lower when compared to the other two sealer groups which is statistically highly significant. Conclusions: AH Plus showed lesser contact angle thereby better wettability than Ceraseal and Guttaflow bioseal on the root canal dentin surface.

Enhancing Selective NH4+ Recovery from Wastewater Using Modified Zeolite-Based Flow Electrode Capacitive Deionization

Despite flow-electrode capacitive deionization (FCDI) is an emerging technology for desalination, contaminant removal, and resource recovery, the application of conventional FCDI in wastewater treatment is hindered by the electrode selectivity and material costs. In this study, we synthesized a low-cost ammonium (NH4+) adsorption electrode material by modifying zeolite using ethylenediaminetetraacetic acid disodium salt (EDTA-2Na). The flow electrode prepared by the mixture of EDTA-zeolite and carbon black exhibits a high selectivity and adsorption capacity for the recovery of NH4+ from wastewater. The NH4+ in wastewater passes through the ion exchange membrane and is rapidly adsorbed by the modified zeolite through ion exchange, while Na+ is retained in the electrolyte. The decrease in NH4+ concentration and the increase in Na+ concentration in the catholyte lead to a significant change in ion concentration gradient across the membrane. Consequently, the transmembrane selectivity between NH4+ and Na+ reached 3.46. We validated the feasibility of NH4+ recovery using FCDI with food waste fermentation supernatant. Under optimal operating conditions, 99.15% of the NH4+ in the fermentation supernatant was removed, and 95.92% of the NH4+ in the electrolyte was stored in the EDTA-zeolite. By gravitational settling, the NH4+-rich modified zeolite was separated from carbon black and could be utilized as nitrogen fertilizer. Meanwhile, the mixture of carbon black and brine was used to prepare a fresh electrode suspension. In brief, the FCDI system exhibits a satisfying NH4+ recovery performance and demonstrates a sustainable wastewater resource recovery strategy.

The Effect of Electrolyte pH and Impurities on the Stability of Electrolytic Bicarbonate Conversion.

Electrolytic bicarbonate conversion holds the promise to integrate carbon capture directly with electrochemical conversion. Most research has focused on improving the faradaic efficiencies of the system, however, the stability of the system has not been thoroughly addressed. Here, we find that the bulk electrolyte pH has a large effect on the selectivity, where a higher pH results in a lower selectivity. However, the bulk electrolyte pH has no effect on the stability of the system. A decrease in CO selectivity of 30% was observed within the first three hours of operation in an optimized system with 3 M KHCO3 and gap between the membrane and electrode. Single-pass electrolyte experiments at various constant pH values (8.5, 9.0, 9.5, and 10.0), show that only at a pH of 10 the CO selectivity was stable during three hours, reaching a faradaic efficiency toward CO of only 18% as compared to an initial 55% at pH 8.5. Trace metal impurities present in the electrolyte were found to be the cause of the decrease in stability as these deposit on the electrode surface. By complexing the trace metal ions with ethylenediaminetetraacetic acid (EDTA), the metal deposition was avoided and a stable CO selectivity was obtained.

Rice husk-derived nitrogen-doped graphitic carbon/graphene nanosheets self-assembly for high-performance supercapacitors with chemical blowing method

Carbon materials derived from waste biomass are increasingly popular in energy devices due to their renewability, environmental benefits, and rich heteroatom functionalization. Herein, a simple and low-cost method for synthesising rice husk-derived nitrogen-doped graphitic carbon/graphene nanosheets (RNGNs) by chemical blowing was developed. Carbon was derived from rice husk (RH), while nitrogen was sourced from ethylenediaminetetraacetic acid and iron sodium salt hydrate (EDTA-FE), serving multiple roles as a blowing agent, an activating agent and a graphitization catalyst in a closed system. This approach facilitates the formation of a unique 3D structure of 2D graphene self-assembly on the wrinkled surface of graphitic carbon. As an electrode material for supercapacitors, the obtained RNGN800–1:1 with high nitrogen content (5.02 %) shows good electrical conductivity and a large specific surface area (1130 m2 g−1). These characteristics bring about an impressive electrochemical performance (334.5 F g−1 at 0.5 A g−1, 75.6 % retention at 20 A g−1). Moreover, when assembled into symmetrical device, the RNGN800–1:1 demonstrates a high power density of 9.4 kW kg−1and an excellent energy density of 15.2 Wh kg−1 with outstanding long-term cyclability (remains 94.77 % after 25000 cycles). This study introduces a straightforward and environmentally friendly approach for synthesizing nitrogen-doped graphitic carbon/graphene nanosheets from sustainable biomass resources. The resulting material is expected to have broad applications across various areas, including adsorption, catalysis, and energy storage.

Remediation of fluoride-contaminated wastes: Chelator-assisted washing and subsequent immobilization using CaO and H3PO4

Fluoride (F) contamination in industrial waste is a significant challenge for sustainable materials recycling. Existing techniques for mitigating F contamination focus on immobilization, converting F compounds to insoluble forms while leaving the total F content untreated. Chelator-assisted washing is considered a promising alternative remediation strategy that can indirectly release F by entrapping and dissolving F-bearing minerals. This study evaluates the effectiveness of chelator-assisted washing in removing F from three real F-contaminated waste samples (TCS-49, TCS-51, and TCS-52) by treating with four different chelators, ethylenediaminetetraacetic acid (EDTA), ethylenediamine N,N′-disuccinic acid (EDDS), diethylenetriaminepentaacetic acid (DTPA), and 3-hydroxy-2,2′-imino disuccinic acid (HIDS). The influence of key washing variables, including chelator type, solution pH, chelator concentration, washing time, and liquid-to-solid (L/S) ratio toward F extraction was assessed and optimized for attaining the maximum extraction. All chelators exhibited the highest F extraction from TCS-49 and TCS-51 at pH 11, whereas in TCS-52 it showed a discrete extraction pattern. Under optimized conditions (concentration, 10 mmol L−1; pH, 11; washing duration, 3 h; and L/S ratio, 10:1), EDTA outperformed the other chelators, enhancing F extraction by 2.1 and 1.2 times for TCS-49 and TCS-51, respectively, compared with those of control treatments. However, for TCS-52, the efficiency of EDTA was analogous to that of the control under the same washing conditions. The linear correlation between the extracted F and F-containing minerals suggests that the chelator-induced F removal from contaminated waste involves the entrapment and dissolution of F-bearing minerals, especially Ca and Fe. The subsequent post-washing immobilization of chelator-washed waste residues using CaO or H3PO4 significantly reduced the content of leachable F under the regulatory limit.

Effect of 532nm and 589nm Diode-Pumped Solid-State (DPSS) Lasers with Different Doses on Concentration of Total Protein and Albumin in Vitro

Objective: To test the effects of Low-level laser irradiation at 532nm and 589nm with different doses and exposure times on total protein and albumin in blood plasma. Method:This experimental study was conducted from November 2021 to April 2022 at Mustansiriyah University, Baghdad, Iraq, and comprised blood samples from healthy adults. The samples were stored in tubes containing anticoagulant ethylenediaminetetraacetic acid. Photoradiation was managed using green laser 532nm and yellow laser 589nm diode-pumped solid-state lasers with different doses. For total protein test and albumin test, each sample was divided into 3 equal aliquots. The first was control without radiation, the second was irradiated by a laser at 532nm, and the third was irradiated at 589nm with 30J/cm², 50J/cm², 70J/cm² and 90 J/cm² doses of irradiation. All samples were examined by using a spectrophotometer device. Data was analysed using SPSS 25. Results: There were 24 samples from healthy adults in all.The most significant difference in total protein concentration occurred at dose 70J/cm² for both wavelengths compared to the controls (p<0.05). When this optimal dose was applied to the albumin test, a clear decrease in the albumin concentration was found for both 532nm and 589nm lasers (p<0.05). Conclusion: At wavelengths of 532nm and 589nm, the laser light induced processes that caused a reduction in total protein and albumin. Key Words: Edetic Acid, Lasers, Plasma, Light, Anticoagulants, Albumins

Impact of different disinfection protocols on the bond strength of NeoMTA 2 bioceramic sealer used as a root canal apical plug (in vitro study)

IntroductionTreatment of an immature permanent tooth required a special disinfection protocol due to the presence of thin radicular walls, which are prone to fracture. Mineral Trioxide Aggregate (MTA) has been proposed as a root repair material for root canal treatment. The aim of this in vitro study was to compare the push-out bond strength of conventional White MTA cements and second generation NeoMTA 2 in imitated immature roots treated with different disinfection protocols, which are 5.25% sodium hypochlorite (NaOCl), followed by 17% ethylenediaminetetraacetic acid (EDTA), and NaOCl, followed by 20% etidronic acid (HEBP).MethodsThe root canals of freshly extracted single-root teeth were manually prepared until 90 K-file to imitate immature roots. Roots were randomly divided into four groups (G) according to the disinfection protocol (n = 15 per group). where G1 (NaOCl + EDTA + White MTA) and G2 (NaOCl + EDTA + NeoMTA 2) While G3 (NaOCl + HEBP + White MTA) and G4 (NaOCl + HEBP + NeoMTA 2) All groups were activated with manual agitation. All specimens were incubated for 48 h. The apical third of each root was perpendicularly sectioned to attain a slice of 3 mm thickness. Push-out bond strength values were assessed using a two-way ANOVA and a Student’s t test.ResultsG3 and G4 that were treated with HEPB showed higher significant push-out bond strength mean values than G1 and G2 treated with an EDTA chelating agent. Irrespective of the chelating agent used, it was found that both NeoMTA 2 and White MTA had no significant influence on push-out bond strength mean values (p ≤ 0.05).ConclusionThe combined use of 5.25% NaOCl and 20% HEBP increased the push-out strength values of both NeoMTA 2 and White MTA, rendering them suitable to be used as an alternative chelating agent to EDTA.

The impact of calcium sequestration on the material and structural properties of acid milk gels and corresponding cream cheeses

SummaryWe report on the role of ethylenediaminetetraacetic acid (EDTA) in addition to the structural and material properties of acid milk gels formed via addition of glucono‐delta‐lactone (GDL) and these properties of corresponding analogue cream cheeses prepared from these gels. Increasing addition of EDTA to cheese milks prior to acidulation reduced the concentration of insoluble calcium in the milks. Acid gelation of milks, as measured by small deformation rheology, showed a decreased rate of gelation and lower relative elastic modulus of gels with increasing EDTA concentration. The sequestration of colloidal calcium phosphate from the casein micelle by the addition of EDTA led to the lower gelation rate and elastic modulus. The lower extent of ionic binding is considered the causative mechanism for formation of weaker gel structures. Cream cheeses prepared using acid gels with GDL acidulant were compositionally unaffected by EDTA concentration. However, variations in G′ observed in the acid gels containing increasing levels of EDTA correlated with large deformation material property changes in cream cheese analogues. In addition, the increasing inclusion of EDTA in cheese milks translated to reduced water‐holding capacity of the cream cheeses prepared from those milks. These results provide insights on the specific role of milk calcium on the structure, material and physical properties of acid milk gels and corresponding cream cheese analogues.

Controllable heat release of supercooled Erythritol-based phase change materials for long-term thermal energy storage

Transeasonal heat storage in organic phase change materials (PCMs) present a promising solution to the intermittent nature of renewable energy. However, PCMs are prone to spontaneous crystallization during storage, leading to the loss of stored latent heat in low-temperature environments. In this study, we incorporated tetrasodium ethylenediaminetetraacetic acid (EDTA-4Na) and superabsorbent polymer (SAP) to erythritol (ERY), referred to as EES-PCMs, to overcome these challenges and achieve more controllable and stable thermal energy storage. The incorporation of EDTA-4Na and SAP into ERY significantly improves the supercooling stability and phase change enthalpy. The optimal ratio (EES-PCMs-2) of phase change enthalpy reaches an impressive 286.62 J/g, with stable performance maintained for 120 days at room temperature. The EES-PCMs-2 exhibits exceptional thermal cycling stability, retaining its properties even after 100 cycles. A novel air-triggered crystallization method is demonstrated, enabling a temperature increase from room temperature to 48.21 °C in 320 s after being exposed to air for long-term storage. This innovative approach effectively overcomes the limitations of traditional triggering mechanisms, providing a straightforward and efficient method for thermal management. The high thermal storage capacity, stability, and controlled exothermic properties of EES-PCMs position them as promising candidates for applications in seasonal solar thermal energy storage.

The impact of varying EDTA dosage on the flower-like morphology and thermoelectric properties of Ce-doped Bi2Te₃

Thermoelectric materials have significant applications in energy utilization and environmental protection. The effect of different EDTA (ethylenediaminetetraacetic acid) dosages on the flower-like morphology and thermoelectric properties of Ce-doped Bi2Te3 nanoparticles were investigated. The Ce-doped Bi2Te3 nanoparticles were sucessfully prepared via the hydrothermal method, and the influence of EDTA dosage on the morphology, structure, and thermoelectric properties of the materials was analyzed. The experimental results showed that an appropriate amount of EDTA can promote the formation of a flower-like morphology in Ce-doped Bi2Te3 nanomaterials and enhance their thermoelectric properties. The ZT values of the x = 0.15 sample made by the better flower-like morphology of nanopowders are all around 1, which reach 1.15 at 398 K. This work demonstrates the synergistic effects of combining nanostructure engineering and chemical doping strategies for thermoelectric performance enhancement.

Effects of anticoagulants time storage on stable isotope values of crocodilians’ blood tissues

ABSTRACT Rapid coagulation of reptile blood often hinders its use in studies in remote and difficult-to-access areas, necessitating chemical preservation. Therefore, understanding the potential effects of anticoagulants on the isotopic compositions of blood is essential to avoid issues in interpreting the results for ecological studies. In this study we aimed to verify whether the storage time of the blood tissue in anticoagulants can influence its isotopic compositions of the broad-snouted caiman (Caiman latirostris), an ectothermic top predator from eastern South America. Blood samples were obtained from ten adult females of C. latirostris from a commercial breeding facility in 2015. Samples were stored in vials containing ethylenediaminetetraacetic acid (EDTA) and sodium heparin (SH) and centrifuged after 2 and 8 h to separate red blood cells and plasma. No effect of time was found on the δ13C and δ15N of whole blood, plasma, and red blood cells in contact with the two types of anticoagulants, EDTA and SH. The findings have practical implications for researchers in this field, as they suggest that anticoagulants can be used effectively for at least eight hours under refrigeration.