The present research is focused on the preparation of a variety of solvent extracts using different parts of Rhus chinensis Mill. for the estimation of phytochemicals and to perform biological activities. The highest phenolic and flavonoid contents were recorded as 141.48 ± 0.67 mg GAE/g in methanol root extract and 54.34 ± 0.28 mg QE/g in ethyl acetate root extract. Ethyl acetate root extract displayed an IC50 of 7.83 ± 0.18 µg/mL in the DPPH assay. TPC and TFC were found moderately correlated with antioxidant activity. The root and leaf extracts showed antibacterial activities comparable to those of standard drugs against the gram-positive and gram-negative bacteria. The MIC and MBC for root extract against Staphylococcus aureus were 7.8125 mg/mL and 15.625 mg/mL respectively. Similarly, the MIC and MBC for leaf extract against Escherichia coli were 15.625 mg/mL and 31.25 mg/mL respectively.