GC-MS Analysis and Antioxidant Evaluation of Ativisa Root Extract

Abstract

Plants provide an unlimited source of bioactive metabolites that have been utilized in the pharmaceutical industry as a source of diet and medication. Free radicals are known to be the major aspect of oxidative stress that creates damage to the cell. The natural sources of antioxidants which preventing and repairing the damage caused by the free radicals to the cells. Aconitum heterophyllum (Ativisa) belongs to family Ranunculaceae is traditionally utilized externally as well as internally for treating various human diseases such as rheumatic fever, painful joints, gastroenteritis, diarrhoea, oedema, Bronchial asthma. In the present study ethanolic and ethyl acetate extract of the roots of Acontium heterophyllum was investigated for its antioxidant activity and phytochemical composition using GC-MS. GC-MS studies exhibited the existence of 24 phytochemical compounds in ethanol root extract and 31 phytochemical compounds in ethyl acetate root extract. The major phytochemicals detected in ethanolic and ethyl acetate roots extracts of Acontium heterophyllum were Alpha-D-Glucopyranoside beta-D-Fructofuranosyl, Benzophenone semicarbazone, Dodecanoic acid ethyl ester, Ethyl iso-allocholate, l-(+)-Ascorbic acid 2,6 dihexadecanoate, Phytol, Methyl ricinoleate, 13-docosenamide(z), 1,2,4-Triazol-3-amine5-(1,3,5-trimethyl-4-pyrazolyl)amino. The antioxidant potency of ethanol and ethyl acetate root extracts of Acontium heterophyllum on DPPH (23.05% and 21.05 %) and NO radicals (48.58% and 46.70%) at the highest concentration (500 μg/ml) compared to standard ascorbic acid. These findings suggest Acontium heterophyllum could be used as an alternative source for the investigation of new antioxidant agents.