A high-sensitivity planar microfluidic sensor is proposed in this paper based on composite left/right-handed transmission line (CRLH TL). By employing the series zeroth-order resonance (ZOR), the resonance of proposed sensor is only determined by surface capacitance and inductance. The short-circuit structure reduces the energy loss due to edge radiation and generates a highly concentrated electric field on the top surface of the substrate, which can be used to detect the dielectric loading samples. The presented sensor has been simulated, fabricated and tested. The sensor on the printed circuit board (PCB) is integrated with the fluidic container made of polydimethylsiloxane (PDMS) to form the test device. The water-ethanol binary mixture and sucrose solution are used to proof the concept. Finally, the prediction model for the water-ethanol mixture based on measured parameters has been established and the analysis of prediction error has been carried out. The sensor works around 4 GHz, and the maximum sensitivity reaches 1.04. Due to the high sensitivity and simple structure, the proposed structure is a competitive candidate for the applications including bio-sensing and portable biomedical device.