Ethanol-soluble Portion Research Articles

14C-Metabolism During Growth and Shoot Formation in Tobacco Callus Cultures

Summary Tobacco callus grown under shoot-forming and non-shoot-forming conditions was incubated in 14C-glucose, 14C-acetate or 14C-bicarbonate on different days in culture. 14CO2 production, and 14C incorporation into ethanol-soluble and ethanol-insoluble fractions was greater in shoot-forming than non-shoot-forming tissues. Greatest radioactivity from all substrates was in the ethanol-soluble portion, which was further fractionated into lipids, amino acids, sugars and organic acids. Greater conversion of 14C-glucose and 14C-acetate into these various fractions took place in shoot-forming than in the growing tissues, while the reverse was observed with 14C-bicarbonate. The differences in the metabolic patterns between shoot-forming and non-shoot-forming tobacco callus were in concert with the developmental behavior of the tissues.

14C-metabolism during callus induction in tobacco

Tobacco pith-phloem explants and callus were incubated in 14C-glucose, 14C-acetate or 14C-bicarbonate on different days in culture in the dark. 14CO2 production and 14C incorporation into ethanol-insoluble components were generally greater in the subcultured callus than in the pith-phloem explants during days 0 to 5 in culture. Greatest radioactivity from all substrates was in the ethanol-soluble portion, which was further fractionated into lipids, amino acids, sugars and organic acids. Although incorporation into the different fractions varied with the substrate, the patterns of labelling were relatively similar in the two tissues. The greater wound metabolism in the subcultured callus in comparison to the pith-phloem explant during the induction phase of callus formation was correlated with the earlier visible initiation of cell proliferation in the subcultured tissue.

Studies on Quality Assurance of Cosmetics. VI. Detection of a Micro Amounts of Coal-tar Dyes in Cosmetic containing Anion Surface Active Agents

An ion exchange method is described for the separatory detection of a microquantity of coal-tar dyes in cosmetics which contain a large quantity of anionic surfactant. In this method, a sample (3-5g) is warmed on a water bath to remove volatile components and then dissolved in 20 ml of ethanol. The ethanol-soluble portion is passed through a column of 10 ml of DEAE-Sephadex A-25, and then the column is washed with 100 ml of 30% ethanol. The dyes are eluted with hydrochloric acid-30% ethanol mixture (1 : 9), at a flow rate of 0.4 ml/min, until they have completely been eluted. The effluent is evaporated on a water bath, the residue is dissolved in 1-2 ml of ethanol, and this ethanolic solution is submitted to thin-layer chromatography for the identification of each dye. This method was found to give a satisfactory result in the detection of a microquantity of coal-tar dyes in various cosmetics, shampoo, and kitchen-use liquid detergents containing a large amount of anionic surfactant.

The carbohydrate of human semen.

SUMMARY The carbohydrates of human semen are partly dialysable and ethanol-soluble, and partly non-dialysable and ethanol-insoluble. The dialysable and ethanol-soluble portion contains fructose as the principal free sugar, and, in addition, inositol, sorbitol, glucose, ribose, fucose, small amounts of certain other sugars and a polysaccharide which, on acid hydrolysis, yields mannose, fucose and amino sugar. The non-dialysable and ethanol-insoluble portion is even richer in polysaccharide material. It contains, in a bound form (g./100 ml. semen): 0·2 amino sugar, 0·1 sialic acid, and 0·4 orcinol-reactive carbohydrate material which on acid hydrolysis yields galactose, mannose, and fucose, but only a trace of glucose. On incubation of human semen at room temperature, a proteolytic breakdown of seminal proteins sets in, accompanied by a release of carbohydrate in an ethanol-soluble form. Glycogen occurs in human semen in traces only.

キンモクセイ花の成分研究 (第1報)

The flowers of Osmanthus fragrans Lour. var. aurantiacus Makino were soaked in petroleum ether immediately after collection, digested for one week, and filtered with pressing. The aqueous layer of the filtrate was extracted with ether. The residual flowers were then digested with warm dehydrated ethanol for 16 hours.1) The portion soluble in petroleum ether is composed of concretes amounting to 0.214% of the original flowers and its treatment with cold dehydrated ethanol separates it into 0.163% of absolutes and 0.043% of flower wax, which is chiefly composed of triacontane, C30H62.2) The ether solution was chromatographically purified and p-hydroxyphenethyl alcohol C8H10O2, was isolated.3) The ethanol-soluble portion yielded D-mannitol.4) The water-soluble portion was fractionated with lead acetate and basic lead acetate. D-Mannitol was isolated from the filtrate and the presence of D-glucose and D-fructose was detected by paper chromatography. The precipitate obtained by lead acetate and the portion soluble in ethanol, yielded succinic acid.