Abstract
Summary Tobacco callus grown under shoot-forming and non-shoot-forming conditions was incubated in 14C-glucose, 14C-acetate or 14C-bicarbonate on different days in culture. 14CO2 production, and 14C incorporation into ethanol-soluble and ethanol-insoluble fractions was greater in shoot-forming than non-shoot-forming tissues. Greatest radioactivity from all substrates was in the ethanol-soluble portion, which was further fractionated into lipids, amino acids, sugars and organic acids. Greater conversion of 14C-glucose and 14C-acetate into these various fractions took place in shoot-forming than in the growing tissues, while the reverse was observed with 14C-bicarbonate. The differences in the metabolic patterns between shoot-forming and non-shoot-forming tobacco callus were in concert with the developmental behavior of the tissues.
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