Established Insect Cell Lines Research Articles

Effect of juvenile hormone on macromolecular synthesis of an insect cell line.

Effect of juvenile hormone activity of juvenile hormone I and several juvenile hormone mimics (JHM) were investigated on cell growth and macromolecular synthesis of an established insect cell line derived from mosquito (Culex molestus) ovary. The cell growth in medium with 10-4 M methoprene (ZR-515) or 10-4 M farnesol was perfectly inhibited. The cell shape changed into slender or fibloblast like shape. These compounds inhibited also DNA, RNA and protein syntheses. RNA synthesis was most effectively inhibited than others. Metho-prene is a stronger inhibitor of RNA and protein syntheses than puromycin or actinomycin D. The cells incubated in medium with methoprene for one day recovered the cell growth 2 weeks after the methoprene treatment and then the cell shape also recovered. RNA synthesis inhibited by methoprene was also recovered 90min after the methoprene treatment. DNA, RNA and protein syntheses in the cells treated with juvenile hormone were immediately inhibited but the inhibition was reversible.

Replication of baculoviruses in established insect cell lines: Phenomenon of attenuation

The experimental data discussed above confirm that many insect cell lines are suitable for Baculoviruses, especially for the production of NPV after primary virus infection. However prolonged undiluted passagein vitro may result in virus variation and a decline of virulence for the original host.

Insect cell nutrition: emphasis on sterols and fatty acids.

The requirements for, or the utilization of, vitamins, sugars, amino acids, and lipids, as determined with established insect cell lines, are reviewed. Also discussed is the importance of inorganic salt balance and osmotic pressure. Nutrient requirements, as determined in experiments with established cells, are compared to media formulations based on the analysis of insect hemolymphs and to the dietary requirements of insects. It is suggested that culture media formulated in strict agreement with hemolymph analysis may be unnecessarily complex and do not ensure maximum growth.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium.

An established insect cell line (AC20) from the leafhopperAgallia constricta has been adapted to a mammalian cell culture medium based on the formulation of two commercially available media. The cell population doubling time of the adapted line in this medium is approximately 45 hr at 30°C.

VI. Comparative lipid analysis of Aedes aegypti and monkey kidney cells (MK-2) cultivated in vitro.

Lipid composition of several types of mammalian cells has been reported, such as that of the mouse “L” cell (Anderson et al., 1969; Bailey and Menter, 1967) and the monkey kidney cell (MK-2) (Makino et al., 1970), but the arthropods (Barroso et al., 1969; Agarwal and Rao, 1969; Fast, 1966) or the established insect cell lines have not been fully analyzed. Arthropods have been reported as having an active lipid metabolism and appear to depend on fatty acids rather than carbohydrates as a main energy source (O’Brien and Wolfe, 1964). However, arthropods do require a source of dietary sterols since they are unable to synthesize the sterol ring from acetate or mevalonate, but are capable of converting sterol to cholesterol (O’Brien and Wolfe, 1964).KeywordsTotal LipidNeutral LipidSterol EsterSpinner FlaskMonkey Kidney CellThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Replication of a nuclear polyhedrosis virus in an established insect cell line

Replication of a nuclear polyhedrosis virus in an established insect cell line

Established Insect Cell Line from the Cabbage Looper, Trichoplusia ni

CULTURED mammalian cells are finding increasing use as a basis for conducting physiological and biochemical investigation outside the organism. Insect cell lines have originated from primary explants of embryonic tissue or tissues from larval and pupal stages of the donor. Cells from primary explants of embryonic tissue from the fruit fly, Drosophila melanogaster; the mosquito, Aedes aegypti; leaf hoppers, Agallia constricta and A. quadripunctata; and cockroaches, Periplaneta americana and Blabera fusca, continued to multiply and were subcultured1–4. Primary cultures of larval tissues from, the silkworm, Bombyx mori; the rice stem borer, Chilo suppressalis; and mosquitoes, Aedes aegypti, A. albopictus and Anopheles stephensi, were subcultured and developed into cell lines5–11. Cells originating from pupal ovaries of the moth, Antheraea eucalypti, have also been established as a cell line12. This article reports the first successful establishment of a cell line derived from adult tissue of the cabbage looper, Trichoplusia ni.

Adaptation of Established Insect Cell Lines to a Different Culture Medium

Adaptation of Established Insect Cell Lines to a Different Culture Medium