Abstract
Effect of juvenile hormone activity of juvenile hormone I and several juvenile hormone mimics (JHM) were investigated on cell growth and macromolecular synthesis of an established insect cell line derived from mosquito (Culex molestus) ovary. The cell growth in medium with 10-4 M methoprene (ZR-515) or 10-4 M farnesol was perfectly inhibited. The cell shape changed into slender or fibloblast like shape. These compounds inhibited also DNA, RNA and protein syntheses. RNA synthesis was most effectively inhibited than others. Metho-prene is a stronger inhibitor of RNA and protein syntheses than puromycin or actinomycin D. The cells incubated in medium with methoprene for one day recovered the cell growth 2 weeks after the methoprene treatment and then the cell shape also recovered. RNA synthesis inhibited by methoprene was also recovered 90min after the methoprene treatment. DNA, RNA and protein syntheses in the cells treated with juvenile hormone were immediately inhibited but the inhibition was reversible.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.