Essential Role Research Articles

“Family doctors are also people”: a qualitative analysis of how family physicians managed competing personal and professional responsibilities during the COVID-19 pandemic

BackgroundFamily physicians (FPs) fill an essential role in public health emergencies yet have frequently been neglected in pandemic response plans. This exclusion harms FPs in their clinical roles and has unintended consequences in the management of concurrent personal responsibilities, many of which were amplified by the pandemic. The objective of our study was to explore the experiences of FPs during the first year of the COVID-19 pandemic to better understand how they managed their competing professional and personal priorities.MethodsWe conducted semi-structured interviews with FPs from four Canadian regions between October 2020 and June 2021. Employing a maximum variation sampling approach, we recruited participants until we achieved saturation. Interviews explored FPs’ personal and professional roles and responsibilities during the pandemic, the facilitators and barriers that they encountered, and any gender-related experiences. Transcribed interviews were thematically analysed.ResultsWe interviewed 68 FPs during the pandemic and identified four overarching themes in participants’ discussion of their personal experiences: personal caregiving responsibilities, COVID-19 risk navigation to protect family members, personal health concerns, and available and desired personal supports for FPs to manage their competing responsibilities. While FPs expressed a variety of ways in which their personal experiences made their professional responsibilities more complicated, rarely did that affect the extent to which they participated in the pandemic response.ConclusionsFor FPs to contribute fully to a pandemic response, they must be factored into pandemic plans. Failure to appreciate their unique role and circumstances often leaves FPs feeling unsupported in both their professional and personal lives. Comprehensive planning in anticipation of future pandemics must consider FPs’ varied responsibilities, health concerns, and necessary precautions. Having adequate personal and practice supports in place will facilitate the essential role of FPs in responding to a pandemic crisis while continuing to support their patients’ primary care needs.

Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division.

Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find proteins containing photosynthetic reaction centre (PRC) barrel domains that play an essential role in archaeal cell division. We rename these proteins cell division protein B 1 (CdpB1) and CdpB2. Depletions and deletions in their respective genes cause severe cell division defects, generating drastically enlarged cells. Fluorescence microscopy of tagged FtsZ1, FtsZ2 and SepF in CdpB1 and CdpB2 mutant strains revealed an unusually disordered divisome that is not organized into a distinct ring-like structure. Biochemical analysis shows that SepF forms a tripartite complex with CdpB1/2 and crystal structures suggest that these two proteins might form filaments, possibly aligning SepF and the FtsZ2 ring during cell division. Overall our results indicate that PRC-domain proteins play essential roles in FtsZ-based cell division in Archaea.

Navigating the New Normal: A Study on the Impacts of the Pandemic on Electronic Resource Management at Academic Libraries

During the COVID-19 pandemic, academic libraries saw a shift toward remote learning leading to increased dependency on electronic resources. This article explores the implications of the pandemic on electronic resource management, focusing on modifications in budget allocation, licensing, accessibility, and staffing. Drawing from a data set of 120 survey responses in 2023 in the United States, this article illustrates the challenges academic libraries faced and changes made during the pandemic. These findings reiterate the essential role of electronic resources and emphasize the need for academic libraries to continually adapt their management strategies to meet user needs in the post-pandemic era.

Coverage of Congenital Anomalies: Ensuring Lasting Smiles Act (ELSA) in Legislation.

The Ensuring Lasting Smiles Act (ELSA) is a federal bill which would require all health federally regulated insurance products to cover the full treatment of a congenital anomaly or birth defect until complete restoration of normal function or appearance. ELSA has been a key federal priority for the Legislative Advocacy Committee of the American Society of Plastic Surgeons (ASPS) since the 115 th Congress in 2018. Although it has gained momentum, particularly in the 117 th Congress, the bill remains stalled. Congressional Budget Office review, conducted in March 2022, appears to have overestimated this bill's cost for the private sector, secondary to an alleged lack of data on the prevalence of the conditions, extent of treatments and their costs, and the current extent of private sector coverage. The present review highlights the progress of the ELSA bill and serves to illustrate the hurdles involved in passing significant healthcare bills. This review emphasizes the need for healthcare professionals to provide their congressional representatives with accurate information on realistic cost of currently covered treatments of congenital defects, the necessary treatments not currently covered by insurance, and additional out-of-pocket costs covered by patients. A concerted effort by plastic surgeons should provide tools for ELSA legislative champions to re-introduce and pass the bill during the 118th Congress. This process highlights the essential role of organized medicine in advocating for the successful passage of healthcare legislation.

Abstract B010: PITAR, a p53 inactivating TRIM28 associated RNA, executes an alternate way of p53 inactivation

Abstract B010: PITAR, a <i>p</i>53 inactivating TRIM28 associated RNA, executes an alternate way of p53 inactivation

Ab-initio Calculation of Electronic Properties of Doped GaN Nanoribbon

In this study, the electronic and magnetic properties of hydrogen-passivated armchair and zigzag GaN nanoribbons are investigated using density-functional theory. We explore that doped TM atom leads to additional levels in the band gap and thus plays an essential role in spin polarization and changes their properties. Depending on its location in the nanoribbon, the character of spin-dependent electronic property of a doped GaN-NR was analyzed. Our calculations show that, regardless of the position of Mn atoms, GaNNTs are stable in ferromagnetic phase.

Overview of the contributions from all lanthanide elements to kilonova opacity in the temperature range from 25000 to 40000 K

It is now well established that the neutron star (NS) merger is at the origin of the production of trans-iron heavy elements in the universe. These elements are therefore present in large quantities in the ejected matter, whose electromagnetic radiation, called kilonova, is characterized by a significant opacity due to the high density of spectral lines belonging to many heavy ions. Among these, the lanthanide ions play an essential role since, with their open 4f subshell, they have a considerable number of transitions that can absorb emitted light. The knowledge of the atomic structure and the radiative parameters of these ions as well as the determination of the corresponding opacities is therefore of paramount importance for the spectral analysis of kilonovae. The main goal of the present work is to determine the relative contributions of the different lanthanide elements to the opacity of the emission spectrum of a kilonova in its early phase, that is, a few hours after the NS merger, where the conditions are such that the temperature is between 25000 and 40000 K. At these temperatures, the lanthanide ions whose charge states are between V and VII are predominant. We used the pseudo-relativistic Hartree-Fock (HFR) method extensively to calculate the relevant atomic data (energy levels, wavelengths, and oscillator strengths) in La-Lu V-VII ions. The corresponding monochromatic opacities were estimated from the expansion formalism. We calculated the spectroscopic parameters for a total of more than 800 million radiative transitions in all the ions considered. These data were used to estimate the expansion opacities and Planck mean opacities for all the lanthanide elements at early-phase kilonova conditions between 25000 and 40000 K, making it possible to deduce the respective contributions of each element as a function of temperature. Atomic calculations were also carried out with the fully relativistic Multiconfiguration Dirac-Hartree-Fock (MCDHF) method in the specific case of the Yb V ion, as the available experimental data had not yet been compared with the theoretical calculations in our previous studies on lanthanide ions.

Study on multi-target effects of the novel HDAC6 inhibitor W5 on Aβ/Cu2+-induced Alzheimer's disease model of rats

Histone deacetylase 6 (HDAC6) is a key therapeutic target in neurodegenerative diseases such as Alzheimer’s disease (AD), which has been demonstrated to play an essential role in memory function and microtubule-associated tau physiology. In this study, W5 was used to treat AD model rats induced by Aβ/Cu2+ to study the improving effect of W5 on learning and memory impairment in AD rats and its related mechanism, to provide the basis for the subsequent development of W5 as an anti-AD drug. Results showed that W5 could decrease the expression of Aβ, Tau, and p-Tau proteins in the hippocampus of AD rats to inhibit the formation of senile plaques and neurofibrillary tangles, down-regulate the expression of Bax mRNA and Caspase-3 mRNA, and up-regulate the expression of Bcl-2 mRNA to reduce the apoptosis of neuron cells, reverse the expression of TNF-α, IL-1β and IL-6 mRNA to regulate neuroinflammatory response in AD rat brain. W5 also could regulate the oxidative stress state of AD rats, and balance the neurotransmitter disorder in AD rats' brain tissue. Overall, W5 could recover the morphology of hippocampal neurons and improve the learning and memory dysfunction in AD rats by regulating multiple targets in AD rats, providing a promising therapeutic avenue for the treatment of AD.

Mitochondrial targeting improves the selectivity of singlet-oxygen cleavable prodrugs in NMIBC treatment.

Mitochondria play an essential role in cancer treatment by providing apoptotic signals. Hexyl aminolevulinate, an FDA-approved diagnosis for non-muscle invasive bladder cancer, induces the production of protoporphyrin IX (PpIX) preferentially by mitochondria in cancer cells. Photosensitizer PpIX upon illumination can release active chemotherapy drugs from singlet oxygen-activatable prodrugs. Prodrugs placed close enough to PpIX formed in mitochondria can improve the antitumor efficiency of PpIX-PDT. The preferred uptake of prodrugs by cancer cells and tumors can further enhance the selective damage of cancer cells over non-cancer cells and surrounding normal tissues. Mitochondriotropic prodrugs of anticancer drugs, such as paclitaxel and SN-38, were synthesized using rhodamine, a mitochondrial-targeting moiety. Invitro, the mitochondrial targeting helped achieve preferential cellular uptake in cancer cells. In RT112 cells (human bladder cancer cells), intracellular prodrug concentrations were 2-3 times higher than the intracellular prodrug concentrations in BdEC cells (human bladder epithelial cells), after 2 h incubation. In an orthotopic rat bladder tumor model, mitochondria-targeted prodrugs achieved as much as 34 times higher prodrug diffusion in the tumor area compared to the nontumor bladder area. Overall, mitochondria targeting made prodrugs more effective in targeting cancer cells and tumors over non-tumor areas, thereby reducing nonspecific toxicity.

HMGA1 regulates the mitochondrial apoptosis pathway in sepsis-induced cardiomyopathy.

High mobility group protein AT-hook 1 (HMGA1), an architectural transcription factor, has previously been reportedto play an essential role in architectural remodeling processes. However, its effects on cardiovascular diseases, particularly sepsis-induced cardiomyopathy, have remained unclear. The study aimed to investigate the role of HMGA1 in lipopolysaccharide-induced cardiomyopathy. Mice subjected to lipopolysaccharide for 12 h resulted in cardiac dysfunction. We used an adeno-associated virus 9 delivery system to achieve cardiac-specific expression of the HMGA1 gene in the mice. H9c2 cardiomyocytes were infected with Ad-HMGA1 to overexpress HMGA1 or transfected with si-HMGA1 to knock down HMGA1. Echocardiography was applied to measure cardiac function. RT-PCR was used to detect the transcriptional level of inflammatory cytokines. CD45 and CD68 immunohistochemical staining were used to detect inflammatory cell infiltration and TUNEL staining to evaluate the cardiomyocyte apoptosis, MitoSox was used to detect mitochondrial reactive oxygen species, JC-1 was used todetect Mitochondrial membrane potential. Our findings revealed that the overexpression of HMGA1 exacerbated myocardial inflammation and apoptosis in response to lipopolysaccharide treatment. Additionally, we also observed that H9c2 cardiomyocytes with HMGA1 overexpression exhibited enhanced inflammation and apoptosis upon stimulation with lipopolysaccharide for 12 h. Conversely, HMGA1 knockdown in H9c2 cardiomyocytes attenuated lipopolysaccharide-induced cardiomyocyte inflammation and apoptosis. Further investigations into the molecular mechanisms underlying these effects showed that HMGA1 promoted lipopolysaccharide-induced mitochondrial-dependent cardiomyocyte apoptosis. The study reveals that HMGA1 worsens myocardial inflammation and apoptosis in response to lipopolysaccharide treatment. Mechanically, HMGA1 exerts its effects by regulating the mitochondria-dependent apoptotic pathway.

Efficacy of Omalizumab for the treatment of Bullous Pemphigoid. Spanish multicenter real-world experience.

Bullous Pemphigoid (BP) is the most common autoimmune blistering disease. Most patients are elderly and associate multiple comorbidities. Topical and systemic corticosteroids are considered as the first-line treatment for BP and immunosuppressors are used as steroid-sparing treatments but both have side effects and contraindications which are even more common in this elderly population. New treatments targeting interleukins and receptors related to BP pathogenesis have been proposed to decrease this side effects while achieving equal or better effectiveness response rates.Omalizumab is a monoclonal antibody that targets IgE that has been proposed for the treatment of BP due to the evidence that IgE autoantibodies play an essential role in BP pathogenesis. To assess the efficacy and security of Omalizumab for the treatment of BP, we carried out a multicenter, retrospective, observational study including patients diagnosed of BP who received omalizumab for at least 3 months from 15 tertiary hospitals in Spain. IgE levels prior to treatment was measured and we evaluate the possible correlation with clinical response. We excluded patients treated with Omalizumab for less than 3 months as we consider this duration is insufficient for a comprehensive assessment of its efficacy. To evaluate the effectiveness of the treatment we used the percentage of BSA improvement. We included 36 patients. The vast majority associate multiple comorbidities and all patients had used other systemic therapies apart from corticoids before Omalizumab.83% experienced some kind of treatment response and 42% of all patient treated achieved complete response.We did not find any correlation between higher levels and a better response (p=0,1791).All patients tolerated Omalizumab without reported side-effects. Omalizumab is a good therapeutic alternative for BP as it obtained clinical response in most patients and nearly half of the cases achieving complete response. It showed no side effects which is crucial in elderly patients suffering from BP.

Coordinated regulation of phosphatidylinositol 4-phosphate and phosphatidylserine levels by Osh4p and Osh5p is an essential regulatory mechanism in autophagy

Macroautophagy (hereafter autophagy) is an intracellular degradative pathway in budding yeast cells. Certain lipid types play essential roles in autophagy; yet the precise mechanisms regulating lipid composition during autophagy remain unknown. Here, we explored the role of the Osh family proteins in the modulating lipid composition during autophagy in budding yeast. Our results showed that osh1-osh7∆ deletions lead to autophagic dysfunction, with impaired GFP-Atg8 processing and the absence of autophagosomes and autophagic bodies in the cytosol and vacuole, respectively. Freeze-fracture electron microscopy (EM) revealed elevated phosphatidylinositol 4-phosphate (PtdIns(4)P) levels in cytoplasmic and luminal leaflets of autophagic bodies and vacuolar membranes in all deletion mutants. Phosphatidylserine (PtdSer) levels were significantly decreased in the autophagic bodies and vacuolar membranes in osh4∆ and osh5∆ mutants, whereas no significant changes were observed in other osh deletion mutants. Furthermore, we identified defects in autophagic processes in the osh4∆ and osh5∆ mutants, including rare autophagosome formation in the osh5∆ mutant and accumulation of autophagic bodies in the vacuole in the osh4∆ mutant, even in the absence of the proteinase inhibitor PMSF. These findings suggest that Osh4p and Osh5p play crucial roles in the transport of PtdSer to autophagic bodies and autophagosome membranes, respectively. The precise control of lipid composition in the membranes of autophagosomes and autophagic bodies by Osh4p and Osh5p represents an important regulatory mechanism in autophagy.

SafeNet: Towards mitigating replaceable unsafe Rust code via a recommendation‐based approach

AbstractRust is a system‐level programming language with advantages in memory safety. It ensures that any Rust programs without unsafe code should not incur undefined behaviours. However, unsafe code still plays an essential role in Rust to achieve low‐level control. Therefore, a major design pattern of Rust programs is interior unsafe, which wraps unsafe code as safe APIs and handles all undefined behaviours internally. Rust standard library already provides a rich set of safe APIs to facilitate Rust code development. Nevertheless, due to unfamiliarity with these APIs, developers may misuse unnecessary unsafe code and suffer memory‐safety risks. In this paper, we investigate an approach to mitigate replaceable unsafe code. We first analyse unsafe APIs of the Rust standard library and summarize their common usage patterns. Each pattern corresponds to one or several code samples in our knowledge base. Then, we develop an approach to automatically recognize the usage pattern and recommend corresponding code samples. Our approach leverages dataflow analysis to exclude impossible patterns and employs a BERT‐based machine learning model to find the most similar pattern among the rest. We have conducted evaluation experiments with 472 unsafe code snippets collected from GitHub projects and successfully recognized the pattern of 394 snippets. We hope our approach can assist developers in detecting unnecessary unsafe code and suggesting safe alternatives.

Properties and Behaviors of Heavy Quarkonia: Insights through Fractional Model and Topological Defects

In this study, we investigated the impact of a topological defect (λ) on the properties of heavy quarkonia using the extended Cornell potential. We solved the fractional radial Schrödinger equation (SE) using the extended Nikiforov-Uvarov (ENU) method to obtain the eigenvalues of energy, which allowed us to calculate the masses of charmonium and bottomonium. One significant observation was the splitting between nP and nD states, which attributed to the presence of the topological defect. We discovered that the excited states were divided into components corresponding to 2l+1, indicating that the gravity field induced by the topological defect interacts with energy levels like the Zeeman effect caused by a magnetic field. Additionally, we derived the wave function and calculated the root-mean radii for charmonium and bottomonium. A comparison with the classical models was performed, resulting in better results being obtained. Furthermore, we investigated the thermodynamic properties of charmonium and bottomonium, determining quantities such as energy, partition function, free energy, mean energy, specific heat, and entropy for P-states. The obtained results were found to be consistent with experimental data and previous works. In conclusion, the fractional model used in this work proved an essential role in understanding the various properties and behaviors of heavy quarkonia in the presence of topological defects.

Decoding the potential role of regulatory T cells in sepsis-induced immunosuppression.

Sepsis, a multiorgan dysfunction with high incidence and mortality, is caused by an imbalanced host-to-infection immune response. Organ-support therapy improves the early survival rate of sepsis patients. In the long term, those who survive the "cytokine storm" and its secondary damage usually show higher susceptibility to secondary infections and sepsis-induced immunosuppression, in which regulatory T cells (Tregs) are evidenced to play an essential role. However, the potential role and mechanism of Tregs in sepsis-induced immunosuppression remains elusive. In this review, we elucidate the role of different functional subpopulations of Tregs during sepsis and then review the mechanism of sepsis-induced immunosuppression from the aspects of regulatory characteristics, epigenetic modification, and immunometabolism of Tregs. Thoroughly understanding how Tregs impact the immune system during sepsis may shed light on preclinical research and help improve the translational value of sepsis immunotherapy.

The Effect Of “Sovereign Europe” Strategy on The Economic and Trade Relation Between China And EU

China and the EU are powerful international actors that play an essential role in the current international society. Economic and trade cooperation between China and the EU is a significant aspect of China-EU cooperation. However, China-EU relations have been facing several challenges since 2019. The concept of ‘sovereign Europe’ was implemented in 2020, in which the EU aims to gain ‘autonomy’ and ‘independence’. By using secondary data on China’s total trade value between 2019 and 2023, it is found that the total trade value between China and the EU shows an increasing trend before the end of 2021, apart from a decrease in the first half year of 2020, and starts to decrease since 2022. These trends are shown in several European countries, including France, Germany, and Spain. However, European countries such as Sweden and Estonia show a different trend. These trends show that the efforts to establish a ‘sovereign Europe’ did not cause the decline of China-EU economic cooperation until 2022 due to interdependence and the EU’s need to cooperate with China in combating the US’s influences in defense and finance. However, after 2022, the Russia-Ukraine conflict increases the EU’s concerns about geopolitical tension and, thus, views China more as a ‘threat’. As a result, the decline of the China-EU economic relation is likely due to the combined effect of building a ‘sovereign Europe’ and the Russia-Ukraine conflict.

DEAD Box RNA Helicases: Biochemical Properties, Role in RNA Processing and Ribosome Biogenesis.

DEAD box RNA helicases are a versatile group of ATP dependent enzymes that play an essential role in cellular processes like transcription, RNA processing, ribosome biogenesis and translation. These enzymes perform structural rearrangement of complex RNA molecules and enhance the productive folding of RNA and organization of macromolecular complexes. In this review article besides providing the outline about structural organization of helicases, an in-depth discussion will be done on the biochemical properties of RNA helicases like their substrate binding, binding and hydrolysis of ATP and related conformational changes that are important for functioning of the RNA helicase enzymes. I will extensively discuss the physiological role of RNA helicases in RNA processing and ribosome biogenesis.

Highly stretchable, durable, and reversibly thermochromic wrapped yarns induced by Joule heating: With an emphasis on parametric study of elastane drafts

Abstract Highly stretchable thermochromic wrapped yarns, which employ elastane filament (EF) as core, stainless steel wire, and thermochromic polyester filament as the first and second winding, was prepared, and the effect of elastane draft upon yarn properties was investigated. It was found that the elastane draft played an essential role in determining the final yarn behavior, and the optimized elastane draft parameter was 2.5 using Technique for Order Preference by Similarity to Ideal Solution. It is a distinctive configuration of yarn’s constituents and the EF draft that are responsible for the exceptional stretchability of yarns, and it showed mechanical robustness following cyclic stretch. Importantly, the yarn exhibited rapid, durable, and reversible color conversion when subjected to cyclic voltage, cyclic abrasion, and alkali. Finally, a flower-shaped pattern was fabricated by embroidering yarn onto an elastic substrate as a proof-of-concept, and no obvious variation of color fidelity was observed during the stretch.

School-based sexual and reproductive health education among adolescents in developing countries

The increase in risky sexual behavior among adolescents indicates the need to strengthen sexual and reproductive health. Schools have an essential role, but few studies are related to mapping models of school-based sex and reproductive education for adolescents in developing countries. This scoping review examines school-based sexual and reproductive health education among adolescents in developing countries. A systematic approach was used to search the literature through 5 electronic databases: Science Direct, EBSCO, PubMed, CrossReff, and Proquest. Subsequently, two reviewers conducted a conformity analysis with the inclusion/exclusion criteria, and then the search results that met the requirements were analyzed descriptively. A total of 14 articles met the inclusion criteria. The model of sexual health education in schools comprises digital-based education, peer groups, and comprehensive sex education to improve life skills. Barriers to implementation include culture, traditional norms, organization, commitment, and supporting resources. The supporting factors are student enthusiasm, exciting methods and media, community, and parental support. School-based sexual and reproductive education increases knowledge and attitudes about sexual health. Therefore, there is a need for a comprehensive sexual education model integrated into the school curriculum using attractive media and involving several well-organized stakeholders.

THE CONCEPT OF SOCIAL INNOVATIONS AND THEIR ROLE IN THE DEVELOPMENT OF SOCIETY

The article is devoted to the study of the concept of social innovations and their essential role in the development of modern society. The author analyzes the key aspects of social innovations, defines their essence and main characteristics. The article highlights various areas of application of social innovations, ranging from social entrepreneurship to technological solutions that contribute to solving social problems. Special attention is paid to the impact of social innovations on social change and sustainable development. The authors present examples of successful implementation of social innovations in various countries, emphasizing their importance for the formation of a more equitable and efficient society. The scientific article makes a valuable contribution to understanding the role of social innovation in modern socio-economic dynamics and provides practical recommendations for the development and support of social innovation initiatives.