Variability Of Essential Oils Research Articles

Chemical Variability and Chemotype Concept of Essential Oils from Algerian Wild Plants.

The chemical compositions of eleven wild species of aromatic and medicinal plants indigenous to Algeria, including Thymus, Mentha, Rosmarinus, Lavandula, and Eucalyptus, were analyzed. The identification of the chemical composition of each oil was conducted using GC-FID and GC-MS capillary gas chromatography. The study investigated the chemical variability of the essential oils based on several parameters. These included the impact of the vegetative cycle on oil composition, variations among subspecies of the same species, variations among species within the same genus, the influence of environmental factors on composition variations within a species, chemo typing, and the genetic factors (such as hybridization) contributing to chemical variability. The concepts of chemotaxonomy, chemotype, and chemical markers were examined to understand their limitations and emphasize the importance of regulating the use of essential oils derived from wild plants. The study advocates for an approach that involves the domestication of wild plants and screening their chemical compositions according to more specific standards for each commercially available oil. Lastly, the nutritional implications and the variability of nutritional impact based on the chemical composition of the essential oils will be discussed.

Variations in Essential Oils from the Leaves of Cinnamomum bodinieri in China.

Cinnamomum plants are rich in natural essential oils, which are widely used as materials in the fragrance, insecticidal, antibacterial agent, pharmaceutical, and food industries; however, few studies have investigated the essential oil components of Cinnamomum bodinieri. Therefore, this study investigated the diversity of essential oils from the leaves of 885 individual C. bodinieri plants across 32 populations in five provinces. Essential oils were extracted by hydrodistillation, and then qualitative and quantitative analyses of the compounds were performed by GC-MS and GC-FID. A total of 87 chemical constituents were identified in the essential oils, including 33 monoterpenes, 48 sesquiterpenes, and six other compounds. The average oil yield was 0.75%, and individual oil yields ranged from 0.01% to 4.28%. A total of 16 chemotypes were classified according to variations in the essential oil chemical constituents of C. bodinieri, among which the camphor-type, citral-type, and eucalyptol-type were dominant. Moreover, the borneol-type, cymol-type, elemol-type, methylisoeugenol-type, and selina-6-en-4-ol-type were reported in C. bodinieri for the first time. The yield and principal components of the essential oils were mainly affected by altitude, temperature, and sunshine duration, among which altitude had the most significant effect; thus, low-altitude areas are more suitable for the synthesis and accumulation of essential oils. Based on the different characteristics of the essential oils in the leaves of C. bodinieri, several excellent populations and individuals were identified in this study. Moreover, the findings provide a foundation for breeding superior varieties and studying essential oil biosynthesis mechanisms in the future.

Essential Oil Variability of Azorean Cryptomeria japonica Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality

This study reports on the in vivo molluscicidal activity and Artemia salina lethality of Azorean Cryptomeria japonica leaf (CJL) essential oils (EOs) obtained by hydrodistillation (HD) and water-steam distillation (WSD) techniques, especially in light of the recent focus on the use of forestry and wood industry residues in a sustainable world economy. Molluscicidal activity was performed during several life stages of Radix peregra by the immersion method, under laboratory conditions. A first screening through a single-dose bioassay revealed that both EOs were highly active towards eggs, juveniles and adult snails (ca. 100% mortality). Concentration- and time-toxicity assays were carried out only on adult snails to determine the lethal parameters (LC50;90 and LT50;90). The LC50 values were 33 and 62 µg/mL for EO–WSD and EO–HD, respectively, after 48 h. The LT50 of EO–WSD required only 21 h for both 16 h and continous exposure periods, while that of EO–HD was slighty superior (21.8–25.6 h). Although not significant, EO–WSD was also slightly more toxic against A. salina than EO–HD (LC50 = 98 and 115 µg/mL after 24 h, respectively). In conclusion, Azorean CJL EOs, which are rich in α-pinene (mostly EO–WSD), have huge potential to be used as safe raw materials for the development of natural molluscicide products to control snails responsible for transmitting fascioliasis.

Effect of Foliar Treatments with Calcium and Nitrogen on Oregano Yield

Oregano, Origanum vulgare L., is a perennial herbaceous plant belonging to the Lamiaceae family. Oregano shows variations in biomass yield and essential oil (EO) content due to the influence of abiotic and biotic factors. The aim of this study was to assess the effect of different foliar applications based on calcium (Ca) and nitrogen (N) on morphological and productive parameters in oregano. Tests were carried out in Sicily (Italy) in 2020–2021. In each year, eight foliar applications were applied. Only flowers and leaves were used for the extraction of the EO. For all parameters in the study, except for plant height and inflorescence length, the highest values were found in treated plants with respect to the control. In plants treated with calcium and nitrogen, an increase of between 0.6 and 1.6 t ha−1 was observed for fresh yield, and an increase of between 0.5 and 0.9 t ha−1 was observed for dry yield. The increase in biomass yield led to an increase in EO yield of between 4 and 12 kg ha−1. The results highlight that foliar treatments with Ca in combination with N enable growth in crop production in environments that show poor water availability.

GC-TOF-MS-based metabolomics correlated with bioactivity assays unveiled seasonal variations in leaf essential oils of two species in Garcinia L.

The genus Garcinia L. is known for its many practical uses, particularly as medicines and food. However, few studies have reported the seasonal influence on Garcinia metabolites. The objective of this study was to evaluate the seasonal effects on the chemical composition and bioactivity of Garcinia essential oils (EOs), using the native G. xanthochymus Hook.f. and endemic G. yunnanensis Hu in southwest China. Gas chromatography time-of-flight mass spectrometry-based metabolomics uncovered qualitative and quantitative variations of 118 EO compounds, mainly comprising sesquiterpene hydrocarbons, oxygenated sesquiterpenes and esters. Chemometric approaches, including principal component analysis and partial least-squares-discriminant analysis (PLS-DA), further indicated that EO profiles varied distinctly according to seasons. From PLS-DA models built on seasonal samples, a total of 15 differential compounds, such as ethyl palmitate and β-bisabolene, were characterized from G. xanthochymus and 19 compounds, including pentadecane and hexadecanoic acid, from G. yunnanensis. Biological assays demonstrated that the bioactivities of leaf EOs from both species were also notably affected by seasonality. The most promising season to harvest Garcinia leaves for their cytotoxic and anti-inflammatory EOs is winter, with IC50 values lower than 20 μg/mL; for antibacterial, it is spring with MIC50 values lower than 135 μg/mL against Listeria monocytogenes. Correlation analysis further indicated that some EOs, such as β-copaene, β-bisabolene and hexadecanoic acid, were positively associated with the observed bioactivity results. These findings, taken together, suggest that EOs from Garcinia leaves have strong biological activities, but their seasonal variation needs to be carefully considered for future product development.

Chemical composition and pesticide activity of essential oils from Artemisia annua L. harvested in the rainy and winter seasons

Artemisia annua L. [family Asteraceae (Compositae)] is a well-known medicinal herb with a broad spectrum of applications in pharmaceuticals and cosmetics. The present study aimed to investigate the variation of essential oil (EO) composition from the plant aerial parts, harvested in rainy (AAREO) and winter (AAWEO) seasons. GC-MS analysis of AAREO and AAWEO led to the identification of 75 and 59 constituents, respectively. AAREO was dominated by camphor (14.1%), germacrene-D (9.0%), β-caryophyllene (8.7%), and eucalyptol, (4.2%) whereas AAWEO was abundant in camphor (17.9%), eucalyptol (14.6%) and artemisia alcohol (3.5%). Both EOs showed antifeedant activity against Spodoptera litura and nematocidal activity versus Meloidogyne incognita. The in silico test proved that the major EOs compounds showed a good score of docking with acetylcholinesterase (PDB ID: 2ACE) compared to carbofuran.

Exploring the Ecological Preferences and Essential Oil Variability in Wild-Growing Populations of the Endangered Local Greek Endemic Thymus holosericeus (Lamiaceae).

Thymus holosericeus Čelak. (Lamiaceae) is a taxonomically isolated and endangered local endemic thyme species which is geographically isolated in four Ionian Islands (West Greece). The present study investigated the essential oil (EO) composition, the ecological preferences, and their correlations in three T. holosericeus wild-growing populations from Zakynthos (ΤH-Z), Cephalonia (ΤH-C) and Lefkada (ΤH-L). The variations in essential oil yield and the composition of T. holosericeus populations were evaluated using hydrodistillation, GC/MS, TLC and NMR analysis. The climatic conditions of each sample were organized and analyzed in RStudio with the raster package and in SPSS with Pearson's Canonical Correlation Analysis (CCA), respectively. The aerial parts of the plants varied in EO yields from 1.92 to 2.28% w/v. The analysis of EO constituents revealed noteworthy qualitative and quantitative inter-population variation. The composition of EOs revealed the presence of linalool (82.77%) and borneol (5.95%) as major compounds in ΤH-Z, while carvacrol (35.34%), geraniol (23.98%), linalool (14.37%), borneol (5.66%), thymol (4.27%) and p-cymene (4.08%) were the main compounds in ΤH-C and linalool (40.37%), geraniol (39.42%) and borneol (5.20%) were dominant components in ΤH-L. The results of the gas chromatography procedure have also been confirmed by 1H and 13C-NMR and TLC analysis. The ecological profile showed an average annual precipitation of 942 ± 18.33 mm and the temperature limits in which T. holosericeus seems to adapt to are 6.80± 1.08 °C 27.70 ± 0.70 °C. Regarding the examined samples, TH-C was adapted to the driest summer and coldest winter conditions, TH-Z was adapted to the lowest annual precipitation with the most complex climatic conditions, and TH-L was adapted to the highest summer temperatures with the lowest precipitation in the wettest period of the year. For each sampled population, the CCA identified the association of the samples' EOs composition with the prevailing local environmental conditions.

Variations in Yield, Essential Oil, and Salicylates of Filipendula ulmaria Inflorescences at Different Blooming Stages.

Meadowsweet (Filipendula ulmaria) is an essential oil-bearing, pharmacologically valuable medicinal plant growing wild in floodlands that are either not usually used for agriculture or have low economic value. The aim of this work was to understand the relationship between the yield of F. ulmaria inflorescences and the qualitative and quantitative composition of the essential oil during flowering stages in different habitats. Five different habitats of F. ulmaria were investigated for evaluation of inflorescence characteristics (length and weight) and the quantitative and qualitative composition of essential oils in early and late blooming stages (growing stage BBCH 62-63 and BBCH 65-67/72-73, respectively). The results showed significant (p < 0.05) positive correlations between the length and weight of inflorescences both in early and in late blooming stages (r = 0.73 and r = 0.72, respectively). The early blooming stage was observed to have 1.2-2 times greater quantity of volatile oils in comparison with the late blooming stage. Salicylaldehyde and methylsalicylate were the main salicylates in both blooming stages. Spearman's correlation showed statistically significant relationship between percentages of salicylaldehyde and methylsalicylate (r = -0.94, p < 0.05). An increase in salicylaldehyde was accompanied by a decrease in methylsalicylate in essential oils. Statistically significant relations between the investigated parameters of plant and the parameters of habitat were not detected. The dried raw material yield of F. ulmaria in 1 ha in the late blooming stage was 18-56% higher than in the early blooming stage; however, the yield of essential oil in the early flowering stage was 1.5-1.6 times higher than in the late flowering stage. The obtained research data suggest that the low economic value of floodlands-the habitats of F. ulmaria-can be increased by using these areas as natural industrial plots.

Essential Oil Content and Composition of the Chamomile Inflorescences (Matricaria recutita L.) Belonging to Central Albania

The chamomile drug (Chamomillae anthodium) is widely known and has been used as a medicine for ages. Today, the drug is officially registered in the European Pharmacopoeia. Despite the economic importance of the chamomile Matricaria recutita L., little is known about the extent and nature of the essential oil variability and composition of this species in Albania. Therefore, information about the extent of the uses of various gene pools is extremely valuable for the development of future chamomile cultivation and breeding programs. This study aimed to analyze the differences among 29 populations in different sites in central Albania. The quantities of essential oils ranged from a low of 0.10 ± 0.01% in Fier and Tirana to a high of 0.75 ± 0.05% in Linzȅ. The yield of volatile oils depended on the geography, altitude, and other factors, including stress influences on the site of plant population growth. This fact was confirmed by various subclimatic characteristics obtained from individual localities. Essential oil extracted from chamomile inflorescences was recorded to have between 23 and 43 chemical components. It was found that /-/-α-bisabololoxides B and A were the major constituents in 25 samples, with only 4 having dominant /-/-α-bisabolol. The uniquely determined chemical type of the chamomile wild populations in Albania was chemical type B (/-/-α-bisabololoxide B > /-/-α-bisabololoxide A > /-/-α-bisabolol). Based on the study of chamomile’s pharmacodynamic properties, the sesquiterpenes /-/-α-bisabolol and chamazulene are considered to be the most valuable constituents. Consequently, a very intensive improvement breeding program must begin, with emphasis on drug yield, polyploidization, essential oil quantity, and both component contents in the oil.

Assessment of yield and chemotypic variation of essential oils from the seed orchard of Corymbia citriodora.

Assessment of yield and chemotypic variation of essential oils from the seed orchard of Corymbia citriodora.

Volatile Chemical Variation of Essential Oils and Their Correlation with Insects, Phenology, Ontogeny and Microclimate: Piper mollicomum Kunth, a Case of Study.

The aim of this study was to monitor the volatile chemical composition from leaves and reproductive organs of Piper mollicomum Kunth (PM), in its reproduction period, as well as register inflorescence visitors, microclimate and phenological information. The essential oils (EOs) obtained from the different fresh organs by hydrodistillation were identified and quantified by Gas Chromatography/Mass Spectrometry (GC/MS) and by GC coupled to a Flame Ionization Detector (GC/FID), respectively. The cercentage content of some volatiles present in reproductive organs, such as limonene, 1,8-cineole, linalool and eupatoriochromene, increased during the maturation period of the inflorescences, and decreased during the fruiting period, suggesting a defense/attraction activities. Furtermore, a biosynthetic dichotomy between 1,8-cineole (leaves) and linalool (reproductive organs) was recorded. A high frequency of bee visits was registered weekly, and some correlations showed a positive relationship between this variable and terpenes. Microclimate has an impact on this species' phenological cycles and insect visiting behavior. All correlations between volatiles, insects, phenology and microclimate allowed us to present important data about the complex information network in PM. These results are extremely relevant for the understanding of the mechanisms of chemical-ecological plant-insect interactions in Piperaceae, a basal angiosperm.

Kaffir Lime Essential Oil Variation in the Last Fifty Years: A Meta-Analysis of Plant Origins, Plant Parts and Extraction Methods

Kaffir lime has been widely researched for use as an essential oil, alongside its main function as an Asian spice, due to the needs of numerous industries. A meta-analysis was used to summarize the variation in yield and main components of kaffir lime essential oils in response to differences in plant origins, plant parts, and extraction methods during the last fifty years. A database was constructed from 85 data items from 36 single studies, prepared by following PRISMA-P. The result showed no significant effect of extraction method on variation in oil yield and main components. In contrast, after integrating numerous single studies under a statistical approach, some interesting facts emerged, such as (i) plant origin significantly affected the citronellol, i.e., subtropical conditions were more favorable than tropical ones; (ii) caryophyllene was found in all countries, from subtropical America and tropical Asia to tropical East Africa; (iii) the richest parts of citronellal, citronellol, citronellyl acetate, and sabinene were leaves, blossoms, twigs, and fruit peels, respectively; and (iv) due to significant interaction of plant origin and plant parts, a very challenging issue in boosting oil yield could be answered by formulating location-specific and organ-specific culture practices. This study had succeeded in providing further research directions.

Seasonal variation of essential oil of germplasm of Lippia origanoides Kunth. (Verbenaceae)

The biological activities attributed to Lippia origanoidesKunth. vary according to the chemical composition of its essential oil, which can be related to weather factors. The aim of this research was the seasonal study of the essential oil chemical composition taken from L. origanoidesaccessions maintained in germplasm bank in vivo. The essential oil was extracted by hydrodistillation in Clevenger apparatus and identification of the components was done by GC-MS. We have identified 15 compounds of which carvacrol, p-cymene, thymol and methyl-ether-thymol were majority throughout the year for most accessions. In three accessions showed fluctuations in the production of the major compound during the year like the α-pinene, limonene and borneol. The essential oil showed variation in chemical composition throughout the year, the major compound in most of the accessions during the year is carvacrol. In some accessions, there are alterations of the majority component.

Essential Oil Variability of Superior Myrtle (Myrtus communis L.) Accessions Grown under the Same Conditions.

Myrtle (Myrtus communis L., Myrtaceae) has numerous applications in pharmacology, food technology, and cosmetic industry. The current research aimed at measuring variations in the leaf essential oil (EO) compositions of 14 superior myrtle accessions originating in natural habitats of south Iran. The plants were grown under greenhouse conditions. Fresh leaf samples were harvested in June 2021. Based on dry matter, the extractable amount of EO in the accessions ranged from 0.42% (BN2) to 2.6% (BN5). According to GC/MS analysis, the major compounds in the EO were α-pinene (2.35-53.09%), linalyl acetate (0-45.3%), caryophyllene oxide (0.97-21.8%), germacrene D (0-19.19%), α-humulene (0-18.97%), 1,8-cineole (0-18.0%), limonene (0-17.4%), and p-cymene (0-13.2%). These myrtle accessions were classified into four groups, including I: caryophyllene oxide/germacrene D/α-humulene/methyl eugenol chemotype; II: α-pinene/p-cymene/α-humulene and (E)-β-caryophyllene; III: α-pinene/1,8-cineole, and linalool; IV: linalyl acetate/γ-terpinene/1,8, cineole/limonene. These classifications were established by considering the main EO components using hierarchical cluster analysis (HCA) and principal component analysis (PCA). In summary, this study provided new insights into available opportunities of selecting suitable genotypes for commercial cultivation purposes and planning breeding programs in the future.

Effect of glutathione and α‐tocopherol on coriander green fruits essential oil

AbstractCoriander essential oil has several biological characters those make it an important place as a source of natural product. Due to the lack of natural products, many essential oil compounds are imitated by others made of cheap chemicals, which studies have shown to have a great danger to human health. From this point of view, it is necessary to search for new ways to increase natural products, the most important of which are essential oils. One of these methods is the use of bioregulators to modify the production of essential oil and its components to increase its biological use area. Thus, the variations of coriander green fruits essential oil were evaluated under different rates of glutathione and α‐tocopherol to determine the possibility of using them in the production of essential oil from coriander green fruits. Coriander plants were treated during cultivation at rates of glutathione (0, 0.2, 0.4 and 0.6 g L−1) without or with α‐tocopherol (0.4 g L−1). Green fruits essential oil was separated by hydro‐distillation and analysed by gas chromatography and gas chromatography/Mass spectrometry; data were statistically analysed by two ways analysis of variance. Plants treated with 0.6 g L−1 glutathione with α‐tocopherol gave the maximum values of green fruits yield and essential oil content (% and yield per plant). The major essential oil constituents were linalool, undecan‐3‐ol and yomogi alcohol; while oxygenated monoterpenes were the main fractions. Plants exposed to glutathione with α‐tocopherol doses resulted in higher values of major components and main fraction than those subjected to glutathione rates. This trial ascertained that the subordination of coriander plants to glutathione and α‐tocopherol rates led to variations in green fruits essential oil, and this, in turn, affected its biological activities.

Essential Oil Variability in Iranian Populations of Heracleum persicum Desf. ex Fischer: A Rich Source of Hexyl Butyrate and Octyl Acetate.

Heracleum persicum Desf. ex Fischer seeds are a rich source of essential oils (EOs) with high antimicrobial and antioxidant effects. In order to determine the phytochemical variability in various Iranian H. persicum populations, seed samples were collected from 10 different climatic locations. The current study indicated that hexyl butyrate (20.9–44.7%), octyl acetate (11.2–20.3%), hexyl-2-methylbutyrate (4.81–8.64%), and octyl 2-methyl butyrate (3.41–8.91%) were the major components of the EOs. The maximum (44.7%) and the minimum (20.9%) content of hexyl butyrate were obtained from Kaleibar and Sari populations, respectively. Moreover, the octyl acetate content ranged from 2% (in Mahdasht) to 20.3% in Torghabeh population. The CA and PCA analysis divided the 10 Iranian H. persicum populations into three major groups. Populations from Khanghah, Kaleibar, Shebeilo, Showt, Mahdasht, and Amin Abbad showed a distinct separation in comparison with the other populations, having high contents of hexyl butyrate (39.8%) and low contents of octyl acetate (13.5%) (Chemotype II). According to correlation analysis, the highest correlation coefficient was among habitat elevation and hexyl butyrate content. In addition, the mean annual precipitation was negatively correlated with the content of hexyl butyrate. Although octyl acetate content showed high correlation with soil EC and mean annual temperature, it was not statistically significant. In general, in order to have plants with a high content of hexyl butyrate, it is recommended to harvest these plants from regions with high altitude and low rainfall such as Kaleibar.

Investigation on pollination approaches, reproductive biology and essential oil variation during floral development in German chamomile (Matricaria chamomilla L.)

German chamomile is an important medicinal and aromatic herb known for its blue essential oil. It lacks studies on anthesis, breeding systems and floral development with their impact on the essential oil. Therefore, the study investigated floral development and divided it into six reproductive stages (RS-1 to RS-6). The first four stages (5–6 days long) were identified as the floral enlargement and differentiation, followed by the fifth stage (10 days long) of three anthesis flushes, i.e., anther dehiscence, ray and disc florets' style branches flush. Anther dehiscence started 1–2 days before style branches flushes showed protandry and overlapped later with style branches flushes. Pollen production started from RS-3 and showed maximum viability (89%) at anther dehiscence (RS-5.1). Pollen showed dispersal through the air up to 0.7 m distance. Seed setting in controlled pollination experiments showed that removing disc florets could be successfully used as the emasculation alternate in German chamomile. The maximum essential oil content (0.40%) at the full blossomed floral stage (RS-4 &-5) suggested the right time for capitula harvesting. The findings on reproductive biology and breeding systems would offer several tools and techniques to support future breeding programs for genetic improvement of German chamomile.

English

The chemical composition and seasonal variation of essential oils (EO) extracted from the aerial parts of the traditional medicinal plant Piper rivinoides were analyzed using gas chromatography (GC) coupled with mass spectrometry (MS) and GC coupled with flame ionization detector (GC-FID) technique, respectively. The wild plants were collected from two different sites in the Atlantic Forest. The analysis allowed us to identify 96.60 to 99.80% of the EO composition. The major compounds with the highest relative percentage for both specimens, regardless of the season, were the bioactive monoterpenes α-pinene and β-pinene, which ranged from 34.78 (summer) to 53.87% (winter) and from 15.24% (autumn) to 47.71% (winter), respectively. The seasonal stability of the major compounds in the two study specimens throughout the year indicates that the phenological cycle influences biosynthesis more than abiotic factors. This type of chemical phenotypic stability is rarely observed in species belonging to the Piperaceae family, which is characterized by high chemical variability. Furthermore, this stability is favorable, and P. rivinoides has the potential to be a source of bioactive compounds. Key words: -pinene, -pinene, Atlantic Forest, aromatic plant, volatile compounds.

Essential oil variability in Echinophora cinerea Boiss. wild populations: a narrow- endemic and vulnerable species in Iran

ABSTRACT Echinophora cinerea Boiss. is an vulnerable plant species which is applied as gastrointestinal invigorator and stimulants in traditional medicine. A study was done to investigate the variation of essential oil (EO) composition of this species among seven wild populations distributed in various microclimates of Zagros Mountain, Iran. Based on the gas chromatography analyses, twenty-one constituents were monitored including 98.6–99.2% of the EO compositions. The major chemical components of essential oils were α-phellandrene (23.7–36.2%), α-pinene (9.5–24.8%), β-myrcene (3.3–17.5%), linalool (1.8–6.7%), β-phellandrene (3.4–5.5%), methyl eugenol (1.1–15.1%), citronellol (0.5–6.4%) and verbenone (1.9–4.9%). Classification of populations revealed four main chemotypes. There were a positive and significant correlation between soil organic carbon and α-terpineol and a negative correlation between α-phellandrene and β-phellandrene contents and the soil EC. The observed variability among the studied populations can provide the best source of materials with favorable components for industrial uses and pharmaceutical industries.

Comparative study of chemical variability of essential oils from the leaves of Lippia multiflora Mold (Verbenaceae) collected in five regions of Côte d'Ivoire

Chemical variability of a plant from various continents or collected in different localities justifies its different biological activities. Thus, the present study was undertaken to evaluate the statistical study of chemical variability of essential oils from leaves of Lippia multiflora Mold from five localities in the center of Côte d'Ivoire. Essential oils have been extracted by hydrodistillation with yields varying from 0.34 to 1.20% depending on localities. Analysis of their chemical compositions showed the predominance of eucalyptol / germacrene D (13.91 / 11.02%), α-citral / β-citral / α-phellandrene (21.87 / 16.74 / 12.79%), linalool / (±)-trans-nerolidol / α-citral (19.99 / 18.68 / 12.10%), α-citral / β-citral (17.20 / 11.59%) and α-citral / β-citral (16.38 / 11.57%) in the oils from Yamoussoukro, Tiébissou, Bouaflé, Toumodi and Dimbokro, respectively. Principal Component Analysis and Hierarchical Ascending Classification revealed that these essential oils can be classified in three groups or chemotypes dominated by Eucalyptol, Citral / Linalool and trans-Geraniol.