Essential Oil Solution Research Articles

Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis.

Aim:The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis.Materials and Methods:The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline), Group 2 (n=5): Amoxicillin alone (3 ml), and Group 3 (n=10): Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]). All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0) and at 24 (T24) and 48 (T48) h after the last treatment.Results:The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (p<0.05) at T24 and T48 h in sage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (p<0.05) in sage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations.Conclusion:In this study, the intramammary infusion of sage EO to ewes affected with subclinical mastitis resulted in a significant decrease in SCC 24 h and 48 h posttreatment. In addition, milk fat and lactose were increased in animals that received the EO as well as in those treated with the antibiotic.

AKTIVITAS ANTIBAKTERI DARI DESAIN MIKROEMULSI MINYAK ATSIRI KAYU MANIS

&lt;span class="fontstyle0"&gt;This research focused on the study of the capability of cinnamon essential oil as antibacterial&lt;br /&gt;agent, when it is made as a microemulsion solution. The study occupied further research on&lt;br /&gt;specific type, concentration, and amount of emulsifier needed to make the cinnamon essential&lt;br /&gt;oil miscible in water without causing destruction in its antibacterial activity. Four different&lt;br /&gt;emulsifiers were tested, namely Tween 20, Tween 80, soya lechitin, and carboxymethyl cellulose&lt;br /&gt;(CMC) to stabilize the essential oil in water. The emulsifiers were used at 2% w/w, while the&lt;br /&gt;essential oil concentrations were varied at 0.5%, 1%, and 1.5%. In this study, the antibacterial&lt;br /&gt;activity of the microemulsion solution of cinnamon essential oil was tested against two types of&lt;br /&gt;pathogenic bacteria commonly found in foods, namely Staphylococcus aureus and Escherichia&lt;br /&gt;coli. Among the four types of emulsifiers, which are used, CMC showed the best results as an&lt;br /&gt;emulsifier. The results of this study indicated that microemulsion solution has antibacterial&lt;br /&gt;activity best is a combination between the CMC and the essential oil of cinnamon&lt;/span&gt;

Sustainable fragrance cum antimicrobial finishing on cotton: Indigenous essential oil

Fragrance finishing of textile has been enormously increased and used in domestic and industrial application. Fragrance can be synthesis chemically but available in natural and inorganic. The investigation of this research is to study the combined effect of fragrance and antimicrobial finishing on cotton fabric by lavender essential oil with the use of β-Cyclodextrin, Chitosan citrate and β-Cyclodextrin/Grafted Chitosan through pad-dry method. Fourier Transform Infrared spectroscopy (FTIR) was used to study the formation of ester bonds between β-cyclodextrin/grafted chitosan and cotton celluloses. For all the finished fabric samples fragrance release rate performance and antimicrobial properties were measure by standard test methods. The results revealed that β-CD was highly soluble in 0.6gpl NaOH solution and 80gpl β-CD and 6% essential lavender oil solutions were found to be a most suitable combination for fragrance and antimicrobial finishing. FTIR studies reveal about the formation of a carboxylic ester between cotton and β-Cyclodextrin/Grafted Chitosan at 1730cm−1 ester peak.

The Essential Oil of Monarda didyma L. (Lamiaceae) Exerts Phytotoxic Activity in Vitro against Various Weed Seed.

The chemical composition of the essential oil of the flowering aerial parts of Monarda didyma L. cultivated in central Italy was analyzed by Gas Chromatography/Mass Spectrometry (GC/MS). The major compounds of the oil were thymol (59.3%), p-cymene (10.3%), terpinolene (9.2%), δ-3-carene (4.4%), myrcene (3.7%), and camphene (3.4%). The essential oil was tested in vitro for its anti-germination activity against Papaver rhoeas L., Taraxacum officinale F. H. Wigg., Avena fatua L., Raphanus sativus L. and Lepidium sativum L. seeds, demonstrating good inhibitory activity in a dose-dependent way. The exposure of the employed weed seeds to M. didyma essential oil and thymol solution (59.3%) increased the level of hydrogen peroxide (H2O2) and malondialdehyde (MDA), markers of oxidative stress, in emerging 5-day-old rootlets.

Seed treatments with essential oils protect radish seedlings against drought

Establishment of seedlings of economic crops is often reduced if there is not a steady supply of water. Essential oils (EO) from plants are increasingly used instead of synthetic chemicals to protect plant and animal products against biotic and abiotic stresses. We investigated priming radish seeds by soaking or by matriconditioning with synthetic or natural compounds as a means of inducing resistance to drought stress, thus maintaining crop yield. Priming radish seeds for two hours in solutions of essential oils (EO) thymol and carvacrol derived from Origanum syriacum, with “oregano natural product” (ONP; a solution of the residue remaining after EO extraction), or with the gibberellin synthesis inhibitor trinexapac ethyl (TE), was much more effective in inducing drought resistance than was matriconditioning with the same compounds in sawdust for two days. The latter treatment induced considerable fungal and bacterial infection in treated seeds if the substrate-matrix was not heat-treated beforehand. The increase in specific leaf area in plants from treated seeds was mostly consistent with an increase in leaf water content. Seed treatments with EO, ONP, and especially TE led to a three-fold increase in radish seedling survival compared with water-treated controls, when 21 day-old seedlings were irrigated after 6 days of drought. Under drought conditions, seedlings from treated seeds had a 2–3-fold increase in relative water content increased 2–3-fold, while membrane permeability decreased 20–50-fold as a result of the treatments. However, the physical benefits of the treatments often did not correlate with treatment-induced increases in physiological parameters such as pigments (chlorophyll, carotenoid, anthocyanin), pigment ratios (chlorophyll a/b, carotenoid/chlorophyll), or antioxidant activity. Seed treatments with biostimulants can be as effective as treatments with synthetic compounds in inducing drought resistance in seedlings.

Bioactivity of selected essential oils from medicinal plants found in Fiji against the Spiralling whiteflies ( Aleurodicus dispersus Russell)

The concentration of essential oil solutions [0.25%, 0.5% and 5% (v/v)] of five medicinal plants found in the South Pacific (Fiji) were subjected to the whitefly (Aleurodicus dispersus Russell) in order to assess eventual control activities, by both fumigant and repellent tests. The essential oil of Ocimum tenuiflorum L. exhibited the strongest fumigant activity against the Spiralling whiteflies with an LC50 value of 0.003% followed by the essential oil of Cymbopogon citratus (DC.) Stapf. (LC50 = 0.004%), Cananga odorata (Lam.) Hook F. and Thoms (LC50 = 0.050%), Murraya koenigii (L.) Spreng. (LC50 = 0.113%), and Euodia hortensis forma hortensis (LC50 = 0.114%). The essential oil of M. koenigii (RI=52%) and C. citratus (RI=52%) at 5% (v/v) concentration were found to have a higher repellent toxicity against the Spiralling whiteflies. The chemical composition of the selected essential oils was also determined using GC-MS. The trend in the chemical constituent of essential oils revealed that the phenolic and alcoholic compounds were the major groups of contributors to the tested activities. Thus, these data suggested that essential oils from the selected medicinal plants found in the South Pacific (Fiji) have the potential to be employed in the pesticidal activities.

Quality preservation of deliberately contaminated milk using thyme free and nanoemulsified essential oils

The objective of this study is to evaluate the effect of either a solution of Thymus capitatus essential oil or its nanoemulsion on the quality of milk contaminated by bacteria. After 24h of S. aureus inoculation, bacterial growth reached 202×103CFU/ml in the presence of the essential oil while it was limited to 132×103CFU/ml when treated with nanoemulsion. The reduction of antioxidant capacity of milk treated with essential oil was higher when treated with nanoemulsion. Moreover, free essential oil was more efficient in protecting proteins from degradation than the nanoemulsion. For instance, after 24h of E. hirae contamination, 26% of the total proteins were consumed in the presence of nano-encapsulated essential oil, while only 14% of the initial content was consumed when free essential oil was added. Concerning milk acidity increase and the inhibition of peroxide production, no statistical differences have been recorded between the use of free essential oil or its nano-emulsion. In conclusion, bulk or nano-encapsulated T. capitatus essential oil preserve milk quality and can extend its shelf life.

Matrix Effect on the Spray Drying Nanoencapsulation of Lippia sidoides Essential Oil in Chitosan-Native Gum Blends.

Essential oils have many applications in the pharmaceutical, chemical, and food fields, however, their use is limited to the fact that they are very labile, requiring their a priori encapsulation, aiming to preserve their properties.This work reports on the preparation of chitosan-gum nanoparticles loaded with thymol containing Lippia sidoides essential oil, using exudates of Anacardium Occidentale (cashew gum), Sterculia striata (chichá gum), and Anadenanthera macrocarpa trees (angico gum). Nanoparticles were produced by spray drying an emulsion of L.sidoides essential oil and aqueous solution of gums with different chitosan : gum ratios. Samples were characterized by FTIR and UV/VIS spectroscopy, particle size, volume distribution, and zeta potential. The FTIR spectrum showed the main signals of chitosan and the gums. Data obtained revealed that the samples had sizes in the nano range, varying from 17 nm to 800 nm. The zeta potential varied from + 30 mV to - 40 mV. Nanoparticle loading values varied from 6.7 % to 15.6 %, with an average encapsulating efficiency of 62 %, where the samples with high ratios of cashew gum and chichá gum presented high oil loading values. The data revealed that both the chitosan : gum ratio and polysaccharide characteristics play major roles in nanoencapsulation processes.

Dolabella‐3,7,18‐triene, the main constituent of the essential oil of the white lotus flower (Nymphaea lotus, Nymphaeaceae)

AbstractThis is the first report on the chemical composition of the essential oil of the flowers of the white lotus (Nymphaea lotus, Nymphaeaceae). The relative configuration of its main constituent, the diterpenoid dolabella‐3,7,18‐triene, was determined to be (1R,3E,7E,11S,12S) on the basis of DFT DP4 13C‐NMR quantitative chemical shift analysis. Both the essential oil (LC50 &lt; 50 ppm) and aqueous solution (55% larvae mortality in 50% (v/v) concentration) obtained through hydrodistillation extraction exhibited a satisfactory larvicidal effect against Aedes aegypti larvae. Copyright © 2016 John Wiley &amp; Sons, Ltd.

ПРИМЕНЕНИЕ ПИКРИНОВОЙ КИСЛОТЫ ДЛЯ КОЛИЧЕСТВЕННОГО АНАЛИЗА ХАМАЗУЛЕНА В ЦВЕТКАХ MATRICARIA CHAMOMILLA

In this paper the standardization of raw of Matricaria chamomilla with application of picric acid is proposed. The technique of quantitative determination of chamazulene content in Matricaria chamomilla flowers with spectrophotometric method is developed. In the study of the absorption spectrum of an alcoholic solution of essential oil of Matricaria chamomilla after treatment by picric acid revealed that its maximum occurs at 657 nm and coincides with that of picrate chamazulene. The point at 657 nm is chosen as analytical wavelengths.Experiments are conducted of "added-found" method to assess the accuracy of the developed technique and found that the relative error no more than 3,5%. The relative error of determination in series of successive measurements is no more than 2,5%, and in series of parallel measurements – 5,5%. Metrological analysis showed that the method is accurate and reproducible. The quantitative determination of chamazulene content defined in 6 batches of industrial raw materials using the developed technique. The total of chamazulene content in Matricaria chamomilla flowers is between 8,49–20,05 mg%. The chamazulene content in Matricaria chamomilla flowers in terms of absolutely dry raw material should be at least 8 mg%.

Changes in nutritional value and application of thyme (Thymus vulgaris) essential oil on microbial and organoleptic markers of Persian clover (Trifolium resupinatum) sprouts

In this study, effect of the 4-day germination on the chemical composition, antioxidant activity and fatty acids profile of Persian clover seeds (Trifolium resupinatum) was investigated. In addition, clover sprouts was immersed in 0.5, 1 and 2% thyme (Thymus vulgaris) essential oil (EO) solutions, packaged in polyethylene containers and stored at refrigerated temperature (4 °C) for 10 days. As seedling progressed, the moisture, protein, crude fibre and ash content increased, whilst fat and carbohydrate reduced. After 4-day germination, ascorbic acid, total phenolic content and antioxidant activity indicated 1.3, 2 and 1.5 fold increase, respectively. Sprouting caused significantly (p < 0.05) increase in measured organic acids and minerals (except K). Moreover, oleic acid showed an increase of 3.4% compared to raw seed. In respect to microbial quality, the treated clover sprout demonstrated a significant reduction in the population of total aerobic bacteria, coliforms, molds and yeast count followed by increasing EO concentration compared to the control. Total aerobic bacteria, total coliforms, molds and yeast count of clover sprouts with 2% thyme EO showed 3.2, 2.3 and 2.2 log10 unit reduction, just after treatment. During storage, the population of natural microflora increased significantly (p < 0.05) in all treatments.

Ex vivo vs. in vivo antibacterial activity of two antiseptics on oral biofilm.

Aim: To compare the immediate antibacterial effect of two application methods (passive immersion and active mouthwash) of two antiseptic solutions on the in situ oral biofilm.Material and Methods: A randomized observer-masked crossover study was conducted. Fifteen healthy volunteers wore a specific intraoral device for 48 h to form a biofilm in three glass disks. One of these disks was used as a baseline; another one was immersed in a solution of 0.2% Chlorhexidine (0.2% CHX), remaining the third in the device, placed in the oral cavity, during the 0.2% CHX mouthwash application. After a 2-weeks washout period, the protocol was repeated using a solution of Essential Oils (EO). Samples were analyzed for bacterial viability with the confocal laser scanning microscope after previous staining with LIVE/DEAD® BacLight™.Results: The EO showed a better antibacterial effect compared to the 0.2% CHX after the mouthwash application (% of bacterial viability = 1.16 ± 1.00% vs. 5.08 ± 5.79%, respectively), and was more effective in all layers (p < 0.05). In the immersion, both antiseptics were significantly less effective (% of bacterial viability = 26.93 ± 13.11%, EO vs. 15.17 ± 6.14%, 0.2% CHX); in the case of EO immersion, there were no significant changes in the bacterial viability of the deepest layer in comparison with the baseline.Conclusions: The method of application conditioned the antibacterial activity of the 0.2% CHX and EO solutions on the in situ oral biofilm. The in vivo active mouthwash was more effective than the ex vivo passive immersion in both antiseptic solutions. There was more penetration of the antiseptic inside the biofilm with an active mouthwash, especially with the EO. Trial registered in clinicaltrials.gov with the number NCT02267239. URL: https://clinicaltrials.gov/ct2/show/NCT02267239.

Antimicrobial Effects of Nisin, Essential Oil, and γ-Irradiation Treatments against High Load of Salmonella typhimurium on Mini-carrots.

This study aimed at using essential oil (EO) alone or combined EO with nisin and γ-irradiation to control Salmonella Typhimurium during the refrigerated storage of mini-carrots. Peeled mini-carrots were inoculated with S. Typhimurium at a final concentration of approximately 7 log CFU/g. Inoculated samples were coated by 5 different coating solutions: (i) nisin solution at final concentration of 10(3) IU/mL; (ii) mountain savory EO solution at 0.35%; (iii) carvacrol solution at 0.35%; (iv) mountain savory EO at 0.35% plus nisin solution of 10(3) IU/mL; or (v) carvacrol at 0.35% plus nisin solution of 10(3) IU/mL. Coated mini-carrots were then irradiated at 0.5 or 1.0 kGy and compared to an unirradiated control sample. Samples were kept at 4 °C and microbial analyses were conducted at days 1, 3, 6, and 9. The results showed that mini-carrots coated by carvacrol plus nisin solution or mountain savory EO plus nisin solution in combination with irradiation at 1.0 kGy completely eliminated S. Typhimurium to under the detection limit during the storage. Thus, the combined treatments using carvacrol plus nisin or mountain savory EO plus nisin coating solution and irradiation at 1.0 kGy could be used as an effective method for controlling S. Typhimurium in mini-carrots. This study shows the effect of using γ irradiation at low dose (1 kGy) to reduce significantly the growth of Salmonella typhimurium in mini-carrots. It also shows that combination of nisin, essential oils with γ irradiation have the best antibacterial effects against S. Typhimurium during the storage of mini-carrots. The results can be used for practical application in food industry in terms of food safety.

Chemical Composition of Essential Oil from Eucalyptus citriodora Leaves and Insecticidal Activity Against Myzus persicae and Frankliniella schultzei

Essential oils, volatile compounds obtained from plants, provide an alternative approach for controlling agricultural pests due to problems with conventional chemical products, such as insect resistance, environmental issues and human health concerns. This work aimed to extract essential oil from Eucalyptus citriodora to assess its chemical composition and insecticidal activity against the green peach aphid (Myzus persicae) and thrips (Frankliniella schultzei). The essential oil was obtained by hydrodistillation using the Clevenger apparatus and analyzed by gas chromatography with a flame ionization detector and mass spectrometry (GC-FID and GC-MS). A Potter tower was used to spray the insects with the essential oil solutions. The major components found in E. citriodora essential oil were citronellal (29.31 %), geraniol (27.63 %), β-citronellol (14.88 %) and δ-cadinene (6.32 %). Mortality of M. persicae and F. schultzei nymphs were 85.5 % and 34.8 %, respectively, using essential oil at 1 % (w v−1). Estimated values of LC50 (0.40 % w v−1) and LC90 (1.15% w v−1) for M. persicae evidences the potential use of E. citriodora essential oil in controlling this pest.

Antibacterial Effect of Various Essential Oils against Pathogens and Spoilage Microorganisms in Fresh Produce

This study was conducted to investigate the antibacterial effect of various essential oils (EOs) against pathogens and fresh produce spoilage bacteria using the agar disc diffusion method and 96-well dilution method. Moreover, the antibacterial effect of EOs to reduce the level of total mesophilic microorganisms in fresh leaf lettuce and radish sprouts was determined by dipping method. Furthermore, membrane damage of two pathogens before and after treatment with effective EOs was determined using the fluorescent dye propidium iodide (PI) staining method. The results showed that thyme-1, thyme-2 and tea tree-2 oils demonstrated the strongest antibacterial effect against 18 strains of pathogens, except for Pseudomonas aeruginosa, in the agar disc diffusion method. Thyme-1 oil showed the strongest antibacterial effect against spoilage bacteria. The dipping treatment results showed that oregano-2 oil was the most effective at maintaining reduced levels of total mesophilic microorganisms in fresh produce compared with control, although it affected the quality of both vegetables after treatment. The results showed that treatment with clove bud, cinnamon-2 and oregano-2 oils led to PI values significantly greater than that of untreated cell. Practical Applications Natural essential oil solutions can be used for the disinfection of fresh produce as an alternative to chlorine, and oregano oil could be used as an alternative to synthetic sanitizers, classically applied in fresh produce.

Effects of surface treatment with cinnamon oil and clove oil on mold resistance and physical properties of rubberwood particleboards

Surface treatment by dipping rubberwood particleboards in a solution of essential oil (cinnamon oil or clove oil to achieve concentrations of up to 0.63 %) to protect against molds (Aspergillus sp., and Trichothecium sp.) was investigated. GC–MS analysis was employed to investigate essential oil constituents left in the surface layer of particleboards for up to 12 weeks after the treatment. Effect of the treatment on some important physical and mechanical properties of particleboards was also examined. Dip treatment in cinnamon oil and clove oil at a concentration of 0.63 % was capable of providing complete protection for at least 8 and 5 weeks, respectively, against growth of the test molds on particleboards at 25 °C and 100 % RH. Treatment had no significant effect on physical and mechanical properties of the particleboards. Contents of cinnamaldehyde and eugenol in the particleboards, the main antifungal agents in cinnamon oil and clove oil, respectively, largely declined within the first 4 weeks of incubation. Reducing losses of these antifungal agents could further prolong antifungal performance of the particleboards.

English

The interest of researchers in various areas has resulted in the investigation of different biofilm systems using a wide range of techniques. Biofilms are microbial communities consisting of mono or multi-species sessile cells, embedded in a matrix of extracellular polymers (exopolysaccharides-EPS) adhering to surfaces. In the food industry, the existence of biofilms is quite problematic, being responsible for the economic loss and contamination of food. Consequently, research involving the characterization of the ability of microbial biofilm formation is relevant for the subsequent studies using sanitizing and antibiotic agents for prevention or remediation of surfaces with already formed biofilms. This multidisciplinary study led to the description regarding the effect of antimicrobial solutions of essential oils of Syzygium aromaticum and Thymus vulgaris and their combination on biofilm formed by Staphylococcus aureus ATCC 25923 on AISI 304 stainless steel and polypropylene surfaces. All sanitizing solutions showed antibacterial potential, being effective in reducing bacterial biofilms on these surfaces. The solution containing the combination of essential oils was the most efficient by reducing 7.38 and 6.58 Log CFU.cm-2 of cells adhering on the surfaces of AISI 304 stainless steel and polypropylene, respectively, after 5 min of contact. Essential oils of S. aromaticum and T. vulgaris, alone or in combination, are new alternatives for disinfection of industrial stainless steel and polypropylene surfaces contaminated by S. aureus. Key words: Antimicrobial effect, microorganism, Syzygium aromaticum, Thymus vulgaris.

English

Attachment of bacteria to food processing equipment surfaces can lead to product contamination, spoilage, and surface destruction. Most of bacteria in their habitat live in communities of different degrees of complexity, in general composing biofilms. Once installed the biofilm, the resistance of these sessile organisms increases, making it difficult thus the action of sanitizers. This multidisciplinary study led to the description regarding the effect of antimicrobial solutions of essential oils of Syzygium aromaticum and Thymus vulgaris and their combination on biofilm formed by Staphylococcus aureus ATCC 25923 on AISI 304 stainless steel and polypropylene surfaces and evaluate the possible effects of these oils on Staphylococcus aureus cells after treatment with sanitizing solutions based on these natural compounds. All sanitizing solutions showed antibacterial potential, being effective in reducing bacterial biofilms on these surfaces. The solution containing the combination of essential oils was the most efficient by reducing 7.38 and 6.58 Log CFU.cm-2 of cells adhered on the surfaces of AISI 304 stainless steel and polypropylene, respectively, after 5 min of contact. Key words: Antimicrobial effect, microorganism, Syzygium aromaticum, Thymus vulgaris.

Anti-bacterial Activities of Essential Oil Obtained from Hydrodistillation of Artemisia princeps var. orientalis

This experimental study reported the antibacterial activities of the essential oil obtained from Artemisia princeps var. orientalis from Kimpo, Gyunggi-do province (Korea). The yield of essential oil, collected by hydrodistillation, was0.02% w/w. The 1% solution of essential oil showed anti-bacteria...

Qualitative and quantitative biological studies of three chemotypes of basil essential oils grown in Malaysia against E.coli, S.aureus and P. aeruginosa

Different accessions of basil introduced to Malaysia as seeds and cultivated at University Malaysia Pahang (UMP) farm and their essential oils extracted by steam distillation. Three chemotypes of these essential oils with estragole, linalool and methyl cinnamate as dominant compounds were evaluated against three facultative anaerobic bacteria obtained from the National Pharmaceutical Control Bureau, Ministry of Health Malaysia: namely, Escherichia coli, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) by qualitative and quantitative method. Different concentration ranged from 10 μL/mL to 1000 μL/mL of basil essential oil solutions prepared and tested against bacterial strain using agar well diffusion and quantitative methods. Minimum inhibitory concentration (MIC) was performed using broth microdilution plate. Eight different concentrations of serial two-fold dilutions ranged between 250 μL/mL and 1.95 μL/mL performed using Magellan software of Tecan Infinite Series M200 Pro microplate reader. In the result of agar well diffusion test, the zone of inhibition increased as the concentration of essential oil increased. The MIC was 7.81 μL/mL for all chemotype of the oils against E.coli and S.aureus while 15.63 μL/mL for P.aeruginosa of linalool-rich chemotype and 31.25 μL/mL for estragole and methyl cinnamate-rich chemotypes. The results obtained in this study were considered encouraging the potential of basil essential oil for medicinal uses as antibiotics and hygienic purposes as antibacterial products.