Abstract

<span class="fontstyle0">This research focused on the study of the capability of cinnamon essential oil as antibacterial<br />agent, when it is made as a microemulsion solution. The study occupied further research on<br />specific type, concentration, and amount of emulsifier needed to make the cinnamon essential<br />oil miscible in water without causing destruction in its antibacterial activity. Four different<br />emulsifiers were tested, namely Tween 20, Tween 80, soya lechitin, and carboxymethyl cellulose<br />(CMC) to stabilize the essential oil in water. The emulsifiers were used at 2% w/w, while the<br />essential oil concentrations were varied at 0.5%, 1%, and 1.5%. In this study, the antibacterial<br />activity of the microemulsion solution of cinnamon essential oil was tested against two types of<br />pathogenic bacteria commonly found in foods, namely Staphylococcus aureus and Escherichia<br />coli. Among the four types of emulsifiers, which are used, CMC showed the best results as an<br />emulsifier. The results of this study indicated that microemulsion solution has antibacterial<br />activity best is a combination between the CMC and the essential oil of cinnamon</span>

Highlights

  • Saat ini masyarakat tampaknya mengalami tren "konsumerisme hijau", yang menginginkan bahan tambahan makanan yang mengandung sedikit bahan sintetis dan produk dengan dampak yang lebih kecil terhadap lingkungan

  • The results of this study indicated that microemulsion solution has antibacterial activity best is a combination between the Carboxymethyl Cellulose (CMC) and the essential oil of cinnamon

  • A systems biology approach sheds new light on Escherichia coli acid resistance

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Summary

Metode Pengujian Sifat Antibakteri Dalam Fase Cair dengan Desain Mikroemulsi

Sifat antibakteri dari minyak atsiri kayu manis dalam fase cair diteliti dengan menggunakan metode Disc Diffusion. Tipe media kultur yang dipergunakan Mueller – Hinton Agar. Ditempatkan 4 kertas cakram di permukaan Mueller – Hinton Agar. 3 kertas cakram mengandung ekstrak dari emulsifier dengan beberapa konsentrasi dan 1 kertas cakram mengandung Dimethylsulfoxide (DMSO) sebagai kontrol negatif. Berdasarkan literatur Performance Standards for Antimicrobial Susceptibility Testing (2014), setelah 16 – 18 jam inkubasi, setiap petri dish diamati. Apabila petri dish sudah diinokulasi, zona inhibitor akan terbentuk di sekitar kertas cakram. Selanjutnya diameter zona inhibitor diukur dengan menggunakan Vernier Caliper

Penelitian Pendahuluan
Penelitian Utama
Standards for Antimicrobial Susceptibility
Larutan Emulsi
DAFTAR PUSTAKA
Full Text
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