Mast cell tumors are cutaneous neoplasms in dogs, characterized by tumors that appear as solitary nodules in the skin or subcutaneous tissue, varying in appearance from raised, erythematous masses to nodular eruptions or diffuse swellings. This report describes a case of infiltrative subcutaneous mast cell tumor in a 7-year-old female dog weighing 22.3 kg. The animal presented with two nodules, one in the left femur-tibia-patellar region (22x30 mm) and another in the right inguinal region (20 mm), both mobile, non-adherent, and firm in consistency. Following complementary exams (hemogram, ultrasonography, and cytology), surgical excision of the nodules and sentinel lymph nodes was performed. After histopathological diagnosis, chemotherapy treatment with vinblastine and prednisolone was initiated. The dog responded positively to treatment, without adverse reactions, and showed no recurrence or metastases in the subsequent four months of follow-up.