Erigeron Breviscapus Research Articles

Simultaneous determination of active ingredients in Erigeron breviscapus (Vant.) Hand-Mazz. by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis (CE) with electrochemical detection (ED) method was developed for the determination of the pharmacologically active ingredients in Erigeron breviscapus (Vant.) Hand-Mazz. and its extract phytopharmaceuticals in this work. Under the optimum conditions, nine analytes, baicalein, naringenin, scopoletin, kaempferol, apigenin, scutellarin, luteolin, caffeic acid and protocatechuic acid were separated within 24 min in a borax buffer (pH 8.7). Notably, excellent linearity was obtained over two orders of magnitude with detection limits ( S/ N = 3) ranged from 1.0 × 10 −7 g/mL to 5.6 × 10 −7 g/mL for all nine analytes. This method was successfully used in the analysis of E. breviscapus (Vant.) Hand-Mazz. and its phytopharmaceuticals with a relatively simple extraction procedure, and the assay results were satisfactory.

A Study of the Cardioprotective Effect of Breviscapine During Hypoxia of Cardiomyocytesin vitroand During Myocardial Infarctionin vivo

Breviscapine is a flavonoid extracted from Erigeron breviscapus. Hand.-Mazz, and it has been reported that breviscapine can activate K+ channels and block Ca2+ channels. In this paper, we studied the cardioprotective effects of breviscapine on electrocardiogram (ECG) changes (ST-segment elevation), infarction size in dog heart subjected to myocardial infarction caused by left coronary artery ligation and lactate dehydrogenase (LDH) leakage, changes of intracellular free Ca2+ levels, apoptosis and necrosis in cultured neonatal rat cardiomyocytes subjected to hypoxia. Additionally, the effect of breviscapine on myocardial oxygen consumption was detected in dog myocardium in vitro. The results showed that breviscapine treatment (1 mg/kg, 2 mg/kg and 4 mg/kg) significantly reduced ST-segment elevation and infarction size in hearts subjected to myocardial infarction, that breviscapine treatment (14.29 microg/mL, 28.57 microg/mL and 57.14 microg/mL) significantly decreased oxygen consumption in myocardium, and that breviscapine treatment (5 microg/mL, 10 microg/mL and 20 microg/mL) significantly reduced LDH leakage, intracellular free Ca2+ levels, apoptosis and necrosis in cardiomyocytes subjected to hypoxia. In conclusion, the present study indicates that breviscapine is in favor of myocardial protection.

Quality evaluation of fingerprints of herbal medicine with chromatographic data

In this study, local least squares (LLS) and principal component analysis (PCA) were applied to deal with the disturbances in a data set of chromatographic fingerprints after necessary data transformations. It has been demonstrated that PCA with standard normal variate (SNV) transformation of data led to meaningful classification of 33 different Erigeron breviscapus herbal samples. The result was also corroborated by variance squares discriminant method. The quality of herbal objects was further evaluated, and the causes of this fact have been explained from a chemical point of view. At the same time, it implied an idea for qualitative evaluation of the herbal objects with a common class pattern of chromatographic fingerprints.

Antibacterial and antifungal activity of Erigeron breviscapus

The antibacterial and antifungal properties of the ethanol extract of Erigeron breviscapus whole plant was evaluated. The extract showed a moderate antibacterial activity and a high antifungal activity.

A New Sesquiterpene Glucoside from Erigeron Breviscapus

A new sesquiterpene glucoside, erigeside E, was isolated from the ethanol extracts of the whole plants of Erigeron breviscapus (Vant.) Hand-Mazz. Its structure was elucidated on the basis of spectral analysis.

The effect of Erigeron breviscapus on proliferation of pulmonary artery smooth muscle cells in hypoxic porcines.

In order to study the effect of Erigeron Breviscapus (EB) on proliferation of pulmonary artery smooth muscle cells (PASMC) in hypoxic porcines, immunohistochemical and MTT methods were employed to measure the proliferation of PASMC. It was found that the proliferation of PASMC in porcines was obvious, and the expression of proliferating cell nuclear antigen (PCNA) was significantly high within 48 h after exposure to hypoxia. The EB could inhibit the proliferation and the expression of PCNA in PASMC under hypoxia, but it had no effect on the proliferation and expression of PCNA in PASMC under normal condition. The EB could inhibit the proliferation and the expression of PCNA in PASMC induced by phorbol 12-myristate 13-acetate (PMA), an agonist of PKC in normal and hypoxic conditions. It was concluded that the hypoxia could enhance the proliferation and expression of PCNA in PASMC. The EB can inhibit the proliferation and expression of PCNA in PASMC under hypoxia through PKC-signal way. The EB may be used in treating the pulmonary hypertension by inhibiting the proliferation of PASMC and the pulmonary vascular remodeling.

Identification and determination of glucuronides and their aglycones in Erigeron breviscapus by liquid chromatography–tandem mass spectrometry

A novel, speedy and reliable LC–MS–MS method for the search, identification and quantitation of O-glycosides and their aglycones from plant resources was established by analyzing the extract of Erigeron breviscapus. The extract was directly infused to a triple–quadrupole MS–MS and major glucuronides in the extract were screened out with high confidence by a neutral loss scan for the loss of a gluconic acid. The identity of these glucuronides and their aglycones was further confirmed with LC–MS–MS. In addition to scutellarin, apigenin 7- O-glucuronide, quercetin-3- O-glucuronide and their aglycones that were previously reported by others, we also confirmed by LC–MS–MS that remarkable amount of baicalin, an isomer of apigenin 7- O-glucuronide, presented in the extract of this plant, which had not been reported before. A satisfying quantitation of three glucuronides was also made by LC–MS–MS.

Determination of scutellarin in Erigeron breviscapus extract by liquid chromatography–tandem mass spectrometry

A quantitative assay for scutellarin by LC–MS–MS (negative ion mode) was developed. The scutellarin was extracted from dry Erigeron breviscapus. Significant ion suppression was observed, which could be eliminated by increasing the turboionspray interface temperature to 350°C and by 1000-fold dilution of the extract with solvent. The calibration curve of scutellarin showed excellent linearity over a wide concentration range (0.01–100 μg/ml) ( r=0.998), and the limit of detection was 15 pg/ml using a 10-μl injection volume. The analysis time was 4 min/sample.

Two New Caffeoyl Conjugation from Erigeron Breviscapus

Two new constituents with a novel basic skeleton were isolated from Erigeron breviscapus. On the basis of chemical and spectroscopic evidences, the structures of the new compounds were elucidated as 1R,3R-dihydroxy-4S,5R-dicaffeoyloxy cyclohexane carboxylic acid methyl ester (V), 1,4-dihydroxy-3R,5R-dicaffeoyloxy cyclohexane carboxylic acid methyl ester (VI).

Erigeron breviscapus prevents defective endothelium-dependent relaxation in diabetic rat aorta

We examined the endothelium-dependent relaxation response to acetylcholine (Ach) in streptozotocin-induced diabetic rat aorta at the stages of 2- and 6-wks' duration in vitro, and compared with another two groups which were treated with dietary supplement of 0.1% Aminoquanidine (AG) and 0.5% Erigeron breviscapus(EB) from 1-week of diabetes induction. At the stage of 2-wks' duration of diabetes, relaxation responses to lower concentrations of Ach in 0.3uM phenylepherine-precontracted aortas were diminished significantly (P < 0.05) compared with age-matched control, but the maximal relaxation of Ach remained unchanged. At the stage of 6-wks' duration, diabetes caused an approximately 60% (P < 0.001) deficit in maximum relaxation, and this was significantly (P < 0.001) prevented in AG and EB treated groups. There was an approximately 40% enhancement in the maximum contractile response to phenylepherine with diabetes (P < 0.05), which was unaffected significantly by AG and EB treatments. The data suggest that the defective endothelium-dependent relaxation in diabetic rat aorta occurred as early as 2-wks' duration of diabetes, and the treatments of AG and EB could protect vascular endothelium although the deficits in vascular smooth muscle contractile responses were not protected.

A sesquiterpene and other constituents from Erigeron breviscapus

Three new Constituent erigesides, A-C, as well as two known compounds, betulabuside A and erigeroside, have been isolated from Erigeron breviscapus. The structures of the new compounds were established on the basis of spectral analysis.