The Biosphere 2 coral reef biome is a large, fully enclosed, self-sustaining mesocosm. Water is moved throughout the mesocosm by waves. Inorganic and organic nutrients were monitored weekly from 1995 to 2000. Eight nutrient-uptake experiments were conducted to measure uptake-rate constants (S, m s–1) for NH4, PO4, and NO3. Nutrient concentrations were low, except for DON, and typical of coral-reef ecosystems (means: NH4=0.63, NO3=0.62, PO4=0.05, SiO3=9.5, DON=41.2, DOP=0.26 mmol m–3). Nutrient uptake-rate constants varied in the range 54–126×10–6 m s–1 (4.6–11 m day–1) and correlated with water velocity. These rates, however, are 2–3-fold higher than rates for equivalent water velocities in steady, non-wave flows. Nutrients are recycled within the biome at rates sufficient to support gross production and, even in this recycling system, nutrient-uptake rates are mass transfer-limited.
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