Equilibration Volume Research Articles

High‐temperature, low‐pressure metamorphism and development of prograde symplectites, Marble Hall Fragment, Bushveld Complex (South Africa)

AbstractMetapelitic rocks from the Marble Hall Fragment, enclosed in the granites of the magmatic Bushveld Complex, record a two‐stage, low‐pressure, high‐temperature metamorphism. An early paragenesis containing chiastolitic andalusite, cordierite, biotite and quartz ± garnet crystallized in most rocks and equilibrated at 550–600 °C, 0.2 GPa. It was transformed during the second, peak event into various parageneses that commonly coexist within a single thin section. These include garnet–cordierite–biotite–K‐feldspar–quartz, sillimanite–cordierite–K‐feldspar–quartz and spectacular quartz‐undersaturated cordierite–spinel symplectites replacing the chiastolite porphyroblasts.Based on a detailed phase diagram analysis, we argue that these parageneses result from rapid heating at an approximately constant pressure to temperatures of more than about 720 °C. At these temperatures, the internally buffered activity of water was reduced by incipient water‐saturated partial melting, while only minor quantities of melt were produced. Subsequent dry conditions inhibited large‐scale equilibration and, together with local inhomogeneities in mineral distribution, led to the development of contrasting parageneses and symplectite textures. No signs of widespread fluid‐absent melting of biotite were found, and so the temperature probably did not exceed 760 °C. The peak metamorphic event is attributed to the emplacement of the hot Nebo granite, whereas the early metamorphism was probably caused by the intrusion of one of the phases of the Rustenburg Layered Suite.We infer the conditions of development of the cordierite–spinel intergrowths and we show that, although symplectites are commonly associated with retrograde processes (cooling and/or decompression), they can record a prograde metamorphic evolution. Furthermore, our contribution emphasizes the importance of the concept of reduced equilibration volume for the understanding and interpretation of some particular textures and parageneses in common rocks.

Gradient elution with normal phases on silica : A comparison between high-performance liquid and supercritical fluid chromatography

Gradient elution on silica in supercritical fluid chromatography ( SFC) and high-performance liquid chromatography ( HPLC) was compared. The instrumental set-up for the measurement of the equilibration volumes in SFC and HPLC is described. It was demonstrated by k′ measurements that 300–1000 column volumes in normal-phase HPLC are needed in order to achieve constant chromatographic conditions after a change of eluent. For this reason normal-phase gradient elution is an impracticable and irreproducible HPLC method for routine application. On the other hand, pressure gradient elution with supercritical carbon dioxide on silica showed very small equilibration volumes ( ca. 2 column volumes). Thermodynamic equilibration in the column is achieved instantly. The modifier change in SFC needs only 10–30 column volumes. Here the equilibration volume depends strongly on the density and diffusivities in the supercritical fluid. Consequently, the use of pressure and modifier gradients in SFC has great advantages over the use of gradient elution in normal-phase HPLC.

Influx and cellular degradation of low density lipoproteins in rabbit aorta determined in an in vitro perfusion system.

The accumulation of 125I-low density lipoprotein (LDL) into normal and atherosclerotic arterial tissue and cellular uptake in arterial cells were studied in an in vitro perfusion system for rabbit aorta. The accumulation of 125I-LDL in normal tissue could be fitted to an inverse exponential function with an initial influx rate of 1.39 nl/mg wet weight/hour and an equilibration volume of about 2% of the tissue volume. The influx rate into atherosclerotic plaques was about 10 times faster and the equilibration volume, 50 times higher. In atherosclerotic tissue there was a steep concentration gradient between the plaque and the underlying media. The accumulation of 125I-LDL in the media under plaque and in normal tissue adjacent to plaques was similar to that seen in normal tissue. For studies of cellular uptake of LDL a trace label, 125I-tyramine-cellobiose (TC), was used. Normal or atherosclerotic rabbit aorta was perfused in vitro with medium containing 125I-TC-LDL. After perfusion the tissue was digested and the cells were isolated by density gradient centrifugation. Two main cell fractions with characteristics of smooth muscle cells and foam cells, respectively, were obtained. A 70-fold higher uptake was seen in the foam cells. In conclusion, these studies suggest a higher influx rate into atherosclerotic plaques, as well as a high LDL concentration in the plaque, compared with normal tissue or underlying media. We suggest that most of the cellular uptake of LDL in the arterial wall is caused by the foam cells.

Plasma water and 51Cr EDTA equilibration volumes of different tissues in the rat.

The EDTA space and the plasma water volume of the whole animal and selected tissues were investigated with constant EDTA infusions of different duration in the rat. The calculated EDTA space increased with the duration of the infusion both in the whole animal and the tissues sampled. The plasma water volume remained constant, and therefore the calculated increase of the EDTA space suggests a slow intracellular accumulation of the tracer. As intracellular accumulation of EDTA increases with experimental time, it is necessary to use as short an experimental interval as possible to reduce this error when determining extracellular fluid volumes.