Epithelium Thickness Of Seminiferous Tubules Research Articles

Effect of Psidium guajava Juice on The Seminiferous Tubules Diameter and Epithelium Thickness in Rattus norvegicus Exposed by Lead Acetate

Lead is one of humans and animals' most common and hazardous heavy metals. This study aimed to investigate the effect of white guava (Psidium guajava) fruit juice on the seminiferous tubule diameter and epithelium thickness in rats (Rattus norvegicus) exposed to lead acetate. The research design was a completely randomized design (CRD). A total of 25 male rats with an average weight of 200 grams were used for the study, divided into 5 treatment groups, each consisting of 5 rats. The treatments were as follows: the control group (C) was given distilled water orally; the T0 group was induced with lead acetate (50 mg/Kg BW) orally; the T1, T2, T3 groups were induced with lead acetate (50 mg/Kg BW) and then given 25%, 50%, and 100% concentration of white guava fruit juice, respectively. All the treatments were conducted for 14 days. The histopathology slides of the testis were made with HE staining, and the seminiferous tubule diameter and epithelium thickness were measured. The data were analyzed using One Way ANOVA and Duncan test (p<0.05). The results showed that the control group (C) which was given distilled water only had a seminiferous tubule diameter and epithelium thickness of 336.24±23.32 µm and 66.46±4.39 µm, respectively. The T0 group which was induced with lead acetate only showed a reduction in the diameter and epithelium thickness of seminiferous tubules (243.38±49.35 µm and 44.08±14.45 µm). The members of the T1, T2 and T3 groups showed positive effects on the diameter (323.49±22.82 µm; 314.41±13.04 µm; 325.04±16.88 µm, respectively) and epithelium thickness (56.36±3.36 µm; 60.50±3.81 µm; 66.744±9.50 µm, respectively). There was no significant difference reported between each group. The administration of guava juice to rats induced with lead acetate can positively affect the diameter and epithelium thickness of seminiferous tubules.

Virgin coconut oil protected the diameter and the epithelium thickness of seminiferous tubules of mice (Mus musculus) from oral ethanol induction

This study investigated the preventive effect of virgin coconut oil (VCO) on the epithelium thickness and the diameter of the seminiferous tubules induced by ethanol in mice (Mus musculus). This study used 20 male mice as the experimental animal which were divided into five groups with four mice in each group. Negative control (C-) was given 2% Tween and aquadest, while positive control (C+) was given 2% Tween and 33% ethanol. T1, T2, and T3 were respectively given 0.09, 0.19, and 0.37 mL/kg bw VCO and 33% ethanol (0.2 mL/kg bw). VCO was given orally for 39 days, and ethanol was given orally seven days later for 32 days. Ethanol was administered two hours after the VCO administration. Analysis of variance followed by Tukey’s range test on the epithelium thickness and the diameter of the seminiferous tubules showed significant differences (p <0.05) of C+ group from the other groups. Whereas, there was no significant difference (p >0.05) was found among C-, T1, T2 and T3 group. The result concluded that VCO could protect the testis of mice from the damage caused by ethanol.

AFRICAN BITTER LEAF EXTRACT EFFECT ON EPITHELIUM THICKNESS OF SEMINIFEROUS TUBULES IN WISTAR RATS WITH DIABETES MELLITUS

Introduction: Diabetes mellitus (DM) is a metabolic disease that can cause complications in the form of damage to male reproductive organs, such as decreased sperm motility and cell count, impaired spermatogenesis, and erectile dysfunction. A measurement that can be done to detect male reproductive disorders is to measure the thickness of the seminiferous tubule epithelium. Herbal medicines can be used to support treating diabetes. Studies have claimed that African bitter leaf helps in treating DM and its complications. Purpose: This study aims to determine the effect of African bitter leaf extract on epithelium thickness of seminiferous tubules in rats with DM. Method: True Experimental with Post-Test Only Control Group Design using Rattus norvegicus. Rats were divided into five groups, with six rats in each group. Rats were administered with a single dose of alloxan 150 mg/kg BW/IP. Group K is diabetic rats given glibenclamide 0,63 mg/kg BW. Group P0 are diabetic rats given CMC 0,1% 0,7 ml. Group P1 are diabetic rats given African bitter leaf extract 100 mg/kg BW. Group P2 are diabetic rats given African bitter leaf extract 200 mg/kg BW. Group P3 are diabetic rats given African bitter leaf extract 400 mg/kg BW. Treatments were given with oral gavage for 14 days. Rats were dissected, and their testis was made as histological slides. Results are statistically tested with ANOVA. Results: The average epithelium thickness from group K are 79,61 μm, group P0 are 59,96 μm, group P1 are 71,87 μm, group P2 are 67,31 μm, group P3 are 63,95 μm showed significant result (p=0,000). Conclusions: African bitter leaf extract affects epithelium thickness of seminiferous tubules, with group K, shows the best result, followed by group P1.

PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p<0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p<0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.

Effect of Kebar Grass (Biophytum petersianum) Extract on the Seminiferous Tubules in Male Mice (Mus musculus) Treated With 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

The aim of this research was to analyze the effect of kebar grass on the seminiferous tubules diameter and epithelium thickness of mice exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD). Twenty five BALB/C mice were used with five treatments (4 mice each group): Negative Control (C-), Positive Control (C+) with TCDD 7μg/kgBW IP, and treatment groups that treated with TCDD 7μg/kgBW IP and Kebar Grass orally for 53 days were presented T1 0,045mg/gBW/day, T2 0,080mg/gBW/day, and T3 0,135mg/gBW/day. Results showed that TCDD decreased the diameter and epithelium thickness of seminiferous tubules, while kebar grass extract was proven to maintain diameters but could not maintain epithelium thickness of of the seminiferous tubules Keywords : Kebar grass, Seminiferous tubule, Tetrachlorodibenzo-p-dioxin (TCDD), Mice

Dietary Guanidinoacetic acid modulates testicular histology and expression of c-Kit and STRA8 genes in roosters

Decline in semen quality is considered as a major contributing factor in age-related subfertility of broiler breeder flocks. This study was aimed to investigate the effect of dietary supplementation of Guanidinoacetic acid (GAA), as an alternative energy source along with antioxidant potential, on testicular histology and relative gene expression of some spermatogonial markers (c-Kit and STRA8) in aged roosters. Sixteen 24-week-old male broiler breeders were randomly allocated into four groups and fed a basal diet supplemented with increasing levels of GAA including 0 (GAA-0), 600 (GAA-600), 1200 (GAA-1200) or 1800 (GAA-1800) mg/kg diet/day for 26 successive weeks. At the end of the experiment, all the birds were killed and two ipsilateral testicle samples were taken to either quantify relative gene expression or do histology. Except for seminiferous tubules’ diameter, testicular weight, and the number of blood vessels, dietary supplementation of GGA improved the epithelium thickness of seminiferous tubules, the number of spermatogonia and Leydig cells and the relative gene expression of c-Kit and STRA8 (P < 0.01). Increasing levels of GAA cubically affected (P < 0.01) the diameter of seminiferous tubules and their epithelium thickness as well as the number of spermatogonia. However, number of Leydig cells and relative expression of c-Kit were linearly, and relative expression of STRA8 was quadratically (P < 0.01) enhanced in response to graded levels of GAA supplementation. Taking all parameters into account, daily supplementation of 1300–1450 mg of GAA/kg diet was estimated as an optimum dosage maximizing the evaluated traits.

Hypothyroidism effects on the morpho-histometrical features of testes in adult rats

Background: Up until now, the effect of thyroid hormones on the male reproductive organ, after puberty, is not well understood. OBJECTIVES: In the present study, the morpho-histometrical effects of thyroid hormone deficiency on the adult-male rat reproductive organs were investigated. MATHODS: Thirty adult male rats were randomly divided into three groups (n=10): control, hypothyroid, and hypothyroid which receives Levothyroxine. After 4-months we examined the number of spermatogonia cells, primary spermatocytes, and Sertoli cells, as well as the seminiferous tubules epithelium thickness, area of seminiferous tubules, and the diameter and thickness of testis artery walls. RESULTS: No significant difference was observed between the hypothyroid and control rats in terms of the area of seminiferous tubules, the number of spermatogonia cells, primary spermatocytes, and Sertoli cells. Moreover, the epithelium thickness of seminiferous tubules and the diameter and thickness of artery walls were significantly decreased in hypothyroid group compared with the control rats. CONCLUSIONS: These observations are consistent with the hypothesis that hypothyroidism can affect the male fertility and declare that it may influence male reproduction by reducing the testis blood flow.

Influence of immunocastration vaccine administration interval on serum androgen concentrations and testis activity in ram lambs

The objective of this study was to establish whether different intervals between primary and secondary immunocastration vaccinations had similar effects on the production of C-19 steroid hormones and testis tissue functionality. Forty Dohne Merino rams (5.5 months old) were allocated to the respective treatment groups, which included no vaccination (R), and four (IC4), three (IC3) or two (IC2) weeks between the primary and secondary vaccination. Administration of the secondary vaccination to all immunocastrated lambs occurred four weeks prior to slaughter. Blood samples were collected weekly for eight weeks, and serum C-19 steroid metabolites were determined with ultra-performance convergence chromatography tandem mass spectrometry. Testes were collected at slaughter to assess size and level of tissue activity, as indicated by the cut surface CIE Lab colour measurement, seminiferous tubule circumference and seminiferous tubule epithelium thickness. Serum cortisol concentrations fluctuated but immunocastration had no influence. Serum testosterone concentrations decreased in IC4, IC3 and IC2 lambs between primary and secondary vaccinations, but consistently low testosterone concentrations (<0.5 ng/mL) were only observed a week after the secondary vaccination. Immunocastration resulted in a decrease in testis weight, seminiferous tubule circumference and tubule epithelium thickness across all vaccination treatments, which indicates impaired spermatogenesis. However, the most pronounced decrease in seminiferous tubule epithelium depth was observed in the testes of IC3 and IC2 lambs. The CIE colour a* values measured on the cut testis surface differed between vaccination intervals, indicating potential differences in the extent of interrupted activity. Therefore, the immunocastration vaccination interval of lambs is flexible, while still being successful in suppressing testosterone secretion and interrupting functioning of the testes.

Short-term buserelin administration induces apoptosis and morphological changes in adult rat testes.

To investigate the effect of buserelin on gonadal structure and function in adult male rats. Twenty-four adult Wistar male rats were divided into three groups: two treated groups and controls. The first and second treated groups received 300 (low dose) and 500 (high dose) µg/kg buserelin, respectively, and the control group received normal saline. All groups were treated subcutaneously for five days. The seminiferous tubular epithelial thickness was significant decreased in the treated groups compared with those in the control. There was a significant increase in apoptotic cell death in high dose treated group compared with low dose treated and control groups. No significant difference in serum testosterone level was observed after one month in the three groups. Buserelin induces apoptotic cell death and decreased diameter and epithelium thickness of seminiferous tubules in the adult rat testes.

Long term evaluation of morphometric and ultrastructural changes of testes of alloxan-induced diabetic rats

To evaluate in a long term the morphometric and ultrastructural changes in seminiferous tubules (ST) of normal and diabetic rats, and to correlate any changes with animal age and diabetes duration. Sixty male Wistar rats, three months-old, were randomly divided into two groups: 30 non-diabetic controls (N) and 30 alloxan untreated diabetic (D). After one, six and 12 months of follow-up or diabetes induction rats were sacrificed and the testes examined. Morphometric measures of the ST were performed by digital imaging analysis. ST ultrastructure was analyzed by transmission electron microscopy. Sustained hyperglycemic state was observed in all diabetic rats throughout the study. Serum testosterone was also significantly decreased in these animals. The diameter, total area, epithelium area and epithelium thickness of ST were lower and tubular density was higher in diabetic animals. Diabetic rats also showed ultrastructural changes compromising the whole testis including germ-, Sertoli-, and Leydig cells, and also the mithocondria and cellular nuclei. Most frequent of these consisted of vacuolization and/or accumulation of lipid droplets and electron dense dark material in cell cytoplasm and/or in membranes, cellular degeneration, and apoptosis. Non-diabetic control rats also showed testicular lesions that resemble to the diabetic lesions, although much less severe and with later onset in life of these animals. Histopathological changes observed in testes of normal and diabetic rats are closely related to the animal age and/or duration of the hyperglycemic state, being progressively more severe in animals sacrificed belatedly. These changes may play an important role in male infertility observed in diabetic subjects.