PURPOSE: To study characteristics and associations of Gunn dots in a cohort of healthy children aged 11-12 years. METHODS: As part of the Copenhagen Child Cohort 2000 Study, red-free fundus photographs were taken on 761 healthy children aged 11-12 years. The photographs were centered on the optic disc. Gunn dots were annotated and counted within a disc-centered circular grid of 6 mm diameter. Data were analyzed in relation to age, sex, axial length, and retinal nerve fiber layer thickness. RESULTS: Gunn dots were found in 716 (94 %) of the 761 participants. The majority of dots were located both inferior and superior to the optic disc, situated within a distance of 3 mm from its center. The median number of Gunn dots was 64 (range 0 to 574) in right eyes and 68 (range 0 to 532) in left eyes. Having more than the median number of Gunn dots in the right eye was associated with older age (odds ratio (OR) 2.12 CI95 1.56 to 2.89, P<0.0001, adjusted for sex, axial length and retinal nerve fiber layer thickness and was less common among the children with a thinner retinal nerve fiber layer (OR 0.40, CI95 0.26 to 0.60, P<0.0001 comparing bottom and top quartiles). CONCLUSIONS: Gunn dots were visible in the majority of healthy children aged 11-12 years and large numbers of dots were associated with a thicker nerve fiber layer and with older age, within the limited age-range of one year. The clinical significance of Gunn dots is unknown. Their location at the vitreoretinal interface makes them a parameter of interest in the study of aging, epiretinal fibrosis, macular pucker and retinal detachment.