Enzymes Creatine Kinase Research Articles

Capture myopathy in wild birds: clinical information, a systematic review

Capture myopathy is a complication of handling in many species of mammals and wild birds. This pathology is described as a state of muscle tissue degradation that can render a bird unable to stand, walk or fly. This study is a systematic review of the literature on capture myopathy in wild birds with information searched between years 1980 to 2022. Taxonomic information, clinical signs, diagnostic methods, and treatments used in this pathology were compiled, summarized, and classified. A modified evaluation method was used for the methodological analysis of each of the articles, meeting inclusion and exclusion criteria, to have a desirable methodological quality, resulting in a total of 12 included studies. A higher prevalence of Capture myopathy was described in the families Scolopacidae, Gruidae and Anatidae. Frequent clinical signs associated with myopathy, were the inability to incorporate, ataxia, paresis, and paralysis. The diagnostic methods are based on clinical pathology, evaluating the enzymes creatine kinase and aspartate aminotransferase with their respective levels. Common macroscopic alterations were changes in the coloration of the muscle tissue and hemorrhagic areas due to rupture of myofibril and a frequent microscopic lesion was damage to muscle myofibers. The literature describes supportive treatments such as the early use of fluid therapy, analgesics, tranquilizers, vitamins, sodium bicarbonate, while physical therapy is beneficial to protect from injury and incorporate the bird to the environment as soon as possible, however, prevention is the most effective way to avoid the onset of this disease.

Aripiprazole-induced quasi-neuroleptic malignant syndrome: two case reports

BackgroundSignificant elevation of creatine kinase levels (above three digits) and leucocytosis in the absence of muscle rigidity, tremors, or autonomic dysfunction can pose a real challenge in the context of antipsychotic treatment as an early herald of neuroleptic malignant syndrome.Case presentationWe present here two cases of adult male patients of Black British heritage, ages 51 years and 28 years, respectively. Both received a diagnosis of schizoaffective disorder and presented with massive increase of creatine kinase blood level after aripiprazole depot administration, one with pernicious increase associated with silent neuroleptic malignant syndrome, and the second with asymptomatic benign enzyme elevation.ConclusionThough aripiprazole use is less likely to cause neuroleptic malignant syndrome, on rare occasions it can produce massive symptomatic or asymptomatic increase in serum creatine kinase enzyme levels, raising the need for close monitoring, especially at the initial doses of the drug.

Effects of dietary astaxanthin supplementation on growth performance, antioxidant status, immune response, and intestinal health of rainbow trout (Oncorhynchus mykiss)

A feeding trial was conducted to assess the impacts of dietary astaxanthin from wall-broken Haematococcus pluvialis (WBHPA) on the growth performance, antioxidant status, immune response, and intestinal health of rainbow trout (Oncorhynchus mykiss). Six experimental diets were formulated with various concentrations of WBHPA, ranging from 0 to 8.4 g/kg (containing 0 to 125 mg/kg astaxanthin). Each diet was fed to triplicate groups of rainbow trout (mean initial weight of 561 g) twice daily for 9 consecutive weeks. The survival rate and feed intake of fish exhibited no significant differences among the dietary groups (P > 0.05). Similarly, dietary inclusion of 25 to 100 mg/kg astaxanthin did not significantly affect the weight gain and daily growth coefficient (P > 0.05), but excessive inclusion of astaxanthin (125 mg/kg) slightly depressed these parameters (P < 0.05). Dietary inclusion of 25 to 50 mg/kg astaxanthin increased the activities of intestinal digestion and absorption enzymes (lipase, creatine kinase, and alkaline phosphatase), while the inclusion of 25 to 75 mg/kg astaxanthin improved the immune response of fish. Furthermore, regardless of inclusion level (25 to 125 mg/kg), dietary astaxanthin supplementation strengthened the intestinal mucosal barrier function and improved antioxidant activity, thereby promoting intestinal development. Conclusively, 25 to 75 mg/kg astaxanthin from WBHPA was recommended to be included in diets for rainbow trout.

Alterations in Alanine Transaminase, Aspartate Transaminase, Gamma-Glutamyl Transpeptidase, and Creatine Kinase in Acne Patients Undergoing Isotretinoin Treatment: A Retrospective Evaluation of Laboratory Tests.

Background Isotretinoin therapy is a commonly prescribed medication by dermatologists for the treatment of acne. Regular laboratory assessments are recommended throughout the treatment period to detect any potential complications. Objectives This study aims to present the alterations in laboratory parameters throughout the course of isotretinoin therapy and identify required diagnostic testing. Methods This study involved 136 patients undergoing isotretinoin treatment at doses of 0.3-0.5 mg/kg/day, with ages ranging from 18 to 41 years. A retrospective evaluation was conducted on biomarkers including aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), creatine kinase (CK), triglycerides, total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), hemoglobin, white blood cells, and thrombocytes. Levels of these parameters were analyzed prior to treatment and at the third month of treatment from the records of patients and the data were compared statistically. Moreover, the parameters of ALT, AST, CK, and GGT were graded objectively, and any alterations were noted in the patients. Results The levels of ALT, AST and GGT, along with triglycerides, total cholesterol, LDL-C, and thrombocyte levels showed significant elevation (p=0.001, p<0.001, p<0.001, p=0.001, p<0.001, p<0.001, and p=0.003, respectively). A significant decrease in HDL-C with hemoglobin was also noted (p=0.022, p=0.006, respectively). One patient (0.73%) exhibited grade 1 elevations in ALT, AST, and CK. One patient (0.73%) displayed grade 1 elevations in ALT and AST. One patient (0.73%) exhibited grade 1 elevations in AST and CK, while another patient (0.73%) had grade 1 elevationin AST and grade 3 elevationin CK. Furthermore, one patient (0.73%) had a grade 1 elevation exclusively in ALT, two patients (1.47%) had a grade 1 elevation exclusively in AST, and six patients (4.41%) exhibited a grade 1 elevation in CK only. No grade changes were observed in the GGT levels in the patients. Conclusion During isotretinoin treatment, changes in ALT and AST levels were more frequently associated with the muscle enzyme CK, while GGT levels remained unaffected. Therefore, GGT can be considered a reliable parameter for evaluating liver function in patients undergoing isotretinoin treatment.

A review of massage on physiological and psychological performance

This paper aims to succinctly summarize the existing body of literature concerning the effects of massage on sports and exercise performance, particularly focusing on motor skills, neurophysiological factors, and psychological factors. The review adheres to the PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-analysis) and encompasses a total of 76 articles. The findings suggest that, on the whole, massages do not exert a significant influence on motor skills, except for flexibility. Nevertheless, some studies propose that favorable changes in muscle force and muscular strength may be noticeable 48 hours after undergoing a massage. Regarding neurophysiological aspects, massages do not seem to impact factors such as clearance of blood lactate, circulation in the muscle, blood circulation, temperature in the muscle tissue, or activation of muscles. However, there is substantiated evidence supporting the idea that massages can alleviate pain and mitigate delayed-onset muscle soreness, potentially by reducing creatine kinase enzyme levels and through psychological processes. Additionally, the review underscores the psychological advantages of massage. It is documented that massage treatments lead to a reduction in feelings of depression, stress, anxiety, and perceived fatigue while simultaneously fostering enhancements in mood, relaxation, and opinion about recovery states. Massages may not have a direct impact on certain performance aspects, but they offer notable psychological benefits for sports, and exercise performance is questionable. They also play an indirect role as an important tool for promoting focus, relaxation, and recovery in athletes. Massages can aid athletes in staying mentally and physically prepared during competitions or training sessions.

The Role of Mitochondria in Statin-Induced Myopathy.

Statins represent the primary therapy for combatting hypercholesterolemia and reducing mortality from cardiovascular events. Despite their pleiotropic effects in lowering cholesterol synthesis, circulating cholesterol, as well as reducing the risk of other systemic diseases, statins have adverse events in a small, but significant, population of treated patients. The most prominent of these adverse effects is statin-induced myopathy, which lacks precise definition but is characterised by elevations in the muscle enzyme creatine kinase alongside musculoskeletal complaints, including pain, weakness and fatigue. The exact aetiology of statin-induced myopathy remains to be elucidated, although impaired mitochondrial function is thought to be an important underlying cause. This may result from or be the consequence of several factors including statin-induced inhibition of coenzyme Q10 (CoQ10) biosynthesis, impaired Ca2+ signalling and modified reactive oxygen species (ROS) generation. The purpose of this review article is to provide an update on the information available linking statin therapy with mitochondrial dysfunction and to outline any mechanistic insights, which may be beneficial in the future treatment of myopathic adverse events.

The effect of hibernation on the morphological structure and expression of creatine kinase in the intestine of Pelodiscus sinensis

The intestinal function of hibernating animals undergoes seasonal changes, during which they are in a fasting state, which also affects the morphology, structure, and function of intestine. The aim of this study was to analyse the histological differences in the intestinal structure, goblet cells, and mucosa of the initial (ISI), anterior (ASI), middle (MSI) and posterior (PSI) of small intestine of Pelodiscus sinensis during the whole hibernation process and to investigate the role of the key enzyme creatine kinase (CK) in regulating energy homeostasis during hibernation. The results of HE staining showed that the intestinal mucosa of the P. sinensis during hibernation presented detachment and atrophy of villus, and narrowing of gaps while the villous length of the ISI, ASI, MSI, and PSI were significantly decreased than those in normal stage. As hibernation progresses, the intestinal crypt of the ISI first increased and then decreased significantly. The AB-PAS staining results revealed that as hibernation progressed, the number of type Ⅰ goblet cells in the ISI significantly increased, while the number of type Ⅲ goblet cells significantly decreased. The immunohistochemical results revealed that CK-MB was highly expressed in the intestinal villus of P. sinensis. During hibernation, the expression of CK-MB in P. sinensis continued to increase in the ISI and decrease in the PSI. In summary, the results of this study suggested that hibernation process could alter the morphological structure of the ISI and ASI of P. sinensis, making them more vulnerable. Meanwhile, the ISI may protect the mucosal tissue during hibernation by increasing type Ⅲ goblet cells and decreasing type I goblet cells. During this process, the continuously increasing expression of CK in the ISI may participate in the adaptation of P. sinensis to intestinal hibernation by regulating energy metabolism.

On the significance of aspartate aminotransferase and creatine kinase in wild reptile health studies.

In reptile medicine, the enzymes aspartate aminotransferase (AST) and creatine kinase (CK) have been used in clinical diagnostics, where CK is considered an enzyme specific to muscle cell damage, while AST is a nonspecific enzyme that is mainly produced in the liver and muscle. When many native reptiles are sampled, it is evident that there are important differences between species and individuals belonging to the same species, making the AST and CK ranges very wide. The minimum and maximum values, variations and standard deviations were extracted for each enzyme from 17 wild reptile studies, revealing high variation and a wide range of variation for each species. AST and CK must be interpreted with caution in wild reptiles since there appears to be an important amount of individual and specific variation due to the muscular origin of these enzymes, and such variations tell us that there are considerable differences between individuals, physiological characteristics or sampling methods; thus, there is no apparent value derived from these kinds of studies on the utility of AST for evaluating liver damage, but the measurement of AST and CK can be useful for reptile health assessments or any manipulative study since they can eventually be used as indicators or potential biomarkers for restraint techniques or holding time.

Study the effectiveness of paraoxonase-1 and creatine kinase enzymes in acute myocardial infarction patients

Study the effectiveness of paraoxonase-1 and creatine kinase enzymes in acute myocardial infarction patients

Evaluation of pharmacological alternatives to reduce the pain and discomfort produced by electroejaculation in rams

AbstractElectroejaculation (EE) represents the main technique for semen collection from domestic and wild animals independently of libido. However, the technique is associated with intense involuntary muscle contractions, vocalization, ataxia and lying down, caused by the electric stimulation of the nerves in the caudal epigastric region. These clinical manifestations represent important indicators of discomfort. In this context, the objective of this study was to evaluate two protocols of local anaesthetic blockade and two anatomical access for pharmacological desensitization of the caudal epigastric innervation as alternatives to promote comfort and reduce stress associated with EE in rams. For the study, four clinically healthy Dorper rams were selected. All animals were subjected to a design consisting of five semen collection treatments (n = 3 collections per treatment): T1—control, conventional EE without local anaesthetic blockade; T2, EE with ventral blockade (VB) of epigastric innervation using lidocaine hydrochloride 2%; T3, EE with VB of epigastric innervation using a combination of lidocaine hydrochloride 2% and fentanyl citrate; T4, EE with blockade of epigastric innervation through the perineal access using lidocaine hydrochloride 2%; T5, EE with blockade of epigastric innervation through the perineal access using a combination of lidocaine hydrochloride and fentanyl citrate. Seminal samples resulting from EE were subjectively evaluated for sperm motility and concentration, vigour and volume. Additionally, blood serum samples were collected for quantification of cortisol and creatine kinase (CK) enzyme. Assessments of stress and discomfort were conducted by measuring blood pressure, heart rate (HR) and respiratory rate (RR), as well as observing involuntary muscle contractions, ataxia and animal vocalization. No variations in blood pressure, sperm motility, vigour, CK, and cortisol were observed among the treatments. Individual variations were observed for the occurrence of vocalization (p = .0066), but there were no differences between the groups. Anaesthetic blockades conducted using the combination of lidocaine and fentanyl resulted in a lower incidence of ataxia during EE (p &lt; .0001). It is concluded that the combination of fentanyl citrate and lidocaine hydrochloride results in less discomfort for animals undergoing EE, regardless of the anatomical access used for local anaesthetic blockades.

Relationship of CK-MB Levels with Troponin T in Patient with Coronary Heart Disease at Siloam Hospital Jambi

Background: Coronary heart disease (CHD) is one of the cardiovascular diseases which is the highest cause of death in the world. CHD occurs due to the build-up of cholesterol plaque which causes narrowing of the arteries. CHD is characterized by an increase in biomarkers, especially cardiac troponin and Creatine Kinase enzyme activity. The aim of this research is to determine the relationship between CK-MB and Troponin T in CHD patients.&#x0D; Method: The type of this research is descriptive study with a cross sectional approach. The population of this study was all patients who had been clinically diagnosed with CHD by doctors at Siloam Jambi Hospital according to their medical records. The sampling technique used was a total sample technique that met the inclusion and exclusion criteria. To analyze the relationship between variables, a parametric statistical test is used, namely the Pearson correlation test.&#x0D; Result: The results of the study show the average value of CK-MB activity = 46.12 IU/L and Troponin T = 359.53 ng/L. The Pearson correlation test analysis showed that there was a significant relationship between CK-MB and Troponin T (p &lt;0.00001), and the correlation coefficient (r) is 0.636.&#x0D; Conclusion: The activity of the CK-MB enzyme and the levels of Troponin T enzymes have a strong relationship in CHD patients at Siloam Hospital Jambi.

Stem cell biotherapy: A new remedy for Trichinella spiralis-induced inflammatory myopathy.

Trichinella spiralis (T. spiralis)-induced myopathy is an inflammatory myopathy that is difficult to treat unless the parasite is combated in its early intestinal phase before it reaches the muscles. This study aimed to evaluate the effect of local mesenchymal stem cell (MSC) therapy on T. spiralis-induced inflammatory myopathy in rats. Rats were divided into four groups: Group 1 (non-infected non-treated group); Group 2 (infected non-treated group); Group 3 (infected albendazole (ABZ)-treated group); and Group 4 (infected MSC-treated group). Their muscle status was assessed physiologically with the righting reflex and electromyography (EMG), parasitologically with the total muscle larval count, histopathologically with hematoxylin and eosin and Mallory's trichrome stains, as well as immunohistochemically for myogenin as a marker of muscle regeneration. Additionally, serum muscle enzymes creatine kinase (CK) and lactate dehydrogenase (LDH), as well as muscle matrix metalloproteinases MMP1 and MMP9, were assayed. Finally, the immunological response was assessed by measuring the levels of the muscle inflammatory cytokines tumor necrosis factor-alpha (TNF-α), interferon-gamma (INF-γ), and interleukin-4 (IL-4). Our findings revealed that MSC therapy markedly improved muscle EMG and righting reflex, as well as the histopathological appearance of the muscles, reduced inflammatory cellular infiltrates, and increased myogenin immunostaining. It also reduced serum CK and LDH levels, as well as muscle INF-γ, TNF-α, IL-4, MMP1, and MMP9 levels. However, it had no effect on the total muscle larval count. Accordingly, due to its anti-inflammatory properties and muscle-regenerative effect, MSC therapy could be a promising new remedy for T. spiralis-induced myopathy.

Болезнь Помпе у взрослых: клинические проявления, диагноз и лечение

Pompe disease is a rare inherited lisosomal storage disease characterized by accumulation of glycogen in muscular cells due to a deficient activity of acid a-glucosidase (GAA), an enzyme which degrades lysosomal glycogen. Late-onset Pompe disease can have onset at any age and sometimes mani fests in the elderly. It presents with a predominant proximal muscle weakness (limb girdle phenotype), moderately elevated serum creatine kinase and other muscle enzymes and respiratory dysfunction mainly due to a diaphragmatic weakness. Diagnosis requires low GAA activity in dried blood spots and genetic testing. The authors present the case of late-onset Pompe disease diagnosed in 45-year old male patient and discuss the efficacy of enzyme replacement therapy with recombinant GAA.

Correlation of Serum Calcium with Enzymes Aspartate Aminotransfrase, Creatine Kinase and Mastitis in Postpartum Period in Dairy Cattle

Mastitis is inflammation of udder and its chances increases in hypocalcemia cases because sphincters of teat are loosened due to calcium-ion channel blockage. Cows those suffered milk fever have 8 times more chances of developing mastitis than other cows which haven’t suffered hypocalcaemia. The values of AST and CK are high in downer cows due to hypocalcemia as compared to healthy cows. Subclinical hypocalcaemia is one of the major complication occurring just after parturition in local and exotic breeds of cattle in Pakistan. It weakens the immune system of dairy animal and also decreases the production potential of animals thus causing serious economic losses in terms of decrease milk production of animal, decrease earning of animal and increased treatment cost due to secondary infection like mastitis occurred due to weakened immune function. The current study was aimed to the correlation of serum calcium with enzymes Creatine Kinase and Aspartate amino transferase and mastitis in postpartum period in dairy cattle. A total of 50 cattle (n=50) just after parturition were included in this study. The cattle were sampled in two ways. Firstly, 10ml blood was drawn aseptically into clot activator vacutainers from jugular vein. Secondly milk samples were taken from all four teats. First 2 milk strips were discarded and next strips were collected in aseptic test tubes. The samples were transported to Pathology laboratory, Department of Pathology, University of Veterinary and Animal Sciences, Lahore maintaining the cold chain and stored at -20 0C till further analysis. Correlation between calcium and creatin kinase comes out to be 0.279 which is weak positive correlation. Correlation between calcium and aspartate aminotransferase is also weak and comes out to be 0.111. Similarly, correlation between calcium and SCC also weak and comes out to be 0.193.This is the first study of correlation of calcium with CK, AST and SCC in dairy cattle after postpartum period.&#x0D; &#x0D;

The effect of electrical stimulation of skeletal muscle on cardioprotection and on muscle-derived myokine levels in rats: A pilot study.

Electrical muscle stimulation (EMS) is a widely used method in sports and rehabilitation therapies to simulate physical exercise. EMS treatment via skeletal muscle activity improves the cardiovascular functions and the overall physical condition of the patients. However, the cardioprotective effect of EMS has not been proven so far, therefore, the aim of this study was to investigate the potential cardiac conditioning effect of EMS in an animal model. Low-frequency 35-min EMS was applied to the gastrocnemius muscle of male Wistar rats for three consecutive days. Their isolated hearts were then subjected to 30 min global ischemia and 120 min reperfusion. At the end of reperfusion cardiac specific creatine kinase (CK-MB) and lactate dehydrogenase (LDH) enzyme release and myocardial infarct size were determined. Additionally, skeletal muscle-driven myokine expression and release were also assessed. Phosphorylation of cardioprotective signaling pathway members AKT, ERK1/2, and STAT3 proteins were also measured. EMS significantly attenuated cardiac LDH and CK-MB enzyme activities in the coronary effluents at the end of the ex vivo reperfusion. EMS treatment considerably altered the myokine content of the stimulated gastrocnemius muscle without altering circulating myokine levels in the serum. Additionally, phosphorylation of cardiac AKT, ERK1/2, and STAT3 was not significantly different in the two groups. Despite the lack of significant infarct size reduction, the EMS treatment seems to influence the course of cellular damage due to ischemia/reperfusion and favorably modifies skeletal muscle myokine expressions. Our results suggest that EMS may have a protective effect on the myocardium, however, further optimization is required.

The Combined Effect of Taurine and Omega-3 Supplementation on Delayed Onset Muscle Soreness in High-Intensity Eccentric Exercise

Background: Eccentric exercise makes more tensions in active muscle fibers, resulting in muscle damage and delayed pain. Therefore, Delayed-Onset Muscle Soreness (DOMS) is a common result of severe eccentric contractions. This study aimed to clarify the combined effect of taurine and omega-3 supplementation on DOMS and muscle damage after high-intensity eccentric exercise in young untrained men.&#x0D; Methods: Forty-eight young untrained males were assigned to four groups (taurine+omega-3 [combined], taurine, omega-3, and one placebo group) and given 1,500 mg taurine and 1,500 mg omega-3 or 1,500 mg taurine or 1,500 mg omega-3 or placebo twice a day respectively. Each group received its supplements for twenty-eight days. DOMS and muscle damage in the lower body developed using a leg press with a weight equivalent to 70% of 1RM (One-Repetition Maximum). Lactate dehydrogenase (LDH), Creatine kinase (CK) and perceived muscle soreness measures were repeated before, immediately, 24, 48, and 72 hr after eccentric activity. p&lt;0.05 was considered to be statistically significant.&#x0D; Results: In the combined group, there was no increase in CK and LDH in immediate time and 72 hr after the exercise, respectively, compared to the period before the workout. At 24 and 48 hr after the training, there was a significant decrease in muscle pain perception in all groups compared to placebo (p&lt;0.05).&#x0D; Coclusion: A combination of 1500 mg taurine and 1500 mg omega-3, two times a day, for four weeks can reduce the levels of CK and LDH enzymes as some indicators of muscle damage. It also attenuates exercise-induced DOMS and muscle damage.

Skeletal muscle fibre type and enzymatic activity in adult offspring following placental and peripheral malaria exposure in foetal life.

Maternal malaria may restrict foetal growth. Impaired utero-placental blood flow due to malaria infection may cause hypoxia-induced altered skeletal muscle fibre type distribution in the offspring, which may contribute to insulin resistance and impaired glucose metabolism. This study assessed muscle fibre distribution 20 years after placental and/or peripheral in-utero malaria exposure compared to no exposure, i.e., PPM+, PM+, and M-, respectively. We traced 101 men and women offspring of mothers who participated in a malaria chemosuppression study in Muheza, Tanzania. Of 76 eligible participants, 50 individuals (29 men and 21 women) had skeletal muscle biopsy taken from m. vastus lateralis in the right leg. As previously reported, fasting and 30 min post-oral glucose challenge plasma glucose values were higher, and insulin secretion disposition index was lower, in the PPM+ group. Aerobic capacity (fitness) was estimated by an indirect VO2max test on a stationary bicycle. Muscle fibre sub-type (myosin heavy chain, MHC) distribution was analysed, as were muscle enzyme activities (citrate synthase (CS), 3-hydroxyacyl-CoA dehydrogenase, myophosphorylase, phosphofructokinase, lactate dehydrogenase, and creatine kinase activities. Between-group analyses were adjusted for MHC-I %. No differences in aerobic capacity were found between groups. Despite subtle elevations of plasma glucose levels in the PPM+ group, there was no difference in MHC sub-types or muscle enzymatic activities between the malaria-exposed and non-exposed groups. The current study did not show differences in MHC towards glycolytic sub-types or enzymatic activity across the sub-groups. The results support the notion of the mild elevations of plasma glucose levels in people exposed to placental malaria in pregnancy being due to compromised pancreatic insulin secretion rather than insulin resistance.

The Effects of Massage Therapy on Sport and Exercise Performance: A Systematic Review.

A massage is a tool that is frequently used in sports and exercise in general for recovery and increased performance. In this review paper, we aimed to search and systemize current literature findings relating to massages' effects on sports and exercise performance concerning its effects on motor abilities and neurophysiological and psychological mechanisms. The review has been written following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analysis) guidelines. One hundred and fourteen articles were included in this review. The data revealed that massages, in general, do not affect motor abilities, except flexibility. However, several studies demonstrated that positive muscle force and strength changed 48 h after the massage was given. Concerning neurophysiological parameters, the massage did not change blood lactate clearance, muscle blood flow, muscle temperature, or activation. However, many studies indicate pain reduction and delayed onset muscle soreness, which are probably correlated with the reduction of the level of creatine kinase enzyme and psychological mechanisms. In addition, the massage treatment led to a decrease in depression, stress, anxiety, and the perception of fatigue and an increase in mood, relaxation, and the perception of recovery. The direct usage of massages just for gaining results in sport and exercise performance seems questionable. However, it is indirectly connected to performance as an important tool when an athlete should stay focused and relaxed during competition or training and recover after them.

Application of enzymatically treated Artemisia annua L. on adult grass carp (Ctenopharyngodon idella): Improved growth performance, intestinal antioxidant capacity and apical junctional complex

The present study evaluated the effects of enzymatically treated Artemisia annua L. (EA) on the growth performance, intestinal antioxidant capacity, and apical junctional complex (AJC) of adult grass carp. In this experiment, 375 healthy grass carp (initial body weight 673.96 ± 1.24 g) were selected and randomly divided into 5 treatments, and fish in each group were fed diets with different levels of EA (0, 400, 800, 1200, and 1600 mg/kg) 9 weeks. The results showed that compared with the control group (0 mg/kg EA diet), the EA supplementation in the diet increased the percentage of weight gain (PWG), specific growth rate (SGR), and feed intake (FI) (P < 0.05), indicating that dietary EA supplementation improved the growth performance of adult grass carp. The dietary EA supplementation also enhanced the digestion and absorption ability of adult grass carp, which might be reflected by improving the activities of hepatopancreas digestive enzymes (trypsin, chymotrypsin, lipase, and amylase) and intestinal brush border enzymes (alkaline phosphatase, Na+/K+-ATPase, and γ-glutamyl transpeptidase), but EA did not affect the creatine kinase enzyme activity. Meanwhile, we explored the effect of dietary EA addition on the structural integrity of the grass carp intestine. We observed that dietary EA supplementation improved the antioxidant capacity of the intestine which be reflected by increasing the activities levels of superoxide dismutase (SOD) and catalase (CAT), which might be related to the binding protein Keap1a (not Keap1b)/ NF-E2-related factor-2 (Nrf2) signaling pathway. In addition, dietary EA supplementation enhanced the integrity of AJC by increasing the mRNA levels of intestinal tight junction proteins (TJs) (except claudin 11, claudin 12, claudin 15a) and adhere junction proteins (AJs) (E-cadherin, α-catenin, β-catenin, nectin, and afadin), which might be related to the Rho family GTPases (RhoA)/ Rho-associated protein kinase (ROCK)/ myosin light chain kinase (MLCK) signaling pathway. Collectively, dietary EA supplementation improved growth performance, digestion and absorption ability, and intestinal structural integrity in grass carp, which were associated with the activation of related signaling pathways and regulatory factors. Finally, by the growth indicator percent weight gain (PWG) and intestinal oxidative damage index protein carbonyl (PC), the optimum amounts of EA supplementation of adult grass carp (673–1726 g) were assessed to be 808.09 and 730.38 mg/kg diet, respectively.

Impact on cardioprotective effect of Psidium guajava leaves extract in streptozotocin-induced Wistar mice with molecular in silico analysis

Cardiovascular disease (CVD) and its complications have been regarded as the leading cause of morbidity and mortality. The drugs available in the market are effective to treat CVD, but with many adverse reactions. Nowadays, herbal products are the attention of researchers because of their less adverse effects. In this study, the cardioprotective effects of ethanolic leaves extract of Psidium guajava Linn. (Guava) (P. guajava) were evaluated in streptozotocin (STZ)-treated animal models. Mice acquired for the study were divided into five groups, each consisting of six mice. The toxin-induced mice were treated with the ethanolic leaves extract of P. guajava (300 mg/kg body weight [b.w.]). The results were compared to the standard drug (glibenclamide)-treated mice (3 mg/kg b.w.). The following parameters were considered for further investigations: creatine kinase-muscle brain (CK-MB), creatine kinase (CK), troponin, lysosomal, and mitochondrial enzymes. Then the docking study was accomplished. The levels of cardiac marker enzymes and lysosomal enzymes increased significantly in the toxin-induced mice, while the level of mitochondrial enzyme decreased significantly. During treatment with the ethanolic leaves extract of P. guajava, the levels of all parameters were notably reversed to normal range (P &lt; 0.05). Further, in docking analysis, the interaction of compounds, such as alpha-terpineol, cyclopentanecarboxamide, guaiol (a sesquiterpenoid alcohol), 1H-cyclopropanaphthalene, tetracyclotridecan-9-ol, dormin/abscisic acid, and epiglobulol, with the respective protein molecules, evidenced the cardioprotective effect of P. guajava leaves. Hence, it was concluded that the ethanolic leaves extract of P. guajava leaves have a cardioprotective effect.