Objective Rearch the changes of secretion and matrix metabolism in cartilage cells when the three protein component filaments of chondrocyte cytoskeleton (CSK) were destroyed.Methods 10 New Zealand white rabbits aged 2 months,We took the knee cartilage,obtain the cells using two-step enzymatic digestion and elimination method for cell culture.To be adherent cells,were randomly divided into four groups,namely normal control group,microfilament damage group ( adding 0.01 mg/L of cytochalasin-D destroy actin),microtubule damage group ( adding 0.4 mg/L colchicines destroy tubulin),intermediate filament damage group (adding 175 mg/L of acrylamide destroy vimentin).Cultured for 3 days,some cells from each group were digested and climbing films,line by immunofluorescence staining and confocal fluorescence microscope,semi-quantitative detection.The cells were observed actin protein tubulin and vimentin form and content.On the 3rd,6th and 9th day,obtained the cultured supernatant of each group detected type Ⅱ collagen and proteoglycan (GAG) content by ELISA method and Alcian blue.Results The composition of three cytoskeletal proteins,the average value of the corresponding analysis of fluorescence,showing microfilaments,microtubules,intermediate filaments destruction group compared with the control group fluorescence,fluorescence values were reduced from 83.70,82.82,77.93 to 60.45,57.86,55.56 ( P < 0.05,n =30). Meanwhile,the destruction of microtubules and intermediate fiber group on the 3rd,6th and 9th day,type Ⅱ collagen and GAG were significantly lower than the control group ( P<0.05 ),while the destruction of microfilaments group on the 3rd,6th and 9th day collagen Ⅱ and GAG content were not statistically different with control group ( P > 0.05 ).Conclusion The concentration of CSK default agents can destroy the CSK components.Chondrocyte matrix metabolism of microtubules and intermediate filament groups were significantly decreased,while microfilament destruction had no effect on cartilage matrix metabolism. Key words: Cartilage cells; Cytoskeleton; Collagen Ⅱ; Glycosaminoglycan