Understanding the marginal characteristics of ecosystem services (ESs) is crucial for sustainable management. However, there is limited research on quantifying thresholds for ESs, making it challenging for policymakers to plan effectively. This study established a framework to assess the influencing factors and marginal characteristics of cultural and regulatory ecosystem services (RES) in the Taihu Lake Basin of China and its wetland parks. The framework uses a Structural Equation Model to analyze the impact of visitors' attributes on the perception of different types of cultural ecosystem services (CES). It also employed the InVEST model to quantify the spatial patterns of RES in the basin. Multiple regression analysis and marginal utility theory were used to examine the influencing factors and marginal utilities for different types of cultural and regulatory services. The findings reveal that 1) Education level, income, gender, and age significantly influence the perception of five types of CES provision, with different factors affecting each type. 2) Most park characteristics influence the CES provision score, with their maximum and marginal utility peaks. For instance, as patch density (PD) increases, the score for recreational services initially decreases, then increases, and finally decreases again, reaching a peak of 0.50 at a PD of 325.60. The marginal utility of PD follows a similar pattern, initially increasing and then decreasing, with an increase in utility observed in the range of 0 < PD < 187.80. When PD reaches 187.80, it attains its maximum utility, indicating the point of highest efficiency for PD's impact on recreational services. 3) Heterogeneity exists in the spatial distribution of RES within the basin, with different landscape features providing varying marginal benefits for services like water supply, water purification, carbon storage, and soil erosion prevention. Carbon storage demonstrates an 'increase-then-decrease’ pattern in response to rising FP (Percentage of forest area), reaching a maximum value of 13129.3 t/km2 at FP of 66.4. The marginal utility of FP also exhibits a 'rise-then-fall’ trend, increasing up to FP of 30.4, where it peaks at 230.5. This peak represents the point of maximum efficiency in FP's influence on carbon storage services. 4) The effects of park and landscape characteristics on cultural and regulatory services exhibit marginal utilities that can be used to determine the optimal scale and location of these features to maximize ecological benefits. This research aims to expand the scope of ESs assessment and provide insights from wetland parks for broader environmental planning and optimization.