Environmental Adaptation Research Articles

Research on flexible piezoresistive sensors based on nanocomposite materials

As a core component, sensors play a crucial role in fields such as the Internet of Things, biomedicine, and artificial intelligence by converting physical information into electrical signals. With technological advancements, the demand for improved sensor performance and environmental adaptability has been increasing, driving progress in advanced materials, new structures, and refined processing technologies to enhance the efficiency of converting pressure signals into electrical signals. Flexible piezoresistive sensors are widely used due to their high sensitivity, good linear response, and fast response time. The performance of these sensors heavily depends on the microstructure of the materials, and traditional detection methods struggle to capture their dynamic changes. Therefore, developing in-situ characterization techniques to monitor changes in material structure and chemical properties in real-time is crucial for optimizing material performance. Additionally, integrated sensor systems that study signal acquisition, circuit interfaces, and signal processing, combined with artificial intelligence algorithms for data processing and analysis, not only improve system efficiency but also broaden their application range.

Ultrahigh Stretchable, Highly Transparent, Self-Adhesive, and Environment-Tolerant Chitin Nanocrystals Engineered Eutectogels toward Multisignal Sensors.

Addressing the conflict between achieving elevated mechanical stretchability and environmental adaptability is significant to a breakthrough in the practical application of flexible wearable materials. Therefore, inspired by the perceptive and protective properties of human skin, flexible wearable electronic skins (E-skins) based on deep eutectic solvent (DES) liquid and multiresponse eutectogel have been widely considered to be a promising platform for building a flexible wearable management system to achieve the purpose of "one stone, two birds". In this work, a multifunctional E-skin was designed based on an ultrastretchable, transparent, self-adhesive, and environmentally tolerant eutectogel by first incorporating cationized modified chitin nanocrystals into a covalently cross-linked polymer network comprised of the skeleton formed by a PAA polymerization network structure serving as a stretchable matrix and filled with DESs (ChCl:EG). The obtained eutectogel exhibits superhigh stretchability (up to 6707%), high toughness (17.7 MJ/m3), mechanical strength (0.48 MPa), self-adhesive, and high transparency (91.2%). Simultaneously, the multisignal sensor based on the above comprehensive properties and thermosensitive capacity exhibits a wide monitoring range, high strain/compression/temperature sensitivity, and good reproducibility. Remarkably, the sensor could be attached to rat hearts without glue or stickers for long-term monitoring of high-quality in vivo heartbeat signals. In this way, it is believed that the designed E-skin system based on eutectogel has great potential to serve as a promising platform for the next generation of flexible multisignal monitoring integrated wearable management systems.

Annelid Comparative Genomics and the Evolution of Massive Lineage-Specific Genome Rearrangement in Bilaterians.

The organization of genomes into chromosomes is critical for processes such as genetic recombination, environmental adaptation, and speciation. All animals with bilateral symmetry inherited a genome structure from their last common ancestor that has been highly conserved in some taxa but seemingly unconstrained in others. However, the evolutionary forces driving these differences and the processes by which they emerge have remained largely uncharacterized. Here, we analyze genome organization across the phylum Annelida using 23 chromosome-level annelid genomes. We find that while many annelid lineages have maintained the conserved bilaterian genome structure, the Clitellata, a group containing leeches and earthworms, possesses completely scrambled genomes. We develop a rearrangement index to quantify the extent of genome structure evolution and show that, compared to the last common ancestor of bilaterians, leeches and earthworms have among the most highly rearranged genomes of any currently sampled species. We further show that bilaterian genomes can be classified into two distinct categories-high and low rearrangement-largely influenced by the presence or absence, respectively, of chromosome fission events. Our findings demonstrate that animal genome structure can be highly variable within a phylum and reveal that genome rearrangement can occur both in a gradual, stepwise fashion, or rapid, all-encompassing changes over short evolutionary timescales.

Analysis of Maize Planting Mode and Simulation and Optimization of Ridging and Fertilization Components in Arid Area of Northwest China

The arid area of Northwest China belongs to the rain-fed agricultural area of the Loess Plateau, and water resources have become one of the important factors limiting agricultural development in this area. This study employed the AquaCrop model to predict the yield advantages and environmental adaptability of maize in Dingxi City from 2016 to 2020 under two cultivation practices: ridge tillage (100% film coverage with double ridge-furrow planting) and flat planting (81.8% film coverage with wide-film planting). The numerical simulation of the tillage and fertilization process of the double-ridge seedbed was carried out by EDEM, and the key components were tested by the Box–Behnken center combination test design principle to obtain the optimal parameter combination. The results showed that ridge planting was more suitable for agricultural planting in rain-fed arid areas in Northwest China. The simulation analysis of ridging and fertilization showed that the forward speed of the combined machine was 0.50 m/s, the rotation speed of the trough wheel of the fertilizer discharger was 39 rmp, and the rotary tillage depth was 150 mm. The qualified rate of seedbed tillage was 93.6%, and the qualified rate of fertilization was 92.1%. The research shows that the whole-film double ridge-furrow sowing technology of maize is more suitable for the rain-fed agricultural area in the arid area of Northwest China. The simulation results of the ridging fertilization device are consistent with the field experiment results. The research results provide a certain technical reference for the optimization of the whole-film double ridge-furrow sowing technology.

Population genetic diversity and environmental adaptation of Tamarix hispida in the Tarim Basin, arid Northwestern China.

Arid ecosystems, characterized by severe water scarcity, play a crucial role in preserving Earth's biodiversity and resources. The Tarim Basin in Northwestern China, a typical arid region isolated by the Tianshan Mountains and expansive deserts, provides a special study area for investigating how plant response and adaptation to such environments. Tamarix hispida, a species well adapted to saline-alkaline and drought conditions, dominates in the saline-alkali lands of the Tarim Basin. This study aims to examine the genetic diversity and environmental adaptation of T. hispida in the Tarim Basin. Genomic SNPs for a total of 160 individuals from 17 populations were generated using dd-RAD sequencing approach. Population genetic structure and genetic diversity were analyzed by methods including ADMIXTURE, PCA, and phylogenetic tree. Environmental association analysis (EAA) was performed using LFMM and RDA analyses. The results revealed two major genetic lineages with geographical substitution patterns from west to east, indicating significant gene flow and hybridization. Environmental factors such as Precipitation Seasonality (bio15) and Topsoil Sand Fraction (T_SAND) significantly shaped allele frequencies, supporting the species' genetic adaptability. Several genes associated with environmental adaptation were identified and annotated, highlighting physiological and metabolic processes crucial for survival in arid conditions. The study highlights the role of geographical isolation and environmental factors in shaping genetic structure and adaptive evolution. The identified adaptive genes related to stress tolerance emphasize the species' resilience and highlight the importance of specific physiological and metabolic pathways.

Chromosome-Level Assembly Reveals a Fifteen-Chromosome Aneuploid Genome and Environmental Adaptation Strategy of Chinese Traditional Medical Fungus Wolfiporia hoelen.

The sclerotia of Wolfiporia hoelen are one of the most important traditional Chinese medicines and foods commonly used in China, Japan, Korea, and other Asian countries. To provide a high-quality reference genome and deepen our understanding of the genome of W. hoelen to elucidate various biological phenomena. In this study, we assembled three genomes of W. hoelen using a combination of Nanopore and Illumina sequencing strategies. The fifteen-chromosome genome L7 of W. hoelen was assembled with two-sided telomere and rDNA sequences for the first time. The chromosome count was subsequently confirmed through collinearity analysis, correcting the previous belief that W. hoelen had only fourteen chromosomes. Moreover, the aneuploid genome was discovered in W. hoelen for the first time through sequencing depth analysis of different chromosomes, and only some strains of W. hoelen exhibit aneuploid genomes. According to the genome analysis of homokaryotic offspring and protoplast-isolated strains, a potential variation in chromosome allocation patterns was revealed. Moreover, the gene function enrichment analysis of genes on reduplicated chromosomes demonstrated that aneuploidy in the genome may be the result of environmental adaptation for W. hoelen. The discovery of an aneuploid genome also provides new ideas for genetic improvement of W. hoelen.

Comparative analysis of environmental adaptation and individual strategy choice in animal foraging behavior

Comparative analysis of environmental adaptation and individual strategy choice in animal foraging behavior

Complex evolutionary patterns within the tubulin gene family of ciliates, unicellular eukaryotes with diverse microtubular structures

BackgroundTubulins are major components of the eukaryotic cytoskeletons that are crucial in many cellular processes. Ciliated protists comprise one of the oldest eukaryotic lineages possessing cilia over their cell surface and assembling many diverse microtubular structures. As such, ciliates are excellent model organisms to clarify the origin and evolution of tubulins in the early stages of eukaryote evolution. Nonetheless, the evolutionary history of the tubulin subfamilies within and among ciliate classes is unclear.ResultsWe analyzed the evolutionary pattern of ciliate tubulin gene family based on genomes/transcriptomes of 60 species covering 10 ciliate classes. Results showed: (1) Six tubulin subfamilies (α_Tub, β_Tub, γ_Tub, δ_Tub, ε_Tub, and ζ_Tub) originated from the last eukaryotic common ancestor (LECA) were observed within ciliates. Among them, α_Tub, β_Tub, and γ_Tub were present in all ciliate species, while δ_Tub, ε_Tub, and ζ_Tub might be independently lost in some species. (2) The evolutionary history of the tubulin subfamilies varied. Evolutionary history of ciliate γ_Tub, δ_Tub, ε_Tub, and ζ_Tub showed a certain degree of consistency with the phylogeny of species after the divergence of ciliate classes, while the evolutionary history of ciliate α_Tub and β_Tub varied among different classes. (3) Ciliate α- and β-tubulin isoforms could be classified into an “ancestral group” present in LECA and a “divergent group” containing only ciliate sequences. Alveolata-specific expansion events probably occurred within the “ancestral group” of α_Tub and β_Tub. The “divergent group” might be important for ciliate morphological differentiation and wide environmental adaptability. (4) Expansion events of the tubulin gene family appeared to be consistent with whole genome duplication (WGD) events in some degree. More Paramecium-specific tubulin expansions were detected than Tetrahymena-specific ones. Compared to other Paramecium species, the Paramecium aurelia complex underwent a more recent WGD which might have experienced more tubulin expansion events.ConclusionsEvolutionary history among different tubulin gene subfamilies seemed to vary within ciliated protists. And the complex evolutionary patterns of tubulins among different ciliate classes might drive functional diversification. Our investigation provided meaningful information for understanding the evolution of tubulin gene family in the early stages of eukaryote evolution.

Research on Applications of Image Recognition in the Design of Autonomous Navigation Robots

This study evaluates traditional and deep learning-based image recognition technologies in autonomous navigation robots, detailing their strengths and limitations. Traditional image recognition techniques, which rely on predefined algorithms and pattern recognition, have proven efficient and stable for navigation in controlled environments. Conversely, deep learning approaches, notably through convolutional neural networks (CNNs), excel in dynamic and unpredictable settings by adapting more effectively to complex environmental interactions. The core challenges in this field include the need for real-time data processing, achieving consistent accuracy across diverse environments, and managing the computational demands of sophisticated algorithms. This research highlights the significant improvements deep learning techniques bring to autonomous navigation, particularly in terms of adaptability and robustness. The study also addresses the necessity for advancements in both technology domains to meet the evolving demands of autonomous robotics, emphasizing the ongoing need to enhance computational efficiency and environmental adaptability. The findings suggest a growing potential for future research to explore hybrid models that leverage the predictability of traditional methods with the flexibility of deep learning to optimize autonomous navigational tasks.

Germination characteristics associated with nicosulfuron resistance in Amaranthus retroflexus L.

Nicosulfuron-resistant biotype (R) and -sensitive biotype (S) Amaranthus retroflexus L. seeds were subjected to different temperature, light, salt, osmotic potential, pH value and burial depth treatments. The difference in germination response of two populations to the above abiotic environmental factors was used to study the fitness cost of nicosulfuron-resistance evolution in A. retroflexus. The aim is to find a powerful tool for weed control in the presence of evolutionary resistance selection. The results of this experiment showed that the germination rate and germination index in S population were generally higher than that in R population. When the salt stress was 80 mM, the water potential was -0.1 Mpa ~ -0.4 Mpa, and under strong acid and alkali conditions, the germination index in S population was prominently higher than that in R population (p<0.05). The delayed seed germination in R population indicated that its nicosulfuron resistance may be linked to seed biochemical compositions that altered seed germination dynamics. The resistant and sensitive biotype of A. retroflexus had differently favourable adaptability in diverse environments. Salt, osmotic potential and pH value are not the major constraints for A. retroflexus germination, however, A. retroflexus are strongly responsive to temperature, light and burial depth. Considering that seeds of A. retroflexus are unable to reach the soil surface beyond the depth of 6 cm, deep inversion tillage before sowing may be an effective and economical weed management tool for the control of nicosulfuron resistant A. retroflexus.

Predictive Functional Profiling Reveals Putative Metabolic Capacities of Bacterial Communities in Drinking Water Resources and Distribution Supply in Mega Manila, Philippines

Assessing bacterial communities across water resources is crucial for understanding ecological dynamics and improving water quality management. This study examines the functional profiles of bacterial communities in drinking water resources in Mega Manila, Philippines, including Laguna Lake tributaries, pre-treatment plant sites, groundwater sources, and post-treatment plant sites. Using eDNA sequencing, flux balance analysis, and taxonomy-to-phenotype mapping, we identified metabolic pathways involved in nutrient metabolism, pollutant degradation, antibio- tic synthesis, and nutrient cycling. Despite site variations, there are shared metabolic pathways, suggesting the influence of common ecological factors. Site-specific differences in pathways like ascorbate, aldarate, and phenylalanine metabolism indicate localized environmental adaptations. Antibiotic synthesis pathways, such as streptomycin and polyketide sugar unit biosynthesis, were detected across sites. Bacterial communities in raw and pre-treatment water showed potential for pollutant degradation such as for endocrine-disrupting chemicals. High levels of ammonia-oxidizing and sulfate-reducing bacteria in pre- and post-treatment water suggest active nitrogen removal and pH neutralization, indicating a need to reassess existing water treatment approaches. This study underscores the adaptability of bacterial communities to environmental factors, as well as the importance of considering their functional profiles in assessing drinking water quality resources in urban areas.

Photosystem I: A Paradigm for Understanding Biological Environmental Adaptation Mechanisms in Cyanobacteria and Algae.

The process of oxygenic photosynthesis is primarily driven by two multiprotein complexes known as photosystem II (PSII) and photosystem I (PSI). PSII facilitates the light-induced reactions of water-splitting and plastoquinone reduction, while PSI functions as the light-driven plastocyanin-ferredoxin oxidoreductase. In contrast to the highly conserved structure of PSII among all oxygen-evolving photosynthetic organisms, the structures of PSI exhibit remarkable variations, especially for photosynthetic organisms that grow in special environments. In this review, we make a concise overview of the recent investigations of PSI from photosynthetic microorganisms including prokaryotic cyanobacteria and eukaryotic algae from the perspective of structural biology. All known PSI complexes contain a highly conserved heterodimeric core; however, their pigment compositions and peripheral light-harvesting proteins are substantially flexible. This structural plasticity of PSI reveals the dynamic adaptation to environmental changes for photosynthetic organisms.

Efficient bioremediation of multiple steroid hormones by halotolerant 17β-hydroxysteroid dehydrogenase derived from moderately halophilic Pontibacillus chungwhensis HN14.

Steroid hormones exhibit potent endocrine disrupting activity and have been shown to disrupt the equilibrium of aquatic ecosystems and pose a threat to public health through their persistent and carcinogenic effects. Pontibacillus chungwhensis HN14, a moderately halophilic bacterium with the capacity to effectively degrade various polycyclic aromatic hydrocarbons and other organic pollutants, was previously isolated. Additionally, the strain HN14 showed strong environmental adaptability under various environmental stress conditions. In this study, the steroid degradation by strain HN14 was studied for the first time. We demonstrated that strain HN14 could degrade estradiol (E2) to maintain the growth of the strain and could convert E2 to estrone. Additionally, the efficient substrate degradation efficiency of P. chungwhensis HN14 under high salinity and high substrate concentration conditions was demonstrated. Furthermore, a 17β-hydroxysteroid dehydrogenase, 17β-HSD(HN14), was identified in strain HN14. Comparative analysis reveals that 17β-HSD(HN14) shares approximately 38% sequence identity with 17β-HSDx from Rhodococcus sp. P14. In addition, 100µg of purified 17β-HSD(HN14) could effectively convert about 40% of 0.25 mM of E2 within 1h period, with an enzyme activity of 17.5 U/mg, and catalyze the dehydrogenation of E2 and testosterone at the C-17 position. The characterization of purified enzyme properties reveals that 17β-HSD(HN14) exhibits exceptional structural robustness and enzymatic efficacy even under high salinity conditions of up to 20%. Overall, this study enhances our comprehension of steroid biodegradation in strain HN14 and contributes novel ideas and theoretical underpinnings for advancing bioremediation technologies targeting steroid pollution in high-saline environments.

Pediatric Bobath Concept in management of children with cerebral palsy: view of Turkish Bobath therapists

Purpose: Pediatric Bobath Concept (PBC) is a family-child centered holistic concept applied interdisciplinary to support the functional skills of children with cerebral palsy. The aim of this study was to examine the effects of PBC on children, parents, and themselves from the perspective of Bobath therapists in Turkey. Methods: In this observational study, 104 (69.2% female) Bobath therapists with a mean age of 37.9+6.1 years participated. The Pediatric-Bobath Impact Questionnaire (P-BIQ) was developed by the Delphi method. Content validity (Lawshe's Content Validity Index-CVI) and test-retest reliability (Intraclass correlation coefficient -ICC) were examined. The relationships between the rates of change in children, parents, and therapists were analyzed using the Chi Square (χ2) test and the effect sizes of the relationships were analyzed according to Cramer's V values. Results: According to the P-BIQ (CVI=0.836, ICC &gt;0.737), at least 74% of therapists reported that the PBC positively affected children (compliance with therapy and home program, body structure and function, activity and participation, environmental adaptations), parents (compliance with therapy and home program) and themselves (sense of professional competence and motivation). Compliance of the child, parent, and therapist was positively associated with the child's activity and participation levels and environmental adaptation (p

Exploring The Effects Of Entrepreneurship Competency And Environmental Adaptability On Marketing Performance: Business Strategy Mediation In Himki Jepara

Abstract . This research investigates the influence of entrepreneurial competence and the ability to adapt to the environment on marketing performance in Small and Medium Enterprises (SMEs) in the furniture industry sector in Jepara, by considering the mediating role of business strategy. In the midst of globalization and intense competition, entrepreneurial competence, which includes managerial ability, innovation and risk taking, as well as environmental adaptability, which includes flexibility and response to market changes, have become key factors in improving marketing performance. This research uses a quantitative approach with correlational study methods and Partial Least Squares (PLS) statistical techniques to analyze data from 194 respondents who are members of the Jepara Indonesian Furniture and Crafts Industry Association (HIMKI). The research results show that entrepreneurial competence and environmental adaptability have a positive and significant influence on marketing performance. In addition, business strategy functions as a mediator in the relationship between entrepreneurial competence and marketing performance as well as between environmental adaptability and marketing performance. These findings emphasize the importance of developing entrepreneurial competence and adaptability as factors that not only have a direct impact on marketing performance but also through more effective business strategies. This research provides valuable insights for furniture entrepreneurs in Jepara in optimizing their marketing performance in a dynamic and competitive business environment.

An immune optimization deep reinforcement learning control method used for magnetorheological elastomer vibration absorber

On-board precision equipment installed in vehicles is susceptible to vibrations caused by irregularities on the road surface, which may decrease accuracy and damage to components. Installing a vibration absorption system in the vehicle is necessary to mitigate the impact of vehicle vibrations on the equipment. Vibration systems widely use vibration absorbers based on magnetorheological elastomer (MRE). Previous research has mainly used Proportional-Integral-Derivative (PID), fuzzy control, and ON-OFF algorithms to control the vibration absorption system. However, these algorithms exhibit poor control accuracy and adaptability in uncertain environments. To enhance the adaptive capabilities of vibration absorbers in time-varying environments and to maximize their performance, this study explores the use of the Twin Delayed Deep Deterministic Policy Gradient (TD3) control algorithm. Additionally, we proposed an Immune Optimization Twin Delayed Deep Deterministic Policy Gradient (IO-TD3) control algorithm to address the low-efficiency issue in the initial training stages. Simulation results show that, compared to the TD3 algorithm, Double Q-Deep Deterministic Policy Gradient (Double Q-DDPG) algorithm, Deep Deterministic Policy Gradient (DDPG) algorithm, and Deep Q-Network (DQN) algorithm, the IO-TD3 algorithm significantly exhibits faster convergence speed and improved stability. Finally, we apply the IO-TD3 algorithm to the MRE absorber controller and verify the controller's performance using the Monte Carlo method. The results show that the controller using the IO-TD3 control algorithm has high control accuracy and stability.

NUMERICAL INVESTIGATION OF A OMNIDIRECTIONAL BENDING ACTUATOR FOR CLIMBING ANALYSIS

With its good flexibility and adaptability to the environment, soft robot has been attached great importance by the majority of researchers in recent years, and is widely used in rehabilitation treatment, flexible grasping and other fields. However, the existing actuators have disadvantages such as single deformation mode, poor flexibility and insufficient environmental adaptability. The reason is that the actuator structure is too simple to meet the complex application scenarios. In this paper, an omnidirectional bending multi-segment soft actuator is proposed, and the parameter analysis and dynamic analysis models are given. Meanwhile, the prestressed modal analysis of the proposed model is carried out to better characterize the dynamic behavior. A numerical study of a climbing motion verifies the novelty and validity of the proposed structure.

The LTAR Cropland Common Experiment at Central Mississippi River Basin.

The Central Mississippi River Basin (CMRB) Common Experiment, with its marginal soils and southern Corn Belt climate, is an ideal location for evaluating progress toward environmental, productivity, and climatic adaptation goals. Sustainable production with conventional row-crop systems is more challenging than in the upper Corn Belt, making evaluation and adoption of alternative farming practices crucial. This Common Experiment has a hydrologically restrictive layer causing reduced plant available water capacity in the root zone. The CMRB site joined the Long-Term Agroecosystem Research Network in 2011 with the Cropland Common Experiment established in 2015. The Common Experiment contrasts prevailing and alternative practices at plot and field scale. Improvement of the soil ecosystem is key, as it underpins other objectives, including reduced nutrient losses, increased soil water holding capacity, and yield stability.

A Falls Prevention Program for People After Stroke in Guyana: An International Collaboration.

The objective was to describe the social, environmental, and cultural adaptations to an existing falls program and assess acceptability and preliminary effectiveness of the program in reducing fear, reducing falls, and improving function among individuals poststroke in Guyana. A quasi-experimental pilot study with a pretest/ posttest in-group design was developed through a collaboration of researchers in Guyana and the US. Participants took part in the falls prevention program for 8weeks. Outcome measures included a 10-meter walk test, Five Times Sit to Stand, and subjective questionnaires for falls incidence and balance confidence at the beginning and end. Twenty participants completed the study. One participant experienced medical complications and their data were excluded from analysis. Fifteen participants (78.9%) demonstrated improvements in comfortable and fast walking speed. Twelve participants completed the Five Times Sit to Stand Test. Eleven (91.67%) improved their time at posttest, with 9 (81.8%) demonstrating a clinically important improvement. Nineteen participants had sustained at least 1 fall prior to the study. Only 1 participant reported a fall during the program. Initially, the majority of participants (11/19) were very concerned about falling. At the end, only 1 was very concerned about falling, and the majority (15/19) were not concerned at all. Post-test surveys of participants indicated acceptability of the program. This pilot program helped reduce fall risk and improve confidence, gait speed and community mobility of the study participants. Future research at other rehabilitation departments in Guyana would help increase generalizability of the program. The program can be used clinically by physical therapists in Guyana both in departments and as a home program. Shared knowledge and experience of researchers considering research evidence and the environmental, social, and economic conditions of people living in Guyana were important in developing an effective program.

De novo genome assembly of white clover (Trifolium repens L.) reveals the role of copy number variation in rapid environmental adaptation

BackgroundWhite clover (Trifolium repens) is a globally important perennial forage legume. This species also serves as an eco-evolutionary model system for studying within-species chemical defense variation; it features a well-studied polymorphism for cyanogenesis (HCN release following tissue damage), with higher frequencies of cyanogenic plants favored in warmer locations worldwide. Using a newly generated haplotype-resolved genome and two other long-read assemblies, we tested the hypothesis that copy number variants (CNVs) at cyanogenesis genes play a role in the ability of white clover to rapidly adapt to local environments. We also examined questions on subgenome evolution in this recently evolved allotetraploid species and on chromosomal rearrangements in the broader IRLC legume clade.ResultsIntegration of PacBio HiFi, Omni-C, Illumina, and linkage map data yielded a completely de novo genome assembly for white clover (created without a priori sequence assignment to subgenomes). We find that white clover has undergone extensive transposon diversification since its origin but otherwise shows highly conserved genome organization and composition with its diploid progenitors. Unlike some other clover species, its chromosomal structure is conserved with other IRLC legumes. We further find extensive evidence of CNVs at the major cyanogenesis loci; these contribute to quantitative variation in the cyanogenic phenotype and to local adaptation across wild North American populations.ConclusionsThis work provides a case study documenting the role of CNVs in local adaptation in a plant species, and it highlights the value of pan-genome data for identifying contributions of structural variants to adaptation in nature.