A 61-m-long sediment core (HB-1) and 690 km of high-resolution seismic profiles from offshore of the Yellow River delta, Bohai Sea, were analyzed to document the stratigraphy and sea-level changes during the Late Pleistocene and Holocene. Accelerator mass spectrometry 14C dating and analyses of benthic foraminifera, ostracods, the mineral composition, and sedimentary characteristics were performed for core HB-1, and seven depositional units (DU 1–DU 7 in descending order) were identified. The seismic reflection data were interpreted in light of the sedimentological data of the core and correlated with other well-studied cores obtained previously in the Bohai Sea area. Seven seismic units (SU 1 to SU 7 in descending order) were distinguished and interpreted as follows: SU 7 corresponds to marine facies in Marine Isotopic Stage (MIS) 5; SU 6, to terrestrial facies in MIS 4; SU 5 and SU 4, to alternating terrestrial and marine facies (DU 7–DU 5) in MIS 3; SU 3, to terrestrial facies (DU 4) in MIS 2; SU 2, to Holocene marine facies (DU 3 and DU 2); and SU 1, to modern Yellow River delta sediments deposited since 1855 (DU 1). The sedimentary facies from DU 7 to DU 5 reflect sea-level fluctuations during MIS 3, and the boundary between DU 5 and DU 6, which coincides with that between SU 4 and SU 5, is a distinctive, laterally continuous, undulating erosion surface, with up to ∼20 m of relief. This surface reflects subaerial exposure between transgressions during MIS 3. Estimated sea levels during MIS 3 ranged from −35 ± 5 to −60 ± 5 m or lower, with short-term fluctuations of ∼20 m. Sedimentary environments in the Bohai Sea area were governed mainly by eustatic sea-level changes and the Bohai Strait topography, which controls the entry of sea water into the Bohai Sea area. The mineral composition of the sediment core suggests that the Yellow River did not discharge into the Bohai Sea, or at least did not influence the study area significantly, during parts of MIS 3 and MIS 2 to the early Holocene (∼11–8.5 cal kyr BP).