In recent years, the need for energy security strategies through liquefied natural gas (LNG) import has occupied an unprecedented spot in the European Union's foreign policy agenda. The availability of abundant natural gas resources in Western Canada, making this region a potential supplier, has, therefore, received significant attention. In order to ensure a competitive spot in the global natural gas market, it is important for Canada to supply its natural gas both at a competitive price and with lower emissions. In this study, a comparative assessment of the delivered costs and life cycle greenhouse gas (GHG) emissions of the natural gas supply chain from production sites in Canada to north and southwest Europe is conducted through the development of techno-economic and life cycle analyses models. Two possible supply chain routes to Europe were explored, one from the west coast and the other via the east coast of Canada, and included recovery, processing, transmission, liquefaction, shipping, and re-gasification. Two sources of Canadian natural gas reserves, Montney and Horn River, are investigated. The results show that the delivered cost ($/GJ) of Canadian LNG (including recovery, processing, transmission, liquefaction, and shipping cost) to Europe is 8.9–12.9, depending on the resources and pathway. The total well-to-port (WTP) GHG emissions (including emissions from recovery, processing, transportation, liquefaction, shipping and re-gasification at the destination port) from the Canadian production sites to Europe is 22.9–42.1 g-CO2eq/MJ, depending on the resources and pathway followed. The costs and GHG emissions values reported in the literature for the delivery of natural gas from the major exporting countries were lower than those for the Canadian LNG supply chain. Finding other sources of natural gas in Eastern Canada might provide a cheaper and less GHG-intensive alternative to the Canadian LNG supply chain.