To gain a comprehensive understanding of the intrinsic characteristics of the lock-in region of the π-shaped section, a study was conducted to investigate the influence of the Angle of Attack (AOA), damping ratio, and mass ratio on the characteristics of the two lock-in regions using a 1:50 segment model test. The Proper Orthogonal Decomposition (POD) method was employed to analyze the characteristics of the two lock-in regions, and the flow field difference between the two lock-in regions was analyzed using Computational Fluid Dynamics (CFD). The findings indicate that both the Angle of Attack (AOA) and mass ratio have comparable effects on the lock-in region. However, it is noteworthy that the AOA appears to exert a more pronounced influence on the second lock-in region, whereas the damping ratio predominantly impacts the amplitude of the lock-in region. The analysis of the distribution and jumping degree of the surface time-averaged pressure enables us to infer the key-action zone number and action position of vortices present around the given section. In the two-lock-in region, there exist two different distribution forms for the energy ratio of POD modes. The first lock-in region is primarily affected by the Karman vortex street phenomenon, whereas the second lock-in region is mainly influenced by the long tail vortex shedding.