End Groups Research Articles

In Situ Efficient End Functionalization of Polyisoprene by Epoxide Compounds via Neodymium-Mediated Coordinative Chain Transfer Polymerization.

The Nd-mediated coordinative chain transfer polymerization (CCTP) of dienes represents one of the state-of-the-art techniques in the current synthetic rubber field. Besides having well-controlled polymerization behaviors as well as high atom economies, it also allows for the generation of highly reactive Al-capped polydienyl chain-ends, which hold great potential, yet much less explored up to date, in achieving end functionalization to mimic the structure of natural rubber. In this study, we demonstrate an efficient in situ method to realize end-functionalizing polyisoprene by introducing epoxide compounds into a CCTP system. The end functionalization efficiency was 92.7%, and the obtained polymers were systematically characterized by 1H NMR, 1H,1H-COSY NMR, DOSY NMR, and MALDI TOF. NMR studies revealed that a maximum of two EO units were introduced to the chain ends, and based on density functional theory (DFT) studies, an energy barrier of 33.3 kcal/mol was required to be overcome to open the ring of the EO monomer. Increasing the ratio of [Ip]/[Nd] resulted in gradually increased viscosities for the reaction medium and therefore gave rise to an end functionalization efficiency that decreased from 92.7% to 74.2%. The end hydroxyl group can also be readily converted to other functionalities, as confirmed by NMR spectroscopy.

Tunable Emission and Structural Insights of 6-Arylvinyl-2,4-bis(2'-hydroxyphenyl)pyrimidines and Their O∧N∧O-Chelated Boron Complexes.

In this study, we present the synthesis and photophysical characteristics of a novel series of 6-arylvinyl-2,4-bis(2'-hydroxyphenyl)pyrimidines. These compounds exhibit nonemissive properties attributed to the potential occurrence of a excited-state intramolecular proton transfer process from the OH groups to the nitrogen atoms of the pyrimidine ring. The introduction of an acid for protonation of the pyrimidine ring results in a significant enhancement of the fluorescence response, easily perceptible to the naked eye. Notably, these molecules serve as intriguing rigid O∧N∧O ligands for boron chelation. The incorporation of boron atoms promotes structural planarity, increases rigidity, and successfully restores fluorescence in both solution and the solid state. Moreover, the photoluminescence was found to be strongly influenced by the nature of the end groups on the arylvinylene fragment, allowing for the modulation of the emission color and covering the optical spectrum from blue to red. Strong emission solvatochromism was observed in various solvents, a finding that supports the formation of intramolecular charge-separated emitting states, particularly when terminal electron-donating groups are present in the structure. X-ray diffraction analysis enables the determination of inter- and intramolecular interactions, as well as molecular packing structures, aiding in the rationalization of distinct luminescent behaviors in the solid state. All experimental findings are elucidated through extensive density functional theory (DFT) and time-dependent DFT calculations.

Optimizing non-fullerene acceptor molecules constituting fluorene core for enhanced performance in organic solar cells: a theoretical methodology.

Looking for novel outstanding performance materials suitable for organic solar cells, we constructed a range of non-fullerene acceptors (NFAs) evolved from the recently synthesized acceptor molecule identified as DICTIF, structured around fluorene core where 2-(2,3-dihydro-3-oxo-1H-inden-1-ylidene) propanedinitrile presented the terminals end-groups. Employing density functional theory (DFT) and time dependent-DFT (TD-DFT) simulations, we have simulated the impact of altering the end groups of DICTIF molecule by five assorted acceptors molecules, for the purpose of exploring their opto-electronic properties and their performance in organic solar cell (OSC) applications. We proved that the designed non-fullerene acceptors provide enhanced efficiency compared to the synthesized molecule, such as planar geometries and narrower energy gap ranging from 1.51 to 1.95 eV. A red shift in absorption was observed for all tailored molecules (λmax = 583.5-711.4 nm) as compared to the reference molecule (λmax = 578 nm).Various decisive factors such as frontier molecular orbitals (FMOs), exciton binding energy (EB), absorption maximum (λmax), open circuit voltage (VOC), reorganization energies (RE), transition density matrix (TDM), reduced density gradient (RDG), and electron-hole overlap have also been computed for analyzing the performance of NFAs. Low reorganizational energy values facilitate charge mobility which improves the conductivity of all the designed molecules. This study showed that our novel tailored molecules might be suitable candidates for the fabrication of highly efficient photovoltaic materials. After testing various hybrid functionals, optimized geometries were assigned using DFT HSEH1PBE/6-31G(d) level of theory. Electronic excitations and absorption spectra were investigated using the TD-DFT MPW1PW91/6-31G(d) level of theory. We ascertained that HSEH1PBE/6-31G(d) level of theory yield the closest calculated highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) energy levels of the DICTIF to the corresponding experimental ones and that TD-MPW1PW91//6-31G(d) was the most suitable level of theory for exploring electronic excitations and finding the maximum of absorption (λmax).

Polystyrene Chain Geometry Probed by Ion Mobility Mass Spectrometry and Molecular Dynamics Simulations.

Polystyrene (PS) is a thermoplastic polymer commonly used in various applications due to its bulk properties. Designing functional polystyrenes with well-defined structures for targeted applications is of significant interest due to the rigid and apolar nature of the polymer chain. Progress is hindered to date by the limitations of current analytical methods in defining the atomistic-level folding of the polymer chain. The integration of ion mobility spectrometry and molecular dynamics simulations is beneficial in addressing these challenges. However, data on gas-phase polystyrene ions are rarely reported in the literature. We herein investigate the gas phase structure of polystyrene ions with different end groups to establish how the nature and the rigidity of the monomer unit affect the charge stabilization. We find that, in contrast to polar polymers in which the charges are located deep in the ionic globules, the charges in the PS ions are rather located at the periphery of the polymer backbone, leading to singly and doubly charged PS ions adopting dense elliptic-shaped structures. Molecular dynamics (MD) simulations indicate that the folding of the PS rigid chain is controlled by phenyl ring interactions with the charge ultimately remaining excluded from the core of the globular ions, whereas the folding of polyether ions is initiated by the folding of the flexible polyether chain around the sodium ion that remains deeply enclosed in the core of the ions.

Design of self-healing and anticorrosion epoxy coating with active multiple hydrogen bonds based on grafted polyetheramine

The healing of epoxy coatings in damaged areas significantly influences their service life and protection quality. Intrinsic self-healing coatings, which enable multiple healing cycles without the need for additional functional carriers, have emerged as a promising coating technology. Nevertheless, the cross-linked structure of thermoset epoxy coating system restricts the large-scale migration of molecular chains, posing a significant challenge to achieve intrinsic healing of resin without compromising its mechanical properties. In this study, we report a design scheme for epoxy coating with intrinsic self-healing properties based on active multiple hydrogen bonds. Grafting aminobenzothiazole at the end of polyetheramine D230 chain, the grafted polyetheramine (GD230) was synthesized and served as the auxiliary curing agent, whose accurate molecular structure has been confirmed by different characterizations. The self-healing coating with reversible dynamics network based on hydrogen bonds was prepared by crosslinking through epoxy resin and mixed curing agent of polyetheramine D230 and GD230. Various measurements have been adopted to evaluate the self healing and anticorrosion performance. Results showed that the functional coating with a ratio of 8:2 for D230 to GD230 has satisfied both performances on the steel substrate. After immersion in 3 wt% NaCl solution for 120 days, the impedance value of functional coating still remains about 1010 Ω cm2. After the coating was scratched and immersion for 96 h, the impedance value of functional coating increased by about two orders of magnitude compared to that in blank coating. Mechanical testing and theoretical calculations were used to support dynamic crosslinking model to reveal the self-healing mechanism.

Digital Light Processing of Thermoresponsive Hydrogels from Polyproline-Based Star Polypeptides.

The first report of star poly(L-proline) crosslinkers is disclosed for digital light processing 3D printing of thermoresponsive hydrogels. Through chain end functionalization of star poly(L-proline)s with methacryloyl groups, access to high-resolution defined 3D hydrogel structures via digital light processing is achieved through photoinitiated free radical polymerization. Changing the poly(L-proline) molecular weight has a direct influence on both thermoresponsiveness and printability, while shape-morphing behavior can be induced thermally.

Impact of Polymer End Groups on the Formation of Solid Electrolyte Interphase at the Lithium Metal Interface: A First-Principles Calculations Study

Impact of Polymer End Groups on the Formation of Solid Electrolyte Interphase at the Lithium Metal Interface: A First-Principles Calculations Study

Solid Polymer Electrolytes with Sacrificial End Groups for a Wide Oxidative Potential and Stable Interface in Lithium Metal Batteries.

Poly(ethylene glycol) (PEG), despite being the most studied polymer electrolyte, suffers from serious drawbacks, which require fundamental studies behind its underperformance in lithium batteries. Here, we report the effect of the terminal group on triarm PEG stars bearing either hydroxyl (TPEG-OH) or carbonate-ketone (TPEG-Carb-ket) terminal groups. The latter is synthesized by a ring-opening reaction triggered by the -OH end group of TPEG-OH and results in a carbonate-ketone functionality. Indeed, the modified chain end is found to act as a sacrificial group by focusing the reactivity of the chain on the terminal group, protecting the rest of the TPEG molecule, which significantly reduces interfacial degradation and achieves a broader electrochemical stability window of up to 4.47 V, high Coulombic efficiency, and capacity retention. It furthermore demonstrates a stable interface with lithium metal after more than 1200 h of stripping and plating. When those electrolytes are investigated in reference cells based on LiFePO4 cathodes and Li anodes, the change in discharge capacity is observed from 118.7 to 113.8 and 108.9 to 5.03 mAh g-1 for TPEG-Carb-ket and TPEG-OH electrolytes, respectively, from the 1st to 100th cycle. The experimental results are further supported by density functional theory calculations and ab initio molecular dynamics simulations.

Alteration of the upper critical solution temperature (UCST) behavior of the nonionic polymer poly(N-acryloyl-nipecotamide) by its terminal group

Influences of the terminal group of non-ionic polymer with upper critical solution temperature on cloud points (CPs) has been one of curious research topics, however, detailed investigation has not been conducted so far. This study investigates the effect of the terminal group of poly(N-acryloyl-nipecotamide)s (PNANAms) on their CPs in aqueous solution. PNANAms with different molecular weights (Mn) ranging from 4,100 to 34,300 and different terminal end groups were successfully synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization. The temperature-dependent optical transmittance of aqueous solutions of the PNANAms revealed that their CP was influenced by their terminal group, in addition to their Mn and polymer concentration. PNANAms with low Mn (Mn ≥ 7,500) and a hydrophobic (dodecyl) terminal group exhibited poor solubility, whereas PNANAms with a moderate Mn (Mn ≤ 10,200) and hydrophilic (maleimide or carboxylic) terminal groups were readily soluble in aqueous solution. However, PNANAms with a high Mn (Mn = 34,300) were hardly soluble in aqueous solution. The poor solubility of such PNANAms suggests that strong inter- and intramolecular hydrogen bonds were formed among the polymer chains, increasing their CP. PNANAm with a low Mn (Mn = 4,100) and the hydrophobic terminal and that with a moderate Mn (13,200 ≤ Mn ≤ 18,200) and hydrophilic terminal groups exhibited CPs in aqueous solution. The CP of PNANAms with carboxylic terminal groups was also influenced by the solution pH owing to the deprotonation of these groups. The size of polymeric aggregates was directly observed using phase-contrast microscopy, and the temperature-dependent aggregation and dispersion of the polymers were described in terms of the inter- and intramolecular hydrogen bonds formed among PNANAm chains. Finally, a cell viability assay was conducted to explore the possibility of the applications of PNANAm in the fields of biomaterials and regenerative therapy.

Terminal α1,2-fucosylation of glycosphingolipids by FUT1 is a key regulator in early cell-fate decisions

The embryonic cell surface is rich in glycosphingolipids (GSLs), which change during differentiation. The reasons for GSL subgroup variation during early embryogenesis remain elusive. By combining genomic approaches, flow cytometry, confocal imaging, and transcriptomic data analysis, we discovered that α1,2-fucosylated GSLs control the differentiation of human pluripotent cells (hPCs) into germ layer tissues. Overexpression of α1,2-fucosylated GSLs disrupts hPC differentiation into mesodermal lineage and reduces differentiation into cardiomyocytes. Conversely, reducing α1,2-fucosylated groups promotes hPC differentiation and mesoderm commitment in response to external signals. We find that bone morphogenetic protein 4 (BMP4), a mesodermal gene inducer, suppresses α1,2-fucosylated GSL expression. Overexpression of α1,2-fucosylated GSLs impairs SMAD activation despite BMP4 presence, suggesting α-fucosyl end groups as BMP pathway regulators. Additionally, the absence of α1,2-fucosylated GSLs in early/late mesoderm and primitive streak stages in mouse embryos aligns with the hPC results. Thus, α1,2-fucosylated GSLs may regulate early cell-fate decisions and embryo development by modulating cell signaling.

Controlling the Helical Pitch of Foldamers Through Terminal Functionality: A Solid State Study.

Developing new methods to control the size and shape of the helical structures adopted by foldamers is highly important as the secondary structure displayed by these supramolecular scaffolds often dictates their activity and function. Herein, we report on a systematic study demonstrating that the helical pitch of ortho-azobenzene/2,6-pyridyldicarboamide foldamers can be readily controlled through the nature of the terminal functionality. Remarkably, simply through varying the end group of the foldamer, and without modifying any other structural features of the scaffold, the helical pitch can be over doubled in magnitude (from 3.4 Å to 7.3 Å). Additionally, crystallographic analysis of a library ten foldamers has identified general trends in the influence of a range of terminal functionalities, including carboxylbenzyl (Cbz), diphenylcarbamyl (N(Ph)2), ferrocene (Fc) and tert-butyloxycarbonyl (Boc), in controlling the folding behaviour of these supramolecular scaffolds. These studies could prove useful in the future development of functional foldamers which adopt specific sizes and shapes.

Robust Solid–Solid Phase Change Coating Encapsulated Glass Fiber Fabric with Electromagnetic Interference Shielding for Thermal Management and Message Encryption

AbstractElectromagnetic interference (EMI) shielding composites with both thermal response/management functions and message transfer/encryption behavior are ideal for use in fields such as aerospace, construction engineering, and military equipment. In this work, a self‐cross‐linking supramolecular solid–solid phase change polyethylene glycol (ScPEG) coating is prepared based on multiple hydrogen bonds, which is used for encapsulating glass fiber fabric (GFF) modified with silver nanowires (AgNWs). The solid–solid phase change coating is obtained through the hydrolysis‐condensation of PEG with a reactive silanol end group. Polyethylene glycol molecular chains can store and release heat by switching between the crystalline and amorphous state. The silanol groups can form supramolecular networks through the physical cross‐linking of multiple hydrogen bonds, resulting in an excellent thermal stability. In particular, hydrogen bonds between ScPEG and AgNW‐modified GFF (A‐GFF) enhance interfacial interactions, and the resulting robust structure enables an efficient stress transfer. ScPEG‐coated A‐GFF can achieve a tensile strength of up to 191 MPa and a tunable EMI shielding effectiveness (SE) of 40 to 72 dB depending on the number of fabric layers. Moreover, the fabric exhibits a flexible thermal response characteristic, an outstanding thermal management capability, and potential message encryption behavior.

Regulating Functional Groups to Construct a Mild Passivator Enhancing the Efficiency and Stability of Carbon-Based Perovskite Solar Cells.

The abundant defects on the perovskite surface greatly impact the efficiency improvement and long-term stability of carbon-based perovskite solar cells. Molecules with electron-donating or electron-withdrawing functional groups have been cited for passivating various defects. However, few studies have investigated the potential adverse effects arising from the synergistic interactions among functional groups. Herein, we investigate the correlation between functional group configurations and passivation strength as well as the potential adverse impacts of strong electrostatic structures by methodically designing three distinct interface molecules functionalized with different ending groups, which both belong to biguanide derivatives, including 1-(3,4-dichlorophenyl) biguanide hydrochloride (DBGCl), metformin hydrochloride (MFCl), and biguanide hydrochloride (BGCl). The results indicate that DBGCl establishes comparatively mild active sites, not only passivates defects but also aids in forming a surface with a uniform potential. Conversely, MFCl exerts a more pronounced adverse effect on the perovskite surface, which is attributable to the electronic state perturbations induced by its functional groups. Due to the lack of hydrophobic groups, devices treated with BGCl demonstrate insufficient moisture resistance. Devices passivated with DBGCl demonstrate superior average efficiency, showcasing a 12% enhancement relative to the pristine. Furthermore, DBGCl-treated devices exhibit enhanced stability in three different environments, respectively, achieving the highest PCE retention rates under nitrogen conditions (25 °C), room-temperature air conditions (25 °C, RH = 40 ± 2%), and high-temperature air conditions (65 °C, RH = 40 ± 2%).

Insights into molecular interactions at organic-MBene heterointerfaces for efficient Zn-ion storage

The intercalation of organic molecules is a promising approach to modulate the structure of 2D transition metal borides (MBenes), aiming to enhance charge transport and improve electrochemical performance in energy storage applications. However, key questions remain regarding how organic molecules with diverse functionalities penetrate and align between the MBene layer, as well as the mechanism of charge redistribution during intercalation. Addressing these questions is crucial for guiding the design of Organic-MBene heterostructures. To this end, a comprehensive approach combining theoretical calculations and experimental analyses was employed to explore the self-assembly mechanisms of organic molecules featuring N, O, S and tertiary amine end groups on the MoB-MBene surface. Experimental characterizations confirm that the interaction between MoB and organic compounds depends on the end groups. First principles calculations demonstrate that organic molecules tend to adopt a flat configuration on the MoB surface during molecular assembly. Calculations also reveal that the binding and charge transfer processes from organic molecules to MoB are highly dependent on the specific end groups, consistent with experimental observations. Furthermore, the effect of combining organic molecules with MoB on battery performance was further discussed, offering new insights for advancing the research and development of MBenes in aqueous battery systems.

A Versatile Reversible, Degenerative Chain Transfer Mechanism for the Catalytic Living Ring‐Opening Metathesis Polymerization

AbstractMost metathesis polymers based on norbornene derivatives carry a vinyl end group. Here we show that these vinyl end groups readily undergo a degenerative exchange of the terminal methylene unit in the presence of sub‐stoichiometric amounts of a propagating metathesis polymer carrying a Grubbs ruthenium complex. We show that this degenerative exchange can be exploited in synthesizing ROMP polymers in a catalytic living fashion. Chain transfer agents based on styrene, or monosubstituted conjugated 1,3 diene derivatives are used as initiating sites for the catalytic living polymerization. Suitable derivatives of these chain transfer agents not only allow the linear living growth of polymers but also the synthesis of block copolymers from macro‐initiators or star polymers from multi‐functional chain transfer agents. This reversible exchange mechanism offers a cheaper, greener, and more sustainable alternative for the synthesis of living ROMP polymers compared to the classical synthetic route.

Copper and ZnO Dual-Catalyzed Photo-Assisted Depolymerization of Poly(Methyl Methacrylate) without Deoxygenation

Despite significant advancements in thermal and photothermal depolymerizations, the success of these techniques relies on tedious deoxygenation procedures. Herein, we report the development of the depolymerization technique efficient without prior deoxygenation, which was enabled by copper/ligand complexes and the inclusion (0.25 wt% relative to solvent) of zinc oxide (ZnO) nanocrystals activated by UV light. This approach was tested for poly(methyl methacrylate) (PMMA) prepared by atom transfer radical polymerization (ATRP); the effects of solvent polarity and the activity of ligands were investigated. Unexpectedly, a low-activity Cu-complex with 2,2‘-bipyridine ligand, in combination with a low-polarity solvent, 1,2,4-trichlorobenzene, enabled relatively high depolymerization yields in less than 1 h at 150 °C. Higher activity ATRP complexes with tris(2-pyridylmethyl)amine (TPMA) and N,N,N‘,N‘‘,N‘‘-pentamethyldiethylenetriamine (PMDETA) ligands, were less efficient, which was associated with their thermal decomposition and/or the excessive formation of radicals and premature termination of the chain ends. The presented facile approach was designed to be used even in partially aerated reactors, opening new avenues for efficient depolymerization.

A Versatile Reversible, Degenerative Chain Transfer Mechanism for the Catalytic Living Ring-Opening Metathesis Polymerization.

Most metathesis polymers based on norbornene derivatives carry a vinyl end group. Here we show that these vinyl end groups readily undergo a degenerative exchange of the terminal methylene unit in the presence of sub-stoichiometric amounts of a propagating metathesis polymer carrying a Grubbs ruthenium complex. We show that this degenerative exchange can be exploited in synthesizing ROMP polymers in a catalytic living fashion. Chain transfer agents based on styrene, or monosubstituted conjugated 1,3 diene derivatives are used as initiating sites for the catalytic living polymerization. Suitable derivatives of these chain transfer agents not only allow the linear living growth of polymers but also the synthesis of block copolymers from macro-initiators or star polymers from multi-functional chain transfer agents. This reversible exchange mechanism offers a cheaper, greener, and more sustainable alternative for the synthesis of living ROMP polymers compared to the classical synthetic route.

Virtual screening of drug materials for pharmaceutical tablet manufacturability with reference to sticking

The manufacturing of pharmaceutical solid dosage forms, such as tablets involves a large number of successive processing operations including crystallisation of the drug substance, granulation, drying, milling, mixing of the formulation, and compaction. Each step is fraught with manufacturing problems. Undesired adhesion of powders to the surface of the compaction tooling, known as sticking, is a frequent and highly disruptive problem that occurs at the very end of the process chain when the tablet is formed. As an alternative to the mechanistic approaches to address sticking, we introduce two different machine learning strategies to predict sticking directly from the chemical formula of the drug substance, represented by molecular descriptors. An empirical database for sticking behaviour was developed and used to train the machine learning (ML) algorithms to predict sticking properties from molecular descriptors. The ML model has successfully classified sticking/non-sticking behaviour of powders with 100% separation. Predictions were made for materials in the handbook of Pharmaceutical Excipients and a subset of molecules included in the ChemBL database, demonstrating the potential use of machine learning approaches to screen for sticking propensity early at drug discovery and development stages. This is the first-time molecular descriptors and machine learning were used to predict and screen for sticking behaviour. The method has potential to transform the development of medicines by providing manufacturability information at drug screening stage and is potentially applicable to other manufacturing problems controlled by the chemistry of the drug substance.

CROSSLINK DENSITY AND ITS DISTRIBUTION IN HEAT AND OIL RESISTANT ELASTOMERS BY DOUBLE QUANTUM NUCLEAR MAGNETIC RESONANCE

ABSTRACT Double quantum (DQ) nuclear magnetic resonance (NMR) was used to characterize the crosslink density, crosslink density distribution, and defect level in a series of heat and oil resistant elastomers. A wide range of defect levels, crosslink densities, and crosslink density distributions was measured, and results depended on elastomer type and compound formulations, including the vulcanization system. The sol fraction defect level generally correlated with the concentration of added plasticizer in the formulation. The presence of polar side chains appeared to cause additional dynamic contributions to the dangling chain end fraction. The large differences in elastomer composition and rubber formulations prevented meaningful correlation of the measured crosslink densities with the low strain modulus. Fast Tikhonov regularization and log normalization fitting of the corrected DQ build-up curve was extremely useful to provide insight into the modality and widths of the crosslink density distributions. A high degree of heterogeneity of the crosslink network of heat and oil resistant elastomers was found. Crosslink density distributions were explained in terms of the polymer chain structure comprised of monomer sequencing coupled with the position of the crosslinking sites. The type of vulcanization system had a lesser effect of the nature of the crosslink density distribution. The primary polymer chain crosslinking sites may become segregated from the continuous phase due to polarity differences seen in the microstructure of oil and heat resistance elastomers. The development of such micromorphologies can favor curative partitioning. The sole use of DQ NMR can provide valuable insight into the nature of the polymer chain structure and crosslink network in rubber.

Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii

Natural polysaccharides named PEP-0.1-1, PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235, 2041 and 23933 Da, respectively. Further, structural characterization revealed that PEP-0.1-1 had a →4-α-D-Glcp-1→ backbone and contained →4)-α-D-Glcp and →4)-β-D-Glcp reducing end groups. PEP-0-1 backbone contained →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-1→, and the side chains contained α-D-Glcp, β-D-Manp-1→ and α-D-Glcp-3→. However, PEP-0-2 backbone consisted of →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ while the side chains contained α-D-Glcp and β-D-Manp-1→. Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4, nitric oxide, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW264.7 cells, as well as the over secretion of the above cell cytokines. Moreover, Western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of c-Jun N-terminal kinases protein protein induced by LPS in mitogen activated protein kinase pathway, along with the relieving on the inhibition effect of LPS on IκB-α protein expression. In summary, the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with anti-inflammation activities.