Endophytic Fungal Extracts Research Articles

Anti-rheumatoid Activity of Secondary Metabolites Produced by Endophytic Chaetomium globosum.

The aim of the present study was to investigate the anti-rheumatoid activity of secondary metabolites produced by endophytic mycobiota in Egypt. A total of 27 endophytic fungi were isolated from 10 dominant medicinal plant host species in Wadi Tala, Saint Katherine Protectorate, arid Sinai, Egypt. Of those taxa, seven isolates of Chaetomium globosum (CG1–CG7), being the most frequent taxon, were recovered from seven different host plants and screened for production of active anti-inflammatory metabolites. Isolates were cultivated on half – strength potato dextrose broth for 21 days at 28°C on a rotatory shaker at 180 rpm, and extracted in ethyl acetate and methanol, respectively. The probable inhibitory effects of both extracts against an adjuvant induced arthritis (AIA) rat model were examined and compared with the effects of methotrexate (MTX) as a standard disease-modifying anti-rheumatoid drug. Disease activity and mobility scoring of AIA, histopathology and transmission electron microscopy (TEM) were used to evaluate probable inhibitory roles. A significant reduction (P < 0.05) in the severity of arthritis was observed in both the methanolic extract of CG6 (MCG6) and MTX treatment groups 6 days after treatment commenced. The average arthritis score of the MCG6 treatment group was (10.7 ± 0.82) compared to (13.8 ± 0.98) in the positive control group. The mobility score of the MCG6 treatment group (1.50 ± 0.55) was significantly lower than that of the positive control group (3.33 ± 0.82). In contrast, the ethyl acetate extract of CG6 (EACG6) treatment group showed no improvements in arthritis and mobility scores in AIA model rats. Histopathology and TEM findings confirmed the observation. Isolate CG6 was subjected to sequencing for confirmation of phenotypic identification. The internal transcribed spacer (ITS) 1–5.8 s – ITS2 rDNA sequences obtained were compared with those deposited in the GenBank Database and registered with accession number KC811080 in the NCBI Database. The present study revealed that the methanol extract of endophytic fungus C. globosum (KC811080) recovered from maidenhair fern has an inhibitory effect on inflammation, histopathology and morphological features of rheumatoid arthritis in an AIA rat model.

Mycosynthesis of silver nanoparticles using extract of endophytic fungi, Penicillium species of Glycosmis mauritiana, and its antioxidant, antimicrobial, anti-inflammatory and tyrokinase inhibitory activity

Silver nanoparticles were synthesized using endophytic fungal species, Penicillium species from Glycosmis mautitiana. Phytochemicals, namely tannins, saponins, terpenoids and flavonoids, were identified in Penicillium species extracts, and act as agents of reducing and capping in the conversion of silver nanoparticles into nanoparticles. Using SEM, UV-spectroscopy and XRD, the Penicillium species silver nanoparticles (PsAgNPs) were characterized. The PsAgNPs are shown to be strong antioxidants (DDPH and FRAP), have demonstrated anti-inflammatory properties by three different methods in vitro and strongly inhibited the activity of xanthine oxidase, lipoxygenase and tyrosine kinase. E. coli and P. aeruginosa bacterial species were strongly inhibited by PsAgNPs activity at maximum levels and SEM picture of P. aeruginosa confirms these effects and that they were shrunken due to the toxic effect of PsAgNPs.

Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain.

Pasteurella multocida is an important veterinary pathogen causing infections in animals and birds. Nowadays, different reports have described the severity of infections, increasing resistance of micro-organisms to antibiotics, and the contribution of ethnoveterinary practices towards the treatment of various ailments of animals. The aim of the present study was to investigate the antibacterial efficacy of the ethanolic extracts of endophytic fungi against P. multocida Capsular Type A strains. A total of six endophytic fungi were isolated from two tropical ethnoveterinary plants: Garcinia xanthochymus H. and Polygonum chinense L. The ethanolic extracts of the endophytic fungi were subjected to in vitro antimicrobial activity by the well diffusion method. Besides, we evaluated the treatment of mice with the potent fungal extract and observed the effects in different organs under electron microscopy. Our results showed that four fungi had antimicrobial activity against the selected pathogen. The best antibacterial activity was showed by the extract of the endophytic fungi, Glomerella magna isolated from G. xanthochymus, with a minimum inhibitory concentration of 46.9 μg/mL and minimum bactericidal concentration of 750 μg/mL. Treatment of mice with the potent fungal extract caused a considerable inhibitory effect on the pathogen growth in vital organs, results that was also confirmed by histopathological studies made by scanning electron microscopy. The present findings indicated that the endophytic fungi G. magna has the potential to provide an effective treatment against infections caused by Pasteurella multocida. However, the isolation of bioactive components needs further investigation.

Spatial and seasonal influences on culturable endophytic mycobiota associated with different tissues of Eugenia jambolana Lam. and their antibacterial activity against MDR strains.

BackgroundPresent study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of Eugenia jambolana. The influence of season and geographical location on diversity and distribution of endophytic fungi has been analyzed. Antibacterial activity of isolated fungal species has also been investigated against MDR bacterial strains.ResultA total of 1896 endophytic fungal isolates were obtained from healthy, surface sterilized tissues of leaf, stem and petiole tissues during summer, monsoon and winter season. Out of 24 fungal species isolated, 20 species belong to class Ascomycetes, 2 to Basidiomycetes and 2 to Zygomycetes. Maximum species diversity was in rainy season whereas colonization frequency was in winter. All the diversity indices showed maximum species diversity at site 5 (Yamunanager), rainy among the seasons and leaf among the tissues studied. Aspergillus genus was most frequently isolated. Aspergillus niger and Alternaria alternata were most dominant species. Three way ANOVA results showed that effect of season was highly significant on species diversity in relation to sites and tissues. 60 % endophytic fungal extracts showed significant antibacterial activity against one or more than one MDR bacterial strain.ConclusionDifferent fungal species were recovered from different sites but the inter-site comparisons were not significant according to Jaccard similarity coefficient. Diversity of such fungal endophytes indicates that Eugenia jambolana plant acts as an ecosystem facilitating survival of many microbes with impressive antibacterial potential.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0664-0) contains supplementary material, which is available to authorized users.

Aktivitas Antibakteri Kapang Endofit dari Tanaman Kina (Cinchona calisaya Wedd.)

Endophytic microorganisms are microorganisms that live in the tissues of plant organ and not harm its host. One of them is the endophytic fungi. Endophytic fungi could produce the same compound as the host plant. The Plants produced alkaloids quinine that could potentially inhibit the bacteria Staphylococcus aureus and Escherichia coli. This study aims to test the potential of endophytic fungi produced quinine as an antibacterial. The method used to test the antibacterial is a paper disc diffusion. Results of the analysis of data using one-way ANOVA showed that there are significant differences zona between the diametre of inhibitory of the endophytic fungi extract. Endophytic fungi M16 and M33 produced the greatest inhibition zone against Staphylococcus aureus and Escherichia coli 14.9 mm and 9.2 mm respectively.

Test Antifungal Phatogen Extract Secondary Metabolites Of Endophytic Fungi Raru Plant (Cotylelobium melanoxylon)

This study aims to determine the ability of extracts secondary metabolites of endophytic fungi raru plant Siarang (Cotylelobium melanoxylon) in inhibiting the growth of pathogenic fungi. Pathogenic fungi tested were Collectotrichum, Fusarium oxysporum, Candida albicans and Sclerotium rolfsii. Test antifungal pathogens carried out by using the method of Kirby-Bour, ie by measuring the clear zone located around the paper disc which is the zone of growth inhibition of pathogenic fungi. Measurement of inhibition zone is done by using a caliper or ruler. The results showed that the secondary metabolites of endophytic fungi extracts could inhibit the growth of pathogenic fungus Candida albicans is the clear zone of 10.23 mm. Keywords : endophytic fungus, Cotylelobium melanoxylon, extract of secondary metabolites, fungal pathogens, inhibition zone

Bioprospecting Endophytic Fungi and Their Metabolites from Medicinal Tree Aegle marmelos in Western Ghats, India

The increasing emergence of lead drugs for the resistance produced by the pathogenic strains and arrival of new diseases have initiated the need for searching novel metabolites with best anticancer and antimicrobial properties than the existing one. With this view, the investigation was conducted for the isolation, identification, and biological evaluation of potential endophytic fungi of Aegle marmelos, a medicinal tree used for more than three decades, for curing various disorders. A total of 169 endophytic fungal strains obtained from sampling and among those 67 were pigmented strains. Upon antagonistic screening, five endophytic fungal strains exhibited antagonistic potentiality by inhibiting the pathogens. These five potent strains were characterized at molecular level by sequencing the amplified internal transcribed spacer (ITS) 1 and ITS 4 regions of rDNA and they were grouped under order Pleosporales, Eurotiales, and Capnodiales. The metabolites from the respective strains were produced in fungal culturing media and extracted using polar solvents. Further, the extracts of five endophytes manifested antimicrobial activity against tested clinical pathogens and Alternaria alternata (FC39BY), Al. citrimacularis (FC8ABr), and Curvularia australiensis (FC2AP) exhibited significant antimicrobial profile against 9 of 12 tested pathogens, showing broad spectrum activity. The antioxidant levels of all the five endophytes revealed the highest activity at least concentrations, and major activity was unveiled by the members of order Pleosporales FC2AP and FC8ABr. This research explains the value of endophytic fungal extracts and its significance of antimicrobial and antioxidant properties.

A pilot study of antioxidant potential of endophytic fungi from some Sudanese medicinal plants.

To evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five Sudanese medicinal plants: Calotropis procera, Catharanthus roseus, Euphorbia prostrate, Vernonia amygdalina and Trigonella foenum-graecum. Crude extracts of endophytic fungi and their host plants were tested by classical Folin-Ciocalteu colorimetric method to determine the total phenolic content, also total antioxidant capacity was estimated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging invitro method. Among the endophytes, endophytic fungus Aspergillus sp. from Trigonella foenum-graecum seeds demonstrated the highest both total phenolic content in term of gallic acid equivalent [(89.9±7.1) mg GAE/g] and antioxidant activity for 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay [IC50: (18.0±0.1) μg/mL]. A high positive linear correlation (R(2)=0.999 1) was found between total antioxidant capacity and total phenolic content of endophytic fungi isolated from Vernonia amygdalina. The present study revealed that some endophytic fungi from the five Sudanese medicinal plants could be a potential source of novel natural antioxidant compounds.

Larvicidal activity of endophytic fungal extract of Cochliobolus spicifer (Pleosporales: Pleosporaceae) on Aedes caspius and Culex pipiens (Diptera: Culicidae)

Endophytic microorganisms are fungi or bacteria which live inside plant tissues or organs, without causing them any harmful symptoms. Indeed, since many are also able to produce substances of biotechnological interest, they may protect the plant from insect attacks and diseases. The date palm, Phoenix dactylifera (Linnaeus) is a tree which has been known in Saudi Arabia for thousands of years as a staple food. The aim of the present work was to investigate the inhibitory activity of crude extracts of the fungal endophyte Cochliobolus spicifer (Nelson), isolated from the date palm, against Aedes caspius (Pallas) and Culex pipiens (Linnaeus) larvae. In addition, histological changes were determined in the digestive system of treated third instar larvae. The data showed that C. spicifer interfered with the developmental cycle of the mosquito, and had its strongest toxic effect (with 100 % mortality in third instar larvae) at concentrations of 300 ppm. This compound did not show toxicity to zebrafish. The third instar larvae treated with the active fraction showed cellular destruction and disorganization, cell spacing, and vacuolization of epithelial cells in small regions of the midgut. To the authors’ knowledge, this is the first study to report the isolation of endophytic fungi C. spicifer from date palm trees (P. dactylifera). The endophytic fungal extract of C. spicifer proved to be a strong candidate for a natural, safe, and stable phytolarvicidal to be used in population control of Ae. caspius and Cx. pipiens.

Isolation and Evaluation of Antimicrobial Activities of Endophytic Fungal Extract from Mallotus philippinensis Muell

Mallotus philippinensis Muell an important medicinal plant in the present study i.e., endophytic extracts of different fungi associated with asymptomatic were evaluated for antimicrobial activity. This will be a new promising source of antimicrobials known as endophytes. In this preliminary study, different parts of plants viz. steam and leaves were used for isolation of endophytic fungi and fermented. The endophytic fungi were identified based on their morphology and characteristics of fungal spores. The cell free ferment broth was subjected to antimicrobial assay against different human pathogenic microbes viz. Escherichia coli, Staphylococcus aureus, E. faecalis, K .pneumoniae and P. aeruginosa and three fungal pathogenic strains of candida i.e. C. albicans, C. tropicalis and C. krusie by using standard disc diffusion method. The results showed that some of these endophytic extracts had prominent antimicrobial inhibitory effects. Among these fungal endophytes, two strains Alternaria spp., Pestalotiopsis spp., and Phompsis spp. showed the strongest antimicrobial activities against P. aeruginosa, S. aureus and E. faecalis. An endophyte Pestalotiopsis spp. had the most pronounced effect on all the Candida strains exhibited the strongest antifungal activity. This present study has proven that both these medicinal plants may be a rich source of endophytic fungi with potential to produce bioactive producing antibacterial and antifungal activities. These antipathogenic endophytes could be applied as new sources of antibiotics in agriculture and pharmaceutical industry.

Ultrastructural effect on mastitis pathogens by extract of endophytic fungi associated with ethnoveterinary plant, Hibiscus sabdariffa L.

Three endophytic fungi isolated from different parts of Hibiscus sabdariffa L. were identified using morphological and molecular approaches. Ethanolic extract of endophytic fungi as well as plant extracts were evaluated for in vitro antibacterial activity by using well diffusion method and their minimum inhibitory concentration estimated. The culture extract of one endophytic fungus Glomerella acutata EF15 was found to be potent antibacterial agent against pathogenic coliform bacteria Klebsiella pneumoniae and Escherichia coli responsible for causing clinical mastitis. Scanning electron microscopy (SEM) revealed the ultrastructural alteration in the cells of K. pneumoniae and E. coli when treated with crude ethanolic extract of G. acutata. The ethanolic extract of the endophytic fungus revealed potential to be bioprospected as antibacterial agent against pathogens causing coliform mastitis, the existing havoc of dairy industries.

In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam.

ObjectiveTo elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays. MethodsTwenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity. ResultsAlkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential. ConclusionsThe results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.

Safety of Malaysian marine endophytic fungal extract S2 from a brown seaweed Turbinaria conoides

Objective: To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides. Methods: Two doses (100 mg/kg and 400 mg/kg) of the S2 extract were administered to rats orally for acute toxicity and antioxidant test. The body weight, relative weight of six organs, haematological, biochemical and antioxidant properties were investigated on Day 14. Results: A single oral dose treatment did not cause any mortality or observable adverse effects in rats. No significant variations in the body and organ weights between the control and the treated groups were observed. Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract. The total white blood cell count and haemoglobin levels were increased. The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly (P<0.05) decreased (1.28 and 1.34 mmol/L respectively) compared to control (1.55 mmol/L) rats. Pathologically, neither gross abnormalities nor histopathological changes were observed. This study showed strong evidence of the non-toxic effects of S2 extract. Furthermore the extract exhibited significant (P<0.05) antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzymes in serum, liver and kidney. Conclusions: The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds. Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe, thus deserving further extensive investigation.

DETEKSI SENYAWA METABOLIT SEKUNDER JAMUR ENDOFIT DARI AKAR TANAMAN KARET (Hevea brasiliensis)

Endophytic fungi are a group of fungi whose part or whole life is in living plant tissue and usually does not harm the host. Endophytic fungi generally produce secondary metabolites that have beneficial biological activities such as anti-cancer compounds, antifungi or antibacterials. Control of plant diseases caused by Rigidoporus microporus and Ganoderma boninense can be done by using biological agents, namely by using endophytic fungi. This fungus has metabolite compounds that can inhibit the growth of Rigidoporus microporus and Ganoderma boninense. This study aims to determine what content is found in endophytic mushroom extracts isolated from rubber plants (Hevea brasiliensis). Endophytic fungi are extracted by maceration using methanol and ethyl acetate solvents. The extract was tested for antifungal activity using diffusion method. The content of secondary metabolites was tested by phytochemical test. The results of endophytic fungal extraction with methanol and ethyl acetate solvents resulted in antifungal activity with inhibitory zones in a row namely WL01: 2.19 and 2.15 mm Fungi Isolates on Ganoderma boninense while 3.09 mm WL02 against Rigidoporus microporus Based on the phytochemical test results, endophytic fungal extract with methanol and ethyl acetate solvents showed the presence of alkaloid, flavonoid, phenol and saponin. Keywords: endofit, fungi, hevea, secondary metabolites

Bioactivity assessment of endophytic fungi associated with Centella asiatica and Murraya koengii

Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. LeavesMbouobda Hermann Desire , Fotso Bernard , Muyang Rosaline Forsah , Chiatoh Thaddeus Assang, Omokolo Ndoumou Denis

Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.

Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana.

The aim of this study was to identify the endophytic fungi of the medicinal plant Kigelia africana (Lam) Benth. (Bignoniaceae) and to investigate their potential antimicrobial activity. Seven species of endophytic fungi were successfully isolated from K. Africana for the first time: including Cladosporium sp., Aspergillus flavus, Aspergillus sp., Curvularia lunata as well as three unknown species. The fungal extracts were assessed for antibacterial activity against three standard pathogenic bacterial strains: Bacillus subtilis, Staphylococcus aureus and Escherichia coli. Most of the extracts showed in vitro inhibition of bacterial growth. The phytochemical screening revealed the existence of a diverse group of secondary metabolites in the crude extracts of the endophytic fungi that resemble those in the host plant extracts.

Biological Activity of Endophytic Fungi of Rauwolfia serpentina Benth: An Ethnomedicinal Plant Used in Folk Medicines in Northeast India

Rauwolfia serpentina Benth is an ethnomedicinal plant used as anti-hypertensive remedy by the ethnic tribes of northeast India. The plant was screened for the endophytic fungi and their associated biological activities to assess if these fungi have any significant hypocholesterolemic, anti-oxidant and antimicrobial activities. The endophytes were characterized as Colletotrichum gloeosporioides, Penicillium sp. and Aspergillus awamori, from a total 15 isolates, using biochemical and molecular approaches. The colonization rate of endophytes in the leaf and the stem was found to be 12.04 and 4.02 % respectively. C. gloeosporioides was found to be the dominant fungus with an average colonization frequency of 6.8 % followed by Penicillium sp. (3.8 %) and A. awamori (2.1 %). Being the most potential isolate, Penicillium sp. showed the promising hypocholesterolemic activity. The antimicrobial potential against four pathogenic bacteria, viz., Streptococcus pyogenes, Escherichia coli, Enterococcus faecalis, Salmonella enterica ser paratyphi and three fungal pathogens, viz, Candida albicans, Emericella nidulans var nidulans, Fusarium oxysporum showed promising antagonistic ability. A. awamori showed the highest antioxidant potential and also had the maximum phenolic content among the characterized endophytes. This investigation revealed that the endophytic fungal extracts can be a potential source of alternative natural hypocholesterolemic, antimicrobials and antioxidant agents mimicking the activity of the host plant and purification of active metabolites may provide lead molecule of pharmaceutical importance.

Endophytic fungi isolated from oil-seed crop Jatropha curcas produces oil and exhibit antifungal activity.

Jatropha curcas L., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. The plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. The plant was explored for its endophytic fungi for use in crop protection. Endophytic fungi were isolated from leaf of Jatropha curcas, collected from New Delhi, India. Four isolates were identified as Colletotrichum truncatum, and other isolates were identified as Nigrospora oryzae, Fusarium proliferatum, Guignardia cammillae, Alternaria destruens, and Chaetomium sp. Dual plate culture bioassays and bioactivity assays of solvent extracts of fungal mycelia showed that isolates of Colletotrichum truncatum were effective against plant pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. Isolate EF13 had highest activity against S. sclerotiorum. Extracts of active endophytic fungi were prepared and tested against S. sclerotiorum. Ethyl acetate and methanol extract of C. truncatum EF10 showed 71.7% and 70% growth inhibition, respectively. Hexane extracts of C. truncatum isolates EF9, EF10, and EF13 yielded oil and the oil from EF10 was similar to oil of the host plant, i.e., J. curcas.

Antimicrobial Activity of Crude Extracts of Endophytic Fungi Isolated from Medicinal Plant Sapindus saponaria L.

Article history: Received on: 07/09/2012 Revised on: 19/09/2012 Accepted on: 24/09/2012 Available online: 28/10/2012 Endophytic microorganisms are fungi or bacteria which live inside plant tissues or organs, without causing them any harmful symptoms. They may protect the plant from insect attacks and diseases, being also able to produce substances of biotechnological interest. Sapindus saponaria L. is a tree commonly known in Brazil as “sabaode-soldado”. In folk medicine, its bark, root and fruit are used as producing anxiolytic, astringent, diuretic and expectorant substance, as well as tonic, blood depurative and cough medicine. Its leaves extracts present properties that neutralize bleeding. The fruit extract presents antifungical and larvicidal activities. The aim of the present work was to investigate biotechnological potential of crude extracts of fungal endophytes (G2-20 Cochliobolus intermedius, G1-74 non-identified - NI, G22-97 Phomopsis sp. and G23-100 NI) isolated from S. saponaria, that have been assayed against five pathogenic bacteria.The antibacterial activities with extracts obtained from the four endophytic lineages were promising, since all of them inhibited the growth of at least one of the tested bacteria. One metabolite, extracted from the lineage G2-20 (Cochliobolus intermedius) presented activity for all the bacteria tested. The results showed that S. saponaria isolates presented biotechnological potential for the control of pathogenic bacteria tested in vitro.