Encapsulation Stability Research Articles

Effects of calcium ions on the particle performance of luteolin-loaded zein-gum arabic-tea polyphenols ternary complex nanoparticles

Zein-gum arabic-tea polyphenols ternary complex nanoparticles have been developed for the encapsulation of luteolin, but there are still shortages existed in their particle properties such as encapsulation efficiency, stability, and release ability of luteolin. To improve their performance, calcium ions (Ca2+) were employed as a cross-linking stabilizer to investigate their effects on particle properties and functional performance. Particle parameters, physicochemical stability, antioxidant activity, and in vitro simulated gastrointestinal digestion were evaluated based on zetasizer measurement and multi-spectral methods, which were analyzed using Graphpad Prism v6.0.2. As a result, low concentrations of Ca2+ induced the formation of smaller and more compact nanoparticles with improved luteolin encapsulation efficiency, while higher concentrations had the opposite effect. Hydrophobic, electrostatic, and hydrogen bonding interactions were found to be responsible for nanoparticle assembly and stability with Ca2+ incorporation. Appropriate amounts of Ca2+ promoted the physicochemical stability of the nanoparticles under different conditions such as pH, salt ion concentration, temperature, and storage time. Additionally, low levels of Ca2+ strengthened the antioxidant activity of the nanoparticles and improved sustained release of luteolin under in vitro gastrointestinal conditions. These findings suggest that zein-gum arabic-tea polyphenols nanoparticles assembled with Ca2+ have potential applications as a delivery system for bioactive substances.

Fabrication of natural W1/O/W2 double emulsions stabilized with gliadin colloid particles and soybean lecithin

In this study, we successfully fabricated W1/O/W2 double emulsions and high internal phase double emulsions with gliadin colloid particles (GCPs) as the hydrophilic emulsifier and soybean lecithin as the hydrophobic emulsifier, which were obtained from natural resources and avoided the use of surfactants such as PGPR. The effects of lecithin concentration, GCP concentration, W1/O volume ratio, and pH of the external aqueous phase on the physical properties and encapsulation efficiency of the double emulsions were investigated. The double emulsions presented an encapsulation efficiency (tartrazine was used as an aqueous marker) up to 91.1 ± 0.2% under the optimised conditions (GCP concentration 2% (w/v), lecithin concentration 1% (w/v), pH 6 of the W2 phase, W1/O volume ratio 1:5). After 30-days of storage at room temperature, the encapsulation efficiency of the double emulsions remained stable at 88.8 ± 0.2%, indicating good encapsulation stability. The double emulsions exhibited pH response characteristics; their appearance changed from a semi-solid to a flow state as the pH of the W2 phase increased, and the formation of the gel net structure facilitated the maintenance of the internal aqueous phase during fabrication and storage. This study provides a strategy for the fabrication of natural double emulsions with satisfactory encapsulation efficiency.

Co-encapsulation of curcumin and quercetin with zein/HP-β-CD conjugates to enhance environmental resistance and antioxidant activity

In this study, composite nanoparticles consisting of zein and hydroxypropyl beta-cyclodextrin were prepared using a combined antisolvent co-precipitation/electrostatic interaction method. The effects of calcium ion concentration on the stability of the composite nanoparticles containing both curcumin and quercetin were investigated. Moreover, the stability and bioactivity of the quercetin and curcumin were characterized before and after encapsulation. Fluorescence spectroscopy, Fourier Transform infrared spectroscopy, and X-ray diffraction analyses indicated that electrostatic interactions, hydrogen bonding, and hydrophobic interactions were the main driving forces for the formation of the composite nanoparticles. The addition of calcium ions promoted crosslinking of the proteins and affected the stability of the protein–cyclodextrin composite particles through electrostatic screening and binding effects. The addition of calcium ions to the composite particles improved the encapsulation efficiency, antioxidant activity, and stability of the curcumin and quercetin. However, there was an optimum calcium ion concentration (2.0 mM) that provided the best encapsulation and protective effects on the nutraceuticals. The calcium crosslinked composite particles were shown to maintain good stability under different pH and simulated gastrointestinal digestion conditions. These results suggest that zein–cyclodextrin composite nanoparticles may be useful plant-based colloidal delivery systems for hydrophobic bio-active agents.

Optimization of solvent evaporation method in liposomal nanocarriers loaded-garlic essential oil (Allium sativum): Based on the encapsulation efficiency, antioxidant capacity, and instability.

This study is aimed to optimise the preparation factors, such as sonication time (5-20min), cholesterol to lecetin ratio (CHLR) (0.2-0.8), and essential oil content (0.1-0.3g/100g) in solvent evaporation method for formulation of liposomal nanocarriers containing garlic essential oil (GEO) in order to find the highest encapsulation efficiency and stability with strongest antioxidant and antimicrobial activity. The droplet size, zeta potential, encapsulation efficiency, turbidity, changes in turbidity after storage (as a measure of instability), antioxidant capacity, and antimicrobial activity were measured for all prepared samples of nanoliposome. The sonication time is recognised as the most effective factor on the droplet size, zeta potential, encapsulation efficiency, turbidity, and instability while CHLR was the most effective factor on zeta potential and instability. The content of GEO significantly affected the antioxidant and antimicrobial activity in particular against gram-negative bacteria (Escherichia coli). The results of FTIR based on the identification of functional groups confirmed the presence of GEO in the spectra of the prepared nanoliposome and also it was not observed the interaction between the components of the nanoliposome. The overall optimum conditions were determined by response surface methodology (RSM) as the predicted values of the studied factors (sonication time: 18.99min, CHLR: 0.59 and content of GEO: 0.3g/100g) based on obtaining the highest stability and efficiency as well as strongest antioxidant and antimicrobial activity.

Radiopharmaceutical Encapsulated Liposomes as a Novel Radiotracer Im - aging and Drug Delivery Protocol.

Data are presented for 5 male New Zealand white rabbits. They were injected intravenously with the 99mTc-liposomes encapsulated MIBI through a marginal ear vein, and whole-body images were acquired using a dual-head gamma camera. Cationic PEGylated liposomes were prepared using the conventional thin-film-hydration method. The liposomes were tested for particle size, zeta potential, high-performance-liquid-chromatography (HPLC), and toxicity. The liver activity was slightly greater than or equivalent to heart uptake, using 99mTcsestamibi, MIBI, without liposome as a reference. The absorbed doses in myocardium cells after injecting rabbits with 99mTc-MIBI labeled with free positive lower pH liposomes was greater than in the liver, whereas 99mTc labeled with encapsulated MIBI within positive liposomes showed a significantly higher heart-to-liver ratio. The heart-to-spleen activity uptake ratio in 99mTc-MIBI was higher than or equal to one but increased in 99mTc labeled with MIBI and free positive liposomes. Injecting rabbits with 99mTc labeled with encapsulated MIBI raised myocardium uptake to 2-4 times more than the spleen. Heart-to-bowel activity began to rise with 99m Tc-labeld-MIBI and liposomes. This study provides findings in radiopharmaceutical biodistribution using liposomal agents. Adding free liposomes using a pH gradient technique enhanced the uptake and localization of the radiotracer. However, tracer encapsulation during the formation of the liposomes showed even better specificity.

Formulation and Characteristics of Nanostuctured Lipid Carrier (NLC) Red Palm Oil (RPO) Prepared by High-Pressure Homogenization and Its Applications in Orange Juice

A Nanostructured lipid carrier (NLC) is an emulsion system that can encapsulate and improve stability of lipid-based bioactive compounds. This study aimed to develop an optimal RPO-NLC formula with a combination of solid and liquid lipids, lipids and surfactants, and the use of high-pressure homogenizer (HPH). RPO-NLC was made using HPH with a 400 bar and 600 bar pressure. A Completely randomized design (CRD) with three factors was used to determine interactions between factors. The RPO-NLC formula was characterized based on particle size. One of the best formulas was selected with small particle size and high RPO content then characterized based on encapsulation efficiency (EE), loading capacity (LC), storage stability, FTIR, thermal and applied to orange juice including sensory evaluation, encapsulation stability and pH. RPO-NLC yielded particle size 44.9 nm, EE 99.9±0.02%, LC 4.9±0.001%, was stable on storage. β-carotene identified in RPO-NLC lipid matrix based on FTIR analysis and has good thermal stability based on differential scanning calorimeter analysis. Sensory evaluation showed changes in sensory attributes of color, aroma, taste during storage. Encapsulation stability showed significant increase during storage and pH measurement results also increased.

Encapsulation of Silicon Nano Powders via Electrospinning as Lithium Ion Battery Anode Materials.

Silicon-containing polyester from tetramethoxysilane, ethylene glycol, and o-Phthalic anhydride were used as encapsulating materials for silicon nano powders (SiNP) via electrospinning, with Polyacrylonitrile (PAN) as spinning additives. In the correct quantities, SiNP could be well encapsulated in nano fibers (200-400 nm) using scanning electron microscopy (SEM). The encapsulating materials were then carbonized to a Si-O-C material at 755 °C (Si@C-SiNF-5 and Si@C-SiNF-10, with different SiNP content). Fiber structure and SiNP crystalline structure were reserved even after high-temperature treatment, as SEM and X-ray diffraction (XRD) verified. When used as lithium ion battery (LIB) anode materials, the cycling stability of SiNPs increased after encapsulation. The capacity of SiNPs decreased to ~10 mAh/g within 30 cycles, while those from Si@C-SiNF-5 and Si@C-SiNF-10 remained over 500 mAh/g at the 30th cycle. We also found that adequate SiNP content is necessary for good encapsulation and better cycling stability. In the anode from Si@C-SiNF-10 in which SiNPs were not well encapsulated, fibers were broken and pulverized as SEM confirmed; thus, its cycling stability is poorer than that from Si@C-SiNF-5.

A non-thermal modification method to enhance the encapsulation efficiency, stability, and slow-release performance of zein-based delivery systems – Cold plasma

Zein is widely used as a nutrient/drug delivery carrier due to its self-assembling property. However, the low encapsulation efficiency (EE) and stability limit its applications. In this study, zein nanoparticles are modified by cold plasma technology. The characterization results show that the modified zein exhibits more uniformly dispersed spheres with a more ordered secondary structure (increased α-helix, β-turn, and reduced β-sheet), but the molecular weight is not affected. The modified nanoparticles (85 W, 2 min) have a smaller particle size (100.03 nm) and a larger ζ-potential (41.07 mV). When used as delivery wall material, their stronger self-assembling dynamics and more binding sites enhance the interactions between zein and curcumin. This causes an enhancement in the curcumin burst release property, EE (52.83%–76.31%), pH, ionic strength, and storage stability. Therefore, cold plasma is an effective modification method to improve the EE, stability, and slow-release properties of zein-based delivery systems.

Formulation and Characteristics of Red Palm Oil Nanostractured Lipid Carriers Prepared by Microemulsion Method and Its Application in Drinking Yoghurt

The aim of this study was to investigate the characteristic of red palm oil nanostructured lipid carriers (RPO-NLC) made with microemulsion method. The microemulsion procedures prepared with ratio Tween 80 : Span 20 (80:20 ; 70:30), lipid : surfactant (1:3.75 ; 1:4), red palm oil : palm stearin (8:2 ; 7:3 ; 6:4). The best particle size was found at ratio 80:20, 1:4, 6:4 respectively with 24.73 ± 0.06 nm, zeta potential -7.43, turbidity 0,183% and polydispersity index at 0.06. This formulation indicated a loading capacity of 1.97%, encapsulation efficiency of 99.57%, FTIR, DSC, and stable emulsion at room temperature storage for four weeks. This best formulation was applied in drinking yoghurt with ratio nanostructured lipid carrier and yoghurt 1:18. The pH of yoghurt drink containing RPO-NLC increased from 4.13 to 4.37, while its encapsulation stability decreased from 78.45% to 49.29%. The results of sensory evaluation showed no significant difference in color, aroma, but there was significant difference on the taste of drinking yoghurt.

Encapsulation of resveratrol in rhamnolipid-zein nanoparticles using a pH-driven method: kinetic modeling on controlled release from nanoparticles

In this study, the encapsulation of resveratrol (Res) in zein-rhamnolipid (RL) composite nanoparticles (NPs) by a pH-driven method and its relationship with the controlled release behavior of the NPs were investigated. The properties of the produced NPs were elucidated with different zein-RL mass ratios and encapsulation of different amounts of Res. The NPs had relatively high encapsulation efficiency, loading capacity, stability, and cumulative release percentages. The SEM analysis showed the spherical NPs obtained with and without Res incorporation and the FTIR study indicated the interactions of Res with zein-RL components. The NPs had relatively good stability over a wide range of pH (4–9), salt concentration (0–200 mM) (pH = 7), and Res amount (0–160 mg) (pH = 7.4). Ultrasonic and shaking water baths were used for release studies of Res from NPs with an optimum zein-RL mass ratio of 1.0:0.6 (w/w). In vitro release studies of Res under acidic (pH = 1.2) and neutral (pH = 7.4) conditions demonstrated initial rapid release followed by slow release. The highest cumulative release percentage was obtained at pH 1.2 and 7.4 with 120 mg Res encapsulated zein-RL NPs. The release of Res from the capsules was a pH-dependent process and the cumulative release profiles were considered to be slightly higher in acidic environments. Among the controlled release kinetic models, the Weibull model gave the best fit for optimum Res-loaded NPs. Release studies indicated that zein-RL NPs have the potential to be used as delivery systems for health-promoting bioactive molecules in functional foods, nutraceutical/dietary supplements, and pharmaceutical formulations.

Valorization of plum (Prunus domestica) peels: microwave-assisted extraction, encapsulation and storage stability of its phenolic extract

Valorization of plum (Prunus domestica) peels: microwave-assisted extraction, encapsulation and storage stability of its phenolic extract

Encapsulation of ascorbyl palmitate in maize granular starch through an irradiation–hydrothermal method

In this study, maize starch treated with γ radiation (0–10 kGy) was used to prepare maize granular starch-ascorbyl palmitate (MGS-AP) complexes by a hydrothermal method. The MGS-AP complex encapsulation efficiency and physicochemical properties were evaluated. The irradiation effect on starch chain degradation increased the percentage of ascorbyl palmitate (AP) encapsulated in the starch and considerably raised the Rapid Visco Analyzer (RVA) profile viscosity in the later cooling stage. With increasing irradiation dose, the MGS-AP complex swelling power, relative crystallinity and thermal stability gradually increased, and the antioxidation activity first decreased and then increased, with a minimum value at 1 kGy. Generally, the MGS-AP complexes had relatively high encapsulation efficiencies, and a complex with the highest swelling power, crystallinity, shearing resistance, antioxidation activity and thermal stability was obtained with 10 kGy irradiation. This study provides a basis for preparing granular starch-AP complexes with a relatively high encapsulation efficiency and stability.

Fabrication of grape seed proanthocyanidin-loaded W/O/W emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate with konjac glucomannan: Structure, stability, and in vitro digestion

In this work, the effects of konjac glucomannan (KGM) concentrations on microstructure, gel properties, stability and digestibility of water-in-oil-in-water emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate were investigated. Visual appearance indicated that a non-layered double emulsion gel was formed when KGM increased to 0.75%. Emulsion gels with 1.5% KGM showed the highest encapsulation, freeze-thaw and photochemical stability due to the formation of the smallest droplets, which were supported by microscopic observations. Moreover, the addition of KGM improved water holding capacity, rheological and texture properties of emulsion gels. Particularly, at 1.5% or 1.75% KGM, color and potential of hydrogen showed the most stable level after 14 days of storage. During in vitro digestion, KGM delayed the hydrolysis of protein and oil droplets, and then improved the bioavailability of grape seed proanthocyanidin. These results promoted the application of KGM in emulsion gels and the encapsulation of nutraceuticals.

Niosomes for Topical Application of Antioxidant Molecules: Design and In Vitro Behavior

In the present study, gels based on xanthan gum and poloxamer 407 have been developed and characterized in order to convey natural antioxidant molecules included in niosomes. Specifically, the studies were conducted to evaluate how the vesicular systems affect the release of the active ingredient and which formulation is most suitable for cutaneous application. Niosomes, composed of Span 20 or Tween 20, were produced through the direct hydration method, and therefore, borate buffer or a micellar solution of poloxamer 188 was used as the aqueous phase. The niosomes were firstly characterized in terms of morphology, dimensional and encapsulation stability. Afterwards, gels based on poloxamer 407 or xanthan gum were compared in terms of spreadability and adhesiveness. It was found to have greater spreadability for gels based on poloxamer 407 and 100% adhesiveness for those based on xanthan gum. The in vitro diffusion of drugs studied using Franz cells associated with membranes of mixed cellulose esters showed that the use of a poloxamer micellar hydration phase determined a lower release as well as the use of Span 20. The thickened niosomes ensured controlled diffusion of the antioxidant molecules. Lastly, the in vivo irritation test confirmed the safeness of niosomal gels after cutaneous application.

Tuning egg yolk granules/sodium alginate emulsion gel structure to enhance β-carotene stability and in vitro digestion property

In this study, emulsion gels were constructed by ionic gelation method using egg yolk granules/sodium alginate bilayers emulsion. In particular, the main driving force of the emulsion gels was controlled by adjusting pH. Compared with pH 7.0, the mechanical properties of EYGs emulsion gel were enhanced at pH 4.0 (G′ > G″). The interfacial protein aggregation that occurred at pH 4.0 promoted the compactness of the EYGs emulsion gel structure along with enhanced capillary effect. The emulsion gel structure tended to be complete at 1 % SA of pH 4.0, for the electrostatic interaction required more SA molecules involved in maintaining emulsion gel structural stability. The denser emulsion gel structure of pH 4.0 than pH 7.0 improved storage stability, FFA releasing, and chemical stability of β-carotenes. Bioaccessibility of β-carotenes also decreased to achieve sustained release. This study provides a theoretical basis for tuning emulsion gel structure to adjust encapsulation stability and in vitro digestion characteristics of active ingredients.

Bioderived Lipoic Acid-Based Dynamic Covalent Nanonetworks of Poly(disulfide)s: Enhanced Encapsulation Stability and Cancer Cell-Selective Delivery of Drugs.

Dynamic covalent poly(disulfide)-based cross-linked nanoaggregates, termed nanonetworks (NNs), endowed with pH- and redox-responsive degradation features have been fabricated for stable noncovalent encapsulation and triggered cargo release in a controlled fashion. A bioderived lipoic acid-based Gemini surfactant-like amphiphilic molecule was synthesized for the preparation of nanoaggregates. It self-assembles by a entropy-driven self-assembly process in aqueous milieu. To further stabilize the self-assembled nanostructure, the core was cross-linked by ring-opening disulfide exchange polymerization (RODEP) of 1,2-dithiolane rings situated inside the core of the nanoaggregates. The cross-linked nanoaggregates, i.e., nanonetwork, are found to be stable in the presence of blood serum, and also, they maintain the self-assembled structure even below the critical aggregation concentration (CAC) as probed by dynamic light scattering (DLS) experiments. The nanonetwork showed almost 50% reduction in guest leakage compared to that of the nanoaggregates as shown by the release profile in the absence of stimuli, suggesting high encapsulation stability as evidenced by the fluorescence resonance energy transfer (FRET) experiment. The decross-linking of the nanonetwork occurs in response to redox and pH stimuli due to disulfide reduction and β-thioester hydrolysis, respectively, thus empowering disassembly-mediated controlled cargo release up to ∼87% for 55 h of incubation. The biological evaluation of the doxorubicin (DOX)-loaded nanonetwork revealed environment-specific surface charge modulation-mediated cancer cell-selective cellular uptake and cytotoxicity. The benign nature of the nanonetwork toward normal cells makes the system very promising in targeted drug delivery applications. Thus, the ease of synthesis, nanonetwork fabrication reproducibility, robust stability, triggered drug release in a controlled fashion, and cell-selective cytotoxicity behavior, we believe, will make the system a potential candidate in the development of robust materials for chemotherapeutic applications.

Co-delivery of curcumin and epigallocatechin gallate in W/O/W emulsions stabilized by protein fibril-cellulose complexes

Hydrophobic curcumin and hydrophilic epigallocatechin gallate (EGCG) are reported to exhibit a variety of biological activities and may exhibit synergistic effects when used in combination. A co-encapsulation system was developed to improve their applicability and bioavailability. This delivery system consisted of a water-in-oil-in-water (W1/O/W2) double emulsion stabilized by whey protein isolate fibrils (WPIFs) and cellulose nanocrystals (CNCs). Double emulsions were fabricated using a two-step emulsification method using either WPIF-CNC complexes or WPIF alone. The physicochemical stability, encapsulation performance, and digestive properties of the delivery systems were then investigated. The double emulsions stabilized by the WPIF-CNC complexes were more resistant to heat and salt stress, exhibited greater encapsulation stability, and had a higher bioaccessibility for curcumin (67.8%) and EGCG (68.9%) than those stabilized by WPIFs. This research shows that the stability and bioaccessibility of curcumin and EGCG can be enhanced by co-encapsulating them in emulsion-based delivery systems using nanostructured protein-polysaccharide complexes.

Development and optimization of RofA-PAMAM dendrimer complex materials for sustained drug delivery

The current indagation is observed on anatomization of drug release dynamics aiming at preceding the anti-inflammation activity of roflumilast analogue (RofA), post assemblage with dendrimer. The study pondered the development and optimization of RofA-Dendrimer complexes for sustained delivery. Many physiological and physicochemical factors, like encapsulation potency, drug loading, drug release, dissolvability, stability, and anti-inflammation activity were determined. The study results in the observation of encapsulation potency of 72.64 ± 0.189%, loading capability of 63.41 ± 0.166%, and in vitro release of RofA-4.0 G PAMAM complex was found to increase making it an appropriate candidate for sustained drug release progressively . It has been found by constructing drug release kinetics, that the release followed was a non- fickian diffusion ( n<0.89). The phase solubility diagram indicated an increment of solubility of RofA with varied dendrimer generation as well as pH. The study was initiated to prepare a suited candidate fit to scale back site-specific inflammation. Further concluding the contemplation for its sustained delivery, enhanced solubility, and potentially augmented anti-inflammation activity. • The aims of this study were to assess the potentially augmented effect of roflumilast analogue post assemblage with different generations of polyamidoamine dendrimer. • In this study, RofA-Dendrimer complexes, with RofA as the model drug, the encapsulation potency, loading capacity, in vitro drug release behavior, and stability were studied. • The effect of the concentration of varied generations of PAMAM dendrimer on the solubility of RofA has been discussed in detail. • We also examined the anti-inflammatory activity and sought to determine the inhibitory effect on protein denaturation. • The results were affirmed by the determination of n value i.e. hill coefficient with respect to the substrate concentration and rate of inhibition. • The impact of adjuvant antibiotic therapy was also explored by the means of a cytotoxicity assay.

Liposomes for encapsulation of liposoluble vitamins (A, D, E and K): Comparation of loading ability, storage stability and bilayer dynamics

To understand the encapsulation difference and stability mechanism of nanoliposomes (NLPs) loaded with different kinds and loads of liposoluble vitamins (LSV, including VA, VD, VE, and VK), the physicochemical stability during three-months storage and bilayer membrane properties of LSV-NLPs were evaluated. The results suggested that VD and VE were not suitable for high-load (≥30 wt%) encapsulation, but the stability of other LSV-NLPs was excellent during storage. Their particle size was less than 100 nm, the polydispersity index was less than 0.3, and the retention rate of VE and VK remained above 85 %. LSV encapsulation inhibited malondialdehyde production, decreased liposome surface roughness, and improved nanoliposome rigidity. The order of occupying capacity of LSV to the hydrophobic zone of the bilayer was VK＞VD＞VE＞VA, and the stability of LSV located in the hydrophobic region was better. Except for high-load VD and VE, the other LSV encapsulation increased the microviscosity of the lipid-water interface and hydrophobic zone by 0.5 ∼ 7.1 times and 0.5 ∼ 20 times, respectively. The accumulation of acyl chain was enhanced by 0.2 ∼ 4 times, and the interchain longitudinal and intra-chain transverse order degree was increased by 10.89 %∼144.35 % and 3.26 %∼115.52 %, respectively. High microviscosity and tight chain stacking limited bilayer fluidity and thus improve LSV-NLPs stability. This work will contribute to the application of nanoliposomes as liposoluble vitamin carriers in the food industry.

Citral Essential Oil-Loaded Microcapsules by Simple Coacervation and Its Application on Peach Preservation.

Citral essential oil (CEO) was encapsulated by the single coalescence method, and its stability, release properties, and ability to maintain freshness were evaluated for the first time. The microshape characteristics of a CEO-loaded microcapsule (CM) were analyzed by inverted microscopy (OM) and scanning electron microscopy (SEM). The encapsulation efficiency, stability, and release behavior of CEO were evaluated using Fourier transform infrared spectroscopy (FTIR), thermogravimetric/differential thermal comprehensive analysis (TG/DSC), and gas chromatography mass spectrometry (GC/MS). Moreover, peaches were used to evaluate the preservation properties of the CEO-loaded microcapsule. The results showed that the microcapsule produced using simple coacervation had better microstructure and the ability to reduce and control the release of citral essential oil. The qualities of peaches, such as appearance changes, hardness, soluble solid content, total acids, and total bacterial counts, were significantly improved in the CM system during storage, in comparison with the control and cold storage groups. Therefore, the CM has potential applications and development prospects in the food, drug, and other industries.